EFFECT OF GENOTYPES ON MICROPROPAGATION OF … · history and background jatropha curcas is a...
Transcript of EFFECT OF GENOTYPES ON MICROPROPAGATION OF … · history and background jatropha curcas is a...
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U L Y S I U S M C G H E E
T H E F O R T V A L L E Y S T A T E U N I V E R S I T Y
N S F - R E U , T H E P E N N S Y L V A N I A S T A T E U N I V E R S I T Y
EFFECT OF GENOTYPES ON MICROPROPAGATION OF
JATROPHA CURCAS
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IMPERIAL JOURNAL OF PHARMACONOSY & NATURAL PRODUCTS
PLANT TISSUE CULTURE OF JATROPHA CURCAS L.: A REVIEW
PREET I GARG, PANKAJ KHATR I , D IVAY GANDHI
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Kingdom: Plantae
Order: Malpighiales
Family: Euphorbiaceae
Genus: Jatropha
Species: curcas
PHYLOGENY: JATROPHA CURCAS
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PLANT TISSUE CULTURE (INTRODUCTION)
-a technique of growing tissue under a suitable
environment that promotes successful growth
• Clone
• Disease free plants
• Production of large number of plantlets
• Germplasm maintenance
• Regeneration of whole plant
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TECHNIQUES
• Sterilization of explants
• Solid medium
• Liquid medium
• Hormones
• Callus and cell culture
• Shoot culture
• Regeneration from shoot explant
• Agrobacterium mediated transformation
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• Oil
• Edible
• Fish poison
• Soaps
• Illumination
USES OF J. CURCAS
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USES OF JATROPHA CURCAS (CONT)
Plant part used Diseases
Seeds To treat arthritis, gout and jaundice
Tender twig/ stem Toothache, gum inflammation, gum bleeding, pyorrhea
Plant sap Dermatomucosal diseases
Plant extract Allergies, burns, cuts and wounds, inflammation, leprosy, leucoderma, scabies and small pox, wound healing
Water extract of branches HIV, tumor
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STEM EXPLANTS
• Simple, rapid and
cost effective
protocol
• Shoots cultured on
medium containing
IBA
• 40% rooting
achieved after 5
weeks
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HISTORY AND BACKGROUND JATROPHA CURCAS IS A POISONOUS, TOXIC, DROUGHT RESISTANT PLANT THAT IS
CULTIVATED IN TROPICAL AND SUBTROPICAL REGIONS. TODAY, J. CURCAS IS A WIDELY USED AS A BIOFUEL CROP.
• Physic nut
• Seed contains about 30-50% of oil
• Seed contains taxalbumin curcin
• Genome sequenced in October, 2010
• Life expectancy 40 years
• Biodiesel (major use)
The genus name Jatropha derives from the Greek jatros (doctor), trophe (food)
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MEDIA PREPARATION
Two media used
1. Jatropha shoot medium (MS + Vitamin+ 30g/l Sucrose + 3 mg/l BAP
+ 0.1 mg/l IBA + pH 5.8 + Agar 7 g/l) sterilized at 121˚C, 20 minutes
2. Callus Induction medium (MS + Vitamin + 30g/l Sucrose + 1.5 mg/l BAP
+ 0.05 mg/l IBA + pH 5.8 + Agar g/l) sterilized at 121˚C for 15”
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DECONTAMINATION
• Wash (Tween20) 10’
• Hood
• 10% bleach (5’)
• Sterile water (3x)
• .1% Mercuric
Chloride (5’)
• Sterile water (5-6x)
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PRELIMINARY RESULTS
• Rate of contamination
• Early response (axillary bud break, curling response)
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PRELIMINARY RESULTS (CONT.)
Total Percent Contaminated: 16.36%
Percent Contamination
GT1: 9.09%
GT2: 33.33%
GT3: 12.50%
GT12: 12.50%
GT5: 6.25%
GT9: 6.25%
Total Percent Contaminated: 0%
Percent Contamination
GT1: 0%
GT2: 0%
GT3: 0%
GT12: 0%
GT5: 0%
GT9: 10%
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CONCLUSION
• There is no clear difference between the 10
genotypes Tested so far. It is early to make a
conclusion but will have a better understanding by
the end of this REU program.