ELITE™ Custom Antibody Services

ELITE™ Custom Antibody Services … Experience, confidence, and understanding

As a manufacturer and service provider, we have the experience, confidence, and understanding to develop an antibody that meets your specific requirements. From our polyclonal antibody services through high throughput clone screening, let us put our expertise to work for you.

Antigen selection and design

Peptide purification & conjugation

Immunization Primary Screen

Delivery Validation & optimization

Antibody purification

Secondary screen

Overview of Antibody Development Workflow

Antigen selection and design Abgent has vast experience with antigen selection and design – we’ll work with you to develop the antigen best for your antibody target and application/s. Compounds

Recombinant proteins

Custom designed peptides

Whole cells

Immunization Whether it’s our improved immunization process (below) or your customized immunization protocol, we’ll work with you to reduce project time and improve project success rate.

A novel approach to adjuvant preparation and immunization Current immunization protocols involve a greater than two month process, tedious adjuvant preparation, undue stress on the animals, and a relatively low success rate. Compared with the conventional Freund’s adjuvant, our new procedure allows for fewer immunizations, a reduced antigen dosage, and quick antibody induction. Resulting antibodies show a high titer and high affinity. Our new process does not alter the antigen conformation and is easy to use.

Primary Screening: High capacity ELISA High capacity flow cytometry

Secondary screening: Western Blot Immunofluorescence Immunohistochemistry Functional assay Customer’s preferred assay

Screening We’ll work to screen, identify, and validate rare clones. Our project team will suggest the screening process best for your specific antibody requirements. We have 300 cell lines and abundant cynomolgus and human tissues.

Validation and optimization

Immunofluorescence

Immunohistochemistry

Flow cytometry

Immunoprecipitation

Antibody labeling

Scale up

With over a decade of experience developing 20,000 antibodies for research and drug discovery, we understand your application/s requirements. We’ll deliver a validated antibody optimized for your specific application/s.

Validation and optimization Our validation approach has the highest Quality Standard. By choosing proper cell lines and tissues, and applying correct experimental design we assure that your antibody has biological and functional significance

LC3 Antibody (APG8B) (N-term) TTR Antibody (C-term)

Basic ELITE™ Custom Service Packages Abgent offers basic packages for monoclonal and polyclonal antibody development . These packages optimize project delivery time by offering limited customization. More complex customization is available based upon you specific needs.

Modification-state Antibodies We’ll work with you, from antigen design through scale up, to deliver antibodies validated and optimized for your application/s of interest.

Phosphorylation plays a critical role in signaling cascade Many oncology drug discovery targets are known to be active/inactive upon phosphorylation event

Methylation and acetylation events are essential steps in epigenetic gene activation/silencing. These modifications are increasing in importance within drug discovery

ELITE™ Custom Services for Drug Discovery We understand the diversity of your antibody needs. Because of this, we offer an industry leading range of custom antibody services.

HTS ELISA screen

Flow cytometry based high throughput phenotypic screening

Biochemical characterization of antibody

Recombinant antibody production

Cell line generation and cell based assays for validation

Sandwich ELISA antibody pairing

Antibody sequencing

In case that you may require scale or further antibody optimization, we suggest recombinant monoclonal antibody production. We have had great success developing recombinant antibodies to allow for scale, consistency, and quality.

mRNA isolation, cDNA and gene synthesis

Transfection to CHO or 293 cells

Recombinant antibody purification

Functionality assessment

ELITE™ Services for Drug Discovery Recombinant Antibody Production Platform

Marker Supernatants Purified Herceptin

kD

1508058

45

30

25

1 2 5 10 μg

Reduced 15% SDS-PAGE of Herceptin

Heavy chain

Light chain

Marker Supernatants Purified Herceptin

kD

1508058

45

30

25

1 2 5 10 μg

Marker Supernatants Purified Herceptin

kD

1508058

45

30

25

1 2 5 10 μg

Reduced 15% SDS-PAGE of Herceptin

Heavy chain

Light chain

ELITE™ Services for Drug Discovery

To increase project success by identifying rare clones, we offer high-throughput clone screening using ELISA with a primary screening capability of 10,000 to 50,000 samples per assay.

High Capacity ELISA Clone Screening Platform

The Intellicyt™ Screener – a revolution in screening in solution. Flow-cytometry based, high-throughput, phenotypic screening of suspension cells can now be performed by Abgent. For antibody discovery studies, the ability to acquire both antigen binding and cross-reactivity simultaneously allows for the critical collection of data. Moreover, targets can now be screened in the context of the cell.

ELITE™ Services for Drug Discovery High Capacity Flow-based Screening Platform

ELITE™ Services for Drug Discovery FACS (Fluorescence-activated cell sorting ) and MACS (Magnetic-Activated Cell Sorting ) services

Measure cell surface markers by FACS

Cell sorting by MACS Cell based assays

B

B

B

B

Splenocyte

Resting B cell MACS

ELITE™ Services for Drug Discovery HTS cell-based & beads-based assay by Intellicyte

Cell based assays : compound screening, compound characterization, and toxicology evaluation

Bead based assays : compound screening and phamaco-dynamic assessment

High throughput and Multiplex

ELITE™ Services for Drug Discovery The Acumen Explorer microplate cytometer offers researchers a flexible, high-throughput, high-content screening approach for many biochemical screens.

Cell cycle analysis by Acumen

Acumen eX3

Cell line generation and cell based assays for validation

ELISA antibody pairing

Antibody sequencing

Biochemical characterization of antibody

ELITE™ Services for Drug Discovery – additional services

Surface Plasmon Resonance

ChIP

How ELITE Service Fits into Drug Discovery Pipeline

Anywhere an RUO custom antibody is needed in the drug discovery and development process or companion diagnostics

Summary of Applications Areas Where ELITE™ Custom Services Can Be Applied

Conclusion Experience, Confidence and Understanding

Unsurpassed hybridoma screening capabilities allows for identification of rare clones and increased project success rate. Comprehensive validation and optimization in customer-defined applications ensures that the antibody will work the first and each time used in your specific application. 300 ATCC cell lines; abundant mouse, rat, rabbit, cynomolgus and human tissues.

Rapid turnaround time leveraging our experience developing over 20,000 antibodies. Project support from industry veterans ensures rapid and concise communication.

Abgent’s animal facilities with recognized AAALAC credentials.

THANK YOU FOR ATTENTION!