EWMA 2014 - EP448 BACTERIA AEROSOL SPREAD WITH DIFFERENT METHODS FOR WOUND DEBRIDEMENT

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BACTERIA AEROSOL SPREAD WITH DIFFERENT METHODS FOR WOUND DEBRIDEMENT HH Sönnergren, MD, LLB, S Polesie, MD, L Strömbeck, PhD, F Aldenborg, MD, PhD, BR Johansson, MD, PhD, J Faergemann, MD, PhD Department of Dermatology and Venereology Institute of Clinical Sciences at Sahlgrenska Academy, University of Gothenburg Background •Debridement is essential in wound treatment to remove necrotic tissue and wound bacteria •Wound debridement may lead to the transmission of bacteria by aerosolization and cross-contamination of bacteria to other patients Purpose The aims of the study were to in an ex vivo porcine wound model; 1) Determine the reduction of wound bacterial load, and 2) Compare the amount of bacterial aerosolization induced by debridement, using; a) Cold steel curette, b) Plasma-mediated bipolar radiofrequency ablation, or c) Hydro-debridement 1 of 4

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Sönnergren Henrik H., Polesie Sam, Strömbeck Louise, Aldenborg Frank, Johansson Bengt R., Faergemann Jan

Transcript of EWMA 2014 - EP448 BACTERIA AEROSOL SPREAD WITH DIFFERENT METHODS FOR WOUND DEBRIDEMENT

Page 1: EWMA 2014 - EP448 BACTERIA AEROSOL SPREAD WITH DIFFERENT METHODS FOR WOUND DEBRIDEMENT

BACTERIA AEROSOL SPREAD WITH DIFFERENT METHODS FOR WOUND DEBRIDEMENT

HH Sönnergren, MD, LLB, S Polesie, MD, L Strömbeck, PhD, F Aldenborg, MD, PhD, BR Johansson, MD, PhD, J Faergemann, MD, PhD Department of Dermatology and Venereology Institute of Clinical Sciences at Sahlgrenska Academy, University of Gothenburg

Background • Debridement is essential in wound treatment to remove necrotic tissue and wound bacteria • Wound debridement may lead to the transmission of bacteria by aerosolization and cross-contamination of bacteria to other patients

Purpose The aims of the study were to in an ex vivo porcine wound model; 1)  Determine the reduction of wound bacterial

load, and 2)  Compare the amount of bacterial

aerosolization induced by debridement, using;

a)  Cold steel curette, b)  Plasma-mediated bipolar radiofrequency

ablation, or c)  Hydro-debridement

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Methods

Porcine full thickness skin specimens

Artificial full thickness wounds cut out

Inoculated with S. aureus and incubated 24 hrs

Debrided with Plasma-ablation/Hydrosurgery/Curette

Bacteria-aerosol measured during debridement and Wound swabs pre/post

Fig 1. Artificial porcine wound.

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Results

•  Only plasma-ablation significantly reduced the

wound bacterial load (P<0.0001).

•  Hydro-debridement resulted in a significant bacterial aerosolization increase with up to 20 000% (P<0.001).

•  Plasma-ablation and curette debridement did not increase bacterial aerosolization.

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Conclusions

This study shows that plasma-ablation is a promising wound debridement method which; •  Effectively reduce the wound bed bacterial

load. •  Do not increase the risk of bacterial

aerosolization.

Take home message The plasma-ablation method should be further evaluated in prospective clinical trials to evaluate the clinical antibacterial effect and the wound healing progression after treatment.

For additional information, please contact: Dr Henrik H Sönnergren Physician Department of Dermatology Gröna stråket 16 SE-41345, Gothenburg Sweden Phone: +46 703 347532 [email protected]

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