Experiment 6Cultivation and Identification of Virus

Culture of virus Culture in experimental animal Culture in chicken embryo Culture in cell: *primary cultural cells *diploid cell strain *continuous or infinite cell line of strain

Culture in experimental animal

Culture in chicken embryo

Culture in cell: *primary cultural cells *diploid cell strain *continuous or infinite cell line of strain

*Identification in cell culture 1) Cytopathic effect (CPE ) : 2) Inclusion body 3Hemadsorption 4Interference with formation of a CPE by a second virus

CPE

Negri body in a neuron

Hemagglutination test and Hemagglutination inhibition test

1.Hemagglutination testhemagglutinin HA the glycoprotein spike encoded by the viral genes .Adsorption and enter into the host cells with the virus. HA can bind with N-acetyl neuraminic acid enzyme receptor from erythrocyte of human or other animals and cause hemagglutination.

Principles of Hemagglutination inhibition test hemagglutinin Phenomenon caused by virus can be inhibited by corresponding anticoagulant antibody,this is called hemagglutination inhibition. hemagglutinin specific antibodies interact with corresponding HA of virus surface ,so the phenomenon that inhibit virus hemagglutinin is called hemagglutinin inhibition test.

CPE inclusive bodybaozhi