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Presentation Short Title 1

College of EngineeringEngineering Education Innovation Center

Engr 1182 Nano Pre-Lab

Intro to Lab-on-a-chip

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Presentation Short Title 2

Lab-on-a-Chip• Small scale chemical lab in a portable and disposable

format.• Require smaller amounts of reagents• Disposable/Recyclable• Manufactured with inexpensive materials

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OSU Research

DNA analysis

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Biomedical Lab-on-a-Chip• A type of Lab-on-a Chip used for Biomedical

diagnostic tests specifically• Biomedical Lab-on-a-Chip is also called a “Point of care” device• Point of care:

– is defined as medical testing at or near the site of patient care– makes the test available to the patient immediately and conveniently – Therefore, physician receives the results quicker and can make

immediate clinical decisions• Examples for “point of care” devices

– Screening for infectious diseases in the emergency room– Forensic analysis at crime scenes– At home screening for HIV, Diabetes, or even Cancer

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• Sample delivery/collection• Targeted compound

– Fluorescein *• Fluidics

– Fluidic channels in the chip• Detection interface

– Detection Alignment Device *• Analysis

– Electric circuit to interpret

detected data• Readout/Display

• Flashing LEDs*

* - These are specific to our Lab-on-a-Chip project.Rev: 20XXMMDD, Initials

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Micro vs. Nano

• Our chip is based on micro-fluidics and thus has micron size channels.

• Our chip has channel widths of 280-400 microns and a depth of 200 microns.

• There are no nano-fluidic devices yet on the market.• If we could manufacture nano-fluidic devices we might

be able to:– Use extremely small amount of samples– Control the flow of individual DNA or other biomolecules on

the chip.

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Our Lab-on-a-chip project

• You will create 2 lab-on-a-chip designs that can detect and measure the concentration of fluorescein.

• What is fluorescein?

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Fluorescein

• Fluorescein in solution emits a “green” light when stimulated with “blue” light. This is called fluorescence.

• Since it isn’t toxic it can be used to track fluid flows and flow rates in the body.

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One Fluorescein Use

• A type of dye that is used in the study of the blood vessels in the eye. The test provides information about the circulatory system and the condition of the retina.

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Leakagebetweenvessels

UnderUltravioletLight

Retinal photograph of a patient complaining of decreased vision.Looks normal.

Fluorescein angiogram indicating fluid leakage within the retina

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Dry Eye detection

• Fluorescein is also used to determine tear flow rate in the eyes as a diagnostic tool for dry eye sydrome.

• After putting fluorescein in the eye, tears coming into the eye will reduce the fluorescein concentration over time.

• The rate of concentration change can be used to determine tear flow rate.

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Objective of Our Chip

• After fluorescein put in the eyes, tear samples are taken at intervals and the concentration must be determined for each sample.

• Our chips must be able to reliably measure the concentration of multiple sequential samples of fluorescein.

Read Project Description Document in the class materials for more details!

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