(RESEARCH & TRAINING)

An IS/ISO 9001:2015 Organization

3377,, BBEELLGGAACCHHIIAA RROOAADD,, KKOOLLKKAATTAA--770000003377

2018 - 2019

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STATES UNDER JURISDICTION OF REGIONAL DISEASE DIAGNOSTIC LABORATORY (ER)

With the establishment of Regional Disease Diagnostic Laboratory (ER), Kolkata diagnostic facilities have been extended to eastern & north-eastern states viz. Bihar, Orissa, Jharkhand, Sikkim and Andaman & Nicobar Islands.

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PREFACE

Since its inception, Regional Disease Diagnostic Laboratory (ER) has been efficiently

performing its role by concentrating on the specified mandate in areas of disease

surveillance, diagnosis, documentation, imparting training, maintenance of data bank on the

epidemiology of diseases including nationally and internationally important diseases of

livestock and poultry with the ultimate target of up-gradation of livestock health to reduce

loss due to diseases.

All efforts were delivered for application of the latest diagnostic techniques for

diagnosis of different livestock and avian diseases. The Avian Influenza Laboratory has been

functioning effectively by screening of routine sera samples by competitive ELISA for

detection of Influenza Type „A‟ viral antibody and Haemagglutination Inhibition test for

detection of Influenza virus Subtype specific antibody. Routine screening of swab / tissue

samples for Avian Influenza Virus in Embryonated Chicken Eggs (ECE) is being carried out

purposefully. The competence of the laboratory for handling any unusual mortality sample

suspected for HPAI (Highly Pathogenic Avian Influenza virus) has been proved at several

instances. Recently, standardization of PCR for detection of Bacterial pathogens like Listeria

sp., Anthrax bacilli from blood and dried meat & HS specific Bovine Pasteurella sp. from

blood, Mycobacterium tuberculosis from tissue specimen, Mycoplasma sp. from nasal

secretion, milk, synovial fluid, and Babesia sp., Theileria sp., Anaplasma sp. from whole

blood had been done successfully. PCR has been standardised satisfactorily for diagnosis

of viral diseases like PPR, Lumpy Skin Disease, Goat Pox, Classical Swine Fever, Duck

Viral Enteritis and New Castle Disease also. qPCR technique is being routinely employed for

screening of Bovine Frozen Semen for BoHV-I viral DNA. Standardization of the pesticides

like Cypermethrin, Deltamethrin, Endosulfon, Quinalphos, BHC, Malathion, Monocrotophos,

Phosphomidon, Mycotoxin like Aflatoxin-B1, B2, M1, G2 and Ochratoxin has been done in

HPLC Laboratory. Two antibiotics Oxytetracycline and Ceftriaxone have also been

standardized. Analysis of Pesticide and Mycotoxin in specific samples has been done

successfully. BSE surveillance work and histopathological examination for diagnosis of

several other diseases are being performed regularly. Regular monitoring of the

microbiological parameters of Frozen Semen Bull stations, Bull Mother Farms of different

states is executed as per MSP laid down by the DADF, Government of India.

Standardisation of PCR technique for BoHV-I has been achieved and multiplex-PCR for

different species of Brucella has been accomplished previously.

Further, RDDL (ER), Kolkata is also imparting training to the officials attached to

disease diagnostic work of the dependant states and U/T especially on screening of serum

samples for sero-surveillance work against Avian Influenza, „PCR Technique‟ and upgrading

the knowledge against new techniques of different disease diagnosis including „Sexually

Transmitted Diseases in Bovine‟.

Having gathered the experience of this and previous years, we along with the co-

operation of all will strive to continue performing all the designated works with excellence in

the future too.

(DR. BASUDEB MAITY)

Joint Director, RDDL (ER) Institute of Animal Health & Vety. Biologicals (R&T)

Belgachia, Kolkata

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CENTRAL SECTOR SCHEME OF

DEPARTMENT OF ANIMAL HUSBANDRY & DAIRYING

REGIONAL DISEASE DIAGNOSTIC LABORATORY

(EASTERN REGION)

ANNUAL REPORT

2018-2019

1. Project Title Central Sector Scheme Animal Disease Management and Regulatory Medicine, Department of Animal

Husbandry, Dairying and Fisheries, Ministry of Agriculture, Government of India.

2. Sanction No. F. No. 53-83/97-LDT (LH)

3. Date of Commencement July, 1999

4. Report Period 01-04-2018 to 31-03-2019

5. Head of Regional Disease Joint Director Diagnostic Laboratory Regional Disease Diagnostic Laboratory

Eastern Region

6. Location Institute of Animal Health & Veterinary Biologicals (R&T)

37, Belgachia Road, Kolkata- 700037

An IS/ISO 9001:2008 Organization

Ph. : 033-2532-8033/033-2546-7087/033-2556-5021

Telefax : 033-2532-8033

e-mail ID : erddl_kolkata@yahoo.co.in

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REGIONAL DISEASE DIAGNOSTIC LABORATORY EASTERN REGION

M A N D A T E 1. To serve as regional referral laboratory for economically important livestock

diseases (bacterial, viral, parasitic etc.) and study of effects of any drugs/pesticides

residue/toxicants in the livestock products by providing diagnostic services (both

primary and confirmatory) to the states of the region.

2. To maintain a data bank on the epidemiology of the economically important diseases

of the region and circulate to all the states on a periodic basis.

3. Be responsible to collect and transmit all isolates of viral, bacterial and parasitic

agents to the National Repository to be maintained at the National Reference

Laboratory.

4. To follow the approved standard reagents and techniques for generation of the

diagnostic results.

5. To train scientists/officers from State Disease Diagnostic Laboratories of the region

to update different technology for diagnosis of animal diseases and provide

consultancy and expert services to the states of the region.

6. To undertake special programme for disease surveillance/diagnosis as per the

direction of Govt. of India from time to time in view of epidemiological significance

and importance etc.

7. Any data generated by the RDDL could be published only after prior permission

obtained from Department of Animal Husbandry, Dairying and Fisheries, Government

of India.

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STAFF PATTERN OF

REGIONAL DISEASE DIAGNOSTIC LABORATORY

EASTERN REGION, KOLKATA

Sl. No. Name of the post

Sanctioned strength

Present incumbent

1. Joint Director, RDDL (ER) 01 Dr. Basudeb Maity

M.V.Sc (Veterinary Pathology)

2.. Specialist (Diagnostic Pathology) 01 Dr. Debasis Chatterjee

M.V.Sc. (Veterinary Pathology) (Acting)

3. Specialist (Diagnostic Virology) 01 Dr. Shamindra Nath Sarkar

M.V.Sc. ( Veterinary Virology)

4. Specialist ( Sero Epidemiology )

01

Dr. Indrajit Kar

Ph. D. (Veterinary Pathology)

5. Specialist (Epidemiology &

Informatics) 01

Dr. Arnab Das

Ph. D. ( Veterinary Epidemiology & Preventive Medicine)

6. Specialist (Diagnostic

Parasitology)

01 Dr. Rabindra Nath Sikari

M.V.Sc. (Veterinary Parasitology)

7. Specialist (Diagnostic Bacteriology

& Mycology)

01 Dr. Anup Singha

M.V.Sc.( Veterinary Bacteriology)

8. Specialist (Diagnostic

Biochemistry & Toxicology)

01 Dr. Sumit Chowdury

Ph. D. ( Veterinary Biochemistry) (Acting)

9. Laboratory Technician 09 Vacant

10. Laboratory Attendant 11 Vacant

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ANNUAL ACTION PLAN AND TECHNICAL PROGRAMME

FOR RDDL (ER), KOLKATA

2018 - 2019

Technical Programme for the year 2018 -19 as follows: 1.To provide referral and consultancy services to the state diagnostic laboratories for economically important Livestock and poultry diseases. 2. Development of epidemiological Data Bank including epidemiological maps of economically important livestock diseases of the region. 3. Screening of Bulls, teaser animals and bovine semen against sexually transmitted diseases including estimation of microbial load in Frozen Semen Straw in the region. 4. qPCR based diagnosis with special reference to detection BoHV-1 in bovine semen sample. 5. Surveillance work of infectious causes of abortion in Livestock population in organized farm and in the rural areas. 6. Surveillance work on BSE based on Histopathological studies and ELISA based detection of MBM from cattle feed. 7. Screening of brain samples from Bovine Sp. exhibiting neurological disorders for Listeriosis. 8. Immune status studies on Ranikhet Disease in vaccinated organized and backyard poultry population. 9. Application of molecular biological techniques for routine diagnosis of different livestock & poultry diseases (other than Avian Influenza) with special reference to Capri pox, PPR, Classical Swine Fever, Duck Viral Enteritis, Duck Viral Hepatitis, New Castle Disease, Brucellosis, Listeriosis, Anthrax, HS, BQ, CCPP, Tuberculosis, Fowl Adenovirus, Contagious Ecthyma, Marek‟s Disease etc. 10. Routine application of PCR techniques for diagnosis of haemoprotozoan infection in cattles like Babesiosis, Anaplasmosis, Trypanosomiasis and Theileriosis etc.

11. Routine Sero-surveillance work and antigen detection in swab / tissue samples of Avian Influenza as per guideline of Govt. of India and framed surveillance strategies adopted by different states under jurisdiction

of RDDL (ER), Kolkata.

12. Diagnosis of Rabies infection in post-mortem material by immuno-chromatographic technique.

13. Proximate Analysis of animal and poultry feed.

14. A) To impart analytical facilities of pesticides, antibiotics and drug residues in livestock and poultry

products by HPLC. B) Modernization of HPLC by installing Gas Chromatography – Mass Spectroscopy.

15. A) Study of nutritional status including trace elements in infertility cases. B) Clinical Haematology and Bio-chemistry to aid disease diagnosis.

16. Soil testing of Anthrax spore in the endemic area.

17. Testing of export / import materials.

18. Maintenance of Repository of infective agents isolated from the Eastern Region.

19. Organizing training programme for veterinary & para-veterinary work force.

20. Detection of microbial agents responsible for clinical and sub-clinical mastitis and its antibiogram.

21. Monitoring of State Sponsored Scheme on disease investigation.

22. Monitoring of Centrally Sponsored Scheme of NPRE.

23. Surveillance on Tuberculosis and Johne’s disease by Tuberculin / Johnin Sensitivity Test.

24. DNA sequencing of repository of infective agents isolated from the different states under the jurisdiction of

RDDL(ER).

25. Surveillance programme on Glanders as per GOI guideline.

26. Sero-Surveillance of different diseases of livestock diseases with special reference to, CCPP, Blue Tongue,

etc.etc

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ACTIVITY REPORT OF RDDL (ER)

2018-2019

A. Maintenance of Data Bank on epidemiology on various Disease encountered in the Region (as per technical programme-2):

DISEASE INCIDENCE REPORTS OF EASTERN REGION*

* As per the data available with ERDDL, Kolkata

Sl. No.

Disease West

Bengal Bihar Odisha

Andaman

& Nicobar Islands

Sikkim Jharkhand Total

1. Anthrax 5 0 3 0 0 23 31

2. Anaplasmosis 9 0 0 0 33 2519 2551

3. Avian Influenza 0 5 9 0 0 0 14

4. Avian Salmonellosis

0 0 0 254 0 0 254

5. Babesiosis 19 11 22 0 3 4008 4063

6. Black Quarter 6 1 3 0 0 92 99

7. CCPP 0 0 0 0 0 0 0

8. CRD 2 0 0 0 0 0 2

9. Duck Cholera 0 0 0 0 0 0 0

10. Duck Plague 0 0 0 0 0 0 0

11. Foot & Mouth Disease

62 10 52 1 40 3423 3588

12. Fowl Cholera 0 0 0 0 0 0 0

13. Fowl Pox 3 0 26 28 0 0 57

14. Goat Pox 10 1 5 0 0 28 44

15 H.S. 9 1 1 0 1 62 74

16 I.B.D. 5 0 0 4 0 0 9

17 PPR 11 4 6 0 0 3221 3242

18 Rabies 2 3 0 0 0 474 479

19 Ranikhet Disease 34 0 0 15 0 0 49

20 Swine Fever 1 2 0 0 42 86 131

21 Theileriosis 71 2 4 0 6 3013 3013

22 Trypanosomiasis 4 11 0 0 0 7548 7562

23 Coccidiosis 16 0 31 146 686 5823 6702

OUTBREAK OF PPR, GOAT POX IN WEST BENGAL DURING 2018-19

OUTBREAK OF FMD, ANTHRAX, HS, BQ IN WEST BENGAL DURING 2018-19

OUTBREAK OF RD, IBD, FOWL POX IN WEST BENGAL DURING 2018-19

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B. To provide referral and consultancy services to the state diagnostic

laboratories for economically important livestock and poultry diseases

(as per technical programme-1) :

1. Provided diagnostic services to Frozen Semen Bank, Cuttack, Odisha and Bihar

Livestock Development Agency (BLDA), Patna against sexually transmitted diseases.

2. Imparted sero diagnostic support against Brucellosis, IBR, BVD, Campylobacteriosis, and

Trichomoniasis for Karfector Farm, Sikkim.

3. Provided molecular biological diagnosis against common Haemoprotozoan Diseases for

the state of Sikkim, Bihar, Odisha and Jharkhand.

4. Provided diagnostic support in connection with PPR outbreak in Eastern Region.

5. Provided diagnostic support in connection with detection of Pasteurella multocida genome

for the state of Odisha.

6. Provided diagnostic support in connection with Classical Swine Fever outbreak in Eastern

Region.

7. Performed examination of all the serum and swab / tissue samples including Live Bird

Market (LBM) Samples for surveillance of Avian Influenza for different states under

jurisdiction.

8. Organized Residential Training on Common Diagnostic Technique for diseases of

Livestock (30/11/18 & 1/12/18) as per the mandate of RDDL for officers of different states

under jurisdiction of RDDL (ER).

9. Students of final year B.V.Sc & A.H. from WBUAFS, Kolkata attended to different section

of this Institute in batch wise for training on practical aspect of disease diagnosis.

10. Provided diagnostic support (PCR based) against Duck Viral Enteritis for Andaman and Nicobar Islands.

11. Imparted diagnostic support (PCR based) against NDV for state of Bihar.

12. Performed mycotoxin analysis of feed samples and residue analysis of pesticides and

herbicides of tissue samples for state of Bihar and Odisha.

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C. Screening of bulls, teaser animals and bull semen against sexually transmitted

diseases including estimation of microbial load in frozen semen straw (as per technical programme-3, 4) :

As per mandate of ERDDL different samples were received/collected from organized/unorganized farms in West Bengal and other States during this period. Different tests undertaken in the laboratory during the period of April, 2017 to March, 2018 and results are shown in different tables.

C-1. Serological examination reports of different sexually transmitted diseases :

Source

Brucellosis IBR

Remarks Total Samples

P N Total

Samples P N

Organized Farms of

West Bengal 2702 19 2288 1304 597 707

P= Positive N= Negative Unorganized

Farms of West Bengal

811 16 795 133 23 110

OTHER STATES

F S B, Cuttack, Odisha

115 0 115 115 23 92

CCBF, Chiplima,

Odisha 261 0 261 261 12 249

CCBF, Chiplima, Odisha

110 0 110

ADRI, Phulnakhara, Odisha

14 0 14

ADDL, Dollygung

A & N Islands

Bov

429 0 429 429 166 263

Cap 84 0 84

IAH&P, Bihar

State Rural Livelihoods Mission, Bihar

Bihar Livestock Dev.

Agency Patna, Bihar

44 0 44 44 24 20

Karfector Farm Sikkim 54 3 51 15 01 14

TOTAL 4626 186 5486 2301 846 1455

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C-2. Serological examination on Bovine Viral Diarrhoea (BVD) for Antigen Detection :

Name of the Farm/ Place

Total No. Animals Tested

No. of Positive Animals

No. of Negative Animals

Organized Farms of West Bengal

373 0 373

Other States

IAH&P, Bihar

State Rural Livelihoods Mission, Bihar

Bihar Livestock Dev.

Agency Patna, Bihar

22 0 22

TOTAL 395 0 395

C-3. Serological examination on Leptospirosis :

Name of the Farm/ Place

Species

Total No. Animals Tested

No. of Positive Animals

No. of Negative Animals

ADDL, Dollygung

A & N Islands

Cattle 25 1 24

Goat 66 3 63

TOTAL 91 4 87

C-3. Estimation of microbial load in Frozen Semen straw (as per technical programme-3):

Source Total Sample tested Satisfactory

Unsatisfactory

Frozen Semen Bull Stations/ Sperm Stations West Bengal

643 623 20

SDDL of other States Samples not

received - -

TOTAL 643 623 20

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D. Surveillance work on infectious causes of abortion in livestock population

(as per technical programme-5) :

D-1. Cultural examination reports on different sexually transmitted diseases :

Source

Type of

samples

Campylobacteriosis Trichomoniasis

Samples tested

Positive Negative Samples tested

Positive Negative

Frozen Semen Bull Stations /

Sperm station West Bengal

Preputial washing

227 0 227 227 0 227

IAH&P, Bihar

State Rural Livelihoods Mission, Bihar/

Bihar Livestock Dev. Agency

Patna, Bihar

44 0 44 44 0 44

FSB, Cuttack, Odisha 115 0 115 115 0 115

TOTAL 386 0 386 386 0 386

E. q-PCR based detection:

E-1 q-PCR based detection of BoHV - I in neat semen

(as per technical programme Sl. No. 4) :

As per direction laid down in the “OIE Terrestrial manual (2009) for standardization of Diagnostics and vaccines”, the recommended tests for International trade for IBR is Real time (quantitative)-PCR technique. As per proposed MSP of Govt. of India for application of q-PCR, a resolution has been taken in mid-year of 2012-2013, which was incorporated in MSP. We have standardized the test in the year 2014 - 2015. Results of q-PCR based detection of frozen semen straws in batches during the year 2018-2019 are stated below:

Source Total Sample tested

(in batches) Positive

Negative

Frozen Semen Bull Stations/ Sperm Stations West Bengal

10137 1386 8751

Other States

Frozen Semen Bank, Cuttack, Odisha

1125 235 890

BLDA, Patna, Bihar 601 02 599

TOTAL 11863 1623 10240

18 F. Surveillance work on Tuberculosis (as per technical programme Sl. No. 23):

During the year 2018-2019, the Instt. of Animal Health & Vety. Biologicals (R&T), Kolkata, as per RDDL mandate, tested 945 nos. of animals for tuberculosis in bovine population and in wild life using single interdermal test by tuberculin PPD with no reactor. In case of Johne‟s disease there was no reactor animal out of 634 animals tested.

F-1. Tuberculin Sensitivity Test at Govt. Farms/ Private Farms / organization:

Table 1 : Tuberculin Test ( SID )

Sl. No. Name of the Farm/ Institution No. of animal Tested

No. Of Reactor Animal

No. of Doubtful Animal

No. of Non -

Reactor animal

01 Ramkrishna Mission Bidyapith DairyFarm,Purulia 48 0 06 42

02 FSBS, Salboni, Mednipur ( W ) 107 0 0 107

03 BMF, Salboni,Mid.(W) 199 0 0 199 04 Ramkrishna Ashram KVk,Nimpith,24 pgs(S) 81 0 01 80

05 FSBS,Salboni,Mid (W) 14 0 0 14 06 FSBS,Khapuria,Cuttack,Orissa 115 0 0 115

07 BCRU,BBRBMF,Khapuria,Orissa 22 0 0 22

08 BCRU,Chiplima,Sambalpur 89 0 0 89 09 CCBF,Sunabeda,Koraput,Orissa 270 0 0 270

Total 945 0 07 938 F-2. Johnin Sensitivity Test at State Farms/ Private Farms/ organization (as per technical programme Sl. No. 23:

Table 2 : Johnin Test ( SID ) Sl.

No. Name of the Farm/ Institution No. of

animal Tested

No. Of Reactor Animal

No. of Doubtful Animal

No. of Non - Reactor animal

01 Ramkrishna Mission Bidyapith DairyFarm,Purulia

48 0 0 48

02 FSBS, Salboni, Mednipur ( W ) 107 0 0 107

03 BMF, Salboni,Mid.(W) 199 0 0 199

04 FSBS,Salboni,Mid (W) 14 0 0 14

05 FSBS,Khapuria,Cuttack,Orissa 115 0 0 115 06 BCRU,BBRBMF,Khapuria,Orissa 22 0 0 22

07 BCRU,Chiplima,Sambalpur 89 0 0 89 08 CCBF,Sunabeda,Koraput,Orissa 270 0 0 270

634 0 0 634

N.B: Since June-2018,there were no TB/JD,PPD available inTBC Unit, because IVRI,Izzatnagar was unable to supply the same till March ,2019.

19 F-3. Total Test Performed:

G. Surveillance work on BSE by Histopathological studies and ELISA based diagnosis

of brain samples (as per technical programme-6) : Active Surveillance:

A total of one hundred fifty (188) bovine brain samples were received in ice pack from West

Bengal, Bihar, Odisha, Andaman & Nicobar Island and Sikkim. Out of 188 brain samples 58

brain samples(clinical cases) were received from different places of West Bengal, Sikkim,

Andaman & Nicobar, Odisha, Bihar and Jharkhand exhibiting neurological sign before death.

The coronal section from different anatomical sites particularly obex of medulla, cerebellar

peduncle, rostral colliculi and others were cut and fix immediately in 10 % formalin to avoid

autolytic changes. After fixation tissues were processed, cut and stained with Haematoxylin

and Eosin (H&E) and Seller’s stain as per standard procedure for microscopical examination.

None of the brain sample screened had hallmark lesion suggestive to BSE. Though some of the

samples taken from clinical suspect cases having few nervous sign showed mostly vascular or

neurodegenerative changes

But a letter was received from Joint Commissioner (LH), Department of Animal Husbandry,

Dairying & Fisheries, GOI, Krishi Bhaban, New Delhi vide.No.K-11053/28/2016-LH of the Dated

20/03/2019 where it is clearly instructed that OIE has recently recommended India to revise

its testing protocol for BSE to ensure compliance with the Terrestrial Manual when

reconfirming its BSE risk status in November 2019 and considering the fact that the samples

examined by histopathology and resulting negative for BSE should not be reported in BSE

surveillance table. Only samples examined by ELISA as Primary test should be reported in the

BSE surveillance table.

The bovine brain samples were stored in -20°C deep freezer for screening with BSE

ELISA test kit (after procurement of the BSE ELISA kit) as per recent OIE guideline and 186

samples are screened to detect Listeria species and clinical brain sample for Rabies (negri

bodies) by Seller’s staining. The entire Brain sample screened for Listeriosis and Rabies are

also found negative. G-1. Target and achievement of receiving Bovine Brain Samples (clinical suspect cases) from different States of ERDDL : Sl.

No.

Name of the

States

Target

from clinical

suspect case

No of samples received Total no of

brain sample

received

Shortfall of

clinical suspect

cases Slaughter

house

Clinical

suspect case

1. Bihar 17 00 15 15 02

2. Jharkhand 09 - 00 00 09

3 Orissa 16 - 16 16 00

4. Sikkim 05 - 05 05 00

5 West Bengal 20 130 20 150 00

6 A & N Island 02 - 02 02 00

Total 69 130 58 188 11

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Sl. No.

Name of the Test No. of Animal

Tested No. of Reactor

animal No. of Doubtful

animal No. of Non-

reactor animal

1 Tuberculin Sensitivity Test 945 - 7 938

2 Johnin Sensitivity Test 634 - - 634

TOTAL 1579 - - 1572

G-2. General characteristic of animals presented at different slaughter house / Farm / village under ERDDL :

Species Bovine

Breed Indigenous, Gir, Jersey Cross and Murrah & Chilika Buffalo

Age Above 2 yrs to 10 yrs 6 months.

Sex Male & Female

Place of Origin Different parts of West Bengal, Bihar, Andaman& Nicobar

Island, Orissa, Sikkim .

Health Status 80% of animals were apparently healthy and 20% animals health

conditions are very poor.

External Appearance &

Fitness For Work

Mostly old aged, weak, emaciated debilitated and unfit for work

and milking purpose.

Health Status of Animals

From Field Condition

Apparently normal, but animals had neurological symptoms of

different diseases

G-3. Overall progress of diagnosis of different neurological diseases with special reference to BSE/ TSE diagnosis & surveillance done by ERDDL :

G-4. Histopathological Interpretation of Bovine Brains:

Sl No.

Histopathological Observation No. of Brains Percentage of lesion (%)

Remarks

1. Total No of Brains collected 188 - Different Abattoirs & fields of West Bengal, Andaman &Nicobar Island

,Bihar,Orissa ,Sikkim 2. No. of brains screened 188 - - 3. Chronic encephalopathy 20 10.63 % Special emphasis should be given for

collection of brain samples from clinically suspected cases from Jharkhand as per GOI guideline, otherwise the target will not be completed in future

4. Vasculo encephalopathy 09 4.78 % 5. Rabiform encephalopathy 02 1.06 % 6. Bac./Viral/Toxic infective

encephalopathy 00

7. Low grade encephalopathy 42 22.34 % 8. Tumors/ cyst/ parasitic lesion 00 9. No significant lesion 115 61.17%

Sl.No. Parameter Total Source & Result 1. Total no. of brains collected 188 Abattoirs & Fields, 2. Brain collected from Abattoir 130 Abattoirs, 3. Brain sample from Field 58 Clinical sample from field 4. Number of brains processed ** **Brain samples are stored in -20°C deep freezer for

screening with BSE ELISA test kit (after procurement of the kit) as per recent OIE guideline. Vide ref no vide. No. K-11053/28/2016-LH of the Joint Commissioner (LH), Department of Animal Husbandry, Dairying & Fisheries, GOI, Krishi Bhaban, New Delhi Dated 20/03/2019.

6. Number of brain screened for histopathology

188 Negative to BSE hallmark lesion

7. Total number of brain send for screening of Listeriosis

186 Negative to Listeriosis

8. Brain examine for Rabies by Seller’s staining

58 Negative to Rabies

21 G-5. Surveillance Point values for samples collected from animals in the given Sub population & age Category in the Bovines of Eastern Region at ERDDL

G-6: Audit findings in feed mills producing feed for ruminants (inspections and sampling)

Type of plant

Number of plants Number of plants in (A) inspected

Total number of visual inspections in (B)

Total number of plants in (B) with infractions

Total number of inspected plants in (B) with sampling

Total number of plants in (C) with positive test results

(A) (B) (C) For ruminants only

Shri Krishna Goshala, Patna City, Bihar

01 01 Nil 01 (05)

Negative

Go Rakshini Sabha, Hajipur 01 01 Nil 01(02) Negative

V.N. Feed Mills, Birdline, Prothrapur, Port Blair

01 01 Nil 01(01)

Negative

Vinoth Feeds & Poultry Pvt. Ltd, Calicut, Port Blair

01 01 Nil 01 (01)

Negative

Pashupati, Agrovet Pvt. Ltd. 01 01 Nil 01(02) Negative

OMFED, Radhadamodarpur, Cuttack

01 01 Nil 01(02)

Negative

Samrat Feed Mill, Jalpaiguri 01 01 Nil 01(02) Negative

Siliguri Feed Plant, WBLDC 01 01 Nil 01(04) Negative

Kalyani Feed Plant, WBLDC 01 01 Nil 01(05) Negative

Salboni Feed Plant, WBLDC 01 01 Nil 01(03) Negative

Feed Mixing Plant, Salboni, PBGSBS

01 01 Nil 01(02)

Negative

Durgapur Feed Plant, WBLDC 01 01 Nil 01(04) Negative

Amul Dairy Plant, Jairambati Bankura

01 01 Nil 01(05) Negative

13 13 13(25)

For Multispeces

NIL

NIL NIL NIL NIL

Summary Table for BSE Surveillance

April 18 - March 19

Surveillance Subpopulation

Age Routine Slaughter

Fallen Stock Casualty Slaughter

Clinical Suspect

Total

Sample Points Sample

Points Sample Points Sample Points

>1&<2 yrs - - - - - - 00 00 00

>2&<4 yrs 00 00 - - - - 06 1560 1560

>4&<7 yrs 12 12x0.2=2.4 - - - - 42 31500 31502.4

>7&<9 yrs 83 83x0.1=8.3 - - - - 07 1540 1548.3

>9 yrs 35 35x0=00 - - - - 03 135 135

Total 130 10.7 - - - - 58 34735 34745.7

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H. Immune status studies on Ranikhet Disease in vaccinated organized and backyard poultry population in the region (as per technical programme 8) :

To study immune status in organized poultry farms following scheduled vaccination against Ranikhet disease as well as sero survey work against Pullorum disease were conducted during the period. A total of 3674 no. of serum samples were received from backyard and organized farms of West Bengal, out of which 2298 (67.17 %) samples exhibited protectivity against Ranikhet disease.

H-1. Results of serum antibody against Ranikhet & Pullorum diseases:

Source of sample

No. of Sera

sample collected

No. of sera sample tested

Test Performed

Result for Ranikhet Disease

Result of Serological test for S. pullorum

Protective

(>1:32)

Non-protective(<1:

16)

West Bengal

(Backyard and organized Farm)

3671

3671

HA, HI

2298 (67.17 %)

1376

(32.83 %)

24 samples were shown sero-positive (0.57 %) for detection of Salmonella Pullorum out of 4211

samples tested A&N 3 3

TOTAL 3674 3674

The absence of development of protective titer to the desired level among immunized birds for R.D. Immune Status Studies may be due to various reasons. The improper maintenance of cold chain, presence of immune suppressive factors (as IBD infection) or other consistent infection may play some role in it.

Table : 5 No. Of Sample Collected For RD Immune Status Studies during last ten years

2009-10 2010-11

2011-12

2012-13

2013-14

2014-15

2015-16

2016-17

2017-18

2018-19

2572 4148 3892 4139 4260 3652 4018 3288 3437 3674

Serum sample tested for RD immune Status Studies During last ten years

23

I. Routine sero-surveillance work and Antigen detection in swab / tissue samples of HPAI in the region (as per technical programme-11) :

Avian Influenza Laboratory was established to detect an area affected with Avian Influenza in quickest possible time to prevent further spread, also to carry out routine surveillance diagnostic work of Avian Influenza in different states under jurisdiction of RDDL(ER) with the intention to verify that an area is free from Avian Influenza infection or not.

I-1. Work undertaken and Reports during the period:

i. Receiving, cataloguing, pooling and numbering of samples for surveillance of Avian

Influenza.

ii. Serological examination of samples by competitive ELISA for detection of Influenza virus

Type A antibody.

iii. Serological examination of sera samples positive for Influenza Type A viral antibody by

Haemagglutination Inhibition (HI) Test for detection of Influenza virus Subtype

H5/H7/NDV etc. antibody.

iv. Diagnosis of randomly selected swab / tissue surveillance samples and detection of viral

antigens of Influenza virus Type A by Immuno-chromatographic Test (Rapid AIV Test kit).

v. Examination of swab / tissue surveillance samples for Avian Influenza Virus in

Embryonated Chicken Eggs (ECE) and HA test.

vi. Detection of Avian Influenza viral nucleic acid by Real Time RT PCR test in suspected

swab / tissue samples of unusual mortality among poultry birds.

vii. Conventional RT-PCR test for detection of Avian Influenza virus if necessary.

viii. Preparation of logistics for sample collection like sample transport media, dispensing and

distribution of logistics for serum and swab collection on indent in regular basis.

24

A total of 48936 numbers of samples were received in the Laboratory form

different states for routine surveillance diagnosis during the period 2018-19. Sera

Samples received for Surveillance from different states are 9622 (5941, 2751 and 930

sera from fowls, ducks and live bird markets respectively) from West Bengal, 2604 from

Odisha, 2519 from different districts of Bihar, 77 from Jharkhand and 490 from A.N.

Islands. No sera samples were received from Sikkim. Morbid Samples {cloacal swab,

tracheal swab, faecal samples from migratory birds, environmental samples (drinking

water, waste water, droppings, processing tables, processing knives etc)} that were

received for Surveillance of Avian Influenza during the period are 9851 from West

Bengal, 18153 from Odisha, 2130 from Jharkhand, 3250 from Bihar, 240 from Andaman

& Nicobar Islands.

Different districts of West Bengal have sent 19473 total number of samples

(Annexure II) of which Howrah has sent 7608 samples , North 24 Pgs – 1405, South 24

Paraganas - 1648, Kolkata -16, Murshidabad - 288, Nadia - 23, Hooghly- 598, Midnapur

East -1859, Midnapur west- 766, Jhargram- 139, Bankura - 693, Purba Burdwan - 858,

Paschim Burdwan- 116, Birbhum- 140, Purulia - 60, Malda - 20, Uttar Dinajpur - 228,

Dakshin Dinajpur-1506, Coochbehar-1416, Jalpaiguri-28, Darjeeling-nil, Kalimpong- 58.

Unusual mortality samples have been received 48 no. from Bihar.

Avian influenza surveillance programme has been done and will be continued in

future as per national surveillance plan provided from DADF, Govt. of India. A special

emphasis has been given on collection and testing of samples from the hot-spot areas viz

the national and international border areas, areas of previous occurrence, water bodies

and wild life sanctuaries and specially the Live Bird Markets (LBM). A total of 1855

samples collected from LBM (Ann-III) from different states of which the number of sera

and morbid samples were 930 and 925 respectively. In addition to it, 6723 environmental

samples and 448 faecal samples from migratory birds were tested in the laboratory.

The different tests undertaken in the laboratory during the period of April 2018 to

March 2019 for examination of the sera samples were Competitive ELISA and

Hamagglutination Inhibition tests. Of these, AIV Ab ELISA was done for detecting

Avian Influenza Type A antibody in sera samples (Annexure IV) while Hamagglutination

Inhibition test (HI test) using H5/H7/H9/NDV Subtype specific antigen for detecting

Avian Influenza virus subtype specific antibody in sera samples (Annexure V). Though

no samples were found positive by ELISA, a fraction of them were further tested by HI

test for cross checking. A total of 15312 and 3830 sera samples were tested by ELISA

and HI test respectively as can be seen in Annexure IV and V respectively.

In respect of examination of routine morbid samples, Embryonated Egg

Inoculation technique (Annexure-VI) has been done in routine basis and ‘Egg Inoculation

test’ followed by Slide agglutination test and if necessary, Hamagglutination Inhibition

test using H5/H7/H9/NDV specific antisera has been carried out. It was observed that the

Chromatographic Immunoassay using Anigen® Rapid AIV Ag test kit (Annexure-VII)

has a fast and fairly acceptable detection limit. Therefore, initial screening of the morbid

samples by this kit can reduce handling of the large number of embryonated eggs. This

may allow the laboratory worker to devote more time and energy for subsequent

virological examination of the eggs after inoculation with only those samples that

exhibited positivity in Chromatographic Immunoassay. However, this will need enough

number of Chromatographic Immunoassay kits (Rapid AIV Ag test kits) in the year

2019-20. But, Chromatographic Immunoassay too has its limitation as it exhibits

positivity irrespective of virus survivability.

For examination of morbid samples of unusual mortality, ‘Real Time RT-PCR

test’ can be carried out as a quick and confirmatory test for detection of viral nucleic acid

of Avian Influenza virus. About 851 samples were tested by Real Time RT-PCR this year

(Annexure VIII) and were confirmed negative for Routine Surveillance sample. Among

48 samples from unusual mortality 6 no. were positive for highly pathogenic H5 Avian

Influenza by chromatographic Immunoassay, Real Time RT PCR & other technique

(Annexure IX).This proves that the laboratory is ready to handle any unusual mortality

samples suspected of HPAI. This proves that it was worth establishing the Pre-Fabricated

BSL-III laboratory and its relevant instruments. The activities performed in the laboratory during the period under reporting are tabulated here :

I-2. Details of fit samples received in Avian Influenza Laboratory:

I-3. Details of Live Bird Market samples and Environmental Samples received and tested:

State

Environmental

samples Faecal

sample

Migratory

/zoo birds

Live Bird Market (LBM)

Backyard Zoo

birds Serum Morbid

A&N Islands 33 0 0 0 33 Bihar 972 57 0 0 972 Jharkhand 0 0 0 0 0 Odisha 3890 12 0 0 3890 Sikkim 0 0 0 0 0 WB 1828 379 930 925 1828

Total 6723 448 930 925 6723

State Serum Cloacal Swab Tracheal Swab Env

samples F/S Mig

birds Live Bird Market (LBM)

Total samples received

Fowl Duck Fowl Duck Fowl Duck Serum Morbid

A&N Islnd 490 0 125 0 82 0 33 0 0 0 730

Bihar 2519 0 1287 5 912 17 972 57 0 0 5769

Jharkhand 77 0 1069 30 1009 22 0 0 0 0 2207

Orissa 2604 0 7173 0 7078 0 3890 12 0 0 20757

Tripura 0 0 0 0 0 0 0 0 0 0 0

Sikkim 0 0 0 0 0 0 0 0 0 0 0

WB 5941 2751 2647 1716 2283 73 1828 379 930 925 19473

Total 11631 2751 12301 1751 11364 112 6723 448 930 925 48936

Env samples= Environmental samples, F/S Mig birds= Faecal samples of Migratory birds

26

I-4. Details of Serum Samples tested by ELISA:

State Fowl sera

samples

No. of

positive

fowl sera

samples

Duck sera

samples

No. of

positive

duck sera

samples

Live Bird

Market(LBM)

samples

No. of

positive

LBM sera

samples

Total sera

samples

tested

A & N Islands 490 - - - - - 490

Bihar 2519 - - - - - 2519

Jharkhand 77 - - - - - 77

Odisha 2604 - - - - - 2604

Sikkim 0 - - - - - 0

West Bengal 5941 - 2751 - 930 - 5941

Total 11631 - 2751 - 930 - 11631

I-5. Details of Serum samples tested by Haemaglutination Inhibition test : State Fowl sera

samples

No. of

positive

fowl sera

samples

Duck sera

samples

No. of

positive

duck sera

samples

Live Bird

Market(LBM)

samples

No. of

positive

LBM sera

samples

Total sera

samples

tested

Andaman &

Nicobar

Islands

98 - - - - - 98

Bihar 636 - - - - - 636

Jharkhand 24 24

Odisha 637 - - - - - 637

Sikkim - - - - - -

West Bengal 1190 - 1266 - 199 - 2655

Total 2585 - 1266 - 199 - 4050

I-6. Details of Morbid samples (swab/tissue etc.) tested by Embryonated Egg Inoculation

Technique: State Backyard &

Organised

sector

samples

Environmental

samples

Live Bird

Market

samples

Faecal

samples of

Migratory

Birds

Total

samples

tested

Total

samples

found

positive

Andaman & Nicobar

Islands

207 33 - - 240 -

Bihar 2221 972 - 57 3250 -

Jharkhand 2130 0 2130

Odisha 14251 3890 - 12 18153 -

Sikkim - - 0 -

West Bengal 6719 1828 925 379 9851 -

Total 25528 6723 925 448 33624 -

27 I-7. Details of Swab / tissue samples / Harvested Allantoic Fluid tested by

Chromatographic Immunoassay:

State Total Morbid samples tested

Total samples found positive

Andaman &

Nicobar Islands 20 -

Bihar 270 -

Jharkhand 175

Odisha 1500 -

Sikkim -

West Bengal 807 -

Total 2772 -

I-8. Morbid samples/ Harvested Allantoic fluid samples tested by ‘Real Time RT-PCR’

State Total Morbid samples

tested

Total samples found

positive

Andaman & Nicobar Islands

6 -

Bihar 81 -

Jharkhand 54

Odisha 461 -

Sikkim

West Bengal 249 -

Total 851 -

I-9. Unusual mortality samples tested by different technique

State Total no. samples tested

Total samples found positive

Chromatographic

Immunoassay’

Total samples

found

positive

Real Time

RT-PCR’

Samples tested by

‘Embryonated

Egg Inoculation

Technique’

HI Test against H5

AIV

antisera

Andaman &

Nicobar Islands

-

Bihar 46 6 6 6 6

Jharkhand -

Odisha - -

Sikkim 2 - -

West Bengal - -

Total 48 6 6 6 6

28

J. Proximate Analysis of Animal and Poultry feed/feed ingredients (as per technical programme-13):

Proximate Analysis Lab. at IAH &VB (R&T) Kolkata is engaged in the analysis of various feed and feed ingredients for various parameters like moisture, ash, fibre, ether extract etc. as per requirement of the farmers/farms. Forty seven feed samples from West Bengal were received for proximate analysis during the period; as follows:

Feed No. of samples

tested Below the Reference

Parameter included

Cattle Feed 15 1 Moisture %, Crude Protein %, Ether Extract%, Crude Fibre %, Total ash%

Poultry Feed 31 2

Rabbit Feed 01 0

TOTAL 47 03

J-1 Detection of MBM from feed mill samples: Thirty eight samples were received for presence of MBM in cattle feed during the period; as follows:

K. To develop analytical facilities of pesticides, antibiotics and chemical residues in

Livestock & poultry products (as per technical programme-14) :

As per mandate of ERDDL, pesticides, antibiotic and drug residues in livestock and poultry products are to be determined. For the purpose, a HPLC laboratory has been established. Now the laboratory is functioning with the following activities.

K-1. Standardization of Pesticides:

Standardization of the pesticides like Cypermethrin, Deltamethrin, Endosulfon, BHC, Quinalphos, Malathion, Monocrotophos, Butachlor, Imidacloprid and Phosphomidon has been done.

States Name of the feed mill No. of

samples

Sample

positive

Sample

Negative Remarks

West

Bengal

Salboni Feed Plant, WBLDC 03 00 03

Salboni Feed Mixing Plant, PBGSBS 02 00 02

Amul Feed Plant, Jairambati, Bankura 05 00 05

Durgapur Feed Plant, WBLDC 04 00 04

Kalyani Feed Plant, WBLDC 05 00 05

Siliguri Feed Plant, WBLDC 04 00 04

Samrat Feed Plant, Siliguri 02 00 02

Bihar Shri Krishna Goshala, Patna City, Bihar 05 00 00

Go Rakshini Sabha, Hajipur 02 00 00

A & N V.N. Feed Mills, Birdline, Prothrapur, Port Blair 01 00 00

Vinoth Feeds & Poultry Pvt. Ltd, Calicut, Port Blair 01 00 00

Odisha Pashupati, Agrovet Pvt. Ltd. 02 00 00

Omfed, Radhadamodarpur, Cuttack 02 00 02

Total 38 00 38

29 K-2. Standardization of Mycotoxins:

Standardization of Mycotoxins like Aflatoxin-B1, B2, G1, G2 and Ochratoxin has also been standardized.

K-2. Standardization of Antibiotics:

Two antibiotics Oxytetracycline and Ceftriaxone have been standardized.

K-4. Sample analysis :

One Fifty Six (156) tissue samples for analysis of pesticides and herbicides; and Sixtyone (61) feed samples for mycotoxin analysis has been done during the period. Sample analysis report is as follows:

L. Study of Nutritional Status in infertility cases (as per technical programme-15) : Biochemistry and Toxicology Lab. of RDDL (ER), Kolkata performed Biochemical tests for 744 samples, Heamatological tests for 50 samples and detection of adulteration

(synthetic egg) for 08 egg samples & 226 meat samples received from West Bengal during the period.

L-1 Biochemical Tests performed

Species

Total

samples

tested

Parameters

Total

Protein Albumin Calcium Phosphorus

Bovine 744 744 744 744 744

Poultry 20 20 20 20 20

Total 764 764 764 764 764

Nature of Sample

Test Done

Source of Samples No. of Samples

tested

Analytical Results

Positive (beyond

permissible limit)

Negative (Nil / within

permissible limit)

Feed

materials

Mycotoxin

Analysis

West Bengal 52 8 44

OTHER STATES

Odisha 09(cattle-8, duck-1)

1duck 08

TOTAL 61 9 52

Tissue

samples

Pesticide

Residue

Analysis

West Bengal 136 22 114

OTHER STATES

Bihar 04 0 04

Odisha 16 0 16

TOTAL 156 22 134

30 L-2 Biochemical Tests performed from serum samples of Canine

Species Total

tested

Parameters

Total protein Albumin Creatinine BUN SGOT SGPT

Dog 10 10 10 10 10 10 10

Tiger 6 6 6 6 6 6 6

L-3 Haematological tests performed

Species Total samples

Tested Hb% TLC DLC

Cattle 5 5 5 5

Poultry 18 18 18 18

Dog 5 5 5 5

Tiger 6 6 6 6

L-4 Detection of adulteration (synthetic egg/ meat)

M.Soil testing of Anthrax Spore in the Endemic area (as per technical programme- 16) :

M-1. Screening of soil samples for B. anthracis: Soil samples were received from different parts of the state & outside of the state from

the surface of the soil contaminated by the terminal discharge of the suspected animals or from endemic zone of anthrax and processed for isolation of B. anthracis spores as per the methods of OIE.Four soil samples were received from Burdwan, South 24-pgs, Murshidabad districts of West Bengal. All samples were found negative to anthrax.

M-2. Details of soil sample processed:

N.

Species Poultry Cattle Goat Sheep Pig

Meat 226 22 63 1 5

Egg 08 - - - -

Sl. No.

Source of Samples

No. of Samples Received

Positive

Negative

WEST BENGAL

1. Bainchi, Pandua Block, Hooghly 1 1 0

2. Sitai Block, Coochbehar 1 0 1

3. Hariharpara Block, Murshidabad 2 0 2

TOTAL 4 1 3

Screening of Export / Import Materials for B. anthracis :

(as per technical programme-17)

A total 188 nos. of sample were received from different sources through Regional officer (ER), Animal Quarantine and Certification Services, NSCBI Airport, Kolkata for testing. All the samples were tested as per the methods of OIE using specific media. All of 188 no. of samples were negative to Bacillus anthracis.

O. Maintenance of repository of infective agents isolated from the Eastern Region (as per technical programme-18) :

Work has been undertaken to maintain different bacterial and viral agents in this laboratory as State Repository. Following infectious agents so far isolated and identified are maintained as state repository as per mandate of RDDL.

O-1. Maintenance of Bacterial agents:

A good no. (494) of bacterial isolates from different clinical and environmental samples are maintained by subculture in artificial media in this section as State Repository.

Sl. No.

Name of Bacterial Agent

Maintained by Isolated From

Routine Subculture In Artificial Media

No. of isolate

01 Typed Escherichia coli -Do- 38 Poultry, Love bird, Goat, Pig, Rabbit, Garole Sheep etc.

02 Escherichia coli -Do- 135 Poultry, Pheasant, Black Swan, Badrika, Dog, Garole,

Sheep, Cocktail, Bovine, Ostrich, Kangaroo, Tiger, Rabbit, Spoonbill, Swamp Deer, Wild Elephant, Lengur etc.

03 Screened Escherichia coli -Do- 22 Poultry

04 Escherichia coli -Do- 35 Solely from wild animals

05 Typed Salmonella sp. - Do - 30 Poultry , Cat, bovine etc.

06 Salmonella sp. -Do- 52 Poultry, bovine, caprine, etc.

07 Proteus -Do- 19 Dog, poultry, Rabbit, etc.

08 Pasteurella multocida - Do - 29 Duck, Cattle , Buffalo, Poultry

09 Pasteurella haemolytica - Do - 01 Pin tailed duck

10 Bacillus anthracis - Do - 48 Cattle, Goat, Soil.

11 Pseudomonas sp. - Do - 30 Dog, Pig, Poultry.

12 Streptococcus sp. - Do - 08 Milk.

13 Staphylococcus sp. - Do - 41 Horse, Tiger, Deer, cattle etc.

14 Listeria monocytogenes - DO - 02 American Type Culture Collection (ATCC), Bovine etc.

15 Staphylococcus aureus

(Cowan Strain) - Do - 02 Microbial Type Culture Collection, Chandigarh.

16 Streptococcus agalactiae - Do- 01 ATCC

17 Salmonella pullorum St.-17 -Do- 01 Avian

TOTAL 494

32

O-2. Maintenance of Viral agents:

In our laboratory we are maintaining the following viral agents isolated from poultry and duck origin using Embryonated eggs.

P. Organizing training programme for veterinary & para-veterinary work force (as per technical programme -19) :

One such programme was organized during the period; as follows:

Held On Training

/workshop On Participants from Infectious Agents/Disease Covered

30th.

November

-

1st.Decem

ber, 2018

COMMON DIAGNOSTIC TECHNIQUE

FOR DISEASES OF LIVESTOCK

All the states under jurisdiction

of RDDL (ER)

Pasteurella multocida, Bacillus anthracis

Brucella sp., Salmonella sp. E.coli,

Listeria sp., Clostridium chauvoei

Mycobacterium tuberculosis

NDV, IBDV, IBV, Trypanosoma sp.

Babesia sp., Theileria sp.,Anaplasma sp. etc.

Q. Detection of Microbial causes responsible for clinical and sub- clinical Mastitis and its anti-biogram (as per technical programme-20):

To identify the early and latest cases of Mastitis and causal agent, Mastitis Control Unit of this Institute received/collected 117 samples from different organized and private farms. A total of 111 samples showed positive. The test results are depicted in the following table:

Total sample received/ collected

Sample tested

Result Organisms Isolated

Staphylo- coccus sp.

E. Coli Ps.

aeruginosa T

OT

AL

+ ve - ve

117 117 111 6 51

(42.50%) 57

(47.5%) 12

(10.0%) 120

Sl No. Name of viral agent Isolated from No of isolates Maintained in

01 RDV Poultry 56 Hens’ embryo 02 IBDV Poultry 29 Hens’ embryo

03 IBV Poultry 33 Hens’ embryo

04 MDV Poultry 1 Hens’ embryo

02 DPV Duck 4 Ducks ’embryo

TOTAL 123

33

R. Monitoring of the state sponsored schemes on Disease Investigation (as per technical programme-21) :

R-1. P.M Examination of different Poultry birds:

Month Duck Quail Case birds

Pigeon

Fowl

Broiler Colour

Broiler*/ Kroiler

Turkey RIR Layer Guinea fowl

Kadaknath

Black Austrolop

ND*

April 02 02 75 02 30 04 02

May 06 01 31 02 07

June 01 20 03

July 46 02

Aug. 01 31 02 04

Sept. 03 21 10 16

Oct. 27 10

Nov. 01 01 01 04 03 20

Dec. 04 01 05 32 10 07

Jan. 02 03 02 64 01 21 04

Feb. 02 57 05 08 06 02

March 05 15 85 08 05 43 01 GF-1

SF-1

03 03 01

TOTAL 18 03 25 12 493 34 17 156 16 02 03 03 16

* Non-descriptive

R-2. P.M Examination of Small and Lab. animals:

Type of cases Small animals Laboratory animals

Month Pig Dog goat Rabbit Guinea-pig Mice

April 01 01 03

May 07 04

June 10

July 02 01 11

August 10

September 01

October

November 01 01

December 01 04

January 01 16 05

February 02 03

March 05 06

TOTAL 03 24 03 10 56

34 R-3. Necropsy (Post mortem) findings/Suggestive diseases of Poultry:

Type of P.M lesions Total

Brooder Pneumonia 20

IB & Pasteurella 19

Colibacillosis/ Colisepticaemia / Coli-granuloma 101

Ascites/hydropericardium 02

Coccidiosis 100

Coldness 17

ALV 01

Heat Stress / Severe Dehydration 02

Helminthiasis (Ascariasis, Capillariasis, Cestodeasis etc.) 04

IBD / Scl. IBD 32

IB 102

Wing rot 06

Ranikhet Disease 114

Salmonellosis 02

IB&IBH 19

Visceral Gout / Articular Gout 16

ND&pasteurella 02

Pseudomonas 02

Avian Malaria 06

E.coli & RD 01

IBH 59

Fowl pox 06

Omphalitis 08

Others 74

R-4. Wildlife samples for post-mortem examination:

Sl. No.

Type/Species of Wild animals /birds

PM done (Nos.)

P.M. and Lab. observation

WILD BIRDS

1 Crow 03 NSO

2 Love Bird 09

,E.coli,Capillaria sp., IBD, Pox,Staphylococcus sp.,liver disfunction.

3 Budgerigar 01 Staphylococcus sp

4 Coppersmith barbet 03

5 Finch 15 E. coli

6 Indian parakeet 02 Putrified

7 Pigeon 11 Haemoproteus columbae, E.coli,Capillaria sp, Coccidia,

8 Black kite 02

9 Cokabura loughing kingfisher(Australian King Fisher)

01

10 Peacock 01

11 Reeves Pheasant 01

12 Bulbul 01

13 Migratory birds 02

REPTILES, WILD ANIMALS

Samples not received

35 R-5. Postmortem Materials & isolates sent/analyzed at different laboratories:

R-6. Month wise Samples Received for Laboratory Examination at BTTCU :

Sl. No.

Type of Material Sent/ Cultured

Name of the Section Agent Identified / Findings

(Report as received)

1.

PM Materials / Bacterial isolates / Impression Smear

& Blood Slide

RLBD Lab.* E.coli.- 103 Salmonella sp.- 02

Staphyllococcus-06 ,Pasteurella sp.- 02 Clostridium sp.-20, Pseudomonas-02

2. PM Materials

RLBD Lab.* / Diag. Virology Lab., ERDDL

New Castle Disease Virus (NDV) – 114, Infectious Bursal Disease Virus (IBDV) - 32

Infectious Bronchitis Virus (IBV) – 108 Aspergillus – 20

PPR-7,Swine Fever-1, Goat pox-1

3. Tissue materials for

HP

Diag. Path. Lab. Positive findings according to HP study : (a)Pneumonia-3 (b) Electrocution-01

4. Tissue materials HPLC Lab. Poisoining-04

5. Intestinal

scrapping/content

Diagnostic Parasitology, RDDL

Eimeria sp.- 112 Cappilaria sp.- 04

Tape worm-06 Haemoproteus columbae-05

Echinostoma- 01

Month

& Year

Types of sample received Total

Blood slides

Lymph gland

smear

Faecal sample

Intstinal scrapping

Skin scrap.

Ecto-parasite

Serum Whole Blood

Whole worm

April’18 79 - 5 53 - - - 1 - 138

May’18 103 - 7 38 - 20 - 37 - 205

June’18 174 - 13 19 - - - 8 - 214

July’18 8 - 29 36 - - - 10 - 83

Aug’18 143 - 20 21 - - - 1 - 185

Sept’18 451 - 9 41 1 - - 3 - 505

Oct’18 86 - 7 19 - - - - - 112

Nov’18 79 1 3 9 - - 70 16 - 178

Dec’18 104 1 26 44 - - - 3 - 178

Jan’19 76 - 16 51 - - - 3 - 146

Feb’19 66 - 30 27 - - - 2 10 135

March’19 61 - 2 106 - - - 6 - 175

Total 1430 2 167 464 1 20 70 90 10 2254

36

R-7. Month wise Samples found positive at BTTCU:

Diagram of No. of samples positive for parasitic cases for the year 2018-19

Month

&

Year

Positive cases Total

Blood

slides

Lymph

gland

smear

Faecal

sample

Intstinal

scrapping

Skin

scrap.

Ecto-

parasite

Whole

Blood

Whole

worm

April’18 18 - 2 17 - - - - 37

May’18 29 - 2 9 - 20 10 - 70

June’18 54 - 9 8 - - 2 - 73

July’18 34 - 24 5 - - - - 63

Aug’18 63 - 9 12 - - - - 84

Sept’18 87 - 8 10 - - - 105

Oct’18 23 - 2 6 - - - - 31

Nov’18 47 1 1 2 - - 7 - 58

Dec’18 34 - 12 18 - - 1 - 65

Jan’19 24 - 3 28 - - - - 55

Feb’19 21 - 9 10 - - - 10 50

March’19 22 - 1 26 - - 3 - 52

Total 456 1 82 151 - 20 23 10 743

37 R-8. Type of Parasites identified at BTTCU:

(A) Haemoprotozoan parasite/Haemoparasites (B) Eggs/ova of Helminths

(C) Oocyst of Protozoa (D) Ticks/ Mites

(E) Whole worm

S. Monitoring of Centrally sponsored scheme like NPRE and State ELISA Laboratory (as per technical programme-22 ) :

S-a. Report of Paste-Des-Petits Ruminants (PPR) Diagnostic Laboratory :

Established in 1997, the State ELISA Laboratory of Institute of Animal Health & Veterinary Biologicals (R&T) has been providing confirmatory diagnosis for Peste-des-Petits Ruminants (PPR), a highly infectious disease of sheep and goats. During the period total 181 nos. samples were tested for antigen and 119 nos. samples were found positive. Samples were collected as Nasal Samples, Ocular Samples, Buccal Samples, Rectal Swab and Post Mortem materials.

Sl.

No.

Haemoparasite Animal

infected

1 A. marginale Cattle & Goat

2 Theileria spp. Cattle & Goat

3 Babesia bigemina C/B Cattlle

4 Trypanosoma evansi Cattle/Buffalo

5 Babesia gibsoni Dog

6 Babesia canis vogeli Dog

7 Ehrlichia canis Dog

8 Haemoproteus columbae Pigeon

9 Candidatus Mycoplasma sp.

( Haemobartonella sp.)

Big Cat & cat

Sl.

No.

Name of Helminthic

eggs/ova

Animal/ Birds infected

1 Strongyle Cattle, sheep, goat

2 Paramphistomum sp. Cattle, Goat

3 Fasciola sp. Cattle

4 Monezia sp. Goat

5 Trichuris sp. Goat, Rh. Monkey &

langur

6 Strongyloides sp. Cattle, Goat, Dog,

Rh. Monkey & langur

7 Toxocara vitulorum Cattle calf

8 Toxocara sp. Big cat

9 Spirometra Spp. Fishing cat

10 Ancylostoma sp. Big cat

11 Ascaridia galli Poultry

12 Capillaria sp. Pigeon/Crow/peacock

13 Raillietina sp. Pigeon/Crow

14 Heterikis gallinarm Poultry

Sl.

No.

Name of Protozoan

Oocyst/ Cyst

Animal/ Birds infected

1 Oocyst of Eimeria sp. Poultry, Cattle calf,

Rabbit, Goat

2 Cyst of Balantidium coli

Cattle/Pig

Sl.

No.

Name of Tick/ Lice Animal infected

1 Boophilus

(Rhipicephalus) Sp.

Cattle

2 Hyalomma anatolicum

anatolicum

Cattle

3 Haemaphysalis sp. Cattle

4 Linognathus sp. Cattle

Sl.

No.

Name of Helminth Animal/ Birds infected

1 Paranoplocephala

mamillana

Rhinoceros

38

S.a-1. Diagnosis of Peste des Petits Ruminants (PPR) at a glance:

S. a-2. Outbreak & Samples Receipt Pattern of PPR in different Districts of West Bengal during the period :

Particulars No. No. of outbreaks for which samples turned positive to PPR 21

Total no. of samples tested for antigen 181 Total no. of samples found positive to PPR 119

Total no. of districts of West Bengal in which PPR was documented 8 Total no. of PPR samples received only in West Bengal 82

Total no. of samples found positive to PPR only in West Bengal 53 Total no. of outbreak reported from West Bengal 11

Total no. of PPR samples tested from outside of West Bengal 99 Total no. of samples found positive to PPR from outside of West Bengal 66

Total no. of outbreak reported from outside of West Bengal 10 Total no. of serum samples screened for antibody to PPR virus 3099

Total no. of post vaccination serum samples screened for antibody to PPR virus in West Bengal

2825

Total no. of post vaccination serum samples found positive for antibody to PPR virus

2641

Sl. No.

Name of Districts No. of

samples received

No. of positive samples

Suspected outbreaks

Confirmed outbreaks

1. Bankura 11 8 2 1

2. Birbhum No samples received

3. Purba Burdwan No samples received

4. Cooch Behar No samples received

No samples received

5. Dakshin Dinajpur 7 7 1 1

6. Darjeeling& Kalimpong No samples received

7. Hooghly 10 0 2 0

8. Howrah 4 0 1 0

9. Jalpaiguri 2 1 2 1

10. Kolkata 6 0 2 0

11. Malda No samples received 12. Murshidabad No samples received 13. Nadia 2 2 1 1

14. North 24 Parganas 13 12 4 3

15. Purba Medinipur 10 6 1 1

16. Paschim Medinipur No samples received 17. Purulia 16 16 2 2

18. South 24 Parganas Parganas Parganas

1 1 1 1

19. Uttar Dinajpur No samples received

20. Alipurduar No samples received

21. Jhargram No samples received

22. Paschim Burdwan No samples received

TOTAL 82 53 19 11

39

S.a-3. Seasonal outbreak Pattern of PPR in the region during the period:

Month Suspected PPR

outbreaks

reported within

WB

Within WB,

Positive

outbreaks

Outside state

Positive

outbreaks

Name of the

state

April 5 4 1 Bihar

May 3 2 0 -

June 0 0 1 Sikkim

July 2 0 3 Bihar(1),

Odisha(2) August 2 1 0 -

September 1 1 0 -

October 0 0 1 Bihar

November 0 0 1 Odisha

December 2 1 0 -

January 0 0 0 -

February 1 0 1 Bihar

March 3 2 2 Bihar

Total 19 11 10

S.a-4. Seasonal Outbreak Pattern of PPR in West Bengal during the period:

The diagrammatic representation on the matter is as follows:

40

S.a-5. Outbreak Pattern of PPR in West Bengal during last eight years:

S.b. National Project on Rinderpest Eradication work in West Bengal during the period:

Sl.

No.

Name of District Village

search

target

village

search

achieved

Percenta

ge

achieve

d

Day book

inspection

target

Day book

inspection

achieved

Percentage

achieved

1. Alipurduar 294 210 71.43% 270 192 71.11%

2. Bankura 3540 3428 96.84% 1175 1108 94.3%

3. Birbhum 2229 2229 100% 1050 1050 100%

4. Purba& Paschim

Burdwan

2570 2329 90.62% 975 679 69.64%

5. Cooch Behar 1139 1106 97.10% 625 875 140%

6. Dakshin Dinajpur 1486 1408 90.91% 725 638 88%

7. Darjeeling &

Kalimpong

656 656 100% 525 362 68.95%

8. Hooghly 1899 1823 95.98% 975 852 87.38%

9. Howrah 755 755 100% 700 700 100%

10. Jalpaiguri 442 442 100% 405 405 100%

11. Jhargram 1472 1472 100% 250 267 106.8%

12. Malda 1615 1615 100% 925 925 100%

13. Murshidabad 1927 1927 100% 1100 1100 100%

14. Nadia 1255 1144 91.15% 950 892 93.89%

15. North 24

Parganas

1619 1518 93.76% 1100 1097 99.73%

16. Paschim

Medinipur

5888 5888 100% 1000 958 95.8%

17. Purba Medinipur 3053 2589 84.80% 950 867 91.26%

18. Purulia 2452 1732 70.63% 850 825 97.05%

19. South 24

Parganas

2125 2125 100% 1100 1100 100%

20. Uttar Dinajpur 1550 1550 100. % 725 643 88.69%

Total 37966 35946 94.68% 16375 15535 94.87%

41

S.c. Surveillance programme on Glanders :

Thirteen serum samples from horse were collected from West Bengal and sent to National Research Centre on Equine (NRCE), Hissar for sero-surveillance.

Source of Sample Type of Sample

No. of Sample Results

REMARKS Positive Negative

Dhabdhabi, Baruipur

Serum

2 0 2

Samples tested at

NRCE, Hissar Vivekananda Police

Academy , barrackpur 11 0 11

TOTAL 13 0 13

T. Screening of Listeria sp. from bovine brain samples (as per technical programme- 7):

A total 154 number of brain samples received through Specialist Pathologist, ERDDL,

Kolkata, which were tested for Listeria sp. The entire samples were processed after

cold enrichment and following standard methods. All the samples were found negative

for Listeria sp.

Type of Samples Source of Samples No. of Sample

Results

Positive Negative

Obex of Medulla of Bovine Brain

Specialist, Diagnostic Pathology, ERDDL, Kolkata

154 0 154

TOTAL 154 0 154

42 U.a. Other Virological work (including Molecular Biological Diagnosis) at a glance

(as per technical programme-9, 12)

Standardization of molecular biological diagnostic techniques for different pathogens of

livestock and poultry diseases other than Avian Influenza has been taken during the

year 2018-19.

Disease States from which samples received

Positive samples

Negative samples

Total samples

Test Done

ALC West Bengal 1 9 10 PCR followed by isolation

Avi pox West Bengal 1 3 4 PCR

Avi pox Bihar 2 0 2 PCR

BoHV-I West Bengal 710 7346 8056 qPCR

BoHV-I Bihar 2 599 601 qPCR

BoHV-I Odisha 97 1265 1362 qPCR

CDV West Bengal 4 5 9 Immuno chromatography, RT PCR

CDV A & N Islands 11 0 11 Immuno chromatography

CSFV Bihar 2 17 19 RT PCR,C-ELISA

CSFV Sikkim 17 2 19 RT PCR

CSFV West Bengal 1 5 6 RT PCR

DVE West Bengal 0 21 21 PCR

FePV West Bengal 0 2 2 PCR

Goat Pox

Odisha 10 19 29 PCR and AGPT

Goat Pox

West Bengal 0 1 1 PCR

Herpes virus

West Bengal 4 0 4 PCR

IBH West Bengal 34 49 83 PCR

JEV Bihar 8 2 10 ELISA test done at CDDL

JEV & Nipah

West Bengal 0 252 252 RTPCR and RT qPCR done at NIHSAD

NDV Odisha 0 2 2 RT PCR

Rabies West Bengal 1 0 1 Immuno chratography

RHD West Bengal 0 3 3 RT PCR done at NIHSAD

Total 905 9602 10507

43

U.b. Other Bacteriological work of Molecular Biological Diagnosis at a glance (as per technical programme-9) :

Different referral samples from different sources under jurisdiction of RDDL(ER) have been diagnosed during the year 2018-19. Moreover, Standardization of molecular biological diagnostic techniques for different pathogens of livestock diseases has also been taken during the year 2018-19.

Sl. No.

Source of samples

Type of Samples Test Done No. of Samples

tested

RESULT

Positive Negative

1.

West Bengal PM material

/milk/sputum

PCR for detection of Mycobacterium tuberculosis

genome 2 2 -

2. West Bengal PM material /

whole Blood

PCR for detection of Pasteurella multocida

genome

39 39 -

Odisha 34 - 34

3. West Bengal PM material

/Nasal Swab

PCR for detection of Mycoplasma SP.

17 3 14

Odisha 34 - 34

4. West Bengal PM material PCR for detection of Clostridium chauvoei

genome 6 6 -

5. West Bengal PM material PCR for detection of

Clostridium perfringens genome

5 5 -

6. West Bengal PM material PCR for detection of

Mycoplasma gallisepticum genome

10 5 5

7. Odisha PM material PCR for detection of Shigella sp. genome

20 20 -

TOTAL 167 80 87

U.c. Other Parasitological work of Molecular Biological Diagnosis at a glance (as per technical programme-10) :

Standardization of molecular biological diagnostic techniques for different parasites of livestock has been taken during the year 2018-19.

Sl.

No. Diagnostic technique

Haemo-parasite identified

Target Gene

Animal/ Birds infected

Source Result Total Sample

Positive Negative

1

Poly

mera

se C

hain

React

ion (

PCR)

Theileria sp. 18S rDNA

Cattle/Buffalo/Goat

WB 25 30 55

Sikkim 7 15 22

2 Anaplasma sp. Msp4 gene

Cattle/ Goat WB 7 48 55

Sikkim 0 22 22

3. Babesia gibsoni 18S rDNA

Dog WB 14 15 29

4. Ehrlichia canis 16S rDNA

Dog WB 17 12 29

5 Trypanosoma evansi

SAG Cattle/Buffalo/ Dog/horse/camel

WB 06 49 55

Sikkim 0 22 22

44

V. Physical achievement of ERDDL for the year 2018-19 at a glance:

Sl. No.

Source of the samples No. of samples

received No. of samples

tested

R E S U L T R e m a r k s

Positive Negative

VIROLOGICAL SAMPLES UNDER SPECIALIST, DIAGNOSTIC VIROLOGY

1 West Bengal 20098 20098 1450 18648

2 Bihar 3855 3855 6 3849

3 Odisha 19321 19321 276 19045

4 A & N Islands 324 324 0 324

5 Sikkim 19 19 0 19

6 Jharkhand 2130 2130 0 2130

TOTAL 45747 45747 1732 44015

SEROLOGICAL SAMPLES UNDER SPECIALIST, SERO-EPIDEMIOLOGY

1 West Bengal 25320 25865 835 18191 *For PPR, 2825 Samples Screened &93.49%

Protective, For RDISS, 3418

screened & 67% protective

2 Bihar 2629 2629 24 2605

3 Odisha 4242 4242 35 4207

4 A & N Islands 1438 1438 166 1272

5 Sikkim 69 69 4 665

6 Jharkhand 77 77 0 77

TOTAL 33775 33775 1064 27017

BACTERIOLOGICAL SAMPLES UNDER SPECIALIST, DIAGNOSTIC BACTERIOLOGY & MYCOLOGY

1 West Bengal 2529 2529 856 2381

2 Bihar 04 04 0 4

3 Odisha 04 04 0 4

4 Sikkim 00 00 0 0

TOTAL 2537 2537 856 2389

PARASITOLOGICAL SAMPLES UNDER SPECIALIST, DIAGNOSTIC PARASITOLOGY

1 West Bengal 2304 2304 736 1568

2 Bihar 00 00 00 00

3 Odisha 00 00 00 00

4 Sikkim 32 32 7 25

5 Jharkhand 00 00 00 00

TOTAL 2336 2336 743 1593

BIOCHEMICAL & TOXICOLOGICAL SAMPLES UNDER SPECIALIST, DIAGNOSTIC BIOCHEMISTRY & TOXICOLOGY

1 West Bengal 1419 1419 156 1263

2 Bihar 11 11 0 11

3 Odisha 29 29 1 28

4 A & N Islands 2 2 0 2

TOTAL 1461 1461 157 1304

PATHOLOGICALS SAMPLES UNDER SPECIALIST, DIAGNOSTIC PATHOLOGY

1 West Bengal 484+150*=634 634 *BSE-Brain Samples from clinical suspected cases and from slaughter house

2 Bihar 7+15* =22 15

3 Odisha 16* 16

4 A & N Islands 25+2*=27 27

5 Sikkim 12+5*=17 17 0 0

TOTAL 528+188*=716 716 - 188*

45 V.1 GLIMPSE OF SAMPLES HANDLED AT RDDL, ER, KOLKATA DURING 2018- 19

Sl. No.

Source of the samples

No. of samples received

No. of samples tested

REMARKS

1 West Bengal 52304 52304

2 Bihar 6521 6521

3 Odisha 23612 23612

4 A & N Islands 1791 1791

5 Sikkim 137 137

6 Jharkhand 2207 2207

TOTAL 86572 86572

V.2 DETAILS OF SAMPLES TESTED IN RDDL, ER, KOLKATA DURING THE LAST

NINE FINANCIAL YEARS

Sl. No.

Financial Year Number of Samples

Tested

1 2010-11 69240

2 2011-12 43796

3 2012-13 42406

4 2013-14 57448

5 2014-15 73530

6 2015-16 91609

7 2016-17 90943

8 2017-18 74767

9 2018-19 86572

46

47