easySpiral® Dilute The world's fi rst automatic diluter & plater

A revolution in serial dilutions!

1977Spiral® DSFirst Spiral® plater

1996Spiral® DS+ Automatic Spiral® plater

2010easySpiral®

World's fastest Spiral® plater

PATENTED

2012easySpiral® DiluteWorld's fi rst automatic diluter & plater

PATENTED

Our quality for your lab• Designer and manufacturer for microbiology

• R&D leadership for innovative and reliable products

• Worldwide distribution network in more than 80 countries

• Made in France

Spiral® method: 35 years experienceThe Spiral® method was designed in 1973 to automate the routine work of bacterial enumeration by Dr. Ed Campbell, researcher at the FDA (Food & Drug Administration). With François Jalenques, friend and founder of interscience, they patented an updated method in 1992.

Since then Spiral® automatic platers have been a reference for applications in food microbiology, medical bacteriology, research on food preservatives or cosmetological factors in compliance with the AFNOR V08-100 and ISO 7218 standards.

Today interscience is proud to launch the 4th generation of Spiral® platers with easySpiral® Dilute: the world's fi rst automatic diluter & plater.

F. Jalenques & Dr. Ed Campbell

interscienceR&D center and manufacturing plantMourjou FRANCE

3

interscience is proud to introduce easySpiral® Dilute, a 2-in-1 automatic diluter and plater, which allows you to do 5 x 1/10th serial dilutions. It will then automatically plate on 1 single Petri dish, with a countable range of 30 to 10 million countable CFU/mL.

Place your sample in the beaker

1. The easySpiral® Dilute will automatically dilute the sample

2. The easySpiral® Dilute will automatically plate the sample on a Petri dish

Why use an automatic diluter & plater ?easySpiral® Dilute allows you to save 50 % on consumables and 50% on your time.

Comparison between manual and automatic method below.

Example of 50 samples: Dilution: 10-7 Plating: 10-5, 10-6 and 10-7

Manual method

CONSUMABLES

Dilution

• 400 tubes

• 350 pipet tips

Plating

• 450 Petri dishes

10h10TIME

CONSUMABLES

Dilution

• 350 beakers

Plating

• 150 Petri dishes

5h50

Automatic method

TIME

1

2 Place sample

Dilute

Plate

4

Autoclavable diluent and disinfectant bottles No more 9 mL tube preparation

Automatic disinfectionUsing Overfl ow technology

No cross contamination

PATENTED

One button touchFor 10-5 dilution and plating

PATENTED

High precision glass syringe Typical accuracy of 0.5 %

No more pipet calibration

Direct accessPre-set dilutions and plating

Fast rotating arm5 dilutions and 1 plating in 100 sec. PATENTED

Compact38 cm wide, can be used inside and outside a laminary fl owStainless steel housing

Key features

5

How does it work?

How to count?

Scan® 300Scan® 500Scan® 1200

Automatic colony countingManual colony counting

Dilution

Plating

or

Scan® 100

with Spiral®

counting grid

10-210-1sample

0.5 mL of 10-1 dilution is deposited

Dilution cycle can be repeated as many times as you wish.

10-1sample

4.5 mL of diluent is added to the sample and mixed

10-1sample

0.5 mL of 10-1 diluted sample intake

The above dilution cycle is repeated for the next serial dilution.

sample

Sample intake1

2

1

2

3

sample 10-1

0.5 mL of sample is deposited

Constant modereplicates the manual plating

with a homogenous deposition.50 µL, 100 µL, 200 µL

and USB programmable

allows a decreasing concentration deposition.

50 µL, 100 µL, 200 µL and USB programmable

allows the platingof 3 dilutions on 1 Petri dish.

10 µL inner circle, 27 µL middle circleand 56 µL outer circle

Spiral® mode Circle mode

Before incubation

Examples:

Afterincubation

10-310-1 10-2Homogenous deposition Concentrated Less concentrated

6

The accuracy of easySpiral® Dilute plater is high: 0.5%. How can I check it?

It is easy and automatic.

Press on the test procedure button which fi lls a beaker with 0.5 mL and another one with 4.5 mL. Weight the dispensed volume to ve-rify the results.

Questions

Can I use peptone wateras diluent?

No, because all diluents with nutriments can result in contamination of the machine and of the sample.

You can use any saline solution, for example ringer solution or NaCl solution. As the bacteria stay in the beakers for only a few seconds before going on a nutriment solution on the agar, the dilution with only a saline solu-tion has no consequence on the overall count.

I usually use 9 mL tubes and 1 mL sample...

The easySpiral® Dilute has a 0.5 % precision and typical accuracy on 4.5 mL and 0.5 mL volumes thanks to its high precision HamiltonTM glass syringe.

Common precision for the fi lling of the 9 mL tubes is 2% and pipettes precision for the 1 mL intake is 2%, and they must be often calibrated and checked.

Conclusion, you can obtain much higher precision with volumes cut by half!

What is special aboutthe easySpiral® Dilute ?

easySpiral® Dilute is the only 2-in-1 equipment on the market making automatic serial dilu-tions and standardized plating.

I need to make 10-7 dilutions. How can I do it ?

The easySpiral® Dilute can do as many dilution as you wish. Do a 10-5 dilution, take the last beaker and place it as the mother sample and then proceed with a new automatic dilution sequence.

7

Beakers DB 50sterile beakers

Ref 415 100

Barcode readerbarcode reader

Ref 522 000

Housing protectionfor working outside laminar fl ow

Ref 413 001

Accessories & technical specifi cations

Bottle set connection GL 45for GL 45 bottles

Ref 413 003

easySpiral® DiluteReference 414 000Petri dish diameter 55 mm, 90 mm and 150 mmSyringe capacity 1 000 µLDispensed volume from 10 to 1 000 µLPreset volume dispensed (programmable) 50, 100 and 200 µLDetection in CFU/mL 30 to 10 million CFU/mLPlating modes exponential, uniform and circlesNumber of dilutions at 1/10th for 1 cycle 5Full dilution cycle 100 sec.Full plating cycle 25 sec.Full cycle (disinfection, 5 dilutions, plating of 6 dishes) 3 min. 40 sec.Diluent autonomy 55 cycles of 5 dilutions (with 2L bottles)

Patented circle mode 3 dilutions on one 90 mm Petri dish 6 dilutions on one 150 mm Petri dish

Stylus disinfection system inside and outside by Overfl owOverfl ow technology (patented) 8 bars pressureMonthly cleaning programCleaning capacity 600 cycles (with 2L bottles)Successive plating capacity with the same sample 10 Petri dishes (50 µL)Traceability Excel™ exportProgrammable volume via USBFilling time programmable via USB (for viscous samples) Sample mixing before dilution/plating Controlled by microprocessorDimensions 39 x 42 x 29 cmWeight 15.6 kgPower supply 100-240 V~ / 50 Hz to 60 Hz1 year guarantee (after registration)All stainless steelMade in France

2002/96/ECWEEE

2002/95/ECRoHS ISO

90012008 MADE IN

FRANCE

easySpiral® Dilute is delivered with 1 000 sterile beakers, 1 blue dye for tests, 1 detergent liquid, 3 sets of GL 45 bottle connectors, 55, 90 mm & 150 mm Petri dish ring, Spiral® counting grids: 90 mm & 150 mm, test container stand, power cord, user’s manual, , monitoring CD-ROM software, USB cable.

EN

easy

Sp

iral D

ilute

_B_0

612.

Pic

ture

s an

d in

form

atio

n in

this

bro

chur

e ar

e no

t con

trac

tual

ly b

indi

ng. e

asyS

pira

l® is

regi

ster

ed tr

adem

ark.

INTE

RS

CIE

NC

E r

eser

ves

the

right

to c

hang

e or

imp

rove

the

spec

ifi ca

tions

of i

ts p

rod

ucts

with

out n

otic

e. P

leas

e vi

sit w

ww

.inte

rsci

ence

.fr fo

r cu

rren

t up

dat

es a

nd a

dd

ition

al in

form

atio

n.

Distributor

30, ch. Bois Arpents - 78860 St Nom - FRANCET: +33 (0)1 34 62 62 61 - F: +33 (0)1 34 62 43 03in fo @ in te r sc ience.f r - w w w. in te r sc ience.f r

INTERNATIONAL

CHINA

上海市漕溪路 2 2 2号航天大厦北楼 1 0 0 7室电话 : 021-52541629 - 手机 : 13040616422sales.china@interscience.fr - www.interscience.fr/china

USA & CANADA

199 Weymouth ST. - ROCKLAND - MA 02370 - USAT: + 1 781 792 2133 - F: +1 781 792 2134info@intersciencelab.com - www.intersciencelab.com

www.interscience.fr