Extracting Biologically

active substances from

Plants Haruna KobayashiYohei NosakaYuta FukushimaEriko Minamiya

Koshi High School

We listened to our seniors’ study “Making chemical-free fertilizer with plant components” and we will extract component from plants to find good applications for our life.

~Motive~

CinnamonCork TreeBracket fungus ChagaRooibos

~Research Material~

・ Spice・ We say it has hyperthermic action.

Cinnamon

Cork tree ・ Rutaceae

Rooibos・ we say it is a medicine of perpetual youth and long life・ It has antioxidant action

・ Shelf-type mushrooms・ It is born in the stem of natural trees, fallen trees, and dead trees

Bracket fugus

・ Cold-resistant mushroom・ It is born in cold areas

Chaga

Bracket fugus and Chaga are relatives. But both of them are different in trees.

Do they have different components!?

Crush material with ethanol using a machine.Leave it for three weeks. Concentrate it.Add water to it, to get n-Hexane Extracts.

～ First Experiment ～

①Make 10ml of solution which is 90% Chloroform and 10% Methanol (we will just call it “the solution” after this), and make a sample with the solution.

～ Method ～

Vacuum Filtration

Concentration

～ Thin Layer Chromatography(TLC) ～

②Spot the bottom of the TLC plate with the concentrate from step .① Then, we put 5ml of the solution into a glass case and we put the TLC into the case.

③After the solution rises enough, check if you can see component spots with UV light.

…Spots of components （ A thicker mark means the component is more concentrated. ）

…The first spot

～ Results of Experiment ～

Ⅰ Cinnamon Ⅱ Rooibos Ⅲ Bracket fugus

Ⅳ 　 Cork tree,　　 Chaga

The line of rising solution

～ Column Chromatography ～

① Make 200ml of the solution ② Put Silica gel and the solution into an Erlenmeyer flask and mix them.

～ Method ～

ChloroformMethanol

Silica 　gel

③ Put into a column and pour in a little solution.② After that we put 5ml of the solution mixed with a sample into the Column .

④ Put a little Silica gel on and pour ③ little by little another 200ml of the solution.

→ Silica gel

→Sample

→Solution

→Expand

Mixed Silica gel and 5ml solution

⑤ Divide the liquid into 2 equal parts, put in a Flask and concentrate them.

Result of Experiment

  Color FragranceC －０ strong

red 

C －１ Yellow Weak C-0 smellC －２ Yellow Strong C-0 smellC －３ Reddish

brown 

Ⅰ:Cinnamon

Ⅱ:RooibosＲ－０

Ｒ－１ Ｒ－２

  Color Fragrance

R －０ Strongyellow-green

 

R －１ Weak yellow-green

 

R －２ WeakYellow-green

 

Ⅲ ： Bracket fungus Ｓ－１ Ｓ－２

Ｓ－３ Ｓ－４ Ｓ－５

※There is not S-0 picture.

  Color FlagranceS －０

Yellow-green  

S －１

Strong yellow  

S －２

Weak yellow S-0 smell

S －３

Weak yellow Sour smell

S －４

Weak yellow Sour smell

S －５

Weak yellow  

Ⅰ Ⅱ Ⅲ

…Spot of components （ a thicker mark means the spot is more concentrated ）

……The first spot

C－

１

C－

２

C－

３

R－１

R－２

C－０

R－０

S－１

S－０ ,２～５

～ Consideration ～・ C-2 has a strong C-0 smell ⇒We think C-2 has fragrance components.・ C-3 has C-0 color⇒We think C-3 has color components.

・ R-2 is like C-0 color ⇒We think R-2 has color components.

Cinnamon

Rooibos

Bracket fugus・ S-2 is like S-0 smell⇒We think S-2 has fragrance components.

～ Future Activities ～

・ Try to do other experiments.

・ Try to extract component of other plants.・ Conduct experiments to test the effects of components.

We will examine whether Rooibos have inhibits UV light and so on.

Thank you f r　 listening

o