Dual role of IL-12 in the therapeutic efficacy or failure ... role of IL-12... · T0 T4 T12 T24 0...
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Transcript of Dual role of IL-12 in the therapeutic efficacy or failure ... role of IL-12... · T0 T4 T12 T24 0...
-
0
3
6
0
3
6 ***
IL-6
NI HCV NT
0
12
24
CT HCV - NT
0
12
24
***
IL-10
0
250
500
0
250
500
IL-12
0
10
20
0
10
20
***
TNF-αγ
0
15
30
0
15
30
IFN-
[pg/m
L]
0
50
100
0
50
100 ***
IL-8
[pg
/mL]
NI HCV NT
0
10
20
CT
HCV - NT
0
10
20 **
IL-4
[pg/m
L]
CYTOKINE PROFILE Legend
-
T0 T4 T12 T24
100
102
104
106
108
-
IL-8 IL-6 IL-12TNF-α
IFN-γ
T0 T4 T12 T24
IL-4
T0 T4 T12 T24
IL-10
T0 T4 T12 T24
PAIRED CYTOKINE PROFILES
Time after Treatment
Se
rum
Le
ve
l[p
g/m
L]
T0 T4 T12 T24
PAIRED VIRAL LOAD
HCV - SVR
HCV - NR
100
104
108
Vire
mia
[IU
/mL]
Cut-off – 200 IU/mL
Mean CT = 33
Mean CT = 0.1
Mean CT = 95
Mean CT = 0.1
Mean CT = 3.5
B C ED
F G H
A
0
10
20
0
20
40
0
13
26
0
150
300
0
7
14
0
5
10
0
5
10
Mean CT = 0.6Mean CT = 1.2
-
0
200
400
600
800
0
70
140
0
200
400
600
800
0
70
140
T4/T0
Time Ratio
T12/T0 T24/T0 T4/T0 T12/T0 T24/T0
Ba
selin
e F
old
Change
HCV - SVR HCV - NR
CHANGES IN THE BASELINE CYTOKINE PATTERN
IL-8 IL-6
IL-12
TNF-α
IFN-γ
IL-4
IL-10
IL-8
IL-6
IL-12
TNF-α
IFN-γ
IL-4
IL-10
A B
-
0
30
60 >T0 >T0
* *
0
15
30
T0,>T24
T0 T4 T12 T240
7
14
*
*
*
*
>T0>T0
>T0
0
30
60* *
0
15
30
0.0
1.5
3.0
T0 T4 T12 T240
13
26
**
*
*
T0 T4 T12 T240
7
14*
**
*
Fre
quency o
f G
ate
d C
ells
(%
)
CD69+ Monocytes
Time after Treatment
Time after Treatment
% o
f gate
d lym
phocyte
s
Cell
Ratio
Fre
quency o
f G
ate
d C
ells
(%
)
CD69+ Lymphocytes
CD4+ T-cells CD8+ T-cells CD4+/CD8+ T-cell Ratio
Fre
quency o
f G
ate
d C
ells
(%
)
CD69+ Monocytes
% o
f gate
d lym
phocyte
s
Cell
Ratio
CD69+ Lymphocytes
CD4+ T-cells CD8+ T-cells CD4+/CD8+ T-cell Ratio
Fre
quency o
f G
ate
d C
ells
(%
)HCV-SVR
HCV-N
R
CELL SUBSETS AND ACTIVATION STATUS
A
B
