Engineering Aphid Symbionts for Food Security Kate Elston
Transcript of Engineering Aphid Symbionts for Food Security Kate Elston
1. Engineering Aphid Symbionts for Food Security
Kate Elston
Microbiology, University of Texas at Austin, Austin, TX
2. Managing Data with the Experiment Data Depot
William Morrell1, Mark Forrer2, Garrett Birkel3, Nathan J Hillson4, Hector Garcia-
Martin5, Teresa Lopez6, Tyler Backman7, Chris Petzold8, Edward E. K. Baidoo9, David
Ando10, Zak Costello11, and Ian Vaino12
(1)Biomass Science & Conversion Technologies, Sandia National Laboratory,
Livermore, CA, (2)Biomass Science & Conversion Technology, Sandia National Lab,
Emeryville, CA, (3)Physical Biosciences, Lawrence Berkeley National Laboratory,
Berkeley, CA, (4)Technology Department, DOE Joint BioEnergy Institute,
EMERYVILLE, CA, (5)Biofuels and Bioproducts, Joint BioEnergy Institute, Emeryville,
CA, (6)Sandia National Lab, Emeryville, CA, (7)Joint BioEnergy Institute, Emeryville,
CA, (8)Lawrence Berkeley Nation Lab, Emeryville, CA, (9)Biological Systems and
Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA,
(10)Lawrence Berkeley National Lab, Emeryville, CA, (11)U.S. DOE Agile BioFoundry,
Emeryville, CA, (12)Lawrence Berkeley Nation Lab, Berkeley, CA
3. Development of Cell-Free Protein Expression Biosensors for Detection of Human
Performance Biomarkers
Kathryn Beabout1,2, Amy M. Ehrenworth Breedon1,2, Vaughn Litteral2,3, Svetlana
Harbaugh1, Roland Saldanha3,4, Michael Goodson1, Nancy Kelley-Loughnane5, and
Jorge L. Chávez1
(1)711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson
Air Force Base, OH, (2)Integrative Health and Performance Sciences, UES, Inc.,
Dayton, OH, (3)U.S. Air Force School of Aerospace Medicine, Air Force Research
Laboratory, Wright-Patterson Air Force Base, OH, (4)SSI Inc., assigned to U.S. Air
Force School of Aerospace Medicine, Air Force Research Laboratory, Wright-Patterson
Air Force Base, OH, (5)Materials and Manufacturing Directorate, Air Force Research
Laboratory, Wright-Patterson Air Force Base, OH
4. New genetic tools for DOPA incorporation enable catechol-metalloprotein
engineering
Simon d'Oelsnitz Molecular Biosciences, University of Texas at Austin, Austin, TX
5. Directed Evolution of Antimutator Cells to Stabilize Synthetic Biology Against
Evolution
Dacia Leon, Daniel E. Deatherage, Jenna C. McGuffey, Simon D'Alton, and Jeffrey E.
Barrick Molecular Biosciences, The University of Texas at Austin, Austin, TX
6. Rational Design and Optimization of Ten Orthogonal and Modular Cell-Cell
Communication Systems
Chunbo Lou Institute of Microbiology, Chinese Academy of Science, Beijing, China
7. Harnessing Tardigrade-Specific Intrinsically Disordered Proteins to Enhance the
Robustness of Living Medicines
Danny Collins1, Jonathan Stokes1, Luis R. Soenksen2,3, and James J. Collins4,5
(1)Infectious Disease & Microbiome Program, Broad Institute, Cambridge, MA,
(2)Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA,
(3)Living Celular Devices, Wyss Institute at Harvard, Boston, MA, (4)Biological
Engineering, Massachusetts Institute of Technology, Cambridge, MA, (5)Wyss Institute
for Biologically Inspired Engineering, Harvard University, Boston, MA
8. Phase Space Analysis for Gene Circuit Design
Tim Rudge1,2, Macarena A. Muñoz Silva3, Tamara Matute4, Isaac Nuñez5, Carlos A.
Ruiz6, Ambrosio Valdés6, Gonzalo A. Vidal-Peña1, and Fernan Federici7
(1)Institute for Biological and Medical Engineering, Schools of Engineering, Biology and
Medicine, Pontificial Catholic University of Chile, Santiago, Chile, (2)Department of
Chemical and Bioprocess Engineering, School of Engineering, Pontificial Catholic
University of Chile, Santiago, Chile, (3)School of Life Sciencies, Andrés Bello University,
Santiago, Chile, (4)Pontificia Universidad Católica de Chile, Santiago, Chile,
(5)Engineering, Pontificia Universidad Catolica de Chile, Santiago, Chile, (6)School of
engineering, Pontificial Catholic University of Chile, Santiago, Chile, (7)School of
Biological Sciences, Pontificial Catholic University of Chile, Santiago, Chile
9. A Simple, Robust, and Low-Cost Method to Produce the Pure Cell-Free System
Barbora Lavickova1 and Sebastian J. Maerkl2
(1)Institute of Bioengineering,School of Engineering, École polytechnique fédérale de
Lausanne, Lausanne, Switzerland, (2)Institute of Bioengineering, School of Engineering,
Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
10. Controlling Heterogeneity and Increasing Titer from Riboswitch-Regulated Bacillus
Subtilis spores for Time-Delayed Protein Expression Applications
Denis Tamiev1, Grace Vezeau2, Howard M. Salis3, and Nigel Reuel4
(1)Iowa State University, Ames, IA, (2)Pennsylvania State University, University Park,
PA, (3)Department of Chemical Engineering / Biological Engineering, Pennsylvania
State University, University Park, PA, (4)Chemical and Biological Engineering, Iowa
State University, Ames, IA
11. Scalable Cell-Free Extract Preparation and Minimal Genetic Template Methods for
Rapid Protein Prototyping
Jared Dopp1 and Nigel Reuel2
(1)Iowa State University, Ames, IA, (2)Chemical and Biological Engineering, Iowa State
University, Ames, IA
12. Engineering of Crassulacean Acid Metabolism (CAM) Pathway to Enhance
Photosynthesis and Abiotic Stress Tolerance in C3 Plants
Xiaohan Yang, Degao Liu, Rongbin Hu, and Gerald A. Tuskan
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN
13. 3-D Multicellular Co-Culture Model As a Platform for Rapid Screening of
Engineered Microbial Therapies
Tetsuhiro Harimoto1, Zakary Singer1, Oscar Velazquez1, Joanna Zhang1, Samuel
Castro1, Taylor Hinchliffe1, William Mather2, and Tal Danino1
(1)Biomedical Engineering, Columbia University, New York, NY, (2)University of
California, San Diego, San Diego, CA
14. Capturing Provenance of Designing and Building Biological Systems
John Meng1, Ernst Oberortner1, Nathan J Hillson2, and Jan-Fang Cheng1
(1)DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek,
CA, (2)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA
15. Development of an Optogenetically-Controlled Recombinase for E. coli
Michael Sheets1 and Mary J. Dunlop2
(1)Biomedical Engineering, Boston University, Boston, MA, (2)Department of
Biomedical Engineering, Boston University, Boston, MA
16. Building an Orthogonal Initiation System in a Genomically Recoded Organism
Russel M. Vincent, Bradley M. Wright, and Paul Jaschke
Molecular Sciences, Macquarie University, Sydney, NSW, Australia
17. Building Fungal Factories That Generate Potent Insecticides
Rosannah Cameron1, Kyle Van de Bittner1, Leyla Bustamante1, Rudranuj Bundela1,
Craig van Dolleweerd2, Sarah Kessans3, Arvina Ram4, Barry Scott4, Mathew Nicholson5,
and Emily Parker1
(1)Ferrier Research Institute, Victoria University of Wellington, Wellington, New
Zealand, (2)Protein Science & Engineering, Callaghan Innovation, University of
Canterbury, Christchurch, New Zealand, (3)School of Biological Sciences, University of
Canterbury, Christchurch, New Zealand, (4)Institute of Fundamental Sciences, Massey
University, Palmerston North, New Zealand, (5)Viclink, Victoria University of
Wellington, Wellington, New Zealand
18. Control of Bacterial Communities Using Deep Reinforcement Learning
Neythen J. Treloar1 and Chris P. Barnes2
(1)Cell and Developmental Biology, University College London, London, United
Kingdom, (2)UCL Genetics Institute, University College London, London, United
Kingdom
19. Improving Sakuranetin Biosynthesis Using Metabolically Engineered Microbial Co-
Cultures
Xiaonan Wang, Lizelle Policarpio, and Haoran Zhang
Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ
20. Retrosynthesis of All Available Pathways to Microbial Production of Precursors to
Target Chemicals Based on Chemical Separation Characteristics
Leanne Whitmore1, Anthe George2, and Corey Hudson1
(1)Systems Biology, Sandia National Laboratories, Livermore, CA, (2)Biomass Science
& Conversion Technology, Sandia National Laboratories, Livermore, CA
21. Controlling Protein Expression Levels in Synthetic, Polycistronic Operon Systems
Daniel Gerngross and Sven Panke
Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland
22. Genetic Code Expansion in Bacillus Subtilis
Devon Stork1, Erkin Kuru1, Aditya M. Kunjapur1, Ethan Garner2, and George M
Church3
(1)Department of Genetics, Harvard Medical School, Boston, MA, (2)Harvard
University, Cambridge, MA, (3)Genetics, Harvard Medical School, Boston, MA
23. Synthetic Genomic Contexts in Bacteria for Extreme Expression Properties
Scott Scholz1, Rucheng Diao1, Michael Wolfe1, Elayne Fivenson2, Xiaoxia (Nina) Lin3,
and Peter Freddolino1
(1)Biological Chemistry, University of Michigan, Ann Arbor, MI, (2)Harvard Medical
School, Boston, MA, (3)Chemical Engineering, University of Michigan, Ann Arbor, MI
24. Leveraging a Yeast Display-Based Stop Codon Suppression Reporter for Rapid
Screening of Aminoacyl-tRNA Synthetases
James A. Van Deventer and Jessica T. Stieglitz
Chemical and Biological Engineering, Tufts University, Medford, MA
25. A Broadly Applicable and Integrated Biological Strategy to Induce Ammonia
Excretion from Symbiotic Diazotrophs for Plant Fertilization
Tim Schnabel1 and Elizabeth Sattely2
(1)Department of Bioengineering, Stanford University, Stanford, CA, (2)Department of
Chemical Engineering, Stanford University, Stanford, CA
26. Characterization of the Met25 As a Facile, Color-Associated, and Counter-
Selectable Genetic Marker in Yarrowia Lipolytica
Harley Edwards1 and Peng Xu2
(1)CBEE, University of Maryland, Baltimore County, Baltimore, MD, (2)Chemical,
Biochemical and Environmental Engineering, University of Maryland, Baltimore County,
Baltimore, MD
27. Developing Computational Design Tools for RNA-Based Gene Regulatory Elements
Calvin M. Schmidt and Christina D. Smolke
Stanford University, Stanford, CA
28. Natural-Vanillin Fermentation from Sugar : Production on Industrial Scale
Jun Takakura1, Keita Fukui1, Miku Matsuzawa1, Sayaka Asari1, Risa Yasuda1, Keiko
Noguchi1, Junko Ito1, Keiko Danjo1, Benjamin Mijts2, Christine Roche2, Sheetal Modi2,
Michael Siddiqui2, Peter Kelly2, Matt Davis2, and Rekha Murali2
(1)Ajinomoto, Kawasaki, Japan, (2)Zymergen, Emeyville, CA
29. Nucleic Acid Cleavage with a Hyperthermophilic Cas9 from an Unculturable
Ignavibacterium
Stephanie Tzouanas Schmidt1, Feiqiao Brian Yu1,2,3, Paul C. Blainey4,5, Andrew P.
May3, and Stephen R. Quake1,3,6
(1)Bioengineering, Stanford University, Stanford, CA, (2)Electrical Engineering,
Stanford University, Stanford, CA, (3)Chan Zuckerberg Biohub, San Francisco, CA,
(4)Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA,
(5)Broad Institute of Harvard and MIT, Cambridge, MA, (6)Applied Physics, Stanford
University, Stanford, CA
30. A Fusion Method to Develop an Expanded Artificial Genomic RNA Replicable By
Qbeta Replicase
Kensuke Ueda1, Ryo Mizuuchi2, Fumio Matsuda1, and Norikazu Ichihashi3,4
(1)Department of Bioinformatic Engineering, Graduate School of Information Science
and Technology, Osaka University, Suita, Osaka, Japan, (2)Department of Chemistry,
Portland State University, Portland, OR, (3)Department of Life Science, Graduate School
of Arts and Science, The University of Tokyo, Meguro, Tokyo, Japan, (4)Komaba
Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan
31. An Efficient Platform for Standardized Genome Engineering in Bacteria
Carolyn Bayer1, Maja Rennig1,2, Anja Ehrmann1, and Morten H.H. Nørholm1,2,3
(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark, (3)CloneOpt
AB, Upplands Väsby, Sweden
32. Using Triplex-Forming Oligos (TFOs) to Repress Promoter Activity in Chinese
Hamster Ovary (CHO) Cells
Mohamed K. Hussein1,2, Martina Baumann2, Heena Dhiman2, Ly Nguyen2, Neža Novak2,
Sybille Galosy3, and Nicole Borth1
(1)Department of Biotechnology, University of Natural Resources and Life Sciences,
Vienna, Austria, (2)ACIB, Austrian Centre of Industrial Biotechnology, Vienna, Austria,
(3)GSK, GlaxoSmithKline, King of Prussia, PA
33. Engineered Mammalian Bioluminescence Enzymes - the First Sensitive Glowing
Mouse Reporter Gene for Biomedicine
James A. Long, Amit P. Jathoul, and James A. H. Murray
BIOSI, Cardiff University, Cardiff, United Kingdom
34. A Synthetic Live Bacterial Therapeutic Organism for the Treatment of the Human
Metabolic Disease Phenylketonuria (PKU)
Vincent Isabella1, David Lubkowicz1, Sarah Rowe1, Binh Ha1, Mary Castillo1, Cami
Anderson1, Paul Miller1, Marja Puurrunen2, and Caroline Kurtz1
(1)Synlogic Inc., Cambridge, MA, (2)Synlogic, Cambridge, MA
35. Synthetic Sequence Entanglement Augments Stability and Containment of Genetic
Information in Cells
Hsing-I Ho, Tomasz Blazejewski, and Harris Wang
Department of Systems Biology, Columbia University, New York, NY
36. Synthetic Symbiosis
Can Huang1, Jasmine Olivares1, Han Wang1, Gerardo Amores2, Paul de Figueiredo2,
and Arum Han1
(1)Texas A&M University, College Station, TX, (2)Texas A&M Health Science Center,
Bryan, TX
37. Programmable Gene Activation in Bacteria
Jennifer Fang1, Hsing-I Ho2, and Harris Wang2
(1)Department of Biological Sciences, Columbia University, New York, NY,
(2)Department of Systems Biology, Columbia University, New York, NY
38. Learning Protein Structure-Function Relationships from Data
Zhiyuan Duan Department of Biochemistry, University of Wisconsin Madison, Madison, WI
39. Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring
Resistance to the Antimalarial Drug Fosmidomycin in E. coli
Gur Pines1, Eun Joong Oh1, Marcelo Bassalo1,2, Alaksh Choudhury1, Andrew Garst3,
Reilly Fankhauser1, Carrie Eckert4,5, and Ryan T. Gill1
(1)University of Colorado Boulder, Boulder, CO, (2)Massachusetts Institute of
Technology, Cambridge, MA, (3)Inscripta, Boulder, CO, (4)Renewable and Sustainable
Energy Institute (RASEI), University of Colorado, Boulder, CO, (5)National Renewable
Energy Laboratory (NREL), Golden, CO
40. A Synthetic RNA-Mediated Evolution System in Yeast
Emil Damgaard Jensen1, Tadas Jakociunas2, Michael K. Jensen3, and Jay Keasling4
(1)The Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kgs. Lyngby, Denmark, (2)Technical University of Denmark, The Novo
Nordisk Foundation Center for Biosustainability, Kgs. Lyngby, Denmark, (3)Novo
Nordisk Foundation Center for Biosustainability, Technical University of Denmark,
Kongens Lyngby, Denmark, (4)Biological Systems and Engineering Division, Lawrence
Berkeley National Laboratory, Emeryville, CA
41. Expanding Boundaries of Synthetic Biology: Discovery and High-Throughput
Characterisation of Inducible Gene Expression Systems
Erik Hanko1, Ana Paiva2, Nigel Minton3, and Naglis Malys1
(1)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences,
University of Nottingham, Nottingham, United Kingdom, (2)Centre for Biomolecular
Sciences, School of Life Sciences, University of Nottingham, Nottingham, United
Kingdom, (3)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences,
The University of Nottingham, Nottingham, United Kingdom
42. Measuring the Efficiency of CRISPR Genome-Editing Systems with Single-Cell
DNA Analysis
Keith W. Jones, Mani Manivannan, Shu Wang, Pedro Mendez, and David Ruff
Mission Bio, South San Francisco, CA
43. Multiplex Transcriptional Characterizations across Diverse Bacterial Species Using
Cell-Free Systems
Sung Sun Yim1, Nathan Johns2, and Harris Wang3
(1)Systems biology, Columbia University, New York, NY, (2)Systems Biology, Columbia
University Medical Center, New York, NY, (3)Department of Systems Biology, Columbia
University, New York, NY
44. Predictable Tuning of Synthetic Microbial Consortium Pulse Generator Dynamics
David Zong1, Mehdi Sadeghpour2, William Ott2, Krešimir Josić2, and Matthew R.
Bennett3
(1)PhD Program in Systems Synthetic and Physical Biology, Rice University, Houston,
TX, (2)Department of Mathematics, University of Houston, Houston, TX, (3)Department
of Bioengineering, Rice University, Houston, TX
45. Bere: Bayesian Quality-Estimator for Reproducible Biological Experiments
Shuowei Li1 and Eric Klavins2
(1)Electrical and Computer Engineering, University of Washigton, Seattle, WA,
(2)Electrical and Computer Engineering, University of Washington, Seattle, WA
46. Single Cell Chemical Imaging with Stimulated Raman Scattering for Biofuel
Production Screening
Nathan Tague1, Jean-Baptiste Lugagne1, Haonan Lin1, Fengyuan Deng1, Wilson Wong1,
Ji-Xin Cheng1, and Mary J. Dunlop2
(1)Biomedical Engineering, Boston University, Boston, MA, (2)Department of
Biomedical Engineering, Boston University, Boston, MA
47. Towards Life with Fewer Than 20 Amino Acids
Liyuan Liu, Tomasz Blazejewski, Christian Munck, Florencia Velez-Cortes, and Harris
Wang
Department of Systems Biology, Columbia University, New York, NY
48. Design and Application of S-Adenosylmethionine Methyltransferase Growth-
Coupling for Evolutionary Engineering
Anne Sofie L. Hansen1, Jakob Hjort Schmidt1, Denis Shepelin1, Lei Yang1, Mette
Kristensen1, Mohammad Radi1, Hanne B. Christensen1, Adam Feist1,2, Jay D.
Keasling1,3,4,5,6, Bernhard O. Palsson1,2,7, Michael K. Jensen1, Markus J. Herrgård1, and
Hao Luo1
(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kongens Lyngby, Denmark, (2)Department of Bioengineering, University of
California - San Diego, La Jolla, CA, (3)Joint BioEnergy Institute (JBEI), Emeryville,
CA, (4)Biological Systems & Engineering Division, Lawrence Berkeley National
Laboratory, Berkeley, CA, (5)Center for Synthetic Biochemistry, Institute for Synthetic
Biology, Shenzhen Institutes of Advanced Technologies, Shenzhen, China, (6)Department
of Chemical and Biomolecular Engineering & Department of Bioengineering, University
of California, Berkeley, CA, (7)Department of Pediatrics, University of California - San
Diego, La Jolla, CA
49. Expression of RNA Origami Scaffolds in Saccharomyces Cerevisiae for
Transcriptional Control
Georgios Pothoulakis, Guido Grossi, and Ebbe S. Andersen
Interdisciplinary Nanoscience Center and Department of Molecular Biology and
Genetics, Aarhus University, Aarhus, Denmark
50. Cytosolic Folding Biosensors for Discovery of Abeta42 and Alpha-Synuclein Folding
Factors in Saccharomyces Cerevisiae
Veronika Sachsenhauser1, Hyun-hee Kim2, Xiexiong Deng2, and James Bardwell2
(1)Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor,
Ann Arbor, MI, (2)Molecular, Cellular, and Developmental Biology, HHMI, University
of Michigan, Ann Arbor, MI
51. Metabolic Engineering of Oleaginous Yeast Yarrowia Lipolytica for Production of
Flavor Lactones
Eko Roy Marella1, Jonathan Dahlin2, Marie I. Dam1, Carina Holkenbrink1, Guokun
Wang2, and Irina Borodina1
(1)The Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kongens Lyngby, Denmark, (2)The Novo Nordisk Center for Biosustainability,
Technical University of Denmark, Kongens Lyngby, Denmark
52. Challenging the Workhorse: Comparative Analysis of Eukaryotic Microorganisms
for Expressing Monoclonal Antibodies
Hanxiao Jiang1, Andrew Horwitz1, Chapman Wright2, Anna Tai1, Elizabeth
Znameroski1, Yoseph Tsegaye1, Hailley Warbington1, Benjamin Bower1, Christina
Alves2, Carl Co2, Kanvasri Jonnalagadda2, Darren Platt1, Jessica Walter1, Venkatesh
Natarajan2, Jeff Ubersax1, Joel R. Cherry1, and J. Christopher Love3
(1)Amyris, Emeryville, CA, (2)Biogen, Cambridge, MA, (3)Department of Chemical
Engineering, The David H. Koch Institute for Integrative Cancer Research,
Massachusetts Institute of Technology, Cambridge, MA
53. The Development of Leucine Consuming Strains As Therapeutics for Maple Syrup
Urine Disease
JR Gao1, Ning LI2, Chris Bergeron1, Lauren Renaud1, Mylene Perreault1, Pat
Cantarella1, Mike James1, Mary Castillo2, David Lubkowicz2, John Thomas1, Paul
Miller2, Caroline Kurtz2, Alex Tucker3, Alex Carlin3, Silvia Galvan3, Ryan Putman3,
Kolea Zimmerman3, Rishi Jain3, and Scott Marr3
(1)Synlogic, Cambridge, MA, (2)Synlogic Inc., Cambridge, MA, (3)Ginkgo, Boston, MA
54. An Electrofermentation Platform for Diterpene Production
Emily Lanier, Michaela TerAvest, and Björn Hamberger
Biochemistry & Molecular Biology, Michigan State University, Lansing, MI
55. Structural and Sequential Analysis Comparing Residues between Functionally
Divergent Terpene Synthases in Prunella vulgaris
Garret P. Miller, Sean R. Johnson, Wajid Waheed Bhat, Radin Sadre, Alekzander Sky
Garcia, and Björn Hamberger
Biochemistry & Molecular Biology, Michigan State University, Lansing, MI
56. Patterns of Microbial Colonization in the Murine Gut Reveals Design Rules for
Robust Microbial Engraftment
Ravi U. Sheth and Harris Wang
Department of Systems Biology, Columbia University, New York, NY
57. Engineered Bacteria to Assess and Augment Human Performance: Expanding
Sensing Capabilities
Amy M. Ehrenworth Breedon1,2, Kathryn Beabout1,2, Christina Davis1,2, Mao Taketani3,
Christopher A. Voigt3, Jorge L. Chávez2, Nancy Kelley-Loughnane4, and Michael
Goodson2
(1)Integrative Health and Performance Sciences, UES, Inc., Dayton, OH, (2)711th
Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force
Base, OH, (3)Synthetic Biology Center, Department of Biological Engineering,
Massachusetts Institute of Technology, Cambridge, MA, (4)Materials and Manufacturing
Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, OH
58. Just Add DNA: Occam Cloning Facilitates One-Step and Scarless In Vivo Assembly
of Expression Vectors
Maja Rennig1,2, Carolyn Bayer1, Anja Ehrmann1, and Morten H.H. Nørholm1,2
(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark
59. Autonomous Platform for Protein Design
Jacob Rapp1, Bennett Bremer2, and Philip A. Romero3
(1)Biochemistry, University of Wisconsin-Madison, Madison, WI, (2)Biochemistry,
University of Wisconsin - Madison, Madison, WI, (3)Department of Biochemistry,
University of Wisconsin-Madison, Madison, WI
60. Deep Learning for RNA Synthetic Biology
Nicolaas M. Angenent-Mari1,2, Luis R. Soenksen3,4, Alexander S. Garruss5, Katherine
M. Collins6, Diogo M. Camacho7, George M Church8, Timothy K Lu9, and James J
Collins10
(1)Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA,
(2)Living Cellular Devices, 2Wyss Institute for Biologically Inspired Engineering,
Boston, MA, (3)Mechanical Engineering, Massachusetts Institute of Technology,
Cambridge, MA, (4)Living Celular Devices, Wyss Institute at Harvard, Boston, MA,
(5)Living Machines, Wyss Institute for Biologically Inspired Engineering, Boston, MA,
(6)Electrical Engineering and Computer Science, Massachusetts Institute of Technology,
Cambridge, MA, (7)System Discovery, Wyss Institute for Biologically Inspired
Engineering, Boston, MA, (8)Genetics, Harvard Medical School, Boston, MA,
(9)Biological Engineering and Electrical Engineering and Computer Science, MIT,
Boston, MA, (10)Wyss Institute for Biologically Inspired Engineering, Harvard
University, Boston, MA
61. Controlled Assembly of RNA-Protein Nanostructures in Escherichia coli
Michael T. Nguyen1, Georgios Pothoulakis1, Cody Geary2, and Ebbe S. Andersen1
(1)Interdisciplinary Nanoscience Center and Department of Molecular Biology and
Genetics, Aarhus University, Aarhus, Denmark, (2)Bioengineering department,
California Institute of Technology, Pasadena, CA
62. Rapid Construction of Highly Multiplexed Gene Pools
Nathan Raynard, Elian Lee, Siyuan Chen, and Rebecca Nugent
Twist Bioscience, San Francisco, CA
63. Leveraging Twist Matrixed Oligo Pools for Multiplexed Gene Editing
Elian Lee, Rebecca Nugent, and Siyuan Chen
Twist Bioscience, San Francisco, CA
64. Creating Diverse Combinatorial Pathways with High-Throughput DNA Assembly
Nathan Raynard, Siyuan Chen, and Rebecca L Nugent
Twist Bioscience, San Francisco, CA
65. Improving Desiccation Tolerance in Synechococcus Sp. PCC 7002 Towards
Regeneration of Biomaterials
Juliana Artier1, Jishen Qiu2, Sarah L. Williams2, Mija H. Hubler2, Wil V. Srubar III2,
Sherri M. Cook2, and Jeffrey C. Cameron1,3,4
(1)Renewable & Sustainable Energy Institute (RASEI), University of Colorado Boulder,
Boulder, CO, (2)Department of Civil, Environmental, and Architectural Engineering,
University of Colorado Boulder, (3)Department of Chemistry and Biochemistry,
University of Colorado Boulder, (4)National Renewable Energy Laboratory (NREL),
Golden, CO
66. Engineering Multi-Input/Single-Output Systems in Yeast for Neural Network
Computation
Alberto Carignano1, Cannon Mallory1, and Eric Klavins2
(1)Electrical Engineering, University of Washington, Seattle, WA, (2)Electrical and
Computer Engineering, University of Washington, Seattle, WA
67. Development of Synthetic Biology Kits for STEM Education
Andrew Walters1, Ron Sims1, Ryan Jackson2, and Charles Miller1
(1)Biological Engineering, Utah State University, Logan, UT, (2)Chemistry and
Biochemistry, Utah State University, Logan, UT
68. A Tunable Integrase Differentiation Circuit Improves Output of Burdensome
Functions
Rory Williams1 and Richard M. Murray2
(1)Bioengineering, Caltech, Pasadena, CA, (2)Division of Biology and Biological
Engineering, California Institute of Technology, Pasadena, CA
69. Living Foundations: Building Hierarchical Materials with Synthetic Biology
Marimikel Charrier1, Dong Li1, Victor Mann1, Lisa Yun2, Sneha Jani1, Behzad Rad1,
Bruce Cohen3, Paul Ashby1, Kathleen Ryan4, and Caroline Ajo-Franklin1
(1)The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA,
(2)Michigan State University, East Lansing, MI, (3)Lawrence Berkeley National
Laboratory, Berkeley, CA, (4)Department of Plant & Microbial Biology, University of
California, Berkeley, CA
70. High Throughput Approaches to Dissecting Gene Regulation of Biosynthetic Gene
Clusters
Jimin Park Department of Systems Biology, Columbia University, New York, NY
71. High-Throughput Anaerobic Platform to Turbocharge Strain Engineering of Gas
Fermenting Microorganisms
Fungmin (Eric) Liew1, Michael Köpke2, and Sean D. Simpson2
(1)Synthetic Biology, LanzaTech Inc, Skokie, IL, (2)LanzaTech, Inc., Skokie, IL
72. Synthetic Biology Approaches for Improving Production of Semi-Synthetic
Artemisinin
Stephanie H. Kung, Hanxiao Jiang, Abhishek Murarka, Timothy Dobbs, Sean Lund, Will
Corcoran, and Christopher J. Paddon
Amyris, Emeryville, CA
73. Broad Loading Substrate Specificity, Diverse Extenders, and Defined ß-
Oxidation for a Platform Polyketide Synthase
Amin Zargar1, Ravi Lal2, Jessica Wang2, Miranda Werts2, Andrew Wong2, Luis
Valencia2, Constance Bailey2, Veronica T. Benites3, Edward E. K. Baidoo3, Leonard
Katz4, and Jay Keasling5
(1)Chemical Engineering, UC Berkeley, Berkeley, CA, (2)The Joint BioEnergy Institute,
Emeryville, CA, (3)Biological Systems and Engineering Division, Lawrence Berkeley
National Laboratory, Berkeley, CA, (4)QB3, University of California, Berkeley, Berkeley,
CA, (5)Biological Systems and Engineering Division, Lawrence Berkeley National
Laboratory, Emeryville, CA
74. Continuous Directed Evolution of Tryptophan Synthase Toward Production of
Unnatural Aromatic Amino Acids
Gordon Rix University of California Irvine, Irvine, CA
75. Cell-Free System in Aqueous Two-Phase Enables Multiplexing of Small Molecule
and Nucleic Acids
Yan Zhang1, Taisuke Kojima2, Shuichi Takayama2, and Mark P. Styczynski1
(1)School of Chemical and Biomolecular Engineering, Georgia Institute of Technology,
Atlanta, GA, (2)Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA
76. Expanding the Diversity of Reporters for Use in Multiplexed Cell-Free Protein
Synthesis Systems for Paper Based Sensing Platforms
Caitlin Sharpes1,2, John McManus1, Steven Blum1, James Myslinski1, Glory Mgboji3,
Vanessa Funk1, Stephanie Cole1, Peter Emanuel1, and Matthew Lux1
(1)CCDC Chemical Biological Center, Aberdeen Proving Ground, MD, (2)Excet, Inc.,
Springfield, VA, (3)Oak Ridge Institute for Science and Education, Oak Ridge, TN
77. Engineering Mammalian Cells to Record Their Own History
Courtney Carlson Biomedical Engineering, UC Irvine, Irvine, CA
78. Engineering of Lactobacillus Reuteri As a Biotherapeutic Delivery System
Annie Goodwin1, Laura Ortiz2, Laura Schafer1, Melinda Engevick1, Heather Danhof1,
Jeff Tabor3, and Robert Britton4
(1)Baylor College of Medicine, Houston, TX, (2)Rice University, houston, TX,
(3)Department of Bioengineering, Rice University, Houston, TX, (4)MVM, Baylor
College of Medicine, Houston, TX
79. Anti-CRISPR-Mediated Control of Gene Editing and Synthetic Circuits in
Eukaryotic Cells
Muneaki Nakamura1 and Stanley L. Qi2
(1)Stanford University, Stanford, CA, (2)Bioengineering, Chemical and Systems Biology,
Stanford, Stanford, CA
80. Development of a Commercially Available Targeted Integration CHOZN® gs -/-
Cell Lin
James Ravellette1, Mike Johns1, Jason Tuter1, Joaquina Mascarenhas1, Trissa
Borgschulte2, and Scott Bahr1
(1)Cell Sciences and Development, MilliporeSigma, St. Louis, MO, (2)Process Solutions,
MilliporeSigma, St. Louis, MO
81. Proteomics-Driven Genome Remodeling to Boost Cellular Capacity for Engineered
Functions
Adam Chazin-Gray Pacific Northwest National Laboratory, Richland, WA
82. Optimal Protein Reclamation with Maxmass
Neeraj Kumar1, Jeremy D. Zucker1, Jeremy Teuton1, Robert Egbert2, and Joseph
Cottam3
(1)Computational Biology, Pacific Northwest National Laboratory, Richland, WA,
(2)Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA,
(3)Visual Analytics, Pacific Northwest National Laboratory, Richland, WA
83. Development of a CRISPR-Mediated Methodology to Re-Write Microalgal
Genomes
Alexandra Mystikou1, Weiqi Fu2, David Nelson2, and Kourosh Salehi-Ashtiani1
(1)Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi,
Abu Dhabi, United Arab Emirates, (2)Division of Science and Math, New York
University Abu Dhabi, Abu Dhabi, United Arab Emirates
84. A Cell-Free Expression System for the Site-Specific Incorporation of Natural Amino
Acid Probes
Annika Urbanek1, Anna Morató1, Matija Popovic1, Carlos A. Elena-Real1, Frédéric
Allemand1, Aurélie Fournet1, Ana M. Gil2, Anabel I. Jiménez2, Carlos Cativiela2,
Stéphane Delbecq3, Nathalie Sibille1, and Pau Bernadó1
(1)Centre de Biochimie Structurale, Montpellier, France, (2)Department of Organic
Chemistry/ISQCH, University of Zaragoza-CSIC, Zaragoza, Spain, (3)Faculté de
Pharmacie, Université de Montpellier, Montpellier, France
85. Rewiring the Bacterial Genome By Retromutagenesis for Tolerance Development in
Microbial Cell Factories
Sophia A. H. Heyde and Morten H.H. Nørholm
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark,
Kgs. Lyngby, Denmark
86. CRISPR-cas9 Platform Development for a Wild-Type White-Rot Fungus Cerrena
Unicolor BBP6
Hao Zhang1 and Anli Geng2
(1)Ngee Ann Polytechnic, Singapore, Singapore, (2)School of Life Sciences and
Technology, Ngee Ann Polytechnic, Singapore, Singapore
87. Development of an Insoluble Protein Biosensor and Application in Yeast Cell
Factories
David Romero-Suarez1, Yixin Rong2, Tadas Jakociunas3, Michael K. Jensen4, and Jay
Keasling5,6,7,8,9
(1)DTU Biosustain, Technical University of Denmark, Kgs. Lyngby, Denmark, (2)Novo
Nordisk Foundation Center for Biosustainability, Kongens Lyngby, Denmark,
(3)Technical University of Denmark, The Novo Nordisk Foundation Center for
Biosustainability, Kgs. Lyngby, Denmark, (4)Novo Nordisk Foundation Center for
Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark,
(5)Biological Systems and Engineering Division, Lawrence Berkeley National
Laboratory, Emeryville, CA, (6)Department of Chemical and Biomolecular Engineering
& Department of Bioengineering, University of California, Berkeley, Berkeley, CA,
(7)Center for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes
for Advanced Technologies, Shenzhen, China, (8)Joint BioEnergy Institute, Department
of Energy, Emeryville, CA, (9)The Novo Nordisk Foundation Center for
Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark
88. Discovering Druggable Gene Combinations for Parkinson's Disease by CombiGEM-
CRISPR
Ka Ching Chan1 and Alan Siu Lun Wong1,2
(1)School of Biomedical Sciences, The University of Hong Kong, Hong Kong, Hong
Kong, (2)Department of Electrical and Electronic Engineering, The University of Hong
Kong, Hong Kong, Hong Kong
89. Synthetic Enzymology: Directed Computational Evolution of Quorum-Quenching
Lactonases
Maybelle Kho Go1,2, Bo Xue1,2, Lina Zhao3, Robert C. Robinson4, Hao Fan3, and Wen
Shan Yew1,2
(1)Biochemistry, National University of Singapore, Singapore, Singapore, (2)NUS
Synthetic Biology for Clinical and Technological Innovation, Singapore, Singapore,
(3)Bioinformatics Institute, A*STAR, Singapore, Singapore, (4)Okayama University,
Okayama, Japan
90. Engineering the Human Compass Family of Histone H3K4 Methylases in Yeast
Max Haase and Jef Boeke
Institute for Systems Genetics, NYU Langone Health, New York, NY
91. Design and Rapid Emulation of a Synthetic Microbial Operational Amplifier on
Cytomorphic Silicon Chips
Jonathan Teo1, JI Zeng2, Sung Sik Woo3, and Rahul Sarpeshkar2
(1)Massachusetts Institute of Technology, Cambridge, MA, (2)Dartmouth College,
Hanover, NH, (3)Formlabs, Somerville, MA
92. Natural and Directed Evolution of Substrate Specificity in Family 5 Glycoside
Hydrolases
Evan Glasgow1,2, Kirk Vander Meulen1,2, Taichi E. Takasuka1,2,3, Christopher
Bianchetti1,2,4, Lai Bergeman1,2, Samuel Deutsch5, and Brian G. Fox1,2
(1)Great Lakes Bioenergy Research Center, Madison, WI, (2)Biochemistry, University of
Wisconsin - Madison, Madison, WI, (3)Hokkaido University, Sapporo, Japan,
(4)Chemistry, University of Wisconsin - Oshkosh, Oshkosh, WI, (5)DOE Joint Genome
Institute, Walnut Creek, CA
93. Cell Free Transcription-Translation for Natural Product Research
Patrick D. Capel, Christophe Corre, and Emzo de los Santos
University of Warwick, Coventry, United Kingdom
94. Whole-Cell Living Bioreporters for Butanol Production: Directed Evolution of a
Transcriptional Regulator Towards Short-Chain Alcohols
Maximilian O. Bahls and Sven Panke
Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland
95. Beacon Riboregulators: Exploiting Loop-Mediated RNA-RNA Interactions for
Molecular Computing and Diagnostics
Duo Ma The Biodesign Institute and the School of Molecular Sciences, Arizona State University,
Tempe, AZ
96. Simultaneous Regulation of Many Genes Using Crispri with Highly Non-Repetitive
Extra Long Sgrna Arrays (ELSAs)
Alexander Reis1, Sean Halper1, Grace Vezeau2, Daniel Cetnar1, Ayaan Hossain3, Phillip
Clauer4, and Howard M. Salis5
(1)Chemical Engineering, Penn State University, University Park, PA, (2)Pennsylvania
State University, University Park, PA, (3)Bioinformatics and Genomics, Penn State
University, University Park, PA, (4)Biochemistry and Molecular Biology, Pennsylvania
State University, University Park, PA, (5)Department of Chemical Engineering /
Biological Engineering, Pennsylvania State University, University Park, PA
97. Synchronising a Population of Yeast Cells By Microfluidics-Based Feedback
Control
Giansimone Perrino1, Davide Fiore2, Sara Napolitano3,4, Mario di Bernardo5,6, and
Diego di Bernardo1,4
(1)Telethon Institute of Genetics and Medicine, Pozzuoli, Italy, (2)Electric Engineering
and Information Technology, University of Naples Federico II, Naples, Italy, (3)Telethon
Institute of Genetics and Medicine, Naples, Italy, (4)Chemical, Materials and Industrial
Production Engineering, University of Naples Federico II, Naples, Italy, (5)Department
of Electrical Engineering and Information Technology, University of Naples Federico II,
Naples, Italy, (6)University of Bristol, Bristol, United Kingdom
98. Dynamic Control of Pathway Expression with Riboregulated Switchable Feedback
Promoters
Cameron J. Glasscock1, Tyler Lazar2, Bradley W. Biggs2, Jack Arnold2, Min-Kyoung
Kang2, Danielle Tullman-Ercek2, Keith E.J. Tyo2, and Julius B. Lucks2
(1)Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell
University, Ithaca, NY, (2)Chemical and Biological Engineering, Northwestern
University, Evanston, IL
99. Diagnosing Limitations to High Titer Ethanol Production in Clostridium
Thermocellum Using a Cell-Free System
Daniel Olson1, Jingxuan Cui1, Daniel Amador-Noguez2, and Lee R. Lynd3
(1)Thayer School of Engineering, Dartmouth College, Hanover, NH, (2)Bacteriology,
University of Wisconsin - Madison, Madison, WI, (3)Thayer School of Engineering,
Department of Biological Sciences, Dartmouth College, Hanover, NH
100. Automated DNA Construction: From j5 Protocol Design to Laboratory
Robotics
Nurgul Kaplan1,2,3, Tadeusz Ogorzalek1,2,3, Joel M. Guenther4, Garima Goyal5, Jennifer
Chiniquy1,2, Jonathan Diab1,2,6, Henrique C. De Paoli1, and Nathan J Hillson1,2,3
(1)DOE Agile BioFoundry, EMERYVILLE, CA, (2)Biological Systems and Engineering
Division, Lawrence Berkeley National Laboratory, Berkeley, CA, (3)Technology
Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA, (4)Sandia National
Laboratory, Livermore, CA, (5)Fuels Synthesis Division, Joint BioEnergy Institute,
Emeryville, CA, (6)Technology Division, DOE Joint BioEnergy Institute, EMERYVILLE,
CA
101. Validation and Feedback Control of a Reference-Comparator System within
Escherichia coli
Barbara Shannon, Fabio Annunziata, Gianfranco Fiore, Antoni Matyjaszkiewicz, Claire
Grierson, Lucia Marucci, Nigel Savery, and Mario di Bernardo
University of Bristol, Bristol, United Kingdom
102. High-Throughput Genome Engineering for Industrial Strain Optimization
Michael Martyn III Platform R&D, Zymergen, Emeryville, CA
103. Engineering Prototrophy in Mammalian Cells
Julie Trolle1, Ross McBee2, Liyuan Liu2, Andrew Kaufman2, Xinyi Guo1, Sudarshan
Pinglay1, Henri Berger1, Sergei German1, Harris Wang2, and Jef Boeke1
(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)Department of
Systems Biology, Columbia University, New York, NY
104. Mapping Ribozyme Fitness Landscapes to Investigate Evolutionary
Optimization and Divergence
Evan Janzen Biomolecular Science and Engineering, University of California, Santa Barbara, Santa
Barbara, CA
105. Sugar Synthesis from CO2 in Escherichia coli
Shmuel Gleizer1, Niv Antonovsky1, Elad Herz1, Elad Noor2, Yehudit Zohar1, Arren Bar-
Even3, and Ron Milo1
(1)Weizmann Institute of Science, Rehovot, Israel, (2)Institute of Molecular Systems
Biology, ETH Zurich, Zurich, Switzerland, (3)Systems and Synthetic Metabolism, Max
Planck Institute for Molecular Plant Physiology, Potsdam, Germany
106. Engineered History-Dependent Behavior in a Multicellular System
Ana Zuñiga1, Sarah Guiziou1, Ben Meriem Zacchary2, Pauline Mayonove1, Violaine
Moreau1, Luca Ciandrini3, Pascal Hersen2, and Jerome Bonnet1
(1)Centre de Biochimie Structurale, Montpellier, France, (2)Laboratoire Matière et
Systèmes Complexes, Paris, France, (3)Laboratoire Charles Coulomb, Montpellier,
France
107. Tunning Cross Membrane Signal Transmission for Synthetic Receptor
Engineering
HungJu Chang1, Jerome Gracy1, Pauline Mayonove1, Martin Cohen-Gonsaud1,
Guillaume Cambray2, and Jerome Bonnet1
(1)Centre de Biochimie Structurale, Montpellier, France, (2)INRA, UMR DGIMI,
Montpellier, France
108. Human Milk Oligosaccharides - Enabling Novel Health Benefit Applications
through the Glycoactives Platform
Joeri Beauprez1 and Wesley Carpentier2
(1)Inbiose N.V., Zwijnaarde, Belgium, (2)Inbiose NV, Zwijnaarde, Belgium
109. Engineering Asymmetrical Cell Division into Escherichia coli
Sara Molinari Department of Biosciences, Rice University, Houston, TX
110. Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space
of Cell-Free Biosensors
Peter Voyvodic1, Amir Pandi2, Ismael Conejero1,3,4, Emmanuel Valjent5, Philip
Courtet3,6, Eric Renard5,7, Jean-Loup Faulon2,8, and Jerome Bonnet1
(1)Centre de Biochimie Structurale, Montpellier, France, (2)MICALIS Institute, INRA,
Jouy-en-Josas, France, (3)INSERM U1061, Neuropsychiatry, Epidemiological and
Clinical Research, Montpellier, France, (4)Department of Psychiatry, Caremeau
Hospital, University Hospital of Nîmes, Nîmes, France, (5)Institute of Functional
Genomics, UMR CNRS 5203/INSERM U1191, Montpellier, France, (6)Department of
Emergency Psychiatry and Post Acute Care, CHU Montpellier, Lapeyronie Hospital,
Montpellier, France, (7)Department of Endocrinology, Diabetes, Nutrition and CIC,
Montpellier University Hospital, Montpellier, France, (8)SYNBIOCHEM Center,
University of Manchester, Manchester, United Kingdom
111. Refurbishing the Fluorescence Complementation Assay Landscape - Design
and Construction of Mammalian Expression Vector Sets for Improved Signal-to-
Noise Ratios in Protein-Protein-Interaction Analyses
Raluca Fleischer1,2 and Gary R. Lewin3
(1)Max-Delbrück-Zentrum, Berlin, Germany, (2)Humboldt-Universität zu Berlin, Berlin,
Germany, (3)Max-Delbrück-Zentrum
112. High Throughput Strain Engineering at Ginkgo Bioworks
Alicja Gomes Ginkgo Bioworks, Boston, MA
113. A Proteome Allocation Model to Improve Photosynthetic Productivity and
Efficiency in a Light-Limited Chemostat
Marjan Faizi and Ralf Steuer
Institute for Theoretical Biology, Humboldt Universität zu Berlin, Berlin, Germany
114. Molecular Sequestration Strategies for Quorum Sensing Systems
Nicholas DeLateur1, Christian E. Cuba Samaniego2, and Ron Weiss3
(1)Department of Biological Engineering, M.I.T., Cambridge, MA, (2)Department of
Biological Engineering, MIT, Cambridge, MA, (3)Biological Engineering, M.I.T.,
Cambridge, MA
115. Exploring Genetic Variation Using Retron Library Recombineering (RLR)
Max G. Schubert1, Daniel B. Goodman2, Seth Shipman3, and George M Church4
(1)Harvard Medical School, Boston, MA, (2)Wyss Institute for Biologically Inspired
Engineering, Boston, MA, (3)Gladstone Institutes, San Francisco, CA, (4)Genetics,
Harvard Medical School, Boston, MA
116. Identification and Experimental Manipulation of Surface Colonization
Pathways in a Model Marine Diatom
Weiqi Fu1, Amphun Chaiboonchoe1, Mehar Sultana2, Kristos Baffour1, Dina Al-Khairy1,
Sarah Daakour1, Alexandra Mystikou2, Amnah Alzahmi1, Ashish Jaiswal1, David Nelson1,
Sigurdur Brynjolfsson3, and Kourosh Salehi-Ashtiani2
(1)Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, United
Arab Emirates, (2)Center for Genomics and Systems Biology (CGSB), New York
University Abu Dhabi, Abu Dhabi, United Arab Emirates, (3)Center of Systems Biology,
University of Iceland, Iceland
117. Personalized Off-Target Analysis of CRISPR-Cas9 gRNA Designs Using in-
Silico and Experimental Approaches
Yidan Pan, Ciaran Lee, and Gang Bao
Department of Bioengineering, Rice University, Houston, TX
118. Increasing Functionality of GFP in Human Cells
Cansu Kuey, Gabrielle Larocque, Nicholas Clarke, and Stephen Royle
Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry, United
Kingdom
119. Multiplexing Cell-Cell Communication
John T. Sexton and Jeffrey J. Tabor
Department of Bioengineering, Rice University, Houston, TX
120. Cell-Free Styrene Biosynthesis at High Titers
Blake J. Rasor1,2, William S. Grubbe3, Ashty S. Karim1,2, and Michael C. Jewett1,2,4
(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,
(2)Center for Synthetic Biology, Northwestern University, Evanston, IL, (3)Institute for
Molecular Engineering, University of Chicago, Chicago, IL, (4)Chemistry of Life
Processes Institute, Northwestern University, Evanston, IL
121. Engineered Probiotic for the Inhibition of Salmonella Via Tetrathionate-
Induced Production of Microcin H47
Benedikt Mortzfeld, Jacob Palmer, and Vanni Bucci
Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA
122. "Big DNA" Construction and Characterization of Alpha-Globin Locus
Variants
Brendan R. Camellato1, Leslie A. Mitchell1, Helena Francis2, Mira T. Kassouf2, Matthew
T. Maurano1, Douglas R. Higgs2, and Jef Boeke1
(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)MRC
Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United
Kingdom
123. Engineering Cupriavidus Necator for Autotrophic 1,3-Butanediol Production
Joshua Gascoyne1, Rajesh Bommareddy2, Stephan Heeb1, and Naglis Malys1
(1)Synthetic Biology Research Centre, Centre for Biomolecular Sciences, University of
Nottingham, Nottingham, United Kingdom, (2)Synthetic Biology Research Centre, Centre
for Biomolecular Sciences, University of Nottingham, United Kingdom
124. Molecular Crowders Regulate Gene Expression in an E. coli-Based Cell-Free
System
Grace Vezeau and Howard Salis
Pennsylvania State University, University Park, PA
125. Massively-Parallel Dissection of Human Transcription Factor Binding Site
Architecture
Jessica Davis1, Kimberly Insigne2, Eric M. Jones3, Quinn Hastings4, and Sriram Kosuri5
(1)Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA,
(2)Bioinformatics IDP, UCLA, Los Angeles, CA, (3)Octant, Los Angeles, CA, (4)UCLA,
Los Angeles, CA, (5)Department of Chemistry and Biochemistry, University of
California, Los Angeles, Los Angeles, CA
126. Software-Enable Design and Automated Construction of CRISPR Gene
Circuits in S. Cerevisiae
Justin Vrana1 and Eric Klavins2
(1)Bioengineering, UW-Seattle, Seattle, WA, (2)Electrical and Computer Engineering,
University of Washington, Seattle, WA
127. An Engineered Light-Switchable Two-Component System for Dynamic
Interrogation of Cell-Fate Decision Networks in Bacillus Subtilis
Sebastian M. Castillo-Hair1, Elliot A. Baerman2,3, Masaya Fujita4, Oleg Igoshin1, and
Jeffrey J. Tabor1
(1)Department of Bioengineering, Rice University, Houston, TX, (2)Rice University,
Houston, TX, (3)Baylor College of Medicine, Houston, TX, (4)University of Houston,
Houston, TX
128. Optimal Experimental Design for a Bistable Gene Regulatory Network
Nathan Braniff, Addison Richards, and Brian Ingalls
University of Waterloo, Waterloo, ON, Canada
129. Interfacing a Transcriptional Biosensing with Toehold-Mediated Strand
Displacement for Programmable Molecular Diagnostics
Jaeyoung K. Jung1,2, Khalid K. Alam1,2, and Julius B. Lucks1,2,3,4
(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,
(2)Center for Synthetic Biology, Northwestern University, Evanston, IL,
(3)Interdisciplinary Biological Sciences Program, Northwestern University, Evanston,
IL, (4)Chemistry of Life Processes Institute, Northwestern University, Evanston, IL
130. Acoustic Detonation of Cellular Therapies Via Biomolecular Cavitation
Avinoam Bar-Zion1, Atousa Nourmahnad1, David Mittelstein2, Sangjin Yoo1, Dina
Malounda1, Mohamad Abedi3, Audrey Lee-Gosselin1, David Maresca1, and Mikhail G.
Shapiro1
(1)Division of Chemistry and Chemical Engineering, California Institute of Technology,
Pasadena, CA, (2)Division of Engineering and Applied Science, California Institute of
Technology, Pasadena, CA, (3)Division of Biology and Biological Engineering,
California Institute of Technology, Pasadena, CA
131. Programmable Polyketide Synthase (PKS) Platform and an Artificial
Biosynthetic Pathway for the Natural Food Pigment Carmine
Rasmus John Norman Frandsen1, Kresten Jon Kromphardt Olsen2, Paiman Khorsand-
Jamal3, Kenneth T. Kongstad4, Majse Nafisi5, Rubini Kannangara6, Birger Lindberg
Møller7, Thomas Ostenfeld Larsen2, and Uffe H. Mortensen1
(1)DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark,
(2)DTU Bioengineering, Technical University of Denmark, Kgs. Lyngby, Denmark,
(3)Technical University of Denmark, Kgs. Lyngby, Denmark, (4)Department of Drug
Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark, (5)Chr.
Hansen Natural Colors A/S, Hoersholm, Denmark, Horsholm, Denmark, (6)Chr. Hansen
Natural Colors A/S, Hoersholm, Denmark, (7)Copenhagen Plant Science Centre,
Department of Plant and Environmental Sciences, University of Copenhagen,
Copenhagen, Denmark
132. Single-Cell Characterization of Gene Regulatory Functions in the S.
Cerevisiae Inorganic Phosphate Starvation Response Network
Evan J. Olson1, Hon Ming Yip1, Michael A. Crone2, Shiyu Cheng1, and Sebastian J.
Maerkl1
(1)Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de
Lausanne, Lausanne, Switzerland, (2)Department of Medicine, Imperial College London,
London, United Kingdom
133. High-Throughput Microfluidic Platform for Gene Regulatory Network
Characterization at the Single Cell Level
Hon Ming Yip1, Evan J. Olson1, Michael A. Crone2, Shiyu Cheng1, and Sebastian J.
Maerkl1
(1)Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de
Lausanne, Lausanne, Switzerland, (2)Department of Medicine, Imperial College London,
London, United Kingdom
134. Metabolic Engineering of Bacillus Subtilis for Biopolymer Production from
Biodiesel Waste Material
Lorenzo Pasotti1, Ilaria Massaiu1, Erlinda Rama2, Matteo Cavaletti2, Nikolaus
Sonnenschein3, Markus J. Herrgård4, Paolo Magni1, and Cinzia Calvio5
(1)Dep. of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia,
Italy, (2)Biology and Biotechnology, University of Pavia, Pavia, Italy, (3)The Novo
Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs.
Lyngby, Denmark, (4)Novo Nordisk Foundation Center for Biosustainability, Technical
University of Denmark, Kongens Lyngby, Denmark, (5)Department of Biology and
Biotechnology, University of Pavia, Pavia, Italy
135. Screening for Peptide Inhibitors of S. typhimurium PhoPQ in E. coli
Kathryn Brink1, Andrew Mu2, Bryan Davies3, and Jeff Tabor4
(1)PhD Program in Systems, Synthetic, and Physical Biology, Rice University, Houston,
TX, (2)Department of Biosciences, Rice University, Houston, TX, (3)Department of
Molecular Biosciences, UT Austin, Austin, TX, (4)Department of Bioengineering, Rice
University, Houston, TX
136. Beyond Hill Equations: Mechanistic Modeling of Inducible Systems to
Expand the Predictability of Synthetic Circuits
Davide De Marchi1, Lorenzo Pasotti2, Massimo Bellato2, and Paolo Magni2
(1)Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy,
(2)Dep. of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia,
Italy
137. How Resource Competition Shapes the Stability Profile of Toggle Switches at
the Cellular and at the Population Levels
Andras Gyorgy Electrical Engineering, New York University Abu Dhabi, Abu Dhabi, United Arab
Emirates
138. Carbon Monoxide Tolerance and Utilisation in Cupriavidus Necator
Charlie Wickham-Smith1, Naglis Malys2, and Klaus Winzer3
(1)Synthetic Biology Research Centre, University of Nottingham, Nottingham, United
Kingdom, (2)Synthetic Biology Research Centre, Centre for Biomolecular Sciences,
University of Nottingham, Nottingham, United Kingdom, (3)School of Life Sciences,
University of Nottingham, Nottingham, United Kingdom
139. Prototyping Protein-Protein Interaction-Based Logic Using Cell-Free Protein
Synthesis
Andrew Hunt1, Zibo Chen2, David Baker2, and Michael C. Jewett1
(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,
(2)Department of Biochemistry, University of Washington, Seattle, WA
140. CRISPR-Cas Based Capture of Horizontal Gene Transfer in Complex
Communities
Christian Munck1, Ravi U. Sheth1, Danie E. Freedberg2, and Harris Wang1
(1)Department of Systems Biology, Columbia University, New York, NY, (2)Division of
Digestive and Liver Diseases, Columbia University Medical Center, New York, NY
141. Combinatorial Design of Protein Switches for Controlling Electron Transfer
in Cells
Bingyan Wu1, Joshua T. Atkinson2, George N. Bennett3, and Jonathan J. Silberg4
(1)Biosciences, Rice University, Houston, TX, (2)Systems, Synthetic, and Physical
Biology Graduate Program, Rice University, Houston, TX, (3)Department of Biosciences,
Rice University, Houston, TX, (4)Biosciences and Bioengineering, Rice University,
Houston, TX
142. A Synthetic Genomics Approach to Dissecting an Age-Related Macular
Degeneration-Associated Haplotype
Jon M Laurent1, Xin Fu2, Sergei German1, Ran Brosh1, Kang Zhang2, Matthew T.
Maurano1, and Jef Boeke1
(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)Shiley Eye
Institute, University of California San Diego School of Medicine, San Diego, CA
143. Engineering Multicellular 3D Shapes
Jesse Tordoff1, Matej Krajnc2, Nicholas Walczak3, Jacob Beal3, Stanislav Y.
Shvartsman2, and Ron Weiss4
(1)CSB, MIT, Cambridge, MA, (2)Chemical and Biological Engineering, Princeton
University, Princeton, NJ, (3)Raytheon BBN Technologies, Cambridge, MA,
(4)Biological Engineering, M.I.T., Cambridge, MA
144. Development of a Gene Signal Amplifier Platform Technology for
Monitoring the Unfolded Protein Response
Carlos A. Origel1, Bhagyashree K. Bachhav2, and Laura Segatori3
(1)Bioscience Department, Rice University, Houston, TX, (2)Department of Chemical
and Biomolecular Engineering, Rice University, Houston, TX, (3)Bioengineering, Rice
University, Houston, TX
145. Engineering a Gas-Producing Biosensor to Monitor Nitrate Bioavailiability
in Terrestrial Soils and Marine Sediments
Emily M. Fulk1, Ilenne Del Valle1, Xiaodong Gao2, Caroline A. Masiello3, and Jonathan
J. Silberg4
(1)Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, (2)Earth,
Environmental and Planetary Sciences, Rice University, Houston, TX, (3)Earth Sciences,
Rice University, Houston, TX, (4)Biosciences and Bioengineering, Rice University,
Houston, TX
146. Independent Control of Mean and Noise By Convolution of Gene Expression
Distributions
Karl Gerhardt1, Evan J. Olson2, and Jeff Tabor1
(1)Department of Bioengineering, Rice University, Houston, TX, (2)Institute of
Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne,
Lausanne, Switzerland
147. Application of Terminator, Protein Degradation, and Protein Solubility
Libraries for Strain Improvement
Matthew Davis, Chris Wisnewski, and Patrick Westfall
Zymergen, Emeryville, CA
148. A High-Throughput Assay to Investigate the Antibiotic Activity of the Lasso
Peptide Klebsidin
Ethan Hills, Benjamin Brandsen, and Stanley Fields
Department of Genome Sciences, University of Washington, Seattle, WA
149. Synthetic Biologic Tools for Microbial Ecology and Biogeochemistry
David L. Shis1, Ilenne Del Valle2, Emily M. Fulk3, Johnathan J. Silberg4, and Caroline A.
Masiello5
(1)Department of Biosciences, Rice University, Houston, TX, (2)Systems, Synthetic, and
Physical Biology, Rice University, Houston, TX, (3)Chemical and Biological
Engineering, Northwestern University, Evanston, IL, (4)Biochemistry & Cell Biology,
Rice University, Houston, TX, (5)Earth Sciences, Rice University, Houston, TX
150. Exploring the Sequence Space of a Riboswitch Expression Platform Yields
Tunable Switching Efficacy
Gregory W. Campbell Biomolecular Science and Engineering, University of California, Santa Barbara, Santa
Barbara, CA
151. Mining Pams from Diverse Bacterial Genomes
Florencia Velez-Cortes and Harris Wang
Department of Systems Biology, Columbia University, New York, NY
152. Evolutionary Design of Synthetic Oligomers and Protein-Based Materials
Anna J Simon Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX
153. Using Microbial Memory to Record Horizontal Gene Transfer Events in
Situ.
Prashant Kalvapalle1, Emily M. Fulk2, Johnathan J. Silberg3, and Lauren Stadler4
(1)Systems, Synthetic and Physical Biology, Rice University, HOUSTON, TX, (2)Systems,
Synthetic, and Physical Biology, Rice University, Houston, TX, (3)Biochemistry & Cell
Biology, Rice University, Houston, TX, (4)Civil and Environmental Engineering, Rice
University, Houston, TX
154. Developments of Genetic Tools for Methane-Oxidizing Bacteria
Bashir Rumah1, Christopher Stead1, Nigel Minton2, and Ying Zhang1
(1)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, The
University of Nottingham, Nottingham, United Kingdom, (2)BBSRC/EPSRC Synthetic
Biology Research Centre, University of Nottingham, University of Nottingham, United
Kingdom
155. Recombinase Efficiency in Human Cell Lines
George Chao1, Clair Travis1, and George M Church2
(1)Harvard Medical School, Boston, MA, (2)Genetics, Harvard Medical School, Boston,
MA
156. Metabolic Engineering of Yarrowia Lipolytica for De Novo Synthesis of 2-
Phenylehtanol from Sustainable Low-Cost Feedstocks
Yang Gu1, Long Liu2, and Peng Xu3
(1)University of Maryland, Baltimore county, Baltimore, MD, (2)Jiangnan university,
Wuxi, China, (3)Chemical, Biochemical and Environmental Engineering, University of
Maryland, Baltimore County, Baltimore, MD
157. Metabolic engineering of Yarrowia lipolytica for de novo synthesis of 2-
phenylehtanol from sustainable low-cost feedstocks Yang Gu1,2, Long Liu2 and Peng Xu1
1 Chemical, Biochemical and Environmental Engineering, UMBC, Baltimore, USA 2School of Biotechnology, Jiangnan University, Wuxi, China
158. Control of Protein Synthesis By Using Promoter Variants in Cell-Free
System
Naoko Senda1, Ruolan Zhang2, and Hirokazu Nishida3
(1)Research & Development Group, Hitachi, Ltd., Tokyo, Japan, (2)Hitachi, Ldt., Tokyo,
Japan, (3)Hitachi, Ltd., Tokyo, Japan
159. Characterization of an Integrase-Based Genetic ‘Tape Recorder’
Circuit in e coli
Andrey Shur1 and Richard M. Murray2
(1)Biology and Biological Engineering, California Institute of Technology, Pasadena,
CA, (2)Division of Biology and Biological Engineering, California Institute of
Technology, Pasadena, CA
160. Engineering Gut Inflammation Biosensors with Bacterial Stress Response
Kathleen Jia Yue Zhang1 and David R. McMillen2
(1)Cell and Systems Biology, University of Toronto, Toronto, ON, Canada, (2)Chemical
and Physical Sciences, University of Toronto, Toronto, ON, Canada
161. A Synthetic Approach for Parsing Gene Regulatory Logic in Plant
Development
Edith Pierre-Jerome and Philip Benfey
Biology, Duke University, Durham, NC
162. Synthetic Enzymology and the Fountain of Youth
Yan Ping Lim1, Maybelle Kho Go1, Matthew Wook Chang2, and Wen Shan Yew3
(1)NUS Synthetic Biology for Clinical and Technological Innovation, Singapore,
Singapore, (2)Department of Biochemistry, Yong Loo Lin School of Medicine, National
University of Singapore, Singapore, Singapore, (3)Biochemistry, National University of
Singapore, Singapore, Singapore
163. Variational Autoencoder for Generation of Antimicrobial Peptides
Scott N. Dean1 and Scott A. Walper2
(1)National Research Council, Washington, DC, (2)Center for Biomolecular Science and
Engineering, Naval Research Laboratory, Washington, DC
164. Response of Lactobacillus Plantarum WCFS1 to the Pathogen-Associated
Quorum Sensing Molecule N-3-Oxododecanoyl Homoserine Lactone
Joseph R. Spangler1, Scott N. Dean1, Dagmar Leary2, and Scott A. Walper2
(1)National Research Council, Washington, DC, (2)Center for Biomolecular Science and
Engineering, Naval Research Laboratory, Washington, DC
165. Characterization of Membrane-Bound Components in Cell-Free
Glycosylation Systems
Katherine F. Warfel1, Jasmine M. Hershewe1, Han Teng Wong1, Justin Peruzzi1, Neha
Kamat2, Danielle Tullman-Ercek1, and Michael C. Jewett1
(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,
(2)Biomedical Engineering, Northwestern University, Evanston, IL
166. Metabolic Engineering of Corynebacterium Glutamicum Strains for the
Production of Bio-Based Nylon Monomers
Si Jae Park1, Hee Taek Kim2, and Jeong Chan Joo3
(1)Division of Chemical Engineering and Materials Science, Ewha Womans University,
Seoul, Korea, Republic of (South), (2)KRICT, Daejeon, Korea, Republic of (South),
(3)Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research
Institute of Chemical Technology, Daejeon, Korea, Republic of (South)
167. Metagenomic Platform for Rapid Natural Product Discovery
Aleksandr Milshteyn Discovery, Zymergen Inc., Emeryville, CA
168. Regulatory Aspects of Synthetic Biology in Germany
Wolfram Volkwein, Ulrich Busch, and Armin Baiker
Bavarian Health and Food Safety Authority, Oberschleißheim, Germany
169. Development of a Complete CRISPR-Cpf1 Tool for Metabolic Engineering
of Clostridium Beijerinckii ncimb 8052
Constantinos Patinios Microbiology, Wageningen University and Research, Wageningen, Netherlands
170. Synthetic Orfeomes of Prochlorococcus MarinusMED4 and NATL1A: A
Resource for Systems-Level Interrogation of Low- and High-Light Adaptations in
Cyanobacteria
Sarah Daakour1,2, Weiqi Fu1, Ashish Jaiswal1, Amnah Alzahmi1,2, Joseph Koussa3,
Amphun Chaiboonchoe1, David Nelson1,2, and Kourosh Salehi-Ashtiani1,2
(1)Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, United
Arab Emirates, (2)Center for Genomics and Systems Biology (CGSB), New York
University Abu Dhabi, Abu Dhabi, United Arab Emirates, (3)Department of Biology,
New York University Abu Dhabi, New York, NY
171. Genetically-Encoded RNA Origami Nanostructures and Nanodevices to
Scaffold Proteins
Guido Grossi1, Cody Geary1,2, Ilenia Manuguerra1, Paul W. K. Rothemund2, and Ebbe S.
Andersen1
(1)Interdisciplinary Nanoscience Center and Department of Molecular Biology and
Genetics, Aarhus University, Aarhus, Denmark, (2)Bioengineering department,
California Institute of Technology, Pasadena, CA
172. Packaging of Diisopropyl Fluorophosphatase (DFPase) in Bacterial Outer
Membrane Vesicles Protects Its Activity at Extreme Temperature
Meghna Thakur1 and Scott A. Walper2
(1)College of Science, George Mason University, Fairfax, VA, (2)Center for
Biomolecular Science and Engineering, Naval Research Laboratory, Washington, DC
173. Programmable Protein Circuits in Living Cells
Xiaojing Gao1, Lucy Chong1, Matthew Kim1, and Michael Elowitz2
(1)Caltech, Pasadena, CA, (2)California Institute of Technology, Pasadena, CA
174. Sensing Intracellular Metabolites with RNA Nanostructures in E. coli
Ilenia Manuguerra, Mette D. E. Jepsen, Tenna T. Henriksen, Steffen M. Sparvath, and
Ebbe S. Andersen
Interdisciplinary Nanoscience Center and Department of Molecular Biology and
Genetics, Aarhus University, Aarhus, Denmark
175. Towards in Situ Engineering of Electroautotrophic Microbial Communities
Sarah Glaven Center for Bio/Molecular Science and Engineering, Naval Research Laboratory,
Washington, DC
176. Designing Vesicle Membranes to Control Spatial-Temporal Dynamics of
Biological Reactions
Justin Peruzzi1, Miranda Jacobs2, Timothy Vu3, and Neha Kamat3,4
(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,
(2)Interdisciplinary Biological Sciences, Northwestern University, Evanston, IL,
(3)Biomedical Engineering, Northwestern University, Evanston, IL, (4)Center for
Synthetic Biology, Northwestern University, Evanston, IL
177. Chi.Bio: A Low-Cost Platform for Automated Characterisation and
Manipulation of Biological Systems
Harrison Steel1, Robert Habgood2, and Antonis Papachristodoulou1
(1)Department of Engineering Science, University of Oxford, Oxford, United Kingdom,
(2)University of Oxford, Oxford, United Kingdom
178. Hydrogel Delivery of Cell-Free Protein Synthesis
Marilyn F. S. Lee1, Chia-Suei Hung2, Daniel A. Phillips3, Zachary Martinaeu4, Chelsea
Buck5, Maneesh Gupta6, and Matthew Lux7
(1)Biochemistry, US Army CCDC CBC, Aberdeen Proving Ground, MD, (2)Air Force
Research Laboratory, Dayton, OH, (3)US Naval Research Laboratory, Washington, DC,
(4)UES, Inc., Air Force Research Laboratory, Dayton, OH, (5)UES Inc., Air Force
Research Laboratory, Dayton, OH, (6)Materials and Manufacturing Directorate, Air
Force Research Laboratory, WPAFB, OH, (7)CCDC Chemical Biological Center,
Aberdeen Proving Ground, MD
179. Bioconversion of Methane to Transportation Fuel Using Environmentally
Isolated Methanotrophic Bacteria
Christopher Stead, Bashir Rumah, Nigel Minton, and Ying Zhang
BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, The
University of Nottingham, Nottingham, United Kingdom
180. Expression and Purification of Highly Active Antimicrobial Peptide HBCM2
from Escherichia coli Using an Encapsulin Nanocompartment System
Tek-Hyung Lee1, Timothy S. Carpenter1, David Savage2, and Mimi C. Yung1
(1)Lawrence Livermore National Laboratory, Livermore, CA, (2)MCB and Chemistry,
UC Berkeley, Berkeley, CA
181. Temperature Sensitive Variants of the Protease, Subtilisin Carlsberg, for
Automated Processes
Vanessa C. Thompson, Tyler W. Dyer, Geoff Gudavicius, Bailey E McGuire, Max Legg,
and Francis E Nano
Biochemistry & Microbiology, University of Victoria, Victoria, BC, Canada
182. Linking Mobile Genes with Their Bacterial Hosts in Natural Gut
Communities Using OIL PCR
Peter Diebold Microbiology, Cornell University, Ithaca, NY
183. Web of Registries
Hector Plahar1, Leanne Springthorpe2, William Morrell3, Paul D. Adams4, and Nathan J
Hillson5
(1)Fuels Synthesis and Technology Divisions, Joint BioEnergy Institute, Emeryville, CA,
(2)Fuels Synthesis and Technology, Joint Bioenergy Institute, Emeryville, CA,
(3)Biomass Science & Conversion Technologies, Sandia National Laboratory,
Livermore, CA, (4)Technology Division, Joint BioEnergy Institute, Emeryville, CA,
(5)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA
184. Making Hot E. coli Hybrids: Combining Multiple Genetic Approaches for
Directed Evolution
Bailey E McGuire, Quinn E Matthews, and Francis E Nano
Biochemistry & Microbiology, University of Victoria, Victoria, BC, Canada
185. Miniaturization and Rapid Processing of Txtl Reactions Using Acoustic
Liquid Handling
Jared Bailey1, Evelyn Eggenstein2, Jean-Marie Rouillard2, and John Lesnick1
(1)Labcyte, San Jose, CA, (2)Arbor Biosciences, Ann Arbor, MI
186. Combining Scalable DNA Assembly Using the Next Universal Guided
Enzymatic (NUGE) Assembly Method with Automated Workflows
Jared Bailey1, John Lesnick1, and Rebecca L Nugent2
(1)170 Rose Orchard Way, Labcyte Inc, San Jose, CA, (2)Twist Bioscience, San
Francisco, CA
187. Autonomously Controlled and Host-Aware Recombinant Gene Expression
through Plasmid Copy Number Modulation
Nicolas Kylilis and Guy-Bart Stan
Department of Bioengineering, Imperial College London, London, United Kingdom
188. Developing a Modular Framework for Orthogonal Control of Gene
Expression in Plants
Shaunak Kar1, Yogendra Bordiya1, Nestor Rodriguez1, Elizabeth Gardner1, Junghyun
Kim1, Jimmy Gollihar2, Andrew Ellington1,3, and Sibum Sung1
(1)Molecular Biosciences, University of Texas at Austin, Austin, TX, (2)US Army
Research Laboratory- South, Austin, TX, (3)Center for Systems and Synthetic Biology,
University of Texas at Austin, Austin, TX
189. Scaling Semi-Synthetic Artemisinin Production from Plates to Industrial
Fermentation
Timothy Dobbs, Abhishek Murarka, Hanxiao Jiang, Stephanie H. Kung, Daniel Yim, and
Christopher J. Paddon
Amyris, Emeryville, CA
190. In Vitro Implementation of a Noise Filtering Gene Network
Pascal A. Pieters1, Ardjan J. van der Linden1, Jongmin Kim2, Peng Yin2, and Tom F.A.
de Greef1,3
(1)Department of Biomedical Engineering, Eindhoven University of Technology,
Eindhoven, Netherlands, (2)Wyss Institute for Biologically Inspired Engineering,
Harvard University, Boston, MA, (3)Institute for Molecules and Materials, Radboud
University, Nijmegen, Netherlands
191. CRISPR-Cas12 Mediated Genome-Editing in Oleaginous Yeas
Zhiliang Yang1, Harley Edwards2, and Peng Xu2
(1)Chemical, Biochemical and Environmental Engineering, University of Maryland,
Baltimore County, Baltimore, MD, (2)University of Maryland Baltimore County,
Baltimore, MD
192. Engineering Two Component Redox Sensors for Use in Marine Bacteria
Elizabeth Onderko1, Megalya Marquez-Lopez2, and Sarah Glaven3
(1)National Research Council, Washington, DC, (2)The Washington Center for
Internships and Academic Seminars, Washington, DC, (3)Center for Bio/Molecular
Science and Engineering, Naval Research Laboratory, Washington, DC
193. Building a Cas12a Toolkit to Facilitate Manipulation of the Transcriptome
James Bryson School of Biological sciences, University of Edinburgh, Edinburgh, United Kingdom
194. Melanin Biomaterials Produced By Engineered Bacteria
Chia-Suei Hung1, Zachary E. Reinert1,2, Maria Sanford1,2, Andrea R. Poole1,2, Cheri
Hampton1,2, Pamela Lloyd1,2, Annika Vaia1, Lawrence F. Drummy1, Matthew B.
Dickerson1, Wendy J. Crookes-Goodson1, Maneesh K. Gupta1, and Nancy Kelley-
Loughnane1
(1)Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-
Patterson Air Force Base, OH, (2)UES, Inc., Dayton, OH
195. Paper-Based, Cell-Free Detection of Heavy Metals for in-Field Water Quality
Testing
Casey B. Bernhards1, Kathryn Beabout2, Kendrick Turner3, Jorge L. Chávez4, Scott A.
Walper5, and Matthew Lux6
(1)CCDC Chemical Biological Center (Excet, Inc.), Aberdeen Proving Ground, MD,
(2)Integrative Health and Performance Sciences, UES, Inc., Dayton, OH, (3)NRL,
Washington, DC, (4)711th Human Performance Wing, Air Force Research Laboratory,
Wright-Patterson Air Force Base, OH, (5)Center for Biomolecular Science and
Engineering, Naval Research Laboratory, Washington, DC, (6)CCDC Chemical
Biological Center, Aberdeen Proving Ground, MD
196. Plasmid Vectors for In Vivo Selection Free Use with the Probiotic E. coli
Nissle
Anton Kan1, Ilia Gelfat2, Sivaram Emani3, and Neel Joshi4
(1)Wyss Institute, Harvard University, Boston, MA, (2)John A. Paulson School Of
Engineering And Applied Sciences, Harvard University, Cambridge, MA, (3)Harvard
University, Boston, MA, (4)Wyss Institute for Biologically Inspired Engineering, Harvard
University, Boston, MA
197. Cell-Free Systems for the Deployment of Biosensors in Resource-Limited
Environments
Steven Blum1, Glory Mgboji2, Aleksandr Miklos3, Peter Emanuel1, and Matthew Lux1
(1)CCDC Chemical Biological Center, Aberdeen Proving Ground, MD, (2)ORISE, Oak
Ridge, TN, (3)Excet, Inc., Springfield, VA
198. Engineering Ionotropic Chemogenetic Receptors in Yeast
Elizabeth Gardner1, Boris Zemelman2, Andrew Ellington1, and Jimmy Gollihar3
(1)Molecular Biosciences, University of Texas at Austin, Austin, TX, (2)University of
Texas Austin, Austin, TX, (3)US Army Research Laboratory- South, Austin, TX
199. Mosaic Polymers of the Plague Capsular Antigen (Caf1) Protein:
Engineering a Multi-Functional Biomaterial
Daniel T. Peters1, Gema Dura2, Helen Waller1, Adrian Yemm3, Neil D. Perkins3, David
A. Fulton2, Mark A. Birch4, and Jeremy H. Lakey1
(1)Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon
Tyne, United Kingdom, (2)School of Natural and Environmental Sciences, Newcastle
University, Newcastle upon Tyne, United Kingdom, (3)Institute for Cell and Molecular
Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom,
(4)Cambridge University, Cambridge, United Kingdom
200. The Impact of Genome Organization on the Regulation of Gene Expression
in E. coli
Anja Ehrmann1 and Morten H.H. Nørholm1,2
(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of
Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark
201. microRNA Mediated Endothelial Progenitor Function in Chronic Liver
Injury
Nan Wu1, Elise Slevin2, Heather Francis2, Gianfranco Alpini2, and Fanyin Meng1
(1)Roudebush VA Medical Center, Indiana Center for Liver Research, Indiana University
School of Medicine, Indianapolis, IN, (2)Indiana Center for Liver Research,
Indianapolis, IN
202. Reconstruction of Complex Fitness Landscapes Using High-Throughput
Experimental Data
Juannan Zhou and David McCandlish
Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring
Harbor, NY
203. Developing a Dynamic, High Affinity Scaffold Toolkit for the Control of
Intracellular Spatial Organization of Proteins and Metabolic Flux
Alexander Mitkas1 and Wilfred Chen2
(1)Chemical and Biomolecular Engineering, Universtiy of Delaware, Newark, DE,
(2)Chemical Engineering, University of Delaware, Newark, DE
204. Assembly of Synthetic Bacterial Microcompartments in Saccharomyces
Cerevisiae
Yong Quan Tan Biochemistry, National University of Singapore, Singapore, Singapore
205. Characterization of Gene Expression Regulated By Combined Action of a
Chemical Inducer and Temperature
Abhilash Patel, Soumyadip Banerjee, Pranjal Gupta, Nishant Kumar Sharma, and
Shaunak Sen
Electrical Engineering, IIT Delhi, New Delhi, India
206. Open-Source Paper-Fluidic Device for Bacterial Culture, Communication
and Biocomputation
Gonzalo A. Vidal-Peña1 and Tim Rudge1,2
(1)Institute for Biological and Medical Engineering, Schools of Engineering, Biology and
Medicine, Pontificial Catholic University of Chile, Santiago, Chile, (2)Department of
Chemical and Bioprocess Engineering, School of Engineering, Pontificial Catholic
University of Chile, Santiago, Chile
207. Noise-Reducing Optogenetic Circuits in Mammalian Cells
M. Tyler Guinn1 and Gabor Balazsi2
(1)Biomedical Engineering, Stony Brook School of Medicine, Stony Brook, NY,
(2)Biomedical Engineering, Stony Brook University, Stony Brook, NY
208. Application of Optimal Experimental Design to Omics Experimentation
Xiaokang Wang, Navneet Rai, Beatriz Pereira, Ameen Eetemadi, and Ilias Tagkopoulos
University of California-Davis, Davis, CA
209. DNA Construct Sequence Validation Using the Miseq Platform
Jonathan Diab1, Jennifer Chiniquy2, Zak Costello3, Tadeusz Ogorzalek1, Xiaoxian Liu4,
Mark Kulawik5, Cindi Hoover6, Joel M. Guenther7, Nurgul Kaplan1, Garima Goyal8,
Hector Plahar9, Brian Bushnell6, Samuel Deutsch10, and Nathan J Hillson1
(1)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA, (2)DOE
Agile BioFoundry, EMERYVILLE, CA, (3)U.S. DOE Agile BioFoundry, Emeryville, CA,
(4)Joint BioEnergy Institute, Emeryville, CA, (5)Operations, Joint BioEnergy Institute,
Emeryville, CA, (6)Joint Genome Institute, Lawrence Berkeley National Laboratory,
Walnut Creek, CA, (7)Sandia National Laboratory, Livermore, CA, (8)Fuels Synthesis
Division, Joint BioEnergy Institute, Emeryville, CA, (9)Fuels Synthesis and Technology
Divisions, Joint BioEnergy Institute, Emeryville, CA, (10)DOE Joint Genome Institute,
Walnut Creek, CA
210. Design and Synthesis of RNA Thermoregulators to Control Metabolic Flux
in the Production of Essential Metabolites and Therapeutic Molecules
Jose Ruben Morones1 and Albert I Lerma Escalera2
(1)Centro de Investigación en Biotecnología y Nanotecnología, Universidad Autónoma
de Nuevo León, Apodaca, Nuevo León, NL, Mexico, (2)Centro de Investigacion en
Biotecnologia y Nanotecnologia, Universidad Autonoma de Nuevo Leon, Monterrey, NL,
Mexico
211. Improving Electron Flux in E. coli By Altering the Periplasmic Electron
Carriers
Lin Su1,2 and Caroline Ajo-Franklin2
(1)State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China, (2)The
Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA
212. Engineering Transcriptional Interference for NAND and NOR Logic
Behaviors
Nolan O'Connor, Antoni E. Bordoy, and Anushree Chatterjee
Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
213. Novel Cell-Free Protein Synthesis System of Chitinases from Serratia
Marcescens DB11
Michael Huang1, Jayashree Chakravarty2, Christopher Brigham3, and Tracie Ferreira4
(1)Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA,
(2)Bioengineering, University of Massachusetts Dartmouth, NORTH DARTMOUTH,
MA, (3)Interdisciplinary Engineering, Wentworth Institute of Technology, Boston, MA,
(4)University of Massachusetts Dartmouth, NORTH DARTMOUTH, MA
214. Isolating Live Cells after High-Throughput, Long-Term, Time-Lapse
Microscopy
Scott Luro Systems Biology, Harvard Medical School, Boston, MA
215. Engineering Synthetic Biotic MedicinesTM for the Treatment of Cancer
Ning LI, Daniel Leventhal, Kip West, Chris Plescia, Adam Fisher, Carey Gallant,
Starsha Kolodziej, Rudy Christmas, Anna Sokolovska, Michael James, Mary Castillo,
Paul Miller, and Jose Lora
Synlogic Inc., Cambridge, MA
216. Minimal Genomes: In-Silico using Whole-Cell Models, in-Vivo using
CRISPR-cas9
Joshua Rees Life Sciences, University of Bristol, Bristol, United Kingdom
217. Synthetic Biology Platform for Modifying Glycosylation of
Biopharmaceuticals
Christopher Stach1, Meghan G. McCann2, Conor O’Brien3, Tung S. Le4, Wei-Shou Hu2,
and Michael J. Smanski5
(1)Department of Biochemistry, Molecular Biology, and Biophysics, University of
Minnesota, Twin Cities, Minneapolis, MN, (2)Department of Chemical Engineering and
Materials Science, University of Minnesota, Twin Cities, Minneapolis, MN,
(3)Department of Chemical Engineering and Materials Science, University of Minnesota,
Minneapolis, MN, (4)Chemical Engineering and Materials Science, University of
Minnesota, Twin Cities, Minneapolis, MN, (5)Department of Biochemistry, Molecular
Biology, and Biophysics, University of Minnesota, Twin Cities, St. Paul, MN
218. Analysis of Macromolecular Crowding Effects on Gene Expression in E. coli
Cell-Free Systems
Ti Wu Chemistry and Biochemistry, UC San Diego, La Jolla, CA
219. Improving Energy Supplies in E. coli Cell-Free Systems with Polyphosphate
Kinase 2
Ti Wu Chemistry and Biochemistry, UC San Diego, La Jolla, CA
220. Data-Driven Cellular Capacity Optimization through Proteome Reclamation
Robert Egbert Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA
221. An Automated Droplet-Based Microfluidic System for Optimizing
Biosynthetic Pathways
Kosuke Iwai1,2, Maren Wehrs3, Peter W. Kim1,2, Trent Northen1,4, Aindrila
Mukhopadhyay3,5, Hector Garcia-Martin3,5, Paul D. Adams1,6,7, and Anup K Singh1,8
(1)Technology Division, Joint BioEnergy Institute, Emeryville, CA, (2)Biotechnology and
Bioengineering Department, Sandia National Laboratories, Livermore, CA, (3)Biological
Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA,
(4)Environmental Genomics and Systems Biology Division, Lawrence Berkeley National
Laboratory, Berkeley, CA, (5)Biofuels and Bioproducts, Joint BioEnergy Institute,
Emeryville, CA, (6)Department of Bioengineering, University of California, Berkeley,
Berkeley, CA, (7)Molecular Biophysics and Integrated Bioimaging Division, Lawrence
Berkeley National Laboratory, Berkeley, CA, (8)Biological and Engineering Sciences,
Sandia National Laboratories, Livermore, CA
222. Engineering an Orthogonal DNA Replication System for Sexual
Recombination and Biosensor Evolution
Alex Javanpour Biomedical Engineering, UC Irvine, Irvine, CA
223. Design of a Layered Feedback Controller in Biological Systems
Chelsea Hu and Richard M. Murray
Division of Biology and Biological Engineering, California Institute of Technology,
Pasadena, CA
224. Synthetic Genetic Incompatibility; dCas9-Based Programmable
Transcription Activation for Invasive Species Biocontrol and Transgene
Biocontainment
Samuel Erickson1, Siba Das2, and Michael J. Smanski3
(1)BioTechnology Institute, University of Minnesota, Saint Paul, MN, (2)University of
Minnesota, Saint, MN, (3)Department of Biochemistry, Molecular Biology, and
Biophysics, University of Minnesota, Twin Cities, St. Paul, MN
225. Bioswitches and Robots for Systems Metabolic Engineering and Synthetic
Biology of Hyper Microbial Production Strains
Lifu Song1,2, Uwe Jandt2, Lin Chen2, Jie Ren1, Libang Zhou1, and An-Ping Zeng1,2
(1)Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing
University of Chemical Technology, Beijing, China, (2)Institute of Bioprocess and
Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany
226. Emerging Technologies and Frontiers of Biomolecular Engineering:
Combining Rational Design, Machine Learning and Automated Experimental
Feedback
Uwe Jandt1 and An-Ping Zeng1,2
(1)Institute of Bioprocess and Biosystems Engineering, Hamburg University of
Technology, Hamburg, Germany, (2)Beijing Advanced Innovation Center for Soft Matter
Science and Engineering, Beijing University of Chemical Technology, Beijing, China
227. Orthogonal Transmembrane Chemical Signalling across Synthetic Cells
Yujia Qing1 and Hagan Bayley2
(1)Chemistry, University of Oxford, OXFORD, United Kingdom, (2)Chemistry,
University of Oxford, Oxford, United Kingdom
228. Identification and Characterization of Arabinoxylanases from a Newly
Isolated Thermophilic Bacterium to Improve Fermentation of Corn Fiber
Dhananjay Beri1, Lee R. Lynd2, William S. York3, Maria Pena3, and Christopher D.
Herring4
(1)Thayer school of Engineering, Dartmouth College, Hanover, NH, (2)Thayer School of
Engineering, Department of Biological Sciences, Dartmouth College, Hanover, NH,
(3)Complex Carbohydrate Research Center, University of Georgia, Athens, GA, (4)Enchi
Corporation, Waltham, MA
229. Genome Mining and Strain Engineering to Identify Novel Drugs from
Filamentous Fungi
Nadia Chacko, Venkatesh Endalur Gopinarayan, Cindy Guo, Colin Lazzara, Paul
Secrist, Gregory Verdine, and Philippe Prochasson
LifeMine Therapeutics, Cambridge, MA
230. Pairing Genome Engineering of Synthetic Yeast Strains with High
Throughput Screening for Enhanced Flavonoid Production
Jamie Auxillos University of Edinburgh, Edinburgh, United Kingdom; University of Manchester,
Manchester, United Kingdom
231. Evaluation of Engineered Microbe Persistence and Function of Using a
Simplified Polymicrobial Gut Community
Steven Arcidiacono CCDC-Soldier Center, Natick, MA
232. Microbial Dynamic during Start-up Process of Anaerobic Digestion of
Sugarcane Vinasse
Licelly Canizalez-González1, Edgar Blanco2, John Ward3, and Maria F Villegas-Torres4
(1)Universidad Icesi, Cali, Colombia, (2)Anaero Technology, Cambridge, United
Kingdom, (3)Department of Biochemical Engineering, University College London,
London, United Kingdom, (4)Universidad Icesi, CALI, Colombia
233. Biotechnological Applications for Light-Regulated Cre Recombinase
Lena Hochrein1, Leslie A. Mitchell2, and Bernd Mueller-Roeber1
(1)Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany,
(2)Institute for Systems Genetics, NYU Langone Health, New York, NY
234. Engineered Microbes for Therapeutic Applications
Chun Loong Ho Department of Biomedical Engineering, Southern University of Science and Technology,
Shenzhen, China
235. Learning from Evolutionary Biology to Engineer Crop Plants for the Future
Prem Bhalla and Mohan Singh
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville,
Australia
236. Toward a Translationally-Independent Guide RNA Oscillator
James Kuo1,2, Ruoshi Yuan1, Johan Paulsson1, and Pamela A. Silver1,2
(1)Systems Biology, Harvard Medical School, Boston, MA, (2)Wyss Institute for
Biologically Inspired Engineering at Harvard University, Boston, MA