Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirchneriella subcapitata
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7/16/2019 Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirchneriella subcapitata http://slidepdf.com/reader/full/ecotoxicological-bioassays-for-aquatic-ecosystems-with-microalgae-pseudokirchneriella 1/1 Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirch neriella sub capitata *Ronald Huarachi Olivera 1 ; Ursulo Yapo 2 ; Alex Dueñas 2 ; Gustavo Mendoza 2 ; Werner Silva 2 ; Giovani Alatrista 2 ; & Rosaura Gonzalez 2 1 Magister Program in Biotechnology, University of Antofagasta, Antofagasta, Chile. E-mail: [email protected] Web: http://www.researchgate.net/profile/Ronald_Olivera 2 Aquatic BiologyLaboratory, Academic Department of Biology, National University of San Agustin, Arequipa, Peru, Av. Alcides Carrión s/n Introduction & Study Objectives Results and Discussions Conclusions Microalgae Pseudokirchneriella subcapitata Pseudokirchneriella subcapitata Olav Skulberg (1959) river Nidelva, Akershus, Noruega Bioassay ecotoxicological Isolated Growth inhibition Effectiva Concentration EC 50 E5=Camaná Sampling points Effective Concentration(EC%) to 72 hours EC5 EC10 EC15 EC20 EC25 EC50 TU50 C.T. E1 23.9 7 36.31 48.05 60.04 72.68 157.14 0.64 5 E2 2.59 5.59 9.39 14.18 20.2 84.2 1.19 4 E3 27.3 32.54 36.66 40.3 43.72 60.7 1.65 3 E4 14.6 19.72 24.2 28.47 32.72 57.43 1.74 3 E5 89.1 93.88 96.99 99.39 >100 109.57 0.91 5 C.T.:Categories toxicological; 5:Non toxic; 4:slightly toxic;3:Moderately toxic. Growth Inhibition of Pseudokirch neriella subc apitata as areas of the watershed Camana - Majes 72 hours Watershed Camana Majes Southern Peruvian Arequipa Cusco Puno Activities Economic Agricultural-livestock Mining Fishery The aim of this study was to estimate ecotoxicological bioassays quick and simple in river systems as Taparza, Grande and Camana rivers - Majes (Arequipa, Peruvian) by the median Effective Concentration (EC 50 ) ca te gor is es as t he r ange of t oxi cit y and i nhi bi ti on growth Pseudokirchneriella subcapitata compared to physicochemical analysis that exceed the values of the Peruvian National Environmental Quality Standards (E QS ) a nd gu id el ines for wa te r qua lit y o f th e Wor ld Health Organization (WHO) Parameter Analysis Unit E1 E2 E3 E4 E5 EQS Category 3 EQS Category 4 WHO Irrigating with long stems and low animals drink Conservation aquatic environment Coliform Laboratory NMP/100mL - 490 79 7900 49 1000 1000 2000 n.a. Al Laboratory mg/L 0.55 0.02 18.05 19.64 1.68 5 5 - 0.2 Mn Laboratory mg/L 0.0202 0.042 0.743a 0.814a 0.153a 0.2 0.2 - 0.1 Fe Laboratory mg/L 0.382 0.026 15.12a 19.51ª 1.893a 1 1 - n.a STS Laboratory mg/L 24.94 <3 1315a 149ª 114a - - ≤25-100 n.a Parameters that exceed the EQS and WHO E3= Majes1 E4=Majes2 E2=Grande E1=Taparza 24 hours 48 hours Ecotoxicological bioassays with the microalga Pseudokirchneriella subcapitata in watershed Camana, Majes from region Arequipa, P eruv ia n s ho we d h ig he r inh ib it io n o f g ro wt h a nd low g ro wt h rat e in t he E 3 ( Ma je s1 ) a nd E 4 ( Ma je s2 ) s ta tion s w it h a m ed ia n E ff ec tive C on ce nt ra tion ( EC 50 ): E3 (EC 50 = 60.7%) and E4 (EC 50 = 57.43 %) categorizing them as moderately toxic. This inhibition may be associated with concentrations thermotolerant coliform, Al, Mn ,Fe and total dissolved solids in the E3 and E4 stations that exceed national standards Peruvian water quality(EQS) and the guidelines World Health Organization (WHO) . Growth rate of P. sub capitata as areas from the waterwhed C am an a - M aj es upto 72 hours a bioassay. Graphs showing the concentration-response effects bioassays sampling poi nts wi th gro wth Pseudokirchneriella s ubcapitata w it h E ff ec ti ve Concentration (EC) at different percentages to 72 hours. Our study has two important results. First, the intensity of the most polluted spots in the river systems by inhibiting growth of P. subcapitata compared to Peruvian Environmental Quality Standards (EQS) and water quality guidelines of the World Health Organization (WHO). Second, growth of P. subcapitata the median effective concentration (EC 50 ) and Toxicity Units (TU) at 72 hours bioassays sampling points compared to toxicological categories established by Bulich (1982) and Coleman & Quereshi (1985) . Counting in Neubauer chamber Microscopic view of microalgae P. subcap itata (100X) - Bulich A. A practical and reliable method for monitoring the toxicity of aquatic samples. Process Biochem. 1982; 17: 45-47.. - Coleman RN, Qureshi AA. Microtox and Spirillum volutans test for assessing toxicity of environmental samples. Bull. Environ. Contam. Toxicol. 1985; 35: 443-451B Bibliography STUDY AREA Pseudokirchneriella subcapitata strain was obtained from CENMA (Centro Nacional del Medio Ambiente) Universidad de Chile, Región Metropolitana, Santiago, Chile. Cultivated in 250 mL Erlenmeyer flasks with 100 mL culture medium CULTURE CONDITIONS Tº=24 - 25ºC BIOASSAY ECOTOXICOLOGICALWITH P. subcap itata - Negative Control (SolutionBuffer, 15 mg NaHCO 3 ) - 6,25% - 12,5% - 50% - Positive Control (Water problem sample) EFFECTIVE CONCENTRATION (EC 50 ) -Toxicity Units = TU TU= (EC 50 ) -1 x 100 µ = lnN – lnNo Δt Where: N = cell density at the end of trial No = nominal initial cell density ΔT = time interval considered Toxicological categories aqueous samples according to the results obtained using the ecotoxicological bioas says Pseudokirchneriella subcapitata (Bulich 1982; Coleman & Quershi 1985) EC 50 To xi ci ty C at eg or y R an k To xi ci ty Un it s( TU ) < 25 Very Toxic 1 >4 25 - 50 Toxic 2 2 - 3.99 51 - 75 Moderately toxic 3 1.33 - 1.99 76 - 100 Slightly toxic 4 1.01-1.32 >100 NonToxic 5 < 1 - E1= Taparza (791888 UTM East, 8333378 UTM North a 1544 m.s.n.m) - E2= Grande (762221 UTM East, 8238815 UTM North a 1386 m.s.n.m - E3= Majes1 (769459 UTM East, 8220997 UTM North a 619 m.s.n.m - E4= Majes2 (772525 UTM East, 8198706 UTM North a 373 m.s.n.m) - E5= Camaná (740355 UTM East, 8162246 UTM North a 17 m.s.n.m) - 25% Matherial & Methods Fixtures=2000 lux pH=7.5 + 0.01
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A quick and simple bioassay ecotoxicological allows a quick estimate, evaluating the state of the aquatic ecosystems of the Majes-Camana-Colca watershed region Arequipa, Peruvian in the sampling stations (Taparza, Grande, Majes1, Majes2, and Camana) by inhibition of growth of microalgae Pseudokirchneriella subcapitata 24, 48, 72 hours to Effective Concentration (EC50) at 72 hours compared to Peruvian National Environmental Quality Standards (ECA) guidelines and water quality of the World Health Organization (WHO). Noting that in the sampling stations and Majes2 Majes1 greater inhibition of growth of P. subcapitata were recorded values surpassing thermotolerant coliforms, Aluminium, Manganese, Iron and Total Suspended Solids (TSS) compared with WHO and ECA record high correlated with the percentage of inhibition of growth of P. subcapitata (r = 0.92; p 100%) categorizing as nontoxic; Grande (EC50 = 84.2%) categorizing it as slightly toxic; Majes1 (EC50 = 60.7%) and Majes2 (EC50 = 57.43%) categorizing them as moderately toxic; and Camaná (EC50 = >100%) categorizing as nontoxic.
Transcript of Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirchneriella subcapitata
7/16/2019 Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirchneriella subcapitata
http://slidepdf.com/reader/full/ecotoxicological-bioassays-for-aquatic-ecosystems-with-microalgae-pseudokirchneriella 1/1
Ecotoxicological bioassays for aquatic ecosystems with microalgae Pseudokirch ner iella sub capi tata *Ronald Huarachi Olivera1; Ursulo Yapo2; Alex Dueñas2; Gustavo Mendoza2; Werner Silva2; Giovani Alatrista2; & Rosaura Gonzalez2
1Magister Program in Biotechnology, University of Antofagasta, Antofagasta, Chile. E-mail: [email protected] Web: http://www.researchgate.net/profile/Ronald_Olivera
2Aquatic Biology Laboratory, Academic Department of Biology, National University of San Agustin, Arequipa, Peru, Av. Alcides Carrión s/n
Introduction & Study Objectives
Results and Discussions
Conclusions
Microalgae Pseudokirchneriel la subcapitata
Pseudokirchneriella subcapitata
Olav Skulberg (1959)
river Nidelva, Akershus, Noruega
Bioassayecotoxicological Isolated
Growth
inhibition
Effectiva
ConcentrationEC50
E5=Camaná
Sampling
points
Effective Concentration (EC%) to 72 hours
EC5 EC10 EC15 EC20 EC25 EC50 TU50 C.T.
E1 23.9
7
36.31 48.05 60.04 72.68 157.14 0.64 5
E2 2.59 5.59 9.39 14.18 20.2 84.2 1.19 4E3 27.3 32.54 36.66 40.3 43.72 60.7 1.65 3
E4 14.6 19.72 24.2 28.47 32.72 57.43 1.74 3
E5 89.1 93.88 96.99 99.39 >100 109.57 0.91 5
C.T.:Categories toxicological; 5:Non toxic; 4:slightly toxic;3:Moderately toxic.
Growth Inhibition of Pseudokirch neriel la subc apitata as areas of the watershed Camana - Majes
72 hours
Watershed Camana Majes
Southern Peruvian
Arequipa Cusco Puno
Activities
Economic
Agricultural-livestock
Mining
Fishery
The aim of this study was to estimate ecotoxicological
bioassays quick and simple in river systems as
Taparza, Grande and Camana rivers - Majes (Arequipa,
Peruvian) by the median Effective Concentration (EC50)
categorises as the range of toxicity and inhibition
growth Pseudokirchneriel la subcapitata compared to
physicochemical analysis that exceed the values of the
Peruvian National Environmental Quality Standards
(EQS) and guidelines for water quality of the World
Health Organization (WHO)
Parameter Analysis Unit E1 E2 E3 E4 E5
EQS Category 3 EQS Category 4 WHO
Irrigating with long stems and low animals drink Conservation aquatic
environment
Coliform Laboratory NMP/100mL - 490 79 7900 49 1000 1000 2000 n.a.
Al Laboratory mg/L 0.55 0.02 18.05 19.64 1.68 5 5 - 0.2
Mn Laboratory mg/L 0.0202 0.042 0.743a 0.814a 0.153a 0.2 0.2 - 0.1
Fe Laboratory mg/L 0.382 0.026 15.12a 19.51ª 1.893a 1 1 - n.a
STS Laboratory mg/L 24.94 <3 1315a 149ª 114a - - ≤25-100 n.a
Parameters that exceed the EQS and WHO
E3= Majes1
E4=Majes2
E2=GrandeE1=Taparza
24 hours 48 hours
Ecotoxicological bioassays with the microalga Pseudokirchneriel la subcapitata in watershed Camana, Majes from region Arequipa,
Peruvian showed higher inhibition of growth and low growth rate in the E3 (Majes1) and E4 (Majes2) stations with a median Effective Concentration (EC50): E3
(EC50 = 60.7%) and E4 (EC50 = 57.43 %) categorizing them as moderately toxic. This inhibition may be associated with concentrations thermotolerant coliform, Al, Mn ,Feand total dissolved solids in the E3 and E4 stations that exceed national standards Peruvian water quality(EQS) and the guidelines World Health Organization (WHO) .
Growth rate of
P. sub capitata as
areas from the
waterwhed
Camana - Majesupto 72 hours a
bioassay.
Graphs showing the concentration-response effects bioassays sampling
points wi th growth Pseudokirchneriel la s ubcapitata with Effective
Concentration (EC) at different percentages to 72 hours.
Our study has two important results. First, the intensity of the most polluted
spots in the river systems by inhibiting growth of P. subcapitata compared toPeruvian Environmental Quality Standards (EQS) and water quality
guidelines of the World Health Organization (WHO). Second, growth of
P. subcapitata the median effective concentration (EC50) and Toxicity Units
(TU) at 72 hours bioassays sampling points compared to toxicologicalcategories established by Bulich (1982) and Coleman & Quereshi (1985) .
Counting in Neubauer chamber Microscopic view of microalgae P. subcap itata (100X)
- Bulich A. A practical and reliable method for monitoring the toxicity of aquatic samples.
Process Biochem. 1982; 17: 45-47..
- Coleman RN, Qureshi AA. Microtox and Spirillum volutans test for assessing toxicity
of environmental samples. Bull. Environ. Contam. Toxicol. 1985; 35: 443-451B
Bibliography
STUDY AREA
Pseudokirchneriel la subcapitata strain was obtained from CENMA
(Centro Nacional del Medio Ambiente) Universidad de Chile,Región Metropolitana, Santiago, Chile.
Cultivated in 250 mL Erlenmeyer flasks with 100 mL culture medium CULTURE CONDITIONS
Tº=24 - 25ºC
BIOASSAY ECOTOXICOLOGICAL WITH P. subcap itata
- Negative Control (Solution Buffer, 15 mg NaHCO3)
- 6,25%
- 12,5%
- 50%
- Positive Control (Water problem sample)
EFFECTIVE
CONCENTRATION (EC50)
-Toxicity Units = TU
TU= (EC50)-1 x 100
µ = lnN – lnNo
ΔtWhere:
N = cell density at the end of trial
No = nominal initial cell densityΔT = time interval considered
Toxicological
categories aqueous samples according to the results obtained using the
ecotoxicological bioas
says Pseudokirchneriel la subcapitata (Bulich 1982; Coleman &Quershi 1985)EC50 Toxicity Category Rank Toxicity Units(TU)
< 25 Very Toxic 1 >4
25 - 50 Toxic 2 2 - 3.99
51 - 75 Moderately toxic 3 1.33 - 1.99
76 - 100 Slightly toxic 4 1.01-1.32
>100 NonToxic 5 < 1
- E1= Taparza (791888 UTM East, 8333378 UTM North a 1544 m.s.n.m)
- E2= Grande (762221 UTM East, 8238815 UTM North a 1386 m.s.n.m
- E3= Majes1 (769459 UTM East, 8220997 UTM North a 619 m.s.n.m
- E4= Majes2 (772525 UTM East, 8198706 UTM North a 373 m.s.n.m)
- E5= Camaná (740355 UTM East, 8162246 UTM North a 17 m.s.n.m)
- 25%
Matherial & Methods
Fixtures=2000 lux pH=7.5 + 0.01
