Essential Basic Part Types

Coding Sequences (C) - Complete open reading frames (type I), or sequences encoding polypeptides but lacking either a stop codon (type II), a start codon (type III), or both (type IV)

Ribosome Binding Sites (RBS) - Sequence encoding a ribosome binding site, fused 5' to an ORF part

Terminators (TT) - Sequence causing transcription termination

(and more can come later, with their own part definitions and standards rules)

Potter Standard Polylinker

AGATCTPARTSEQUENCE1GGATCC AGATCTPARTSEQUENCE2GGATCC

GlySerAGATCTPARTSEQUENCE1GGATCTPARTSEQUENCE2GGATCC

GAATTCaaaAGATCTPARTSEQUENCE1GGATCCaaaCTCGAG

Type I Coding Sequences

AGATCTATG_MIDDLE_OF_PART_TAAGGATCC

AGATCTGTG_MIDDLE_OF_PART_TGAGGATCC

The start and stop codons are placed directly adjacent to the BglII and BamHI sites, respectively

Start codons are free to be ATG, CTG, TTG, or GTG

Coding Sequences

Type II AGATCTATGAAATTTCCCGGGAAATTTGGATCC

Type III AGATCTCATCATCATCATCATCATTAAGGATCC

Type IV AGATCTAAATTTCCCGGGAAATTTCCCGGATCC

Coding sequences allow the construction of ORF fusions for chimeric and tagged proteins. GlySer scars separate junctions between fused peptides.

Ribosome Binding Sites

AGATCTGAAAGAGGAGAAAGGATCC

The spacing of a ribosome binding site relative to the start codon is fixed. Shown is a (likely) strong RBS

AGATCTATG_ORF_Part_TAAGGATCC

.RBS.

AGATCTGAAAGAGGAGAAAGGATCTATG_ORF_Part_TAAGGATCC

Promoters

+1 |AGATCTTCC_Middle_of_Ptet_TAGAGATACTGAGCACGGATCC

The transcriptional start site (+1) is located at the position directly 5' to the BamHI site (whenever possible)

Terminators

...CUUUCUGCGUUUAUA3' |AGATCTCCA_Middle_of_Ptet_CTTTCTGCGTTTATAGGATCC

The transcriptional termination site is located at the position directly 5' to the BamHI site (whenever possible)