Key:

TD → Triple- decarb. EnterobacteriaceaeIMViC PYR Urease Lysine Ornithine TD Res to: Notes

E. coli +,+,=,= = +

Klebsiella =,=,+,+ + = B-lactamase, cephalosporin

-Encapsulated-ONPG + - K. oxytoca → Indole +

Pantoea =,=,+,+ =(Differs

from Kleb)

Inducible Res to B-lactamase

- Encapsulated-Res is induced by 2nd & 3rd gen. cephalosporins & Fluoroquinolones

Enterobacter Sp.

=,=,+,+ -Encapsulated

Citrobacter V,+,=,+ = -Differed from E. coli by use of citrate

Serratia =,V,V,+ -S. marcescens → VP +

Salmonella =,+,=,V = + -S. typhi → ODC =

Shigella V,+,=,= = = -The most Biochemically Nonreactive - S. sonnei → ONPG & ODC +- S. dysenteriae → only Cat =

Proteus V,+,=,= + -P. vulgaris → indole + - P. mirabilis → indole =

Morganella +,+,=,= + -PMP in pink and green suit (urease & PAD +)

Providencia +,+,=,+ +

Yersinia =,+,=,= = -GS → Safety pin-Motile at 22°C

Y. pestis → Nonmotile-Y. pseudo tuberculosis → Bile-esculin +

Y. enterocolitica → VAll are:

1. Ox = 2. Catalase & Nitrate + 3. Motile 4. Facultative anaerobes 5. Non-spore a. Except for “SKY”

6. Glucose fermenters i. Shigella, Klebsiella & Yersinia

Enterobacteriaceae TSI & KIA

Mac Hek XLD Bap Specialmedia

Notes

E. coli A/Ag LF Y Y Hem - Most common cause of the UTI - Mac sorb → 0157 → clear

Klebsiella A/Ag LF(Mucoid)

Y Y -Abundant gas

Pantoea A/Ag LF(Mucoid)

Y Y Smooth, grey -Abundant gas- P. agglomerans →Bap → Lemon-Y

Citrobacter A/Ag,H₂S

LF(late)

C(w or w/o Bc)

R, Y or C

-Resemble E. coli-C. freundii → most common isolate

Serratia A/A LF(late)

C Y or C -S. marcescens → Most common isolate & the #1 cause of nosocomial infec in nurseries-Mac after 48hrs → red pig. Or red centers

Salmonella K/Ag,H₂S

NLF G/BL with Bc

R with Bc

-GM broth-SS agar

-Abundant H₂S -S. typhi → Little to No H₂S or gas

Shigella K/A NLF G C -Mac → LF after several days

Proteus K/A,H₂S

NLF C (w or w/o H₂S)

C Swarming -TSI → W or w/o gas-P. vulgaris → swarms the most-P. mirabilis → more commonly isolated

Morganella K/A NLF C R, C -PMP → Nosocomial infecs

Providencia K/A NLF C Y, C

Yersinia K/Ag NLF Salmon Pink

Y, C -CIN -TSI → usually w/o gas- Fried egg appearance as it ages -CIN → Target colonies -Aeromonas also grows on CIN, but is OX +

Media

Species Serotype A.k.a Disease NotesE. coli STEC / EHEC Shiga toxin .0157:H7 -The most ID’d diarrhea Agent in North America

Key:

C → Clear/Colorless Y → Yellow R → Red G → Green BL → Blue Bc → Black Center

producing -Non-motile-Mac sorbitol → Clear

ETECE. toxigenic -childhood diarrhea in other countries

(bacterial cause)-traveler’s diarrhea

-Giardia is the parasite that causes it

EPEC E. pathogenic - Daycare center / nursery outbreaks

EIEC E. Invasive - Watery / bloody diarrhea

EAgEC E. Aggregative - Diarrhea and kids in Chile, Mexico, India, Japan

Serratia -Otitis externa

SalmonellaS. enterica Salmonellosis

Foods -from animal origin →eggs, milk, poultry -Non animal origin → Contaminated H₂O, animal contact (reptiles), human contact

S. typhi Typhoid fever -P-P, fecal cont. food & water

Shigella

A → S. dysenteria -HUSS -produce Shiga toxins- doesn’t ferment mannitol (the rest do)

B → S. flexner -A & B → Common cause of Shigellosis (travelers’ diarrhea) in developing countries-In US → D & B

C → S. bongoriD → S. sonnei

Yersinia

Y. pestisPneumonic plague -Trans → Rat Fleas

-Is a potential Bio-warfare agentBlack death Bubonic plague -Swollen Lymph nodes

Septicemic plague -Septic shock

Y. enterocolitica

-Trans → food, water, blood products (So is listeria)-iliosacral inflammation, mesenteric lymphadenitis, septicemia, polyarthritis, meningitis-Prefers cool temps.

Y. pseudo tuberculosis

scarlet fever-like disease -GI infec, endotoxemia

Key:

Slant / Butt

A → Yellow (+)K → Red (=)

Notes:

1. TSIa. Has Glucose, Sucrose & Lactose

i. Glucose is fermented → K/Aii. All 3 fermented → A/A

2. KIA

a. Has Lactose & Dextrose

i. Dextrose is fermented → K/A Lactose / Sucroseii. Both are fermented → A/A

iii. Neither ferment → K/K3. TD test

a. Lysine, arginine, and ornithineb. = → means all 3 were =

Glucose