Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
-
Upload
hesham-n-mustafa -
Category
Documents
-
view
8 -
download
0
description
Transcript of Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
1/36
Effect of
Acrylamide
on
Stomach, Cerebellum
and Testis of the Albino
Rat
By
Hesham
Noaman
Abdel
Raheem
M.D. THESIS
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
2/36
Acknowledgement
should go to all persons who have
assisted, in a way or another, to allow
this study to come to light;
Doctor:
Kariman
Mohammed El
-
Gohari
(Professor of Anatomy and Head of
Anatomy Department).
Doctor: Hassan
Mostafa
Serry
(Professorof Anatomy).
Doctor:
Shahira
Samir
Zaki
(Assistant
Professor of Anatomy).
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
3/36
Introduction
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
4/36
Acrylamide
Chemistry
Uses
CH2=CHCONH2
water treatment,
enhanced oil recovery,
papermaking aids,
soil conditioning agents,
sewage and waste treatment,
ore processing,
permanent
-
press fabrics.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
5/36
Human Exposure to
Acrylamide
Acrylamide
is a chemical compound formed de
novo when certain carbohydrate
-
rich foods fried,
baked, or roasted at high temperatures.
It was not known to be in food until
April
24
,
2002
,
where
Swedish scientists reported high
concentrations of in fried, baked foods.
Maillard
reaction
is reaction of reducing sugar
(as glucose) with free
asparagine
, an amino acid
found in many foods, especially at high
temperature.
WHO/FAO: Dietary intake:
0.3
-
0.8
g/kg
BW/day.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
6/36
0
5
10
1520
25
30
ug/day
Biscuit / Cookies
All Other Foods
Exposure to
Acrylamide
in Food
French Fries &
Potatoes
Breads
Potato Chips
Cereal
Coffee
Cakes
Dried Foods
Pop Corn
Salty Snacks
Chocolate
Nuts/Seeds/
Butters
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
7/36
Effect of temperature
Acrylamide levels in potato chips friedfor 4 minutes increased with frying
oil temperature. (ppb=parts per billion)
160 C
27ppb
170 C
70ppb
180 C
326ppb
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
8/36
Effect of time
Acrylamide levels in potato chips fried at 180oCincreased with frying time.
3.5 m
12ppb
4 m
46ppb
4.5 m
227ppb
5 m
973ppb
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
9/36
Aim of thework
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
10/36
The current project aimed at exploring the harmfuleffects of acrylamide on the structure of the stomach,
cerebellum and testis in the albino rat, in an attempt to
clarify its potential risk on the human health.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
11/36
Material and
Methods
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
12/36
The stomach, testis and cerebellum specimens
were collected form fifty adult male albino rats.
Animals were divided to two main groups: I, IIeach group was subdivided into 3 subgroups.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
13/36
Specimens from testis, cerebellum and stomach were
extracted and processed for light and electron
microscopic examination. For the light microscope serial
sections obtained, stained with H&E (Testis, Cerebellum
and Stomach); Feulgen stain (Testis) and silver stain(Modified Glees) (Cerebellum). An image analyzer was
used to assess cellular apoptosis, and in addition, DNA
cytometrywas performed.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
14/36
Results
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
15/36
I- Effect of Acrylamide on testis
Animals that received 25mg/kg BW/10daysshowed mild affection.
Animals that received 50mg/kg BW/10days
showed evident damage especially with theintraperitoneal administration. This was in the
form of degeneration of germ cells, numerous
multinucleated giant cells with sloughed
seminiferous epithelium, and vacuolations in-between the germ cells.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
16/36
Control 50mg/kg/10days/ I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
17/36
Control
50mg/kg/10days/ I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
18/36
Control 50mg/kg/10days/ I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
19/36
Control 50mg/kg/10days/ I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
20/36
Control
50mg/kg/10days/ I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
21/36
50mg/kg/10days/ I.P. 50mg/kg/10days/ P.O.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
22/36
ApoptosisFeulgen stain was performed to assess the effect
of acrylamide on cellular apoptosis in the testis.It was clearly proved that acrylamide at a dose of
50mg/kg/10days showed increased apoptosis.
Control group 5/10
25mg/kg/10days whether orally or
intraperitoneally6/10
50mg/kg/10days/orally 9/10
50mg/kg/10days/intraperitoneally 15/10
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
23/36
St d f DNA C t t
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
24/36
Control 25mg/kg/10days whether orally or intraperitoneally
50mg/kg/10days/orally 50mg/kg/10days/ intraperitoneally
Study of DNA Cytometry
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
25/36
II- Effect of Acrylamide on
Cerebellum The Purkinje cells were target cells to acrylamide. Rats treated at a dose of 25mg/kg/10days showed
that Purkinje cells somata appeared unaffected, whiledegeneration of their dendrites and axons were evidentin the molecular and white matter respectively.
In addition, Rats treated with 50mg/kg/10days
showed argyrophilic degenerated Purkinje cells somata.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
26/36
Control
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
27/36
25mg/kg/10days/Orally
25mg/kg/10days/I.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
28/36
50mg/kg/10days/ I.P.50mg/kg/10days/Orally
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
29/36
Control
25mg/kg/10days/I.P.
Control
50mg/kg/10days/P.O.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
30/36
III- Effect of Acrylamide on
StomachThe stomach of animals treated with acrylamide
in a dose of 25mg/kg/10days showed no gastric
affection, while there were mild degenerative
changes and an apparent increase in mucous
secreting cells in the group that received
50mg/kg/10days.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
31/36
50mg/kg/10days/IP
Control
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
32/36
Control
50mg/kg/10days/.P.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
33/36
50mg/kg/10days/I.P.Control
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
34/36
Conclusion
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
35/36
The present study expands the available information
concerning the hazards carried by the consumption
of acrylamide on testis, cerebellum and stomach. Although the doses of acrylamide utilized in the
present investigation were higher than the average
dietary daily intake in humans, 0.4-5 g/kg body
weight/day,yet the cumulative effects of suchtoxicant on human health are still waiting to be fully
identified.
Further studies focusing on the influence of
acrylamide on different organs in smaller doses forprolonged periods could aid in the full
understanding of hazards implicated by acrylamide.
-
7/17/2019 Effect of Acrylamide on Stomach, Cerebellum and Testis of the Albino Rat (PPT) 3.12.2008
36/36