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Protocol
A vaccine will be created and distributed to persons at highest risk of contracting this
emerging deadly disease.
INTRODUCTION
This emerging deadly disease is causing brain abscesses in patients that contract it. In
order to create a drug to combat the symptoms of the disease, it would take years of
research and money that we, the research team, do not have. On average it takes 12 years
for a drug to make its way from the research lab to the patient (CBRA). Creating a cure is
not an effective way to combat this disease. The only way to protect citizens is disease
prevention. This can be done in multiple ways, such as preventing transmission by
breaking up the transmission cycle by eliminating one of the links that keeps the cycle
going. In order for a pathogen to persist it must be able to exit an infected host, survive
the environment during transmission, enter a new host, and multiply in the new host
(Goddard 2012). The two points in the transmission cycle that could possibly be targeted
is the vector and the reservoir. The vector is Ixodes pacificus, or commonly known as the
black legged tick (CAPC 2015). The elimination of this species of tick would take years
in order to complete because it is found in 90 counties in five different western states
(Dennis, Nekomomoto, Victor, Paul, and Piesman 1998). This widespread dispersion
prevents this target of control from being a feasible prevention technique. The next point
in the cycle that could be targeted is the reservoir. The reservoir is Aphelocoma
californica, or the western scrub jay. This Avian species can be found along the western
part of North America (Curry 2002). The widespread dispersion of the species would
make the task of elimination difficult, costly, and time consuming. This would not be an
efficient initial protocol for prevention of the disease. The only way to combat the disease
efficiently and effectively would be to create a vaccine that protects against the bacterial
agent, Lactococcus jimmi, and distribute the vaccine first to those at highest risk of
contracting the disease.
The main goal in the production and distribution of the vaccine is affordability. Keeping
costs down will give more people access to the vaccine and create a broader range of
coverage. A broad range of coverage is of utmost importance when dealing with this
emerging deadly disease because of the wide distribution of both the vector and reservoir.
Stopping the transmission cycle before it gets started will allow the disease to become
obsolete. The protocol given will provide the steps needed in order to obtain this goal.
PROTOCOL
Steps
1. Creation of Vaccine
The bacterial pathogen that is responsible for this emerging deadly disease is L.
jimmi. Vaccines that guard against bacterial pathogens are made in a number of
ways, such as identifying the bacterial toxin and making a toxoid, using the
polysaccharide of the bacteria, or using two or more proteins from the bacteria
(Children’s Hospital of Philadelphia). Any of the previous three routes of making
a vaccine would work in the production of the vaccine. In order to proceed
efficiently we have decided to utilize the polysaccharide coating in order to create
the vaccine. Polysaccharides and proteins add to the complex arrangement of the
peptidoglycan layer of gram-positive bacteria (Charpot-Chartier and Kulakauskas
2014). There are three groups of polysaccharides: exopolysaccharides, capsular
polysaccharides, and cell wall polysaccharides. Capsular polysaccharides form a
shield around the bacterium. Targeting these polysaccharides specifically in the
creation of our vaccine will allow us to disrupt the bacterium’s defenses and allow
phagocytosis to occur (Bentley, Aanensen, Mavroidi, Saunders, Rabbinowitsch,
Collins, Donhoe, Harris, Murphy, Quail, Samuel, Skovsted, Kaltoft, Barrell,
Reeves, Parkhill, and Spraft 2006).
2. Distribution of Vaccine
Distribution of the vaccine will be based off of three things: age distribution,
geographic location of residence, and lifestyle.
Age Distribution: The age distribution for vaccination is mainly 21 years of age to
70 years of age with mild precautions for those above 70 years of age. In other
words children are not our main focus in the distribution of the vaccine. This
information is based off the age distribution of patients that have been reported as
having contracted the disease.
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Age Range
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Geographic Location of Residence: The vaccine will be distributed to those who
reside along the Pacific coast in the Western United States. The states included in
this description are Washington, Oregon, and California. The major counties of
concern are San Luis Obispo County, Humboldt County, San Francisco County,
Los Angeles County, and Santa Barbara County in California and Thurston
County in Washington. These counties were identified as the western scrub jay’s
hotspots.
Lifestyle: The vaccine will be distributed to individuals who have an occupation
or hobby that places them in areas where the vector, I. pacificus can be found.
This includes forested or brushy areas, north coastal scrub, and open grasslands
(Slowik and Lane 2001). An example of an occupation that increases the
likelihood of contracting the disease is farming. Hobbies include jogging, bird
watching, and hiking.
Individuals of highest concern are those that fit all three of the previously listed
criteria: 21 to 70 years of age, live in Thurston, San Louis Obispo, Humboldt, San
Francisco, Los Angeles, or Santa Barbara County, and partake in outdoor
lifestyles.
Materials and Methods
Materials: There are approximately 5,255,112 individuals living in the hotspot counties
of Thurston, San Louis Obispo, Humboldt, San Francisco, Los Angeles, and Santa
Barbara (US Census Bureau). The percent of people in these counties that are in the age
range from 21 to 70 is approximately 64% (Wikipedia). The percent of people in the
United States that participate in some form of outdoors activity is 49.4% (The Outdoor
Foundation 2013). The total number of single dose vaccine vials needed is approximately
1,700,000. This number is based off the total population in the hotspot counties
(5,255,112) times the percent of people that fall in the required age group (64%) times
the percent of people who participate in some form of outdoor recreation (49.4%). The
same number (1,700,000) of 3 mL 1.5 inch needle syringes will be needed.
Methods: The vaccine will be created using the polysaccharide coating as the basis of the
vaccination. The vials will then be filled with the vaccine and distributed. Distribution
will take place at local clinics and hospitals in the six major counties: San Luis Obispo
County, Humboldt County, San Francisco County, Los Angeles County, and Santa
Barbara County in California and Thurston County in Washington. Television
commercials, radio announcements, and flyers will be used to spread the word about the
impeding threat of the emerging deadly disease and the vaccine as a solution. The
vaccine will be distributed in three phases. Phase one will distribute vaccines to only the
individuals who meet the criteria: 21 to 70 years of age, live in Thurston, San Louis
Obispo, Humboldt, San Francisco, Los Angeles, or Santa Barbara County, and partake in
outdoor lifestyles. Phase one will last approximately one week then phase two will begin.
Phase two will distribute vaccinations people of all ages living in the six major counties
and partaking in outdoor lifestyles. Phase two will last approximately one week then
phase three will begin. Phase three will allow anyone, regardless of age, geographic
location, and lifestyle, to obtain the vaccination.
Phase 121 to 70 years of age, live in Thurston, San Louis Obispo, Humboldt, San Francisco,
Los Angeles, or Santa Barbara County, and partake in outdoor lifestyles
Phase 2 People of all ages living in the six major counties and partaking in outdoor lifestyles
Phase 3 Anyone (regardless of age, geographic location, and lifestyle)
CONCLUSION
The most plausible way to protect humans from the bacteria L. jimmi, is by creating a
vaccine that will break the cycle between the bacterial agent L. jimmi and humans.
Vaccinations will boost the patient’s immune system by creating a set of antibodies that
recognize the polysaccharide coat of L. jimmi.
The vaccination will be distributed primarily in locations where the reservoir, the western
scrub jay resides, in the western portion of the United States, along the pacific coast. L.
jimmi are able to complete their life cycle and increase their numbers inside the western
scrub jay. Where ever the western scrub jay goes the increased risk of exposure to L.
jimmi also increases. Sending vaccinations to locations where the western scrub
jay primarily resides will decrease the rate of infection amongst individuals who reside in
the same geographical area.
People who are urged to receive the vaccine are those who primarily spend their time
outdoors in areas where they can be exposed to the vector I. pacificus. I. pacificus passes
on L. jimmi to humans when they are in the process of completing their blood meal. Since
individuals will be vaccinated and have a set of antibodies against the bacteria, they will
not have to be weary when they are outdoors working or just enjoying the day outdoors.
According to the U.S Department of Health and Human Services, we can ensure that our
vaccine has been successful by performing an antibody test. However, this can be
expensive to test every patient who has received the vaccine (U.S. Department of Health
and Human Services). Instead, we will have hospitals report new cases of our disease. If
any new case develops in someone that has been previously vaccinated an antibody test
will be run.
If the steps are followed precisely, as defined in the protocol, then we should be able to
eliminate the possibility of infection of L. jimmi. Therefore stopping the spread of this
deadly infectious disease and save lives.
REFERENCES
Bentley SD, Aanensen DM, Mavroidi A, Saunders D, Rabbinowitsch E, Collins M, Donohoe K, Harris D, Murphy L, Quail MA, Samuel G, Skovsted IC, Kaltoft MS, Barrell B, Reeves PR, Parkhill J, Spratt BG. 2006. Genetic Analysis of the Capsular Biosynthetic Locus from all 90 Pneumococcal Serotypes. PLoS Genet. 10.1371/journal.pgen.0020031. Web.
(CAPC) Companion Animal Parasite Council. 2015. Vector-Borne Diseases – Ixodes Scapularis and Ixodes Pacificus. <https://www.capcvet.org/capc-recommendations/ixodes-scapularis-and-ixodes-pacificus>.
(CBRA) California Biomedical Research Association. Fact Sheet New Drug
Development Process.
www.ca-biomed.org/pdf/media-kit/fact-sheets/CBRADrugDevelop.pdf. Web.
Chapot-Chartier, M-P and S. Kulakauskas. 2014. Cell Wall Structure and Function of
Lactic Acid Bacteria. Microb Cell Fact. 10.1186/1475-2859-13-S1-S9. Web.
Children’s Hospital of Philadelphia. Making Vaccines: How are Vaccines Made?.
http://www.chop.edu/centers-programs/vaccine-education-center/making-vaccines/how-
are-vaccines-made.
Curry, R. 2002. Western Scrub-jay: Aphelocoma californica. Phiadelphia, Pa.: Birds of North America.
Dennis, D. T., T. S. Nekomoto, J. C. Victor, W. S. Paul, and J. Piesman. 1998. Forum: Reported Distribution of Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae) in the United States. J Med Entomol. 35(5):629-638. Web.
Goddard J. 2012. Public Health Entomology. 1439848823, 9781439848821.
The Outdoor Foundation. 2013. Outdoor Participation Report. www.outdoorfoundation.org/pdf/ResearchParticipation2013.pdf.
Slowik, T. J. and R. S. Lane. 2001. Nymphs of the Western Black-legged tick (Ixodes pacificus) Collected from Tree Trunks in Woodland-grass Habitat. J Vector Ecol. 26(2): 165-171.
U. S. Census Bureau. 2013. Population Estimates.
http://www.census.gov/popest/about/terms.html.
U.S. Department of Health and Human Services. The effectiveness of
immunizations. http://archive.hhs.gov/nvpo/concepts/intro6.htm.
Wikipedia. Demography of the United States.
https://en.wikipedia.org/wiki/Demography_of_the_United_States.