Echo® Acoustic Liquid Handling for Regulated Laboratories · 2019. 5. 8. · Echo® Liquid...
Transcript of Echo® Acoustic Liquid Handling for Regulated Laboratories · 2019. 5. 8. · Echo® Liquid...
Echo®
for Regulated Laboratories
BROCHUREVersion 2.0 | MARCH 2019
© 2019 LABCYTE INC. All rights reserved.
LABCYTE INC.170 Rose Orchard WaySan Jose, CA 95134 USA
Toll-free: +1 877 742−6548 | Fax: +1 408 747−2010
Echo® Acoustic Liquid Handling for Regulated LaboratoriesREDEFINE SCIENTIFIC INNOVATION WITH ACOUSTIC LIQUID HANDLING
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Echo® Liquid Handlers for Regulated Laboratories
Re-imagine Your Science and Research Bioassay development and deployment are challenging. Complex workflows and stringent success criteria require liquid handling solutions that offer
both the flexibility to meet unique workflow needs and the exceptional reproducibility the work demands.
Using an innovative, non-contact dispensing technology, Labcyte Echo® Liquid Handlers deliver the utmost flexibility with any-well to any-well transfers
and unparalleled accuracy and precision, even when working with nanoliter transfer volumes. Pooling large numbers of assay components and sample
randomization, commonly challenging for standard automated liquid handlers, are straightforward with Echo® Liquid Handlers.
Echo® Liquid Handlers are transforming how researchers approach their science delivering significant cost reductions both through assay
miniaturization and elimination of pipette tips.
BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES
www.labcyte.com/echo-regulated
Inverted destination plate
Transducer(acoustic energy generator)
Source plate2.5 nL or 25 nLdroplet transfers
Fluid TYPE A
Fluid TYPE B
Fluid TYPE C
Fluid TYPE D
FIGURE 1: Acoustic droplet ejection - The transducer is positioned below the source microplate well and emits focused sound energy repeatably to the meniscus of the fluid to be transferred. A stream of 2.5 nL or 25 nL droplets (model dependent) is reliably ejected into a well of an inverted destination microplate.
Using Dynamic Fluid Analysis™,
Echo® Liquid Handlers use sound
energy to determine fluid composition,
fluid height, and the power needed to
eject a precise volume of fluid into a
destination well. Dynamic Fluid Analysis
adjusts transfer parameters in real-time
to compensate for changes in fluid
height (well volume) and fluid properties
without recalibration.
On-the-Fly Adjustments to changing fluid properties
• Real-time adjustment to changing fluid types, volumes, or properties without user intervention
• Repeatable transfer of complex reagent sets or inconsistent reagents
DYNAMIC FLUID ANALYSIS™
Innovative Technology and Unparalleled Performance Echo® Liquid Handlers rely on patented technologies and novel methodologies to change how liquid handling is used in applications throughout life
science. With Dynamic Fluid Analysis™ Technology and Acoustic Droplet Ejection, the Echo® Liquid Handler enables researchers to transfer a broad
spectrum of fluid types with a degree of flexibility and reliability not possible with traditional liquid handling technologies.
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LABCYTE INC.
www.labcyte.com/direct-dilution
With the ability to transfer low nanoliter volumes of liquid, Echo® Liquid Handlers can produce dose-response and standard curves free of the errors
typically found using traditional tip-based liquid handlers and serial dilution. Echo® Liquid Handlers create a series of dilutions by directly transferring
decreasing volumes of stock sample to individual assay wells. Since each concentration point along the dilution curve is created individually, instead of
serially, the risk of propagating errors along the curve is eliminated — a significant improvement to data quality.
Simultaneous movement of the acoustic transducer and destination
plate during transfer enables Echo® Liquid Handlers to transfer from
any source well to any destination well faster than traditional liquid
handling technologies that require time to transport samples from
source wells to destination wells and to change tips between transfers.
for a more reliable dilution of samples and reagents
for superior speed and flexibility
Direct Dilution
Any-Well to Any-Well Transfer
FIGURE 3: Examples of rapid cherry picking, sample pooling, and combination screening from any source well to any destination well, at any volume.
A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
BCDEFGHIJKLMNOP
A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
BCDEFGHIJKLMNOP
Destination Plate
Source Plate
A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
BCDEFGHIJKLMNOP
A1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
BCDEFGHIJKLMNOP
Source Plate
Destination Plate
Potency Assays with Direct Dilution
Dir
ect
Dilu
tio
n
Direct Dilution
% In
hib
itio
n
Inhibitor Concentration (M)
100
80
60
40
20
0
7.5 nL25 nL
250 nL
2.5 nL
10 mM Stock
2.5 nL
30 nL92.5 nL100 µM Intermediate
10 nL 2.5 nL
37.5 nL115 nL1 µM Intermediate
12.5 nL 5 nL
1E-10 1E-09 1E-08 1E-07 1E-06 1E-05
FIGURE 2: Example of an 11-point curve created using direct dilution. The image on the left shows how the desired concentrations are created by transfers directly from the stock sample and two intermediate dilutions of the stock sample. The resulting IC50 curve is shown on the right.
Direct Dilution
Potency Assays with Direct Dilution
Dir
ect
Dilu
tion
Potency Assays with Direct Dilution
Dir
ect
Dilu
tio
n
Direct Dilution
% In
hib
itio
n
Inhibitor Concentration (M)
100
80
60
40
20
0
7.5 nL25 nL
250 nL
2.5 nL
10 mM Stock
2.5 nL
30 nL92.5 nL100 µM Intermediate
10 nL 2.5 nL
37.5 nL115 nL1 µM Intermediate
12.5 nL 5 nL
1E-10 1E-09 1E-08 1E-07 1E-06 1E-05
Inhibitor Concentration (M)
% In
hib
itio
n
⊲ Pool and normalize samples, reagents, and primers by transferring different volumes from a collection of source plate wells
⊲ Cherry pick screen 'hits' in minutes for secondary screening
⊲ Quickly create combinations of samples and reagents in varying concentrations
⊲ Define transfer regions to create custom layouts
⊲ Compress libraries into interleaved patterns or separate quadrants
⊲ Offset transfers to any position in a well
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BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES
Echo® Liquid Handlers Models
Cost-effective Plate Preparation and Superior Data Quality
Sample Management
Biologically Relevant Assays with Unmatched Data Quality
Cell-based Assays
Simplify Assay Workflows with Precise Reagent Transfers
Biochemical Assays
Enable Cost-Effective, Earlier Safety Screening
ADME-Tox
Maintain 21CFR11 Compliance While Using Echo® Liquid Handlers
Bioassays
Significantly Improving Workflows
Enabling Miniaturization with Unparalleled Throughput and AccuracyReagents, compounds, and samples used throughout the drug discovery process are transferred efficiently and accurately with Echo® Liquid Handlers.
With various throughput options and fluid transfer capabilities, you can use the Echo system from the early stages of drug discovery to drug
development and deployment.
Echo® 525RHigh Volume Transfer
Acoustic Liquid Handler
Echo® 550R SeriesFirst Generation
Acoustic Liquid Handler
Echo® 650R SeriesNext-Generation
Acoustic Liquid Handler
Transfer Volume: 25 nL – 5 μL Transfer Volume: 2.5 nL – 2 μL Transfer Volume: 2.5 nL – 2 μL
www.labcyte.com/applications
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0.5 µLCells
10 µLCellTiter-Glo reagent
QS to 9 µLDirect Dilution
of TNF or Antibodyfor Dose Response on
media prefill
0.5 µLEC80 TNF challenge
or media
30-minuteIncubation
24-hourIncubation
Luminescent Readout
PHERAstar
384-well Low Volume Plate
LABCYTE INC.
Miniaturizing Bioassays with Acoustic Liquid Handling
Reduce Assay Volumes, Not Performance Successful miniaturization of bioassays requires the highly reproducible and accurate transfer of nanoliter and microliter volumes of cells, reagents,
and samples. Echo® Liquid Handlers deliver small volumes of these components accurately and precisely with flexibility and speed, enabling assay
miniaturization at previously unattainable levels without sacrificing quality. The 25 nL and 2.5 nL resolutions of Echo® Liquid Handlers allow for
direct dilution, whereby standard curves can be generated with an extended detection range and eliminating error-prone serial dilutions. In addition,
acoustic fluid transfers guard against cross-contamination due to the lack of any physical interaction with the liquid.
Host Cell Residual DNA Testing in Reduced Volume qPCR Reactions Traditional methods of quantitating residual host cell DNA are limited by laborious sample preparation protocols and lack of sensitivity. In addition,
recommended total reaction volumes are high to guard against the inaccuracy of human pipetting of sub-microliter reagent quantities. Assay total
reaction volumes can be reduced up to six-fold using Echo® Liquid Handlers, enabling for a large reduction in cost per reaction, additional replicates
and more confidence in the results generated.
The Echo® Liquid Handler was used
to reduce volumes of resDNASEQ™
Quantitative DNA Kits using human,
E. coli, and Chinese hamster ovary
(CHO) cell lines. In all cases, the
manufacturer recommended lower
limit of 0.03 pg in a 30 μL reaction
was capable of being lowered to
0.003 pg in a 5 μL reaction.
Representative qPCR traces of the
triplicate human, E. coli, and CHO
resDNASEQ results across multiple
orders of magnitude showing
reproducibility. Quantity of DNA per
reaction is annotated in picograms.
Human, E. coli, and CHO resDNASEQ
qPCR standard curves.
Miniaturized Humira TNF-Alpha L929 Cytotoxicity Bioassay For the Humira TNF-Alpha L929
Cytotoxicity Bioassay, the Echo® 525 Liquid
Handler enables a robust, miniaturized,
automated cell-based 384-well bioassay
workflow. The result is a 10 µL cell-based
assay performed with a single instrument,
which provides additional controls and
replicates in a miniaturized reaction to
reduce sample requirement, cost, and
potential human error.
Echo 655 Transferred resDNASEQ qPCR Kit40
30
20
Mea
n C q
pg DNA
10
0.001 0.01 0.1 1 10 100 1000 100000
Echo 655 Liquid Handler E. coli resDNASEQ34.175
31.175
28.175
25.175
22.175
19.175
16.175
13.175
10.175
7.175
4.175
1.175
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Fluo
resc
ence
(465
–510
)
1.240
Echo 655 Liquid Handler Human resDNASEQ
36.240
32.740
29.240
25.740
22.240
18.740
15.240
11.740
8.240
4.740
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Fluo
resc
ence
(465
–510
)
Echo 655 Liquid Handler CHO resDNASEQ
47.655
43.155
38.655
34.155
29.655
25.155
20.655
16.155
11.655
7.155
2.655
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Fluo
resc
ence
(465
–510
)
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Echo® 21CFR11 Compliance Manager
BROCHURE | ECHO® LIQUID HANDLERS FOR REGULATED LABORATORIES
Echo® Software Applications
Quickly and Easily Develop Protocols for Echo® Liquid Handlers
Labcyte offers a full suite of Echo® Software Applications to enable researchers to quickly and easily create liquid handling protocols for specific
applications with minimal training. Each Echo application is designed around a specific liquid handling workflow and uses a combination of wizards
and graphical interfaces to simplify the creation of plate formats, liquid transfer routines, and output files. Researchers can quickly create a variety
of protocols off-line for Echo® Liquid Handlers and use built-in simulators to validate every transfer before running live. The suite of Echo software
applications enables Echo® Liquid Handlers to quickly and efficiently accomplish any liquid handling task.
Echo®
Array Maker
Echo®
Cherry Pick
Echo®
Combination Screen
Echo®
Dose- Response
Echo®
Plate Audit
Echo®
Plate Reformat
Echo®
Compliance Manager
www.labcyte.com/software
www.labcyte.com/ecm
Roles Guest User Power User Approver Admin
View APPROVED protocols and run reports
Run APPROVED protocols
Create, edit, and run DRAFT protocols
Edit APPROVED protocols
Override approval requests
Archive or delete project data
Disable OBSOLETE protocols from running
Manage and Track Users and Electronic Records
Echo® 21CFR11 Compliance Manager (ECM) locks down and tracks all Echo® Liquid Handler usage, protocol changes, and Echo file outputs using
state-of-the-art security algorithms. Using advanced checksum verification, the software detects any data tampering, assuring data integrity and
peace-of-mind. Compatibility with database management tools minimizes Information Technology infrastructure needs.
ECM offers five user access profiles to actively control and track:
• User access utilizing Microsoft Windows® Active Directory and AD-LDS
• Data organization including SQL database creation, backup, and restoration
• Protocol creation, changes, and approvals
• Echo run log file access and output
Seamlessly integrating with your favorite Echo® Liquid Handler applications, assay development and production
teams enjoy the full functionality of the Echo® Liquid Handler with the appropriate oversight necessary to meet
regulatory tracking and auditing requirements.
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Echo® Qualified Consumables
Service and Support
Maximize Your Instrument Performance
Echo® Qualified Consumables must meet the highest specifications to achieve the exceptional performance expected from Echo® Liquid Handlers.
Only consumables that are made of an acoustically compatible material and are exceptionally flat with extremely low inter- and intra-plate CVs are
considered qualified for use on Echo systems. Factory fluid calibrations developed specifically for Echo® Qualified Consumables support transfers
of a broad range of fluid types, providing maximum flexibility at the highest level of accuracy and precision.
Timely service and preventative maintenance are essential for optimal instrument performance and data quality. Labcyte offers a range of service
contract options to fit every lab’s needs and budget. Labcyte provides global field service support with local personnel in the United States, Europe,
and Asia. In addition to field support, we have support and instrument maintenance facilities in the United States, Europe, and Asia.
Labcyte offers superior application support dedicated to helping you achieve optimal results
from your Echo® Liquid Handlers and Access™ Automation Platforms. As a global organization
that serves customers in the United States, Europe, and Asia, Labcyte is a collaborative partner
that is committed to your success.
LABCYTE INC.
Precision Manufacturing and Exceptional Performance
⊲ 384-Well Polypropylene Microplates
⊲ 384-Well Low Dead Volume (LDV) Microplates
⊲ 1536-Well LDV Microplates
⊲ 384-Well Polypropylene Plus Microplates for aqueous transfer
⊲ 384-Well LDV Plus Microplates for aqueous transfer
⊲ 384-Well Tissue Culture Treated Polypropylene Microplates
⊲ 1536-Well Tissue Culture Treated LDV Microplates
⊲ 96-Tube acoustic sample tube racks (manufactured and sold by Brooks Life Sciences)
⊲ 6-Well Reservoirs
⊲ MicroClime® Environmental Lids
Global Applications Support Team
www.labcyte.com/consumables
www.labcyte.com/service
© 2019 LABCYTE INC. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, Access™, andDynamic Fluid Analysis™ are registered trademarks or trademarks of Labcyte Inc., in the U.S. and/or other countries.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
LABCYTE INC.170 Rose Orchard WaySan Jose, CA 95134USA
Toll-free: +1 877 742−6548 | Fax: +1 408 747−2010
SALESNorth America | +1 408 747−2000 | [email protected] | +353 1 6791464 | [email protected] | +81 03 5530 8964 | [email protected] | +61 39018 5780 | [email protected] | +1 408 747−2000 | [email protected]
All product names and brands are properties of their respective owners.
BRH-ECRE-2.0MAR 2019