ETHOSOMES AS NOVEL DRUG DELIVERY SYSTEM

Presented by Ms. SHIKHA Y. SINGH Under the guidance of Prof. SMITA.S AHER

Kalyani charitable trust’sRavidra gambhirrhao Sapkal

Anjaneri, Nashik-422213

Presentaion by - Shikha Y Singh

CONTENTS INTRODUCTION ETHOSOMES COMPOSITION MECHANISM OF ACTION ADVANTAGES & DISADVANTAGES CHARACTERIZATION METHODS OF PREPARATION APPLICATION CONCLUSION

Presentaion by - Shikha Y Singh

INTRODUCTION

Why we need NDDS?What is NDDS?What are the approaches used in

NDDS?Why TDDS ?What is vesicular system?What is Ethosomes?

Presentaion by - Shikha Y Singh

ETHOSOMES Ethosomes are “Ethanolic liposomes”. Ethosomes were developed by touitou, 1997 Ethosomes are non-invasive delivery carrier

that enable drugs to reach the deep skin layers and / or systemic circulation.

“Soft vesicles” represent novel vesicles carrier for enhanced delivery through the skin.

Size of ethosomes vesicles-30nm to few microns.

Presentaion by - Shikha Y Singh

COMPOSITION

It mainly comprises ofPhospholipid- PC, PA, PPG, PS

etcAlcohol- ethanol & isopropyl

alcoholWater

Polyglycol or glycol

Cholesterol

Dye- rodamine & FITC

Vehicle - Carbapol

Presentaion by - Shikha Y SinghSKIN MECHANISM AFTER DRUG APPLICATION

Presentaion by - Shikha Y SinghSKIN MECHANISM AFTER DRUG APPLICATION

Presentaion by - Shikha Y SinghETHANOL PENETRATION

Presentaion by - Shikha Y SinghADVANTAGES:

Ethosomes enhance permeation of the drug through skin transdermal & dermal delivery.

Ethosomes are platforms for the delivery of large & diverse groups of drugs (peptides, protein molecules)

Ethosomal systems are much more efficient at delivering a fluorescent probe (quantum dots) to the skin in terms of quantity & depth.

Low risk profile- the technology has no large scale drug development risk, as the toxicological profiles of the ethosomes components are well documented in the scientific literature.

High patient compliance- the ethosomes drugs are administered in a semisolid form (gel/cream), producing high patient compliance. In contrast, iontophoresis & phonophoresis are relatively complicated to use, which will affect patient compliance.

High market attractiveness for products with proprietary technology. Relatively simple to manufacture with no complicated technical investments required for the production of ethosomes.

The ethosomes system is passive, non-passive & available for immediate commercialization.

Presentaion by - Shikha Y SinghLIMITATIONS: Poor yield. In case if shell locking is ineffective then the ethosomes may

coalescence and fall apart on transfer into water. Loss of product during transfer form organic to water media.Future perspective: Introduction of ethosomes has intiated new area in transdermal

drug delivery Further research in this area will allow better control over drug

release in vivo allowing physicians to make therapy more efficient. It offers good opportunity for non-invasive delivery of small-,

medium- & large-sized drug molecules. Special emphasis given to skin delivery of proteins & other

macromolecules & for transcutaneous immunization.

Presentaion by - Shikha Y SinghCHARACTERISATION METHODS:

Vesicle shape- Transmission electron microscopy (TEM), Scanning electron microscopy( SEM) Entrapment efficiency- Mini column centrifugation method; Fluorescence spectrophotometry Vesicle size- Dynamic light scattering methodVesicle Skin interaction study- Confocal laser scanning microscopy; Fluorescence microscopy; TEM.Eosin-Hematoxylin stainingPhospholipid-ethanol interaction- 31P NMR; Differential scanning calorimeter

Degree of deformability- Extrusion methodZeta potential- Zeta meterTurbidity- NephalometerIn vitro drug release study- Franz diffusion cell with artificial or biological membrane, Dialysis bag diffusion Drug deposition study- Franz diffusion cellStability study- Dynamic light scattering method & TEM

Presentaion by - Shikha Y SinghMETHOD OF PREPARATION

Phospholipid + Drug + Other lipid material

Dissolve in Ethanol in covered vessel with vigorous stirring at room temperature

Add Propylene Glycol at 40°C during stirring

Heat mix upto 30°C

Add water at 30°C

Stir for 5 minuets in covered vessel

Size reduction by sonication or extrusion

Store under refrigeration

COLD METHOD

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Disperse Phospholipid in water at 40°C

Ethanol + Propylene Glycol at 40°C

Mix organic phase to aqueous phase

Add drug dissolved in suitable solvent (Water or Ethanol depending on solubility)

METHOD OF PREPARATION

INJECTION METHOD

CLASSIC DISPERSION METHOD

HOT METHOD

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APPLICATION

Delivery of Antiviral drugs Topical delivery of DNA Transdermal delivery of Hormones. Delivery of Anti-parkinsonism agent Transcellular Delivery Delivery of Anti-Arthritis Drugs Delivery of Problematic drug molecules Delivery of Antibiotics Delivery of Antigen loaded Drugs Delivery of NSAIDS Widely used in Cosmoceuticals

Presentaion by - Shikha Y SinghCONCLUSION The main limiting factor of transdermal drug

delivery system i.e. epidermal barrier can be overcome by ethosomes to significant extent.

The ethosomes more advantages when compared to transdermal and dermal delivery.

Ethosomes are the non invasive drug delivery carriers that enable drugs to reach the deep skin layers finally delivering to the systemic circulation.

It delivers large molecules such as peptides, protein molecules. Simple method for drug delivery in comparison to Iontophoresis and Phonophoresis and other complicated methods.

High patient compliance as it is administrated in semisolid form (gel or cream) and various application in Pharmaceutical, Veterinary, Cosmetic field.

Presentaion by - Shikha Y Singh

ANY QUERIES???

Presentaion by - Shikha Y Singh