Driving Innovation in a G lobal M arket

D io n e x

D I O N E X C O R P O R A T I O N

© 2003 Dionex Corporation LPN 1561 DNX-AR-03

DX-500

1 2

Dionex continues to strengthen the performance of the DX-500 Ion Chromatography (IC) System with the addition of new automation products, innovativetechnologies and design enhancements. New andimproved eluent delivery systems, automated eluentgeneration and intelligent chromatography data handling give you an ion chromatography system that is versatile, reliable, and accurate. All system operationsfrom eluent generation to sample preparation are automated, resulting in an integrated laboratory thatoperates faster and more efficiently.

High Performance

The DX-500 IC offers the highest level of performance of any IC system on the market today. Itssuperior sensitivity and reproducibility is unmatched.Significant developments like the improved eluentdelivery system of the GP50 pump, the advent of theEG40 Eluent Generator, and the introduction of theAutoSelect™ AS50 Autosampler make the DX-500 IC system a state-of-the-art ion chromatograph with superior analytical performance.

Superior Technology

The DX-500 system represents over two decades of Dionex research, innovation, and expertise in ion analysis. Our column technology is developed in response to our customers’ application needs and is optimized for use with Dionex ion chromatographysystems. Dionex suppressor technology, now in its fifth generation, is unmatched in terms of performanceand reliability. With our exclusive “Just Add Water” technology, which provides automatic eluent generation,Dionex has brought unprecedented power and convenience to ion analysis.

Sensitive and Accurate Results

Every chemist needs sensitive and accurate resultsover a wide concentration. The DX-500 IC system provides reproducible ultra-trace analyses andidentification of components in the most complicatedsample matrices. For applications with diverse levels of analytes, the DX-500 can monitor ions over four orders of linear range.

Application Flexibility

In need of detecting low levels of inorganic anionsand cations, organic acids, amines, and transition metals? How about anions in concentrated bases and cations in concentrated acids? All of these analyses, and more, can be performed with the versatile DX-500 IC system.

Dionex Ion Chromatography

LC20 Chromatography

Enclosure

LC30 Chromatography

Oven

LC25 Chromatography

Oven

EO1 Eluent/Solvent

OrganizerStandard or Microbore

IP25Isocratic Pump

GP50Gradient Pump

EG40 Eluent

Generator

ED40Electrochemical

Detector

CD20Conductivity

Detector

AD25�UV-Vis Absorbance

Detector

PeakNet Chromatography

Workstation

LC20 Chromatography

Enclosure

LC30 Chromatography

Oven

LC25 Chromatography

Oven

AS50 Compartments

AS50Autosampler

Thermal or Chromatography

AS40Autosampler

DX-500 IC System Module and Accessory Options

The DX-500 Ion Chro m a t o g r a p h

Fast

Separate, detect, identify, and quantify multiple

ions in a matter of minutes. With a DX-500 system every

stage of the analysis process is automated, providing

maximum laboratory efficiency.

Easy to Use

All of the DX-500 system modules and accessories

are designed for ease of use. Built-in user friendly

diagnostics and help screens; bright, easy-to-read front

panels, and logical menus facilitate user operation.

Innovative modules like the EG40 Eluent Generator

and AS50 Autosampler automate difficult or mundane

operations for further simplicity. Eliminate eluent

preparation and the handling of acids and bases

with the EG40, and use the AS50 to simplify sample

management and preparation.

Versatile

Perform a wide range of applications using multiple

modes of detection. DX-500 modules are engineered to

be interchangeable. Different configurations of columns

and detectors allow for any IC application to be run.

Automate ion chromatography analysis on the DX-500

system with intelligent autosamplers and powerful

PeakNet chromatography software. Expansion from

a single to dual channel system is often just the

addition of another pump.

3 4

Superior Technologies “Just Add Water” Technology

This breakthrough concept means that you do nothave to prepare eluent or regenerant solutions to doIC — you just add water.

The heart of “Just Add Water” is the combination of membrane based technologyand electrolysis. Using the EG40 EluentGenerator, eluent is created continuously,on-line, from water. In the SRS-ULTRA®,regenerant ions are produced fromrecycled eluent.

“Just Add Water” technology pro-vides IC results that are fast, easy, andpowerful. This technology is just oneexample of how Dionex continues to improve the field of ion analysis.

Suppressor Technology

Suppressors work by reducing the conductivity of the eluent, while enhancing the conductivity of theanalytes. This dramatically improves sensitivity, stabilityand dynamic range for anions and cations.

Dionex was the first company to introduce IC instrumentation utilizing chemical suppression; that was25 years ago. Since then, we have continued to lead theindustry in innovative suppression technology. Our firstsuppressors were made from ion exchange resins usedas a packed bed. Packed bed suppression allowed iso-cratic separations for samples with simple matrices. By

the mid 80’s IC application demands inspired the inven-tion of gradient capable, high capacity, continuously

regenerated, MicroMembrane® Suppressors for usewith high capacity columns. External regen-

erant pumps were eliminated by usingAutoRegen® devices to improve reliabili-

ty. In the early 1990’s, suppression wasmade even more powerful with a

technical breakthrough; the Self-Regenerating Suppressor® (SRS).The SRS was the first generation ofcontinuous, electrically regenerated

membrane-based suppressors, thatallowed demanding gradient and

high capacity separations. SRS technology madeIC easier because operation without external regenerantsolutions or pumps was now possible.

Today, Dionex has taken suppressor technology a step further, by improving sensitivity and reliabilitywith SRS-ULTRA suppressors. Many benchmarks in sup-pression technology have been set by Dionex over the last20 years, and you can be assured we will continue todevelop new suppression products and technologies inthe years to come.

Small et. al.

Publish First Suppressed Conductivity

IC Paper Packed Bed Suppression– Restricted to isocratic operation with low capacity columns– Required off-line regeneration and external regenerant pumps– Required external acid/base regeneration solutions

MicroMembrane Suppression– Expanded suppression to gradient operation– Expanded suppression to use high capacity columns– On-line, continuous regeneration

SRS-ULTRA– The ultimate in reliability and performance– 20 years of breakthroughs and refinements in suppression design

Self-Regenerating Suppression– Uses electrolysis of water to supply regenerant ions– No external acid/base solutions or pumps required– High capacity for all applications including gradient operation – Allowed use of high capacity columns– Plug-in and “Just Add Water”

Dionex Commercialized the First Packed Bed Suppressors

Fiber Suppressor AutoRegen®

2 mm MicroMembrane SRS-II

SRS-ULTRASRS-I AutoSuppression®

MicroMembrane® Suppressor

1975 1987 1997 2000199819851981 19921991

SRS-ULTRA suppressors provide superior performance

with excellent baseline stability and low detection

limits for gradient and isocratic separations.

EG40 Eluent Generator

The EG40 makes IC easier than ever by generatinghigh-purity IC eluents on-line using electrolysis ofdeionized water. On-line eluent generation for IC eliminates the eluent contaminants that cause gradientbaseline shifts, resulting in higher sensitivity, improvedresolution and better peak integration. The EG40 delivers outstanding eluent reproducibility, which provides excellent retention time stability. There are no chemicals to handle or reagents to prepare. The entire process is automated and uses only water.

Column Technology

Dionex offers a wide range of polymeric IC columnsfor various modes of separation including ion exchange,ion exclusion, ion pairing, and reversed phase. By using Dionex columns for your IC work, you are benefiting from many years of expertise in polymericresin technology and IC application experience.

All resins and columns are developed and producedby Dionex. Dionex IC columns are constructed from non-metallic, non-corrosive PEEK components to eliminate contamination due to metal corrosion. A widerange of low, medium, and high capacity columnsdesigned for fast analysis times or optimum resolutionof complex samples are available.

Hydroxide eluents produced by the EG40are free of carbonate interferences, and as a result baseline shift is eliminatedallowing for enhanced resolution and higher sensitivity.

14818

5 6

Expand your analytical capabilities by using multipledetectors configured on a single DX-500 IC system.Dionex detectors are designed with user friendly servicediagnostics and on-screen directions for easy cell calibration. All of our detectors provide digital signals and are autoranging when used with thePeakNet Chromatography Workstation. The modulardesign of the DX-500 IC allows for one or more of thefollowing detectors to be configured on a system.

CD20 Conductivity Detector

Detection techniques like colorimetry, nonsuppressedconductivity, and UV-Vis absorbance usually cannotobtain the low detection limits and signal-to-noise performance that can be achieved using the DX-500 ICsystem with suppressed conductivity detection. TheCD20 is a microprocessor-driven precision conductivitydetector. Digital autoranging and temperature com-pensation combined with built-in control for the SRS Self-Regenerating Suppressors provide unsurpassed signal-to-noise, stability, linearity, and dynamic range.

ED40 Electrochemical Detector

The ED40 is our most powerful and versatile detector, offering three modes of electrochemical detection. It provides the power of suppressed conductivity detection that is offered by the CD20, as well as two forms of electrochemical detection; dc amperometry and integrated amperometry.

Advanced Detection Capabilities

Suppressed conductivity detection with the CD20 can determine tracelevel anions in the presence of higher level anions in the same run.

Determine common cations using the ED40 with SRS Self-RegeneratingCation Suppression, and the EG40 Eluent Generator.

13047-01

The compatibility of the ED40 using integrated amperometry is a powerful tool for the separation of complex mixtures of mono-, di-, oligo-, and polysaccharides.

13026

13740

Amperometry cell

11873

AD25 Absorbance Detector

The AD25 is a dual beam, variable wavelength detector. Itfeatures both a deuterium and tungsten light source to ensureoptimum performance across a broad wavelengh range of 190-800 nm, with extremely low baseline noise, accurateresponse and exceptional wavelengh accuracy. Validation issimplified by using a built in holmium oxide filter for fast, sim-ple verification of wavelengh accuracy.

VARIOUS COMPONENTS DETECTEDBY ION CHROMATOGRAPHY

DETECTION MODE ANALYTES

Conductivity CD20/ED40 • Inorganic anions

• Inorganic cations• Chromate• Disinfection by-products• Ethanolamines• Amines• Ionic surfactants• Organic acids• Polyphosphates• Sulfonates• Azide• Borate• Perchlorate

DCAmperometryED40 • Cyanide

• Sulfide/sulfite• Iodide

Integrated AmperometryED40 • Carbohydrates

• Alcohols/glycols• Amines• Catecholamines• Phenols• Sulfides• Thiols• Sugar alcohols• Amino acids

UV/VIS AD25 • Transition metals

• Nitrate/nitrite• Silicate• Chelating agents• Nonionic surfactants• Lanthanide metals• Stains and dyes• Pesticides/herbicides• Drugs/pharmaceuticals• Nucleic acids• Purines/pyrimidines• Proteins/peptides

The AD25 UV-Vis absorbance detector extends the capability of the DX-500 to include transition metals analysis.

DS3 and standard conductivity cell

7 8

Dionex offers two chromatographic pumps for the DX-500 IC system. Our high performance gradientpump, the GP50, is a sophisticated eluent delivery system designed to pump mixtures of up to four mobile phase components, and either perform isocratically, or deliver linear or exponential gradients.Accurate proportioning and precise eluent deliveryensure consistent results—day in and day out. The IP25 isocratic pump is used exclusively for isocratic eluent delivery.

Superior Flow Control

Both the GP50 and IP25 have a Dual Digital SignalProcessor (DSP) control to provide exceptional flow rateaccuracy for optimal run-to-run reproducibility and areaprecision. DSP ensures superior flow stability over awide range of backpressures.

Precise Eluent Proportioning

The GP50 gradient pump provides precise eluent proportioning regardless of system back pressure or solvent viscosity. Low void volumes result in sharp gradients with minimal delays.

Simple Maintenance and Calibration

Built-in user-service menus with on-screen directionsmake pump calibration simple. The GP50 and IP25pumps are designed with an easy access housing mechanism that slides out for routine maintenance.

High Performance Pumps

The GP50 and IP25 pumps provide exceptional flow control for run-to-run reproducibility (0.1% standard bore, 0.2% microbore).

The GP50 provides low void volumes which result in sharp and precisegradients as demonstrated by these 1% steps.

14285

14283

Rugged PEEK Design

Dionex continues to improve pump design to ensure

durability for challenging laboratory applications.

For long life and minimal maintenance, the GP50

and IP25 pumps are constructed of rugged PEEK

components. The PEEK-based flow paths and

mechanical components of the GP50 and IP25 are

chemically inert. PEEK flow paths guarantee that the

metal contamination commonly found when using

stainless steel pumps is eliminated.

Extend Your IC Capabilities with Additional Options

Vacuum Degas Option

The GP50 and IP25 pumps feature a built-in vacuum

degas option to provide in-line degassing for optimum

reproducibility, low backgrounds, and protection of

solvents from contamination.

Microbore or Standard Bore Configurations

To optimize trace analysis and amino acid

applications, the GP50 and IP25 pumps are offered

in microbore configurations. Benefits of the microbore

configuration include increased sensitivity, lower

detection limits, and reduced eluent consumption.

Eluents like dilute sulfuric acid and sodiumhydroxide cannot corrode pump componentsand tubing due to chemically inert, PEEK-based flow paths.

9 10

The demand for increased efficiency, sample through-

put, and documentation is playing an increasingly

important role in laboratories today. To maximize the

capabilities of the DX-500 IC system, automation tools

such as a PeakNet Chromatography Workstation

or the AutoSelect AS50 Autosampler can be added for

continuous unattended operation. With PeakNet,

the entire chromatography system—from sample

dilution to eluent generation—can be controlled from

one software package.

PeakNet Chromatography W o r k s t a t i o n

PeakNet 6 provides a secure, stable computing environ-

ment to fully address GLP/GMP. Flexible system securi-

ty features allow laboratories to impose appropriate data

control based on their work environment and individual

needs. Whether you wish to work in a completely open

and accessible environment, or operate with restricted

access and controlled privileges, PeakNet 6 gives you the

power to manage your lab’s IC data.

Powerful Automat i o n

The PeakNetSequence Browserallows you to perform batch oper -ations and accessresults.

PeakNet conveniently displays integration views of all injections.

Control panels for each ion chromatograph module (pump, detector,autosampler, etc.) can be established to display instrument conditions.Graphical icons alow intuitive remote operation, including running asequence or schedule of injections.

AutoSelect AS50 Autosampler

The AutoSelect AS50 is a versatile, accurate, random-

vial access autosampler. Designed for reliability and

increased sample throughput, the AS50 delivers high

precision and low carryover to guarantee reproducible

results with injection volumes ranging from 1 µL to

1.5 mL. Metal-free flow paths ensure sample integrity.

Automate sample dilution and reagent addition

with the AS50 Sample Preparation option. The Column

Selection Valve option saves time by automatically

switching between two independent applications

on the same IC system. The AS50 supports two

chromatography enclosure options to house and

protect IC components: the Thermal Compartment

provides improved site-to-site reproducibility for high

sensitivity work, and the Chromatography

Compartment organizes components for routine work

not requiring a temperature controlled environment.

AS40 Automated Sampler

The AS40 Automated Sampler is a rugged,

economical, sample loading device for the DX-500.

This autosampler features a chemically inert, metal-free

flow path. The AS40 design makes operation and

maintenance simple.

AS50 ThermalChromatographyCompartment configured with the Column Switching Option.

Discover How Powerful IC Can Be

Bring the full power of IC to your laboratory.

For complete information on the DX-500, contact

your nearest Dionex representative.

Guaranteed Quality

Purchasing a Dionex product is an investment in

quality. All Dionex products are designed, developed,

and manufactured under an NSAI registered ISO 9001

Quality System and warranted against defects in

materials and workmanship.

The Dionex Commitment to Customer Satisfaction

As a Dionex customer, you have access to worldwide

customer service and support. Our Customer Service

organization has earned a reputation for timely

and professional service, expert training, and skilled

applications assistance.

A Complete Selection of Separation Science Pr o d u c t s

In addition to ion chromatography systems and

modules, Dionex offers a complete selection of products

for your laboratory, including:

• HPLC systems

• Chromatography data systems

• On-line process analyzers

• Accelerated Solvent Extraction systems

• Automation products

• Columns, eluents, suppressors, and accessories

For more information, demonstrations,

and no-obligation quotations, call your

Dionex re p re s e n t a t i v e .

Corporate Headquarters

Dionex Corporation

1228 Titan Way

P.O. Box 3603

Sunnyvale, CA94088-3603

TEL: (408) 737-0700

FAX: (408) 730- 9403

Salt Lake City Technical Center

(Extraction Specialists)

1515 West 2200 South, Suite A

Salt Lake City, UT 84119-1484

TEL: (801) 972-9292

FAX: (801) 972-9291

U.S. Regional Offices

Sunnyvale, CA (408) 737- 8522

Westmont, IL (630) 789- 3660

Atlanta, GA (770) 432- 8100

Houston, TX (281) 847-5 652

Marlton, NJ (856) 596- 0600

International Subsidiaries

Austria (01) 616 51 25

Belgium (015) 203800

Canada (905) 844-9650

France 01 39 46 08 40

Germany 06126 991-0

Italy (06) 66 51 50 52

Japan (06) 6885 -1213

Netherlands (0161) 43 43 03

Switzerland (062) 205 99 66

United Kingdom (01276) 691722

Internet

www.dionex.com

Dionex products are designed, developed and manufactured under an NSAI registeredISO 9001 Quality System.

IonPac, AutoSuppression, AutoRegen, ASRS, SRS and Self-Regenerating Suppressors are registered trademarks and AutoSelect,MicroMembrane, MetPac,and CarboPac are trademarks of Dionex Corporation.Maltrin is a trademark of Grain Processing Corporation.Specifications subject to change without notice.

© 1999 Dionex Corporation LPN 0502-03 10K 11/99 Printed in U.S.A.

I C | H P L C | E X T R A C T I O N | P R O C E S S | A U T O M A T I O N