Embryology Doc. MUDr. Ing. RNDr. Peter Celec, DrSc., MPH [email protected] .
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Transcript of Embryology Doc. MUDr. Ing. RNDr. Peter Celec, DrSc., MPH [email protected] .
Stem cells
• Totipotency• All tissue types
• Pluripotency• All tissues except placentaand amniotic sac
• Multipotency• Several related tissue types
Differentiation
Programming and reprogramming
Nucleus transfer
• John Bertrand Gurdon (1933 - )• Nobel prize in Physiology or medicine 2012
Somatic cell nuclear transfer
• Dolly(5 July 1996 – 14 February 2003)
Cloning
• Reproductive • Therapeutic
Cloning
Pluripotency
iPSC
Shinya YamanakaKyoto University
iPS
• Reprogramming of differentiated cells• Pluripotency• No immune response
• Without retroviral vectors• Without oncogenes• pH?
• Reproducibility
Shinya Yamanaka(1962 – )Nobel prize2012 – Physiology or medicine
Malignant transformation
• Robert Weinberg (1942 - )
• Oncogenes• Ras
• Tumor suppressors• Rb1
Hallmarks of cancer
Hanahan & Weinberg, Cell, 2000
Hallmarks of cancer
Hanahan & Weinberg, Cell, 2011
Malignant transformation
• Bert Vogelstein (1949- )
• Vogelgram
Genometastasis
Genometastasis
www.imbm.sk