Exocytosis and Endocytosis - CellBiology · Single molecule uncoating assay clathrin hsc70 overlay...
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Transcript of Exocytosis and Endocytosis - CellBiology · Single molecule uncoating assay clathrin hsc70 overlay...
http://dx.doi.org/10.1016/S0092-8674(03)01079-1
Exocytosis
Transcription
Endocytosis
Phagocytosis: Neutrophil
Caveolae-lipid recycling -signalling -endocytosis
Peroxisomes- fatty acid metabolism - oxidative reactions (H2O2), e.g. fatty acids, cholesterol, bile salts
Clathrin mediated endocytosis
Massol et al PNAS 2006
Live cell imaging
Heuser 1989
Xing; Böcking; Wolf; Grigorieff; Kirchhausen; Harrison, EMBO J 2010Rapoport, Boll, Yu, Böcking, Kirchhausen, Mol Biol Cell 2008
Single particle uncoating assay
Time
Intensity
2. coupled release
Time
Intensity
1. uncoupled release
Single molecule uncoating assay
clathrin hsc70 overlay
Nature Structural and Molecular Biology, 2011, 18(3), 295-301
Janet Iwasa and Tom Kirchhausen,Cell Biology, Harvard Medical School
Cellular Mechanisms of Health and Disease
SOMS3232
Research internships and Honours projects
Molecular Machines Group
