Enzymeszaydoon
Transcript of Enzymeszaydoon
ENZYMESENZYMES&&
Chemical Chemical DigestionDigestion
Chemical Digestion Basics
• Dehydration Synthesis vs. Hydrolysis• Anabolic – Dehydration Synthesis
– Require the removal of water to form bonds
• Catabolic – Hydrolytic Reactions– Require the addition of water to break bonds
• Carbohydrates – mono and disaccharides• Lipids (triglycerides) – glycerol + fatty acids• Proteins – amino acids
enzyme
substrate
productsenzyme
Terminology:•Enzyme•Active site•Substrate(s)•Product(s)•Reversible reactions
Factoids:
Enzymes are:• Proteins
Factoids:Enzymes:• Lower the activation
energy needed for a reaction to take place
AB
Molecule C
+
Factoids:Enzymes:• Are unchanged after catalyzing a reaction
Factoids:Enzymes:• Turnover numbers are very high• Examples:
– 1 molecule of acetylcholinesterase -> 25,000 molecules of acetylcholine in 1 second
– 1 molecule of catalase breaks down 40 million molecules of hydrogen peroxide in 1 second
Factoids:Enzymes:• Are specific in their
activity• Lock and key model
Factoids:
Enzymes:• Sometimes require a
nonprotein factor = cofactor (metallic ion) or coenzyme (vitamin)– Examples:
• Iron, zinc, copper, sodium, potassium, vitamin B
Factoids:
Enzymes:• are usually named
after the substrate on which they act
• have names ending in –ase ( with a few exceptions)
Examples: • Cellulase• Sucrase• Lipase• Lactase• Acetylcholinesterase
Factors Affecting Enzyme Activity
Temperature
pH
Pepsin – stomach enzymeTrypsin – duodenum enzyme
more H+
Concentrationof Enzyme or Substrate
Inhibitors• Competitive Inhibition• Noncompetitive
Inhibition
Enzyme Uses
Papaya and Papain
Pineapple and Bromelain
Lactose = milk sugar• Lactase – enzyme produced by
cells that line the small intestine• Symptoms• Solutions
– Avoid dairy products (calcium problem) and prepared foods containing lactose
– Determine problematic amounts of lactose or source (i.e. milk, ice cream, aged cheeses)
– Lactase additives: add to milk (lactose content reduced by 70% or buy lactose-reduced milk
Enzyme Dietary Supplements
Questions Concerning Dietary Enzyme Supplements
• Are there people who do not produce enough of certain enzymes?
• Could an increase in digestive enzymes increase the digestion of food?
• Can you increase the amount of digestive enzymes by taking oral dietary supplements?