• BY:

• HOLLY ERIN WHITE

• JORGE GOITIA

• REEM ALAZHRI

• ZAYNAB YAHYA GORKI

ENZYMES

What are Enzymes?

• Enzymes are biological catalysts

made of protein.

What are Catalyst?• A catalyst is a substance, which can alter

or speed up a chemical reaction, without

itself being chemically changed at the

end of the reaction.

•  

What reaction do Enzymes Catalyze?

• Digestion is an enzyme-catalyzed process. Some food

molecules are large and insoluble in water; these molecules

cannot diffuse through the cell surface membrane. Imagine

a meal consisting of fish and rice:

• Rice contains starch, a very large carbohydrate molecule.

• Fish contain two types of large molecules: protein and fats.

The effect of Catalase on Hydrogen Peroxide.

•EXPERIMENT

INTRODUCTION!

• Catalase is an enzyme found in many plant and

animal tissues. The enzyme has the function of

breaking down dangerous peroxide ions. As it

does, oxygen gas is released –because the

reaction is rapid, the oxygen bubbles form froth

on the top of a reacting solution.

In the experiment the key factors that could affect the results o

f theExperiment will be:

• 1. The temperature of the surroundings and test

tubes

• 2. The pH value of its surroundings

• 3. The substrate volume

• 4. Mass of enzyme used

OBJECTIVES:

• To determine the effect of catalase on hydrogen peroxide.

• To conduct the experiment correctly.

• To gather appropriate data for analysis and conclusion.

MATERIALS:

• Hydrogen peroxide

• Test tubes

• Potatoes

• Egg white

• Yeast

• Wooden splint

• Graduated cylinder

• Knife

• Chopping board

• Beaker

• String spoon

PROCEUDRE:• Gather all the needing materials.

• Peal one potato and cut it in small pieces, get the beaker and pour the

cutting potatoes into, then boil it.

• Peal one potato again and cut it in small pieces.

• Break your egg white and pour it into the beaker

• Measure 10ml of hydrogen peroxide into each test tube or boiling tubes.

• Label each test tube A, B, C, D.

• To each test tube, add one of the following substances: A. some chopped

raw potato, B. egg white, C. some chopped boiled potato, D. some yeast.

• Place each test tube in the test tube keeper.

• Gently observed the experiment

• Record your observation, and explain them as fully as you can.

ANALYSIS AND INTERPRETATION OF DATA:

A. Raw potato: we think that the enzyme will react greater with the potato and produce

More froth when it is cut up.

This is because as you cut it up into smaller bits, you make the

Surface area greater meaning there are more enzymes to react with each

Other, and there will be more successful collisions between the two

Reactants.

 

B. Boiled egg white: when the egg was drop in hydrogen the ph value increase that is why it

produced a lot of bubble and the enzyme speed up faster.

C. Boiled potato: during the process of boiled potato there was no reaction or bubble this was due

to the lack of Enzymes that was already burn when boiling.

 

E. Yeast: This is always happening but at a very low rate so you don't notice it. If you add yeast to

hydrogen peroxide it will speed up the decomposition of H2O2. However the yeast does not react

with the H2O2 and nothing is chemically altered in the yeast. That is because yeast is a catalyst.

DATA:

TUBE CONTENT HIEHT OF FROTH IN ONE MINUTE(CM)

A RAW POTATOES 9.8CM

B BOILED EGG WHITE

1CM

C BOILED POTATOES NOTHING

D YEAST 9CM

OBSERVATION:

A. Raw Potatoes :the raw potatoes produced many bubble and the

enzymes speed up faster.

B. Boiled Egg White: The boiled egg white actually have bubble but

the speed of the enzymes was quite slow this is because of the PH

value .

C. Boiled Potatoes: The boiled egg has not reaction or produced

anything this is because the enzymes are already broken down.

D. Yeast: The yeast reaction to the hydrogen peroxide has a greater

decomposition but a very low rate. Many bubble present.

CONCLUSION:• With todays experiment we quite learn and observed

how enzymes factors affect different substances. And

we do hope that this lesson will never skip out of our

memories.