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Before the advent of the Edman degradation, the primarystructures of proteins were elucidated through the use of partialacid hydrolysis. The resulting oligopeptides were separated andtheir amino acid compositions were determined. Consider apolypeptide with amino acid composition (Ala2, Asp, Cys, Leu,Lys, Phe, Pro, Ser2, Trp2). Treatment with carboxypeptidase A released only Leu. Oligopeptides with the following compositions were obtained by partial acid hydrolysis:(Ala, Lys) (Ala, Ser2) (Cys, Leu)(Ala, Lys,Trp) (Ala,Trp) (Cys, Leu, Pro)(Ala, Pro) (Asp, Lys, Phe) (Phe, Ser,Trp)(Ala, Pro, Ser) (Asp, Phe) (Ser,Trp)(Ser2,Trp)

Determine the amino acid sequence of the polypeptide.1

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