Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing...

Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing After ACL

Reconstruction in a Rat Model

by Robert H. Brophy, David Kovacevic, Carl W. Imhauser, Mark Stasiak, Asheesh Bedi, Alice J.S. Fox, Xiang-Hua Deng, and Scott A. Rodeo

J Bone Joint Surg AmVolume 93(4):381-393

February 16, 2011

©2011 by The Journal of Bone and Joint Surgery, Inc.

Example of mounted external fixator on specimen with Keith needle in place.

Robert H. Brophy et al. J Bone Joint Surg Am 2011;93:381-393


Example of animal connected to loading device.



Example of negative control histology (mouse anti-rat ED1– microphage stain; original magnification, ×20).



ED1 staining (mouse anti-rat ED1-macrophage stain) showing inflammatory macrophages in the tissue of a rat from the immobilized group killed at two weeks (original magnification, ×20).



Factor VIII staining (rabbit anti-human factor VIII, DAKO) showing endothelial cells in the tissue of a rat from the immobilized group killed at four weeks (original magnification, ×20).



Procollagen staining (Santa Cruz Biotechnology) revealing collagen synthesis in the tissue of a rat from the immobilized group killed at four weeks (original magnification, ×20).



Graph showing the mean daily loading curve (and standard deviation) of the rats that underwent daily loading for two weeks or four weeks.



Graph showing the mean stiffness data (and standard deviation) from isolated bone-tendon-bone specimens.



Graph showing the mean load-to-failure data (and standard deviation) from isolated bone-tendon-bone specimens.



Effect of Short-Duration Low-Magnitude Cyclic Loading Versus Immobilization on Tendon-Bone Healing...

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