EU COST T1002 action WG meeting, Lamorlaye, France 10-13 May...

RC for Microsystems/ Nanotechnology, Kaunas TU, Lithuania

EU COST T1002 action WG meeting, Lamorlaye, France 10-13 May 2011

Established in 1998

to act as a focus for interdisciplinary research into microsystemsand nanotechnology.

The Center aims to stimulate nanoscience and MST activity in Lithuania by participatingin European and global networks, research projects and by the information dissemination.

Contact: Prof. Valentinas Snitka, [email protected]: http://nano.ktu.lt

Research Center for Microsystems and NanotechnologyKaunas University of Technology, Lithuania

mailto:[email protected]

http://nano.ktu.lt/



Valentinas Snitka

Head of Research Center for Microsystems and Nanotechnology, Kaunas TUProfessor in Nanotechnology

Other activities :

- Serving EC as PTA in Nanobiotechnology (8 projects)- Coordinator of FP7 NANOMAT project ( http://www.nanomat-lt.eu)- CEN/TC 352 Nanotechnology expert

- EU Nanosafety cluster member

- FP6 NMP committee member

- EU CREST committee member

http://www.nanomat-lt.eu/





Main topics:

• New SPM methods and development of next generation

nanoscale imaging probes and instrumentation

(STM, AFM, SNOM, s-SNOM, tip-enhanced Raman spectroscopy and imaging)

• Bionanotechnology and subcellular imaging

• Nanotoxicity



Current projects :

EU FP7 Center of Excellence for Nanostructured Materialswith the twinning partners : Centre for Nanoscience and Quantum Information, University of Bristol;

Micro and Nanosciences Laboratory, Aalto University, Helsinki;

National Centre for Microelectronics, Barcelona.

EU FP7 "Development of sustainable solutions for nanotechnology-based

products based on hazard characterization and LCA"



Expertise

Nanoinstrumentation (design, prototyping)- nanopositioning, piezoatuators,- nanoresolution displacement sensors- Home built SPM, photon counting

Scanning probe microscopy for materials research, manipulation- AFM, Biological AFM, STM, SNOM, TERS, SERS

Sol-gel ferroelectric PZT films for sensors and actuators- Piezo-AFM, polarization nanolitography

New materials for sensors, membranes and microoptics (ALD, CVD)- Carbon nanotubes, graphene

Nanoparticles physical-chemical characterization

TiO2, ZnO, CNT, Cellulose, graphene



Nanopositioning (subnano)

Finite Element Modelling

Design

Prototyping

V.Snitka Ultrasonic actuators for nanopositioning, Ultrasonics, 38(1-8), 2000, 20-25.



Finite Element Modelling Laser micromachining

V.Lendraitis, V.Snitka et all. Fabrication of actuator for nanopositioning using laser

micro-machining, Microelectronic Engineering 83 (2006) 1212–1215



Home built SPM instrumentation

SNOM, Photon counting BioAFM / SNOM

Ulcinas A, Snitka V Intermittent contact AFM using the higher modes of weak cantilever

Ultramicroscopy 86 (1-2): 217-222 JAN 2001



Ferroelectric films for sensors and actuators

-0.7

-0.5

-0.3

-0.1

0.1

0.3

0.5

0.7

-10 -5 0 5 10

Priešįtampis, V

Pje

zo

ats

ak

as

, s

.v.

PSZT

PST/PSZT

PZT

50

90

130

170

210

250

-10 -5 0 5 10

Priešįtampis, V

Fa

zė

, la

ips

nia

i

PSZT

PST/PSZT

PZT

A.Ulcinas, V.Snitka, M.ES-Souni Investigation of microstructure and piezoelectric properties of Zr- and Sm

doped PbTiO3 nanostructured thin films derived by sol–gel technology, Sensors and Actuators B 109 (2005)

97–101

Piezo-AFM



Write-read mode: memory, Polarization litography

Area: 1x1 mkm

V.Snitka, A.Ulcinas, et all AFM based polarization nanolithography on PZT sol–gel films,

Microelectronic Engineering 83 (2006) 1456–1459



New scanning probe microscopy instrument particularly suited for nanoscale investigations of biomolecular structures and cells in their native environment (Collaboration with Bristol Univ.)

M. Antognozzi et al., Nanotechnology 19 (2008) 384002

Optical assembly of new SPM instrumentShear force head and detection branch

Control software interface window (host computer)

. Key results: versatile multimodal SPM

platform for mechanical and optical

investigation of nanoscale properties

of biomolecular structures built;

reconfigurable electronic controller and

general purpose SPM software developed.



Control electronics of new SPM instrument and auxiliary electronic devices.

Optical microscopy image of the surface and vertically oriented shear force probe showing laser scattering from dust particle on the surface and from the end of the probe

Reconfigurable FPGA-based controller is developed.



Electroporation for drug delivery

= E r cos



Electroporation for drug delivery

G.Saulis et all, Determination of cell electroporation in small-volume samples by

using a mini potassium-selective elektrode, Analytical Biochemistry 345 (2005) 340–342

A . KONTROLĖ mikrofotografija B. kontrolė po fluorescentiniu mikroskopu

po šviesiniu mikroskopu

C. Po elektroporacijos E = 800 V/cm, D. E = 1200 V/cm, = 1ms.

= 1ms

F. E = 1400 V/c, = 1ms G. E = 1600 V/cm, = 1ms

4 pav. Hepatomos MH22 ląstelių švytinčių, dėl fluorescuojančių liucifer yellow

(LY), kiekio priklausomybė nuo elektrinio lauko stiprio E ir impulso trukmės .

Suspensijos imties dydis – 6 l visais atvejais toks pat.

Labeled with Liucifer

yellow



Examples of the resent research results

AFM two dimensional images of the mouse hepatoma MH22a surface: (A) prior to

the exposure to electric pulses and after the exposure to (B) 0.8 kV/cm and (C) 1.6

kV/cm intensity electric square-wave pulses with the duration of 2 ms. Scanning

area: (A) 7 x 7 μm2; (B) 8 x 8 μm2 and (C) 7 x 7 μm2.

2a 2b 2c

The influence of electroporation on cells membrane damage and cells viability



Synthesis of gold Quantum dots for SERS and

biosensing

440 480 520 560 600 640 680 720 7600,0

0,5

1,0

1,5

2,0

2,5

3,0

3,5

4,0

4,5

5,0

PL

in

ten

syvu

ma

s(s

.v.)

Bangos ilgis (nm)

zad 330nm

zad 340nm

zad 360nm

zad 380nm

zad 400nm

zad 405nm

zad 420nm

zad 440nm

zad 460 nm

zad 480 nm

zad 500 nm

Ramanaviciene A, Nastajute G, Snitka V, et al. Spectrophotometric evaluation of gold nanoparticles as red-ox

mediator for glucose oxidase Source: SENSORS AND ACTUATORS B-CHEMICAL 137 (2), Pages: 483-489 ,2009



AFM – Micro-Raman integration

TERS, chemical mapping



TERS Inverted configuration

V.Snitka et all. Novel gold cantilever for nano-Raman spectroscopy of graphene, Microelectronic Engineering,2011

doi:10.1016/j.mee.2011.02.046



Yast cells transformants investigation

by AFM , Raman

Newly created heterologous expression plasmids were introduced into

S. cerevisiae 21PMR ura3-52 leu2 strain and the transformants were

selected for uracil or leucine auxotrophy.



Optical image of yeast cells on glass substrate and NSG11

cantilever (yellow)

AFM topography image of yeast cells on glass substrate

(50x50 μm)



0 5 10 15 20 25 30 35

0

500

1000

1500

2000

2500

3000

Fre

qu

en

cy, a

rb. u

nits

Size, nm

non-modified

modified (1)

modified (2)

non-modified modified (1)

modified (2)



-15 -10 -5 0 5 10 15

0,0

0,5

1,0

1,5

2,0

2,5

3,0

3,5

Ma

gn

itu

de

, n

ADistance, nm

non-modified

non-modified died

modified (2)

-10 -5 0 5 10 15 20 25

0,0

0,1

0,2

DF

L, n

A

Distance, nm

non-modified

modified (1)

modified (2)

Analysis of elastic properties



1800 2000 2200 2400 2600 2800 3000 3200 3400

0

200

400

600

modified

29

30

29

05

28

70

Ra

ma

n s

ign

al

Raman shift, 1/cm

non-modified

0 200 400 600 800 1000 1200 1400 1600 1800 2000 2200

-600

-400

-200

0

200

400

600

800

Ra

ma

n s

ign

al

Raman shift, 1/cm

18

75

16

55

14

5512

85

11

85

11

00

89

0

72

0

56

0

51

5

non-modified

modified

Acquisition time is 60s, laser power is 7mW, excitation wavelength is 632.8nm.

Yeast cells transformants Raman spectra



Si barcode inside the cell, chem maping



Contribution to bring to the Action

WG 3: Integrated biological AFM, Confocal fluorescence

and tip-enhanced Raman microscopy for biomaterials,

pharmacological and cell research.

WG 4: The nanoparticles characterization by AFM and

TERS , SERS



THANK YOU !

nano.ktu.lt

[email protected]

http://www.microsys.ktu.lt/

EU COST T1002 action WG meeting, Lamorlaye, France 10-13 May...

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