ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

ENZYME ACTIVITY

How to interpret enzyme graphs

(NEXTSLIDE)

Reading Graphs

Which graph shows the amount of substrate going to zero faster?

Time (sec)

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Time (sec)

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So…

Nope, the amount of substrate gets to zero in less time in the OTHER graph.

Time (sec)

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Time (sec)

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So…

YES!! The amount of substrate gets to zero in less time in this graph.

Time (sec)

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Time (sec)

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So…

Which enzyme works FASTER?

Time (sec)

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Time (sec)

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So… Nope, not this one. The faster the enzyme

works, the faster the substrate will decrease.

Time (sec)

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Time (sec)

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So…

YES!! This enzyme works faster because the substrate decreases faster!!

Time (sec)

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Time (sec)

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What do you think can affect the speed of an enzyme?

TEMPERATURE

AMOUNT OF LIGHT

pH


RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme.

ANYTHING ELSE?

TEMPERATURE

AMOUNT OF LIGHT

pH


RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme.

TEMPERATURE

AMOUNT OF LIGHT

pH


RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme.

ANYTHING ELSE?

TEMPERATURE

AMOUNT OF LIGHT

pH


RIGHT!! Changing the pH of the reaction can ALSO affect the speed of an enzyme.

TEMPERATURE

AMOUNT OF LIGHT

pH


Sorry, most enzymes are NOT affected by the amount of light…

Pick again

TEMPERATURE

AMOUNT OF LIGHT

pH

Factors affecting enzyme speed

We can make a graph of enzyme speed at different temperatures.

Graph of ENZYME SPEED

Speed of enzyme Temperature (°C)

What should go on the X axis?


Speed of enzyme Temperature (°C)

Nope, the speed of the enzyme is what we are MEASURING,it should go on the Y axis.


RIGHT! Temperature should go on the X axis.

Temperature (°C)0 20 50 6010 4030

What should go on the Y axis?(HINT: This is what we areMEASURING)

Amount of substrate

Speed of enzyme


Temperature (°C)0 20 50 6010 4030

What should go on the Y axis?(HINT: This is what we areMEASURING)• We’re not measuring the

amount of substrate this time.

Amount of substrate

Speed of enzyme


Temperature (°C)0 20 50 6010 4030

RIGHT!!The speed of enzyme should go on the Y axis.

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REMEMBER!! The enzyme is working FASTERAs you go UP the Y axis.

SLOW

FAST

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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Amylase is found in saliva. Click on the red button that is approximately at body temperature

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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SLOW

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Amylase is found in saliva. 5°C is close to freezing.

TOO COLD!!

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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SLOW

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Amylase is found in saliva. 22°C is close to room temperature.

TOO COLD!!

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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SLOW

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Amylase is found in saliva. 55°C is 131°F.

TOO HOT!!

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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Amylase is found in saliva. 37°C is body temperature. RIGHT!! Amylase will work best

at about 37°C because it is normally found in your mouth

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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Amylase works slowlyAt very low temperatures

Because it is not closeTo body temperature

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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Amylase will work Faster as it getsCloser to bodyTemperature.

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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FAST Amylase will work fastest

at bodyTemperature.

The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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SLOW

FAST As the temperature gets too hot,Amylase doesn’t work as well.

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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SLOW

FAST Click on the OPTIMUM TEMPERATURE

Let’s GRAPH!!

Temperature (°C)0 20 50 6010 4030

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FAST Click on the OPTIMUM TEMPERATURE

RIGHT!!

What is the optimum temperature for this enzyme?

Temperature (°C)0 20 50 6010 4030

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10°C

20°C

30°C


Temperature (C)0 20 50 6010 4030

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10°C

20°C

30°C

REMEMBER: The OPTIMUM is the temperature the enzyme

works fastest at.


Temperature (°C)0 20 50 6010 4030

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10°C

20°C

30°C

REMEMBER: The OPTIMUM is the temperature the enzyme

works fastest at.

Right!!

What is the optimum pH for this enzyme?

pH0 4 10 122 86

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9

10

11

What is the optimum pH for this enzyme?

pH0 4 10 122 86

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9

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11

Look closely…

Right!!


Temperature (°C)20 40 70 8030 6050

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20°C

63°C

48°C

Right!!

What temperature does the enzyme work best at?

Time

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Temperature =

25°C

Time

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Temperature =

35°C

Time

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Temperature =

30°C

At 25°C At 30°C At 35°C

REMEMBER: the faster the substrate decreases, the faster the

enzyme is working

Time

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Temperature =

25°C

Time

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Temperature =

35°C

Time

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Temperature =

30°C

At 25°C At 30°C At 35°C

TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE

THIS SHORT QUIZ

QUIZ - QUESTION 1

Where in your body is the amylase enzyme found?

SALIVA STOMACHLIVER

Right!!

QUIZ - QUESTION 2

Click on the CATALYST in this reaction:

STARCH SUGAR

AMYLASE

QUIZ - QUESTION 2

REMEMBER: The catalyst performs the reaction, but isn’t used up

STARCH SUGAR

AMYLASE

Right!!

QUIZ - QUESTION 3 Click on the substrate that is most likely to

react with the enzyme below:

PROTEIN LIPIDSTARCH

QUIZ - QUESTION 3 REMEMBER: The subtrate that fits into a

protein will be the one that reacts with it.

PROTEIN LIPIDSTARCH

Right!!

PROTEIN

QUIZ - QUESTION 4

What is the most likely name of the enzyme in question 3 that reacts with protein?

PROTEINOSE

PROTEINASEPROTEINZYME

PROTEINATION

QUIZ - QUESTION 4

NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose)

PROTEINOSE


PROTEINATION

QUIZ - QUESTION 4

NOPE: think about “lactase” and “amylase”

PROTEINOSE


PROTEINATION

Right!!

QUIZ - QUESTION 5 Click on the part of the graph that shows

the OPTIMAL temperature for this enzyme

Temperature (°C)

Enz

yme

Act

ivity

QUIZ - QUESTION 5 REMEMBER: the OPTIMAL temperature

for an enzyme is the temperature where the enzyme works BEST (highest activity)

Temperature (°C)

Enz

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Act

ivity

Right!!

QUIZ - QUESTION 6 Click on the graph that shows the optimum

pH for this enzyme.

Time

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Time

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pH = 5.0 pH = 3.0pH = 7.0

QUIZ - QUESTION 6

REMEMBER: Substrate decreases in LESS time if the enzyme works faster

Time

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Time

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Time

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pH = 5.0 pH = 3.0pH = 7.0

Right!!

You have successfully completed the enzyme powerpoint.

ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

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