Experience in wpu mouselab revised
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Transcript of Experience in wpu mouselab revised
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Mara KettleRocheSummer 2012
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Skills Learned• General Mouse Lab Procedure
and Skills-including husbandry• Experimental Preparation• Experimental Design• Data Collection and Analysis• Scientific Literature• Professional Social Skills
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General Mouse Lab Procedure and Skills Proper Handling of Mice
Maintenance Sexing
Behavioral Tests Formalin Test Hot Plate Test Social Interaction
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Experimental Preparation* Observed an on-going pain experiment
Formalin Pain Test and Social Interaction
Preliminary Literature Search Pubmed Academic Search Complete
(EBSCOhost.com) Helped Design an Experiment
Short Term Pain Study-Major Focus of my Work
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Hypothesis
Stress decreases pain response because it triggers a pain suppression response called SIA
Necessary Components Aversive Stimuli: Stressors:
Restraint (severe) Noxious Pain Stimuli: Pain Test: Hot
Plate
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What is SIA?
“a phenomenon of pain suppression that occurs upon exposure to stressful stimuli”1
Accidents
Wars/Battles
Trauma
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Experimental Design
16 Male Mice: BTBR T+ tf/J (n=8) C57 BL/6J(n=8)
Restraint Stress
Hot Plate Test
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Strains of Mice Used
BTBR C57•An autistic model•Show reduced social interactions, impaired play, low exploratory behavior, and high anxiety
• Used as a control/general purpose strain •Breeds well, long life-span
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Experimental Design (cont.)1. Pre-stress pain test
2. Give mice stress Restraint stress for an hour in clear 50ml
centrifuge tubes
3. Post-stress pain test
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Experimental Methods• Pre Stress Testing
• Cages were housed outside testing room
• Mice were placed onto hotplate heated 53°C and latency until first pain behavior was measured
• Pain latency was recorded and procedure was repeated until all mice were tested
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Restraint Stress
• Method • 50ml centrifuge tubes
• 1 hour duration
• Post-stress testing began immediately following
• post stress pain latency time recorded
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Results
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P<0.05
P<0.05
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Results cont…
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Conclusion:
Restraint stress increases hotplate latencies in C57 and BTBR mice
It appears that there is a strain by treatment effect ;C57 mice demonstrate a greater stress induced analgesia”
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References
1 Butler RK, Finn DP. Stress-induced analgesia. Prog Neurobiol. 2009 Jul;88(3):184-202. Epub 2009 Apr 22. Review. PubMed PMID: 19393288.
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Acknowledgements
Roche ProgramWilliam Paterson UniversityDr. WeissMr. Norman SchanzDr. BennoAbbie Post (mentor) Joon Ho Seo (consultant)
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Thank You