Cartilage TE Strategies

J o a n a M a g a l h ã e s , P h D

U n i d a d d e B i o i n g e n i e r í a T i s u l a r y T e r a p i a C e l u l a rG r u p o d e R e u m a t o l o g í a

I n s t i t u t o d e I n v e s t i g a c i ó n B i o m é d i c a d e A C o r u ñ a ( I N I B I C )

G B T T C - C H U A CC e n t r o d e I n v e s t i g a c i ó n B i o m é d i c a e n R e d e n B i o i n g e n i e r í a ,

B i o m a t e r i a l e s y N a n o t e c n o l o g í a ( C I B E R - B B N )

Current strategies for cartilage regeneration

Vinatier et al. Trends in Biotech. 2009

ACI

Cell-based

2008

Diekman et al. CO Rheumatol. 2012

Scaffold-based

Hutmacher et al. Macromol. Biosci. 2009

Current strategies for cartilage regeneration

Correia et al. Adv Exp Med Biol 2015Diekman et al. CO Rheumatol. 2012

TGF-β3-PEPM-A-HA functionalized gels for cartilage TE

Magalhães et al. J Bioact Comp Polym 2014

TGF-β3-PEPM-A-HA functionalized gels for cartilage TE

TGF-β3-PEPM-

A-HAAldehydeA-HA =

Schiff’s-base crosslinking method

Release systems: burst and controlled (zero-order).

HA =

TGF-β3 =

Dry state

PEPM-HA

TGF-β3 =

Proliferation: positive effect on BM-MSCs

PLLA-CHT scaffolds for cartilage TE

Magalhães et al. T Eng Part A, 2015

Scaffold morphology and composition influence chondrogenic differentiation of MSCs. Fiber-coating induces a more stable phenotype.

PLLA-CHT scaffolds for cartilage TE

PLLA PLLA-CHT1 PLLA-CHT2

7 days

14 days

7 days

14 days

30 days

Magalhães et al. World Biomed Front, 2015

PLLA-CHT scaffolds for cartilage TE

Magalhães et al. World Biomed Front, 2015

Conclusions

Regenerative medicine approaches offer great promise toachieve regeneration of hyaline cartilage.

We have successfully fabricated a controlled release system ofTGF-β3 to induce the chondrogenic differentiation of MSCs.

Morphological and compositional manipulation of PLLA-CHTinfluences the zonal architecture components of neocartilagetissue formed.

GBTTC

Dr. Francisco J. Blanco

Rheumatology Group

INIBIC-Hospital Universitario de A Coruña

Joana Magalhães

Elena F. Burguera

Tamara Hermida

Lucía Gato

Mª Cristina Álvarez

Cristina Rodríguez

Noemí Fernández

Purificación Filgueira

Noa Goyanes

Estefanía López Senra (Visitor Scientist)

Colaboradores

J San Román, CSICP Hatton, A Crawford U SheffieldR Reis, J Mano, 3B’s, UMR Amandi, STEMMATERSL Rodríguez Lorenzo, CSICL Rojo, King's College LondonJL Gómez Ribelles, UPVJ Samitier, IBECP González, J Serra, U. VigoD Simes, UAlgv

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