Engineering BioBrick vectors from BioBrick parts
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Transcript of Engineering BioBrick vectors from BioBrick parts
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Engineering BioBrick vectors from BioBrick parts
Reshma P Shetty, Drew Endy and Thomas F Knight Jr
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Outline Background
Synthetic Biology Terms
Method Current Research Discussion
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Synthetic Biology What is Synthetic Biology?
A Method Milestones
Recombinant DNA PCR(Polymerase Chain Reaction) Automated Sequencing Automated Construction Standards Abstractions
-Write
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Last 30 years
Synthetic Biology
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Synthetic Biology (Cont.) Systems
Devices
Parts
DNA
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Terms Vector Plasmid Polymerase Restriction enzyme Ligase
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Method Goal
Standardize the BioBrick vectors’ assembly process Maintain
Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites
Process Build new BioBrick vectors’ backbone Standard assembly for new BioBrick
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Method(Cont.) Basic BioBrick
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Method(Cont.) Build new BioBrick vectors’ backbone
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Method(Cont.) Standard assembly for new BioBrick
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Method(Cont.) Check
Cloning site Selection marker High copy orgin Terminators flanking the cloning site Verification primer annealing sites
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Current Research More than 200 parts
Some Systems
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Discussion Wetware
Biological film Digital counting machine
Interdisciplinary research
Computer aided design