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The world leader in serving science

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Dynamis™ Medium Introduction

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Gibco™ Dynamis™ Medium An excellent scale-up option for your CHO Cell Clones

The Power to achieve

higher titers The Power to start faster

The Power to streamline

scale-up

Is designed to provide the power to help you seamlessly scale-up

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Dynamis means “power” in Greek

Dynamis™ medium provides

the power to achieve higher titers, start faster, and

streamline scale-up for upstream process development

and bioprocessing.

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Dynamis™ medium enables highest titers at harvest

Glucose only fed-batch cultures show Dynamis medium achieved 74% higher titer compared to the next-best competitor,

with highest growth in titer from day 14 to day 21 at 30%

Internal Clone IgG Titer Comparison on Harvest Day

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Dynamis™ medium enables highest titers in fed-batch culture

Fed-batch cultures show up to 60% higher titers – customer clone

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Enabling a faster & more sustained start with Dynamis™ medium

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VC

D (

E6

ce

lls

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Culture time (days)

Internal IgG Clone Viable Cell Density

Dynamis™ Medium

ActiCHO P

BalanCD™ CHO Growth A Medium

Cellvento™ CHO-200 Medium

EX-CELL® CHOZN Medium

HyCell™ CHO Medium

PowerCHO™ 2

PowerCHO™-GS

Quick to reach 10E6 cells/mL and sustain that level days longer

™

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Dynamis™ Medium Enables Highest Viability (Internal IgG Clone)

Glucose only fed-batch cultures show higher and more sustained viabilities

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Pe

rce

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bile

Cell

s

Culture Time (Days)

DG44 Viability

Dynamis™ Medium

ActiCHO P

BalanCD™ CHO Growth A Medium

Cellvento™ CHO-200 Medium

EX-CELL® CHOZN Medium

HyCell™ CHO Medium

PowerCHO™ 2

PowerCHO™-GS

™

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AGT™ dry format simplicity helps streamline scale-up

AGT: Advanced Granulation Technology

Dynamis™ AGT dry format product offers these time saving benefits

Key Benefits

Auto pH: Just add Water

No Add backs Required; single part

Fast Dissolution and Mix Time

Dust Free Addition to Tank

Minimal Filtration Pressure Buildup

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Comparable performance is seen when moving from CD FortiCHO™ medium to Dynamis™ medium

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Culture Time (Days)

Dynamis AGT Medium - Internal IgG Clone Growth Comparison to CD FortiCHO Medium

Dynamis™ AGT™ Medium Lot 1

Dynamis™ AGT™ Medium Lot 2

CD FortiCHO™ Medium Lot 1

CD FortiCHO™ Medium Lot 2

CD FortiCHO™ Medium Lot 3

Dynamis medium provides a more scalable option

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Comparable performance is seen when moving from CD FortiCHO™ medium to Dynamis™ medium

Dynamis medium provides a more scalable option

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CD FortiCHO™ medium generated clones produce similarly in CD FortiCHO™ and Dynamis™ media

• IgG-producing clones isolated using standard Freedom CHO-S workflow (in

CD FortiCHO medium)

• Day 14 titers

• SFB is simple fed-batch, glucose feeding only per Freedom CHO-S Manual standard protocol

• FB is fed-batch culture using CD EfficientFeed C supplement for feeding without optimization of conditions

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Clone 1 Clone 2

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(mg

/L)

Freedom™ CHO-S™ Clones in SFB

CD FortiCHO

Dynamis

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Clone 1 Clone 2

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(mg

/L)

Freedom™ CHO-S™ Clones in FB

CD FortiCHO

Dynamis

Dynamis medium helps to provide a seamless transition to production

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CD FortiCHO™ medium generated clones are stable in CD FortiCHO™ and Dynamis™ media

• IgG-producing clones isolated using standard Freedom™ CHO-S™

workflow (in CD FortiCHO medium)

• Titer determined in stability cell train

-40%

-30%

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-10%

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10%

20%

30%

40%

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Tit

er

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lati

ve

to

Ea

rly (

%)

Time (Generations)

Clone 1 Stability

CD FortiCHO

Dynamis

-40%

-30%

-20%

-10%

0%

10%

20%

30%

40%

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Tit

er

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lati

ve

to

Ea

rly (

%)

Time (Generations)

Clone 2 Stability

CD FortiCHO

Dynamis

Dynamis medium does not alter clone stability

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There is no significant change in product quality moving from CD FortiCHO™ medium to Dynamis™ medium

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day11 - CD FortiCHO™

medium

day 11-Dynamis™

medium

day14 - CD FortiCHO™

medium

day 14-Dynamis™

medium

day17 - CD FortiCHO™

medium

day 17-Dynamis™

medium

% G

lyc

an

Sample Day - Condition

Medium Comparison - DG44 IgG Glycan Analysis

%G0F %G1F %G2F %Man5/A1F

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Dynamis™ medium for seamless process development scale-up

Dynamis™ medium provides: • The power to achieve higher titers

• The power to start faster

• The power to streamline scale-up

Catalog Numbers Available: • 1L AGT™ dry format – A2617504

• 10L AGT dry format – A2617501

• 100L AGT dry format – A2617502

• 10Kg AGT dry format – A2617503

www.lifetechnologies.com/dynamis

Transfer to Dynamis medium today! Caution: For manufacturing, processing or repacking.