Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural...
-
Upload
clement-farmer -
Category
Documents
-
view
214 -
download
1
Transcript of Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural...
![Page 1: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/1.jpg)
Epidemiological studies
![Page 2: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/2.jpg)
• Experimental studies • Scientifically rigorous• Considered as natural
experiments • Costly• Sometimes not feasible• Enrollment issues • Ethical issues
• Observational studies • Investigators is an
disinterested observer• People expose
themselves to the agent
• Ethical issues mitigated
![Page 3: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/3.jpg)
Observational study - Two types
• Cohort study• Cross-sectional study
![Page 4: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/4.jpg)
Analytical Studies
Introduction:
Analytical studies are either: • Observational Case-Control Cohort Study
• Experimental (Intervention): Animal Experiments
Human Therapeutic trials Preventive trials
![Page 5: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/5.jpg)
Analytic Studies
• Analytic studies, etiologic studies, are performed to test specific hypothesis about a specific health problem.
• In general, associations observed in descriptive studies are often the basis for gathering more specific data and testing hypothesis in additional studies.
![Page 6: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/6.jpg)
• Analytic studies involve the selection and comparison of two or more groups of persons, based on either their exposure or disease status…. WHY?
• To evaluate an association between exposure and disease.
• Analytic studies focuses on the magnitude of the association between the exposure and the health problem under the study.
![Page 7: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/7.jpg)
Study Design
•Life’s a journey. . .•We each carry the burden of personal and group risk factors and exposures.•As health professionals, we hope to identify those characteristics causing disease.
![Page 8: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/8.jpg)
Study Design
•Life’s a journey. . .•In individuals, the only way to know if a risk factor caused disease would be to find an exact double, living in a parallel universe, identical in every way to the exposed subject--except for the exposure.
![Page 9: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/9.jpg)
Study Design
•Life’s a journey. . .•If only the exposed subject developed disease, we could be certain the exposure was causal.
![Page 10: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/10.jpg)
Study Design
•Life’s a journey. . .•This is called the “counterfactual argument” because exact doubles and parallel universes do not exist.•(Stephen Hawking’s opinions notwithstanding. . .)•How to address this problem?
![Page 11: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/11.jpg)
Study Design
•Life’s a journey. . .•The best we can do is compare populations that are similar (not identical) in everything except the risk factor. •If we see increased disease only in the group with the risk factor, we can suspect that the risk factor caused the disease.
![Page 12: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/12.jpg)
Study Design
•Life’s a journey. . .•The “study base” is a population of individuals, each carrying the burden of personal and group risk factors.
•(Rothman and Greenland, Modern Epidemiology, 1998)
![Page 13: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/13.jpg)
Cohort Study
![Page 14: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/14.jpg)
![Page 15: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/15.jpg)
What is a cohort?
• From Latin word Cohors, group of soldiers– 6 centuries (100 men) form a cohort, – 10 cohorts form a legion (therefore 6,000 men).
• A century, then, would correspond to a company, a cohort to a battalion, and a legion to a regiment.
– Once a cohort was formed, no new soldier were added. Soldiers remain in the same cohort for rest of service.
– Attrition occurred mainly through death
![Page 16: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/16.jpg)
What is a cohort in epidemiology?
• A group of individuals/persons who are followed / traced over a period of time – Sharing a common characteristic or experience
• Some alternate names– Cohort study– Longitudinal study– Incidence study – Longitudinal study
![Page 17: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/17.jpg)
![Page 18: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/18.jpg)
Retrospective (Case-Control)
a b
dc
DISEASEpresent absentEXPOSURE
present
absent
cases
controls
Total
Total
Pro
sp
ecti
ve
(C
oh
ort
)
exposed
Not exposed
A fourfold table
Mausner, 1985
![Page 19: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/19.jpg)
• The difference between the two types of studies lies in the way the study groups are assembled
• With either method of study, if there is a positive association between the factor and the disease:
• Those exposed will tend to develop the disease (group a),
• Those not exposed will tend not to develop it (group d).
![Page 20: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/20.jpg)
The Prospective Approach
• The general concept of a prospective study is relatively simple.
• This type of study has been described by a variety of items: -Cohort
-Incidence-Longitudinal-Forward looking-Follow-up
![Page 21: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/21.jpg)
Prospective Cohort “concurrent”• COHORT Study Retrospective Cohort “non-concurrent” Historical prospective
![Page 22: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/22.jpg)
The Prospective Approach (cont.)
• 1. It starts with a group of people (a cohort) all considered to be free of a given disease.
• Information is obtained to determine persons having a particular characteristic (certain exposure) that is suspected of being related to the development of disease being investigated.
• 2. These individuals are then followed for a period of time to observe who develops/or dies from that disease
• 3. Incidence or death rates for the disease are then calculated.
![Page 23: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/23.jpg)
The Prospective Approach (cont.)
• 4. Rates are compared for those with the characteristic and those without it.
• 5. If the rates (of development of disease) are different, an association can be said to exist between the characteristic (exposure) and the disease.
• 6. It is important to obtain information on other characteristic of the study groups: age, sex, … to account for an influence of any factors related to the disease.
![Page 24: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/24.jpg)
Study Design
Exposure(Risk Factor)
Disease (Outcome)
+
+
_
_
![Page 25: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/25.jpg)
Cohort Studies
• Begin with sample “Healthy Cohort” (i.e., subjects without the outcome yet)
• Start with Exposure status, then compare subsequent disease experience in exposed vs. unexposed.
![Page 26: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/26.jpg)
Study Design
Exposure(Risk Factor)
Disease (Outcome)
+
+
_
_
![Page 27: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/27.jpg)
Case-Control Studies
• Begin with sample of “Cases and Controls”
• Start with Disease status, then assess and compare Exposures in cases vs. controls.
![Page 28: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/28.jpg)
Study Design
Exposure(Risk Factor)
Disease (Outcome)
+
+
_
_
![Page 29: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/29.jpg)
Cross-Sectional Studies
• Begin with “Cross-sectional” sample
• Determine Exposure and Disease at same time
![Page 30: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/30.jpg)
What is a cohort ?• A cohort is a group of persons who share a
common experience within a defined time period.
Example: • Birth cohort, marriage cohort, occupational
cohort
![Page 31: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/31.jpg)
Cohort Study (cont.)
Essential points:• Exposed individuals in the study should be
representative of all exposed persons.
• Unexposed persons should be representative of all unexposed persons in the population.
![Page 32: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/32.jpg)
Cohort Study (cont.)
Selection of Cohorts: several approaches• Accessible group (volunteers)• Group with available records/history of
exposure• Group experiencing some particular exposure
(arising during work)
Cohorts may be heterogeneous or homogenous
![Page 33: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/33.jpg)
• Heterogeneous: with respect to some previous exposure as study of lung cancer and smoking.
• Homogenous in exposure:As study of the frequency of cancer among
asbestos workers. The comparison group is the general population
valuesDemonstrate excess in deaths among asbestos
workers.
![Page 34: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/34.jpg)
Cohort studies
Intuitive approach to studying disease incidence and risk factors:
1. Start with a population at risk2. Measure characteristics at baseline3. Follow-up the population over time with
a) surveillance or b) re-examination4. Compare event rates in people with and
without characteristics of interest
![Page 35: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/35.jpg)
Cohort studiesCan be large or smallCan be long or shortCan be simple or elaborateCan be local or multinationalFor rare outcomes need many people and/or
lengthy follow-upMay have to decide what characteristics to
measure long in advance
![Page 36: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/36.jpg)
Community surveillance enhances generalizability of cohort findings
1. Cohort Community: compare incidence rates and characteristics of events in residents who do and who do not participate in cohort
2. Communities Cohort: compare the study community CHD experience with areas in the U.S.
![Page 37: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/37.jpg)
COHORT STUDIES
• Cohort Study
– Key Point:–Presence or absence of risk factor is
determined before outcome occurs.
![Page 38: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/38.jpg)
Cohort Studies
• Characteristics: follow-up period (prospective; retrospective)
• Merits: no temporal ambiguity; several outcomes could be studied at the same time; suitable for incidence estimation
• Limitations (of prospective type): expensive; time-consuming; inefficient for rare diseases; may not be feasible
• Effect measure: Risk Ratio (Relative Risk)
![Page 39: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/39.jpg)
Coho
rt D
esig
n
time
Study begins here
Studypopulation
free ofdisease
Factorpresent
Factorabsent
disease
no disease
disease
no disease
presentfuture
![Page 40: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/40.jpg)
Timing of cohort study
• Prospective cohort study • Retrospective cohort study • Ambidirectional cohort study
![Page 41: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/41.jpg)
Timing of cohort study
Prospective Cohort Study Exposure
Disease
![Page 42: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/42.jpg)
COHORT STUDIES
50% 50%
10% 90%R.F.
(+)
(-)
(+) (-)Disease Basic Idea:
See if those with the risk factor develop more disease than those without the risk factor
![Page 43: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/43.jpg)
COHORT STUDIES
• Basic Approach: Cohort Study– Identify Cohort (s)– Measure exposure and outcome variables– Follow for development of outcomes
![Page 44: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/44.jpg)
COHORT STUDIES
• Fixed Cohort
Exposure
(+)
(-)
x
x
x
X = outcome
Relative risk = (2/3)/(1/3) = 2.0
![Page 45: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/45.jpg)
30 a 70 b
3 c 57 d
Salad
(+)
(-)
(+) (-)Disease = Hepatitis A
a + b 100
c + d 60
Risk =a/(a+b) =0.3
Risk =c/(c+d) = 0.05
Rel. risk=
COHORT STUDIES
aa + b
cc + d
= 0.3/0.05 =6
Fixed cohort
![Page 46: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/46.jpg)
30 a 70 b
3 c 57 d
Salad
(+)
(-)
(+) (-)
Disease = Hep A
a + b = 100
c + d = 60
Odds Ratio: (a/c)/(b/d)=(a/b)/(c/d)
Rel. risk=
COHORT STUDIES
aa + b
cc + d
= 0.3/0.05 =6
(30/3)/(70/57)= 8.14
![Page 47: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/47.jpg)
COHORT STUDIES•Dynamic Cohort
Exposure
(+)
(-)
Rel. Risk
= 2/3/2/3 =1
or
2/5py/2/10py
= 2.0Years
XX
XX
![Page 48: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/48.jpg)
COHORT STUDIES
• Cohort : 16, 936 Harvard grads
• Measure: Question re: activity level
• Follow: “Sedentary”: 24 CHD deaths per 10,000 person-years
• vs. “Active”: 16 CHD deaths per 10,000 person-years
• Relative risk = 24/16 = 1.5
![Page 49: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/49.jpg)
COHORT STUDIES
• Questions:
• Findings due to confounding?
• Could subclinical disease have affected the risk factor (activity)?
![Page 50: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/50.jpg)
COHORT STUDIES
• Take-Home Message:• The best measure of effect is the “relative risk.”
For a fixed cohort, this will be the ratio of the cumulative incidences. For a dynamic cohort, this will be the ratio of the incidence rates.
• The odds ratio can be used for fixed cohorts comparing cumulative incidences. It will be close to the relative risk for rare diseases.
![Page 51: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/51.jpg)
COHORT STUDIES
• Variations on a theme:
• Retrospective (Historical) Cohort
![Page 52: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/52.jpg)
COHORT STUDIES
• Prospective: Outcomes have not yet occurred as study begins. Example: Women’s Health Study.
• Retrospective: Outcomes have already occurred as the study begins. Example: finding a trove of medical records allowing you to follow a cohort born in 1880 to death.
![Page 53: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/53.jpg)
COHORT STUDIES
• Utility and Strengths
• Incidence and natural history
• Temporal sequence
• Avoid survivor bias
• Avoid reporting bias
• Look at multiple outcomes
![Page 54: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/54.jpg)
Purpose of cohort study
• Descriptive – To describe the incidence of certain outcome
overtime• Analytical
– To analyze association between risk factors and outcomes
![Page 55: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/55.jpg)
Two basic types of cohort study
• Prospective cohort • Retrospective cohort
![Page 56: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/56.jpg)
Types of population studied
• 3 types of population – Open or dynamic– Fixed – Closed
Type Defined by Follow up Measure of disease
Open or dynamic Changeable characteristic
Members come and go, losses can occur
Incidence rate
Fixed Irrevocable event No gain in member, loses may occur
Incidence rate
Closed Irrevocable event No gain in member, no loses occur
Cumulative incidence
![Page 57: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/57.jpg)
Open/ dynamic population
• Membership criteria is changeable – Smoking– Alcohol drinking – Certain occupation – People living in a geographic area
• Consider / account for in- and out- migration
![Page 58: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/58.jpg)
Examples: Open/ dynamicpopulation
• Cancer incidence among never-married men aged 15-54 yrs and resided in San Francisco – Period of FU 1973-1990– Changeable characteristics- marriage, age, place
of residence – FU of 1,930,000 person years – incidence of NHL increased by 20 times, but rectal
cancer remained same
![Page 59: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/59.jpg)
Fixed population
• Membership criteria is irrevocable / fixed – Giving birth to a baby – Undergoing a medical procedure – Eating contaminated food– Joining military – Presence during a disaster
• Exposures do not change over time • Followed for a fixed period• Loss of members may occur
![Page 60: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/60.jpg)
Examples: Fixed population
• World war II- Hiroshima & Nagasaki nuclear bombing – Biological effects of radiation exposure – Monitoring of mortality and cancer incidence
among 94000 residents who were in the city and 27000 residents who were outside the city during bombing
– Follow up for disease incidence
![Page 61: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/61.jpg)
Closed population
• Same as fixed cohort – Membership criteria is irrevocable / fixed
• Giving birth to a baby • Undergoing a medical procedure • Eating contaminated food• Joining military • Presence during a disaster
– Exposures do not change over time – Followed for a fixed period
• But NO Loss of members occur
![Page 62: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/62.jpg)
Examples: Closed population
• To examine risk of GI infection during one week after an eating of certain type of food among people attending a party – Assuming the symptoms would start relatively fast and
will end by 7 days – Everybody who attended the party is a member – Follow up starts at the end of the party and continues
till one week– No loss of member, as period of observation is short – Estimate cumulative incidence
![Page 63: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/63.jpg)
Exposure
• Event that has bearing on the outcome of interest
![Page 64: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/64.jpg)
Cohort Study (Prospective Design)
Passive smoking & respiratory infections in children
• Is passive exposure to tobacco smoke associated with increased respiratory infections in children ?
• Design:Children exposed and not exposed tobacco
smoke in their homesFollow them in time for disease occurrence.
![Page 65: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/65.jpg)
Children (<12 yrs)
1000
Family smoker500 childrenExposed
Family non-smoker500 childrenNot exposed
1 year
Diseased 300
Not diseased 200
Diseased 120
Not diseased 380
OutcomeStart
![Page 66: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/66.jpg)
Rate: Incidence rate
•Incidence of Resp. Infection among exposed children: 300
500 = 60%
•Incidence of Resp. Infect. Among non exposed children: 120
500 = 24%
![Page 67: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/67.jpg)
Cohort Study (cont.)Relative Risk: Incidence rate among exposed Risk Ratio Incidence rate in non exposed.
60 24 = 2.5
Relative Risk is a direct measure of risk (to assess the etiologic role of a factor in disease occurrence).
300 x 500 500 120
![Page 68: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/68.jpg)
Cohort Study (cont.)Relative Risk:Smoking- Lung Cancer mortality: RR=18.57- Myocardial infarction mortality: RR=1.35
It measures the strength of association
![Page 69: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/69.jpg)
Cohort Study (cont.)Attributable Risk: The absolute difference in
Incidence rates among groups. “Risk Difference” RD
60 - 24 = 36%The extent to which the incidence of disease can
be attributed to the risk factor
Smoking-Lung cancer mortality: RD=1.23-Myocardial infarction mortality RD=1.25
![Page 70: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/70.jpg)
Annual Death Rates / 100,000 persons Exposure Category
Lung Cancer Coronary Heart D. 166 599
7 422
166 / 7 = 23.7 599 / 422 = 1.4
166 – 7 =159 599 – 422 = 177
Heavy smokersNonsmokers
Measures of Excess Risk
Relative Risk:
Attributable risk:
Doll and Hill study : Mortality of British doctors cited from Mausner, 1985
![Page 71: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/71.jpg)
• The previous table suggests that prevention of coronary heart disease would require alteration of other factors in addition to smoking.
• The population attributable risk: relates both relative risk and frequency of the factor in the population
• i.e. a large proportion of the deaths from lung cancer in the total population are due to smoking not only because of the high RR associated with smoking, but also bec large proportion of the pop that smoke.
![Page 72: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/72.jpg)
![Page 73: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/73.jpg)
![Page 74: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/74.jpg)
Examples from the literature
• Framingham Heart Study initiated in 1948 by US Public Health Services:
to study the relationship of a variety of factors to the subsequent development of heart disease
Group of persons30 – 62yrs
6,500Both sexes
20 years follow up
Information:S. cholest.levelBl.pressure , weightCig. Smoking
outcome
![Page 75: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/75.jpg)
Occupation Based Studies to study effect of exposures
•
•Benzene workers and Leukemia• Coke-oven workers and lung cancer•Asbestos workers and lung cancer•Radium dial painters and oral cancer
![Page 76: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/76.jpg)
Males Females(45-54y) (45-54)
Initial Serum Cholesterol Level
RR RR1.35 1.31.48 1.432.85 2.573.25 2.89
200 - < 220220 - < 240320 - < 340340 - < 970
There is an increasing risk of CHD with increasing initialSerum cholest. Levels in the 45-54 age group from a relativeRisk of 1.13-3.25 M, 1.13-2.89 F
![Page 77: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/77.jpg)
1/9/2007 Cohort studies 77
Relating risk factors to health outcomes – questions
• Is this health condition associated with this exposure?– Association not = causation but may reflect it
• How strongly are these two factors related?– Strong association more likely causal
• How much of a disease can be attributed to a causative factor?
![Page 78: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/78.jpg)
9/27/2004 Cohort studies 78
What is an association?
Factors are associated if:• the distribution of one factor is
different for different values of another.
• knowing the value of one factor gives information about the distribution of the other.
![Page 79: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/79.jpg)
9/20/2000 79
Example – oral contraceptives and CHD
OC No OC Total
CHD 30 20 50
No CHD 30 70 100
Total 60 90 150
![Page 80: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/80.jpg)
9/27/2004 80
Example – oral contraceptives and CHD (positive association)
OC No OC Total
CHD 30 20 50
No CHD 30 70 100
Total 60 90 15030% (30/100) of controls OC, overall
60% (30/50) of CHD
cases used OC
![Page 81: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/81.jpg)
9/27/2000 81
Example – oral contraceptives and breast cancer
OC No OC Total
Cancer 15 35 50
No cancer 30 70 100
Total 45 105 150
![Page 82: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/82.jpg)
9/27/2004 82
Example – oral contraceptives and breast cancer (no association)
OC No OC Total
Cancer 15 35 50
No cancer 30 70 100
Total 45 105 15030% (30/100) of noncases used OC
30% (15/50) of cases used
OC
![Page 83: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/83.jpg)
6/9/2002 Cohort studies 83
Measures of association
• Can compare incidences (rate or proportion), prevalences
• Look at differences (e.g., “incidence difference”) (retains units)
• Look at ratios (e.g., “incidence ratio”) (no units)
![Page 84: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/84.jpg)
2/21/2006 Cohort studies 84
Translating measures of association
If incidence ratio for runners / non-runners = 3.0:–“Incidence in runners was 3 times that in non-runners.”
–“Incidence in runners was 3 times as great as in non-runners.”
–“Incidence in runners was 200% greater than incidence in non-runners.” [(3.0 – 1.0) / 1.0 = 200%]
![Page 85: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/85.jpg)
10/1/2001 Cohort studies 85
Translating measures of association
“Incidence in runners was 3 times greater than incidence in non-runners” is ambiguous
• Does it mean incidence ratio = 3.0 ?
• Does it mean incidence ratio = 4.0 ?
![Page 86: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/86.jpg)
10/1/2001 Cohort studies 86
Translating measures of association
If incidence for runners / non-runners = 0.30:–“Incidence in runners was 0.30 times that in non-runners.”
–“Incidence in runners was 30% of that in non-runners.”
–“Incidence in runners was 70% lower [or “less”] than incidence in non-runners.” [(1.0 – 0.30) / 1.0 = 0.70 = 70%]
![Page 87: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/87.jpg)
10/1/2001 Cohort studies 87
Translating measures of association
Or, can say “Incidence in non-runners was 3.3 times as great as incidence in runners”.
![Page 88: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/88.jpg)
2/21/2006 Cohort studies 88
Measures of impactConcept of attributable risk
– How much of a disease can be attributed to a causative factor?
– What is the potential benefit from intervening to modify the factor?
Important for– Public health policy– Legal liability– Clinical/individual decisions
![Page 89: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/89.jpg)
10/1/2001 Cohort studies 89
Example questions–Now that I am 35 years old, my CHD risk
from taking oral contraceptives is twice as great as when I was 25. But how much more risk do I have due to taking the pill?
–How much of the risk of heterosexual transmission of HIV might be eliminated through eliminating bacterial sexually transmitted diseases?
![Page 90: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/90.jpg)
10/1/2001 Cohort studies 90
Example questions• How many cases of asthma are due to
ambient sulfur dioxide?• What proportion of motor vehicular deaths
can be prevented by mandatory seat belt use.
• What proportion of perinatal HIV transmission has been prevented through the use of prenatal, intrapartum, and neonatal zidovudine (AZT)?
![Page 91: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/91.jpg)
10/1/2001 Cohort studies 91
Simplifying assumptions1. “Exposure” either causes or prevents the
outcome, but not both (no two-edged swords)
2. “Exposed” and “unexposed” groups are alike in all other respects (no confounding)
3. No other causes “compete” with the exposure
![Page 92: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/92.jpg)
10/1/2001 Cohort studies 92
Several concepts
Concepts
• “Absolute” versus “relative”
• Exposed versus total population
• Disease caused, disease prevented
![Page 93: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/93.jpg)
10/1/2001 Cohort studies 93
Many terms, many meanings
E.g., “attributable risk” can mean:
–Risk difference
–Population attributable risk percent
–Concept of assessing impact
![Page 94: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/94.jpg)
10/1/2001 Cohort studies 94
“Absolute” perspectiveHow much risk?
– In exposed persons: risk difference (I1– I0)
– In the total population: (I1– I0) x exposure prevalence (P1)
How many cases? (I1– I0) x # of exposed persons (n1)
![Page 95: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/95.jpg)
10/1/2001 Cohort studies 95
Relative perspective
What proportion of the risk is attributable?(What proportion of cases could be eliminated?)
In exposed persons: (I1– I0) / I1 = (IR–1) / IR
(Relative strength of association)
In the population: (I – I0) / I
(Strength of association and prevalence)
![Page 96: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/96.jpg)
10/6/2009 Cohort studies 96
How much risk? What %?How many cases? What %?
E E Total
D 40 20 60
D 960 1,980 2,940
Total 1,000 2,000 3,000
I1 =___ I0 =___ I =___
![Page 97: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/97.jpg)
10/6/2009 Cohort studies 97
How much risk? What %?How many cases? What %?
E E Total
D 40 20 60
D 960 1,980 2,940
Total 1,000 2,000 3,000
I1 =0.04 I0 =0.01 I =0.02
![Page 98: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/98.jpg)
6/9/2002 Cohort studies 98
Attributable risk diagram
I1 I1 I1 – I0 =
"AttributableI0
I0 n0 I0 n1I0 risk"
n0 n1 p1= n1/n
Attributable cases
![Page 99: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/99.jpg)
6/9/2002 Cohort studies 99
Prevented fraction diagram
I0 I0
I1I0 n0 I0 n1
I1
n0 n1 p1= n1/n
Prevented cases
![Page 100: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/100.jpg)
Advantages of Cohort Study
• Correct classification of exposure before disease develops.
• Permits calculation of incidence rates thus, a direct measure of relative risk, and attributable risk.
• Many possible outcomes to the same exposure can be studied.
• No chick egg dilema• Accurate
![Page 101: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/101.jpg)
Disadvantages of Cohort Study
• Large number of people are needed (large scale).• Time consuming (follow up)• Losing people in follow up (Attrition)• Expensive• Status of subjects may be changed leading to
error in classification of exposure eg. Change in habit, occupation.
• Administrative problems: loss of staff, funding, high costs of the extensive record keeping
![Page 102: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/102.jpg)
Non concurrent studies Retrospective Cohort
• The period of observation starts from some date in the past.
• They usually involve specially exposed groups or industrial populations.
• Done by using company records of past & present employees:
• Information: - date of employment - date of departure - duration, degree of
exposure - status: living/dead
![Page 103: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/103.jpg)
Nested case control studies
![Page 104: Epidemiological studies. Experimental studies Scientifically rigorous Considered as natural experiments Costly Sometimes not feasible Enrollment issues.](https://reader038.fdocuments.in/reader038/viewer/2022110400/56649dc05503460f94ab4402/html5/thumbnails/104.jpg)