Seismic Design and Analysis of Underground Estructures - Hashash, Hook, Schmidt y Yao
Estudi de les estructures G‐quadruplex e i‐motif del DNA ... · Yaku et al., 2012....
Transcript of Estudi de les estructures G‐quadruplex e i‐motif del DNA ... · Yaku et al., 2012....
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Estudi de les estructures G‐quadruplex e i‐motifdel DNA mitjançant tècniques espectroscòpiques i quimiomètriquesDR. RAIMUNDO GARGALLO
UB.EDU/GESQ /DNA
DEPT. D ’ENGINYER IA QUÍMICA I QUÍMICA ANAL ÍT ICA
UNIVERS ITAT DE BARCELONA
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AcknowledgmentsFormer PhD students: Dra. Montserrat Vives i Dr. Joaquim Jaumot
PhD Student: Sanae Benabou
Many MSc and Graduate Students
Collaborators: ◦ IQAC: Dr. Ramon Eritja, Dra. Anna Aviñó, Dr. Santi Grijalvo, Dra. Sonia Pérez‐Rentero, Dr. Rubén Ferreira …
◦ IQFR “Rocasolano”: Dr. Carlos González◦ IBMB: Dr. Sébastien Lyonnais, Dra. Maria Solà …◦ Masaryk University (Brno, Czech Republic): Dr. Petr Taborsky and many MSc and PhD students◦ …
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Overview
• Introduction to G‐quadruplex and i‐motif structures• Fundamentals• In vivo• Applications
•Multivariate data analysis methods and examples
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G‐quadruplex structures. G‐tetrad
Neidle, 2016M = K+, Na+, NH4+, …
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Zhu et al. 2013
G‐quadruplex structures. Topologies and loops
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G‐quadruplex structures. Polymorphism
Marsh et al., 1995
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Circular Dichroism NMR
220 240 260 280 300 320
-5
0
5
10
15
20
Wavelength/nm
Ellip
ticity
/mde
g
ckit1T21ckit1T18SMG03T21
Kah Wai Lim et al., 2015Benito et al., in preparation
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ESI‐MS
Size‐Exclusion Chromatography
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G‐quadruplex structures. In vivoGuanine‐rich sequences are present in:
‐ End of telomeres
‐ Human telomere: (T2AG3)n‐ Potential role in cellular replication
‐ Near the promoter regions of several genes (c‐myc, c‐kit, KRAS, bcl‐2, ...)
‐ They are believed to control gene expression:
Yaku et al., 2012
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G‐quadruplex structures. Applications
‐ Sensors for metal ions quantitation
‐ Fluorescence‐based methods
‐ Catalysis
‐ Drug delivery
‐ … Ueyama et al., 2002Takenaka and Juskowiak, 2011
Zhou et al., 2015
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Benabou et al. 2014
i‐motif structures. Fundamentals
As pKa cytosine ~ 4.5, pH control is essential!!
pKa cytosine ~ 4.5
Not very stable at pH 7 and 37oC
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i‐motif structures. Ligands
Kang et al. 2014
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i‐motif structures. Chemical modifications
acyclic threoninol cytidinePérez‐Rentero et al. 2015
(2’S)‐2’‐deoxy‐2’‐C‐methyl‐cytidineAviñó et al. 2017
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Many applications:
‐ FRET‐based pH‐sensing devices
‐ Stabilization of gold and silver nanoclusters and
nanoparticles
‐ pH‐fuelled nanomotors
Sharmaet al. 20
07
i‐motif structures. Applications
Xia et al. 2008
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Overview• Introduction to G‐quadruplex and i‐motif structures
• Fundamentals• In vivo• Applications
•Multivariate data analysis methods and examples
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Spectroscopically‐monitored unfolding of an i‐motif
H0 (kcal/mol)= -52 + 2 (95%)S0 (cal/K·mol)= -169 + 6 (95%)Tm (oC)= 32.6
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D RCST
= +
Tf
fi
Ti
fi
Spectrum a 25 oC
Spectrum a 26 oC
Spectrum a 90 oC
fi
Tf
Ti
Tf
Ti
Multivariate analysis. Fundamentals
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Multivariate analysis. Different approaches
‐ Multivariate analysis based on a physico‐chemical model
‐ Acid‐base equilibria stoichiometry and protonation constants
‐ Ligand:DNA interaction equilibria stoichiometry and binding constants
‐ Thermally‐induced unfolding of a folded structure thermodynamic variables (H0, S0, G)
‐ Multivariate analysis based without any model
‐ Soft‐modelling
‐ Example: MCR‐ALS (Romà Tauler, IDAEA)
‐ Hybrid approaches
‐ Anna De Juan (UB), Romà Tauler, …
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Proposed nature of the transition H0 (kcal/mol) S0
(cal/K·mol)Tm
(oC)Intramolecular i-motif partially unfolded strand 55 180 32
Partially unfolded strand completely unfolded strand 34 107
Spectroscopically‐monitored unfolding of an i‐motif
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220 230 240 250 260 270 280 290 300 310 320
-4
-2
0
2
4
6
8
10
12
Wavelength/nm
CD
/mde
g 20 40 60 804
6
8
10
12
Temperature/oC
CD
at 2
64nm
/mde
g
20 40 60 80 100-5
0
5
10
15
20x 10-7
Temperature/oC
Con
cent
ratio
n/M
220 240 260 280 300 320-3
-2
-1
0
1
2
3
4
5
6
7x 106
Wavelength/nm
Mol
ar e
lliptic
ity
__ Hybrid structure
__ Parallel structure
__ Unfolded strand
__ Baseline drift
Spectroscopically‐monitored unfolding of a G‐quadruplex
Gargallo, 2014
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Acid‐base equilibria of i‐motif structures
Benabou et al. 2014
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Acid‐base equilibria of i‐motif structures
Benabou et al. 2014
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TMPyP4: model ligand oftenused in interaction studies
Interaction of an i‐motif with a ligand
Hard‐modelling1:2 (DNA:ligand) stoichiometrylog binding constant = 12.4 ± 0.2
Khan et al. 2007
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250 300 350 400 450 500 550 600
-20
-10
0
10
20
30
40
50
60
Wavelength/nm
CD
/mde
g
0 1 2 3 40
0.2
0.4
0.6
0.8
1
1.2x 10-5
Con
cent
ratio
n/M
CCV/CDNA
300 400 500 600-1
-0.5
0
0.5
1
1.5x 107
Wavelength/nm
Mol
ar e
lliptic
ity
ckit21T21CV1:1 complex
Interaction of a parallel G‐quadruplex with a ligand
Hard‐modelling1:1 stoichiometrylog binding constant = 4.7 ± 0.2 Benito et al. In preparation
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Three melting experiments were monitored with
three different spectroscopic techniques (molecular
fluorescence, circular dichroism and molecular
absorption)
1. Melting of 5’-Fluorescein-
TAGGGTTAGGGT-Dabsyl-3’ (SG)
2. Melting of the 1:1 mixture of SG and
its complementary sequence
3. Melting of the 2:1 mixture of SG and
its complementary sequence
Melting of a G‐quadruplex and Watson‐Crick duplex
Jaumot et al. 2006
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Melting of a G‐quadruplex and Watson‐Crick duplex
1. Melting of 5’-
Fluorescein-
TAGGGTTAGGGT-
Dabsyl-3’ (SG)
2. Melting of the 1:1
mixture of SG and
its complementary
sequence
3. Melting of the 2:1
mixture of SG and
its complementary
sequence
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Jaumot et al. 2006
1. Melting of 5’-
Fluorescein-
TAGGGTTAGGGT-
Dabsyl-3’ (SG)
2. Melting of the 1:1
mixture of SG and
its complementary
sequence
3. Melting of the 2:1
mixture of SG and
its complementary
sequence
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Other example: analysis of DNA microarrays
www.genome.gov
NCI60 data set (Ross et al. Nat. Gen. 2000, 24, 227)
60 samples:◦ breast carcinoma (BR)◦ central nervous system tumor (CNS)◦ colon carcinoma (CO)◦ non‐small lung cancer (NSLC)◦ leukemia (LE)◦ melanoma (ME)◦ ovarian carcinoma (OV)◦ prostate cancer (PR) ◦ renal carcinoma (RE)
mRNA was extracted and hybridized to cDNA microarrays including 9703 human cDNA clones that represented approximately 8000 different genes.
The analyzed data set contains 60 samples and 1416 variables
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Component Cancer
a melanoma, breast
b melanoma, breast
c Not identified
d
e Central nervous system
f leukemia
g Colon carcinoma
Other example: analysis of DNA microarrays
Jaumot et al. 2006
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Other example: analysis of DNA microarrays
Jaumot et al. 2006
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AcknowledgmentsFormer PhD students: Dra. Montserrat Vives i Dr. Joaquim Jaumot
PhD Student: Sanae Benabou
Many MSc and Graduate Students
Collaborators: ◦ IQAC: Dr. Ramon Eritja, Dra. Anna Aviñó, Dr. Santi Grijalvo, Dra. Sonia Pérez‐Rentero, Dr. Rubén Ferreira …◦ IQFR “Rocasolano”: Dr. Carlos González◦ IBMB: Dr. Sébastien Lyonnais, Dra. Maria Solà …◦ Masaryk University (Brno, Czech Republic): Dr. Petr Taborsky and many MSc and PhD students◦ …
More at:◦ www.ub.edu/gesq/dna◦ [email protected]