ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO...

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Transcript of ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO...

Page 1: ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN - COATED PLATES IN N2B MEDIUM AND WERE PASSAGED.
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ONE DAY IN THE LAB AT PRINCETON…ES CELLS WERE

MAINTAINED IN SERUM-FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN-COATED PLATES IN N2B MEDIUM AND WERE PASSAGED EVERY 2–4 DAYS. DISSOCIATED CELLS WERE HARVESTED IN N2B MEDIUM, PELLETED, RESUSPENDED IN N2B AND REPLATED DIRECTLY.

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HOW FAR DID ONE

GRADUATE STUDENT

HAVE TO GO TO

REPRODUCE ONE

EXPERIMENT?

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FROM NEW JERSEY, USA TO SCOTLAND. TOTAL COST FOR TWO MONTHS OF WORK, TRAVEL AND REAGENTS: $10,000 TO LEARN ONE EXPERIMENT.

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64% OF PUBLISHED STUDIES ARE NOT REPRODUCIBLE

SOURCE: BAYER. 2011 PROJECT TESTING 67 STUDIES PUBLISHED IN REPUTABLE SCIENCE JOURNALS.

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TRADITIONAL SCIENTIFIC ARTICLE:

POSITION THE METAPHASE SPINDLE AT 3 O’CLOCK AND HOLD IT WITH HOLDING PIPETTE. APPLY PIEZO PULSES TO PENETRATE THE ZONA PELLUCIDA. TOUCH THE METAPHASE PLATE WITH THE ENUCLEATION PIPETTE. ASPIRATE THE SPINDLE AND WITHDRAW THE PIPETTE.

• IS IT IDEAL FOR RESEARCH USE OR TEACHING?

• WHAT DOES IT SAY?

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WELCOME TO THE WORLD’S FIRST PEER REVIEWED SCIENTIFIC VIDEO JOURNAL

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THE QUALITY AND PRESTIGE OF THE MOST RESPECTED SCIENTIFIC JOURNALSENERGIZED WITH THE VISUAL POWER OF FULLY PRODUCED INSTRUCTIONAL VIDEO

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• THE SOLUTION FOR POOR EXPERIMENTAL REPRODUCIBILITY

• REDUCE TIME AND LABOR INTENSIVE NATURE OF LEARNING NEW EXPERIMENTAL TECHNIQUES

• EXTENSIVE PEER-REVIEWED VIDEO CONTENT FROM THE MOST VITAL AREAS OF SCIENTIFIC AND MEDICAL RESEARCH

A UNIQUE ONE-STOP RESOURCE

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JOVE IS A “MUST HAVE” RESOURCE AT TOP SCIENTIFIC INSTITUTIONS LIKE THESE:

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JOVE IS A “MUST HAVE” RESOURCE AT TOP SCIENTIFIC INSTITUTIONS LIKE THESE:

• STANFORD UNIVERSITY• NATIONAL INSTITUTES OF HEALTH• BOSTON UNIVERSITY• PRINCETON UNIVERSITY• TUFTS UNIVERSITY• YALE UNIVERSITY• SALK INSTITUTE• UNIVERSITY OF TORONTO

• UNIVERSITY OF CHICAGO• UCLA• DARTMOUTH COLLEGE• CORNELL UNIVERSITY• EMORY UNIVERSITY• WEIZMANN INSTITUTE• NORTHWESTERN UNIVERSITY• KING’S COLLEGE LONDON

PLUS HUNDREDS MORE…

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USAGE GROWTH

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SUBSCRIBER GROWTH

SUBSCRIBERS: UNIVERSITIES, COLLEGES AND PHARMA/BIOTECH COMPANIES

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CASE STUDY:

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CASE STUDY

• PURDUE UNIVERSITY

• WEST LAFAYETTE, INDIANA, USA

• ESTABLISHED MAY 6, 1869

• 40,000 STUDENTS

• 8,100 POSTGRADUATES

• 5 SCIENCE DEPARTMENTS

• 100+ RESEARCH LABORATORIES

• JOVE SUBSCRIBER SINCE 2010

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CASE STUDY

• DR. THERESA CASEY, ASSISTANT PROFESSOR IN THE DEPARTMENT OF ANIMAL SCIENCES AT PURDUE UNIVERSITY

• PH.D. FROM UNIVERSITY OF VERMONT

• PRACTICING RESEARCHER FOR 20 YEARS

• 19 PUBLISHED PAPERS IN PEER

• RESEARCH INTERESTS: MAMMARY DEVELOPMENT AND LACTATION

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CASE STUDYDR. CASEY’S PROBLEM: “HOW DO WE ISOLATE THE SUPRACHIASMATIC NUCLEI THROUGH SURGERY?”

• DR. CASEY DISCOVERED THAT ACTIVITY OF CIRCADIAN CLOCK GENES CHANGED DURING THE TRANSITION FROM PREGNANCY TO LACTATION IN OTHER TISSUES.

• CIRCADIAN CLOCK IS GOVERNED BY THE SUPRACHIASMATIC NUCLEUS (SCN) IN THE HYPOTHALAMUS AREA OF THE BRAIN.

• NEED TO LEARN TO DISSECT THE SCN BRAIN AREA USING SURGERY.

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CASE STUDYDR. CASEY’S SOLUTION: UTILIZE A JOVE VIDEO ARTICLE AS A PRODUCTIVITY TOOL

SLICE PREPARATION, ORGANOTYPIC TISSUE CULTURING AND LUCIFERASE RECORDING OF CLOCK GENE ACTIVITY IN THE SUPRACHIASMATIC NUCLEUS. SAVELYEV ET AL, 2011, JOVE (KAROLINSKA INSTITUTE)

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CASE STUDYTHE RESULTS: USING JOVE SAVES RESEARCHERS AND UNIVERSITIES BOTH MONEY AND TIME.

FOR RESEARCHERS:

• DR. CASEY SPENT TWO WEEKS TO LEARN THE EXPERIMENT. WITHOUT JOVE IT WOULD HAVE TAKEN HER TWO MONTHS.

• SAVED SIX WEEKS OF WORK TIME AND $15,000 IN RESEARCH FUNDS ($7,700 REAGENTS, $1,100 TRAVEL AND $6,200 SALARY)

FOR UNIVERSITIES:

• IF ONLY 10% OF THE 100+ LABORATORIES AT PURDUE COULD BENEFIT FROM A SUBSCRIPTION TO JOVE, SAVINGS OF $150,000 OR MORE COULD BE REALIZED.

• ROI = A MINIMUM OF 500%

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CASE STUDY

“I’ve been doing research for 20 years,

and having JoVE makes things so much

easier. You can educate yourself on

research other scientists are doing

around you and get familiarized on a

technique before you try it.”

- Dr. Theresa Casey

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WHAT DO OTHER

SUBSCRIBERS THINK

ABOUT JOVE?

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“AN ESSENTIAL RESOURCE”

“INDISPENSABLE”

“EXCELLENT”

“A TIME SAVER”

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“HIGHLY RECOMMENDED”

“VALUABLE”“INCREDIBLY USEFUL”

“I LOVE JOVE”

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Circulating Tumor Cells Were Associated with the Number of ...

Circulating Tumor Cells Were Associated with the Number of ...

750 rhLaminin-521 Geltrex rhVTN-N · CytoTune™-iPS 2.0 Sendai Reprogramming Kit workflow. • (B-C) At day 7, transduced HDFns were passaged onto rhLaminin-521, rhVTN-N at 0.5 2μg/cm

750 rhLaminin-521 Geltrex rhVTN-N · CytoTune™-iPS 2.0 Sendai Reprogramming Kit workflow. • (B-C) At day 7, transduced HDFns were passaged onto rhLaminin-521, rhVTN-N at 0.5 2μg/cm

Research Paper Cortistatin protects against intervertebral ... · 7.2. The culture medium was replaced every 3 days, and the cells were passaged when they reached 80-90% confluence.

Research Paper Cortistatin protects against intervertebral ... · 7.2. The culture medium was replaced every 3 days, and the cells were passaged when they reached 80-90% confluence.

University of Groningen Adipose derived stromal cells in ... · HL-1 CM were seeded at 10,000 cells/cm2 in 5% FBS Claycomb medium, afterwards cells were incubated with control 10

University of Groningen Adipose derived stromal cells in ... · HL-1 CM were seeded at 10,000 cells/cm2 in 5% FBS Claycomb medium, afterwards cells were incubated with control 10

A Tour of the Cell How cells were first discovered Cells were first observed using microscopes Today there are many ways to view cells and the structures.

A Tour of the Cell How cells were first discovered Cells were first observed using microscopes Today there are many ways to view cells and the structures.

NeurobiologyofDisease RegulatorofG … · 2008-08-28 · cally retained CD11b-positive cells were eluted as the positively selected cell fraction. Cells were incubated with FITC conjugated

NeurobiologyofDisease RegulatorofG … · 2008-08-28 · cally retained CD11b-positive cells were eluted as the positively selected cell fraction. Cells were incubated with FITC conjugated

Dendritic Cells Interactions with the Immune System ... · Dendritic cells (DCs) were firstly described by Ralph Steinman and Zanvil Cohn in 1973 [1]. These cells were identified

Dendritic Cells Interactions with the Immune System ... · Dendritic cells (DCs) were firstly described by Ralph Steinman and Zanvil Cohn in 1973 [1]. These cells were identified

Generation of Immunostimulatory Dendritic Cells …...cells were not activated through the selection procedure. Purity of CD344 cells depended on the source of the HPC. CD34@ cells

Generation of Immunostimulatory Dendritic Cells …...cells were not activated through the selection procedure. Purity of CD344 cells depended on the source of the HPC. CD34@ cells

Cell Structure Chapter 4. Cells Cells were discovered in 1665 by Robert Hooke Early studies of cells were conducted by ◦ Mathias Schleiden (1838) ◦ Theodor.

Cell Structure Chapter 4. Cells Cells were discovered in 1665 by Robert Hooke Early studies of cells were conducted by ◦ Mathias Schleiden (1838) ◦ Theodor.

THE OF BIOLOGICAL CHEMISTRY VOl. 266, No. 23, Issue 15, PP ... · the calcium phosphate technique. After 3 days, the cells were passaged and selected using a-minimal essential medium

THE OF BIOLOGICAL CHEMISTRY VOl. 266, No. 23, Issue 15, PP ... · the calcium phosphate technique. After 3 days, the cells were passaged and selected using a-minimal essential medium

Delivery macromoleculesinto living cells: Amethodthat ... · cultured plant cells (37). ... electrophoresis chemicals were pur-chased from Bio-Rad; tissue culture products were pur-

Delivery macromoleculesinto living cells: Amethodthat ... · cultured plant cells (37). ... electrophoresis chemicals were pur-chased from Bio-Rad; tissue culture products were pur-

Normal cellular counterparts of B cell chronic lymphocytic ......tion were enriched for B cells by E rosetting to deplete T cells. Cells were either used fresh or cryopreserved in

Normal cellular counterparts of B cell chronic lymphocytic ......tion were enriched for B cells by E rosetting to deplete T cells. Cells were either used fresh or cryopreserved in

Cell Structure Chapter 4. 2 Cell Theory Cells were discovered in _______________ __________________. Early studies of cells were conducted by - Mathias.

Cell Structure Chapter 4. 2 Cell Theory Cells were discovered in _______________ __________________. Early studies of cells were conducted by - Mathias.

0DWHULDO (6, IRU&KHP&RPP 7KLV Supplementary Information · Cells were plated overnight on 96-well plates at 8000 cells per well in growth medium. After seeding, cells were treated

0DWHULDO (6, IRU&KHP&RPP 7KLV Supplementary Information · Cells were plated overnight on 96-well plates at 8000 cells per well in growth medium. After seeding, cells were treated

Analysis of the Growth Pattern of Vero Cells Cultured on ... · Vero cells, a fibroblast lineage were used. These cells were cultured in Ham F-10 medium with 10% fetal calf serum

Analysis of the Growth Pattern of Vero Cells Cultured on ... · Vero cells, a fibroblast lineage were used. These cells were cultured in Ham F-10 medium with 10% fetal calf serum

 · Web viewThe HeLa cells were seeded in the 12-well plates at a density of 1 × 105 cells per well. After 20-h culture, the cells were treated with various endocytosis inhibitors

 · Web viewThe HeLa cells were seeded in the 12-well plates at a density of 1 × 105 cells per well. After 20-h culture, the cells were treated with various endocytosis inhibitors

TLR4-dependent activation of dendritic cells by an … · TLR4-dependent activation of dendritic cells by ... Peripheral blood mononuclear cells were isolated from ... Red blood cells

TLR4-dependent activation of dendritic cells by an … · TLR4-dependent activation of dendritic cells by ... Peripheral blood mononuclear cells were isolated from ... Red blood cells

How were cells discovered? The microscope led to the discovery of cells.

How were cells discovered? The microscope led to the discovery of cells.

Reappearance of Effector T Cells Predicts …...2020/05/11  · For analysis of activation markers, cells were analyzed in a lyse-wash fashion. Stained cells were acquired on BD FACS

Reappearance of Effector T Cells Predicts …...2020/05/11  · For analysis of activation markers, cells were analyzed in a lyse-wash fashion. Stained cells were acquired on BD FACS

downloads.hindawi.comdownloads.hindawi.com/journals/ecam/2020/3468791.f1.docx · Web viewCD8+ T cells were red round cells，CD4+ T cells were green round cells and NK cells were

downloads.hindawi.comdownloads.hindawi.com/journals/ecam/2020/3468791.f1.docx · Web viewCD8+ T cells were red round cells，CD4+ T cells were green round cells and NK cells were