ERT 320 Bio-Separation Engineering Semester 2 2012/2013 Huzairy Hassan School of Bioprocess...
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Transcript of ERT 320 Bio-Separation Engineering Semester 2 2012/2013 Huzairy Hassan School of Bioprocess...
ERT 320Bio-
Separation Engineering
Semester 2 2012/2013Huzairy Hassan
School of Bioprocess EngineeringUniMAP
Integrated Bio-Separation Schemes
“Ability to propose bio-separation techniques/processes and RIPP
(Recovery, Isolation, Purification and Polishing) scheme”
Integrated Bio-Separation Schemes
BIOPROCESS DESIGNINTEGRATED BIO-SEPARATION
PROCESSES
- Given a product and a desired annual production rate (plant throughput), bioprocess design endeavors to answer the following questions:What are required
amounts of raw materials and
utilities? What is total capital
investment?
What is the required size of process equipment
and supporting utilities?
Can the product be produced in an existing
facility or new plant required?
What is the manufacturing
cost?
What is the optimum batch size?
How long does a single batch take?
BIOPROCESS DESIGNINTEGRATED BIO-SEPARATION
PROCESSESHow much
product can be generated per
year?
How much???
What is the demand for various resources (raw mat, labor, utilities)?
What if ???What is ???
What is the environmental impact
of the process?
BIOPROCESS DESIGNINTEGRATED BIO-SEPARATION
PROCESSES- Process design is the conceptual work done prior
to building, expanding, or retrofitting a process plant.
- It consists of 2 main activities:Process synthesis
Process analysis
Is the selection and arrangement of a set of unit operations (process steps) capable of
producing the desired product at an acceptable cost and quality.
Is the evaluation and comparison of different
process synthesis solutions
BIOPROCESS DESIGN
SYNTHESIS OF BIO-SEPARATION PROCESS
BIOPROCESS DESIGNSYNTHESIS OF BIO-SEPARATION PROCESS
Rules of thumb (heuristics):(1) Remove the most plentiful impurities first
(2) Remove the easiest-to-remove impurities first
(3) Make the most difficult and expensive separations last
(4) Select processes that make use of the greatest differences in the properties of the product and its impurities
(5) Select and sequence processes that exploit different separation driving forces.
Primary Recovery Stages
Intracellular Products
Extracellular Products
Cell Harvesting (1)Centrifugation and membrane
filtration
Cell DisruptionHomogenizers
or bead mills
Removal of Cell DebrisCentrifugation,
microfiltration, extraction, etc
Product Extraction / Adsorption
Biomass Removal (2)Rotary vacuum
filtration, centrifugation,
membrane filtration
Intermediate Recovery Stages
Product Concentration
(1) & (2)
Product Renaturation
Ultrafiltration Reverse Osmosis
PrecipitationEvaporation
For solubilization of products (protein) in
inclusion bodies
Distillation
Final Purification Stages
Chromatography (3)
Crystallization & Fractional Precipitation
Dehydration & Solvent Removal
BIOPROCESS DESIGN
PROCESS ANALYSIS
Process / Biochemical Process Simulator
SuperPro Designer
BIOPROCESS DESIGN
PROCESS ECONOMICS
Capital Cost EstimationDirect fixed capital (DFC)
Working capitalStart-up & Validation costs
Operating Cost Estimation Raw materials, labor,
consumables, Lab/QC/QA, Waste treatment & Disposal, Utilities, Equipment-dependent Costs,
Miscellaneous
Profitability Analysis Gross margin, ROI,
Payback time
Production Plant
1) Citric Acid Production
Production Plant
2) Human Insulin Production
Production Plant
3) Therapeutic Monoclonal Antibody Production
ANY QUESTIONS
THANK YOU