EXPERIMENTAL DESIGN EXTRACTION OF LIMONENE FROM

ORANGE PEELS

This case of steam distillation is used to isolate

the essential oil of limonene from the orange

peels. Orange peels are cut into pieces, and

placed into water. The peel of oranges are boiled

in water and the oil produced (limonene) distilled

in steam at a temperature just below 100oc, well

below its normal boiling point. The immiscible oil

can then be separated.

 

MODEL SYSTEM: invivo

Rats will be injected with carcinogen continuously until a large tumor mass is detected. Rats will be placed on about 10 percent limonene for 21 weeks. EXPECTED RESULTLimonene will cause about 80 percent inhibition of mammary tumors.

Carcinogen in Albino Rats

A B

EXPECTED RESULT IN HUMANS

The rat metabolism of limonene closely parallels that of humans in terms of both the specific metabolites formed and the time in which they formed. These results suggest that limonene will have similar cancer treating benefits in humans.‑

limonene inhibits cancer formation by increasing the levels of phase II liver enzymes that bind a wide variety of carcinogens to form less toxic conjugates that are readily eliminated by the digestive and respiratory systems.

REFERENCES 1.American Society of Nutrition. “Prevention and Therapy of Cancer by Dietary Monoterpenes.”Journal of Nutrition. N.P. 2012 Web.<http://www.jn.nutrition.org/content/129/3/775S.full> 26 Sept 2012. 2.Margo Upson. “Causes of Tumors”. Ed. Bronwyn Harris. N.P. 16 July 2012.Web <http://www.wisegeek.org/what-causes-tumors.htm> 25 Sept 2012

3.Young Life Research Clinic.” Lmonene and Tumor”. Young Living Research. P.134. 18 Sept 2012. Web < http://www.younglivinglink.com/?p=134> 26 Sept 2012.4.Memorial Sloan-Kettering Cancer Center. “About Herbs, Botanicals & other products”. Integrative Medicine. N.P. Aug 2010. Web < http://www.mskcc.org/cancer-care/herb/d-limonene> 28 Sept 2012.