Amyloid Histology Stain for Rapid Bacterial Endospore Imaging
Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the...
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Transcript of Ex. 5: Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the...
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Ex. 5: Acid-Fast and Endospore Stains
Objectives:Explain the rationale and procedure for the acid-fast and endospore stains
Perform and interpret the acid fast and endospore stains
Review the structure and function of endospores
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For Acid Fast Bacteria
Use only
Carbol fuchsin for AFB!!
Acid Alcohol
MethyleneBlue
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Modified Kinyoun’s Acid-fast Stain – Cold Method
• Heat fix smear
• Primary stain: Carbol fuchsin for AFB, (5 min)
• Rinse with water
• Decolorize briefly (~10 drops) with acid alcohol (~ 30 sec)
• Immediately rinse with water
• Counterstain: Methylene blue (1 min)
• Rinse with water
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Endospore Stain
• Heat fix smear
• Primary stain: Malachite green, steam (5 min)
• Rinse thoroughly with water (~ 30 sec)
• Counterstain: Safranin (1 min)
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