Droplet Gradient Array For Parallel Experimentation via Microfluidic Device Lindsay Pickett Faculty...
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Transcript of Droplet Gradient Array For Parallel Experimentation via Microfluidic Device Lindsay Pickett Faculty...
Droplet Gradient Array For Parallel Experimentation via Microfluidic Device
Droplet Gradient Array For Parallel Experimentation via Microfluidic Device
Lindsay PickettFaculty Mentor: Abraham P. Lee
Graduate Mentor: Rob LinIM-SURE 2006
Lindsay PickettFaculty Mentor: Abraham P. Lee
Graduate Mentor: Rob LinIM-SURE 2006
Outline of DiscussionOutline of Discussion
IntroductionGoals
ProcessResultsIssues
SolutionsFuture Direction
IntroductionGoals
ProcessResultsIssues
SolutionsFuture Direction
IntroductionMicrofluidic Devices
IntroductionMicrofluidic Devices
• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications
• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication
• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications
• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication
Project GoalProject Goal
Droplet Gradient Array
Ismagilov Laboratory– Thorough characterization– Control over droplet properties
• Droplet volume•Gradient steps
– Versatility of device for multiple applications
Droplet Gradient Array
Ismagilov Laboratory– Thorough characterization– Control over droplet properties
• Droplet volume•Gradient steps
– Versatility of device for multiple applications
Project OutlineProject Outline
Process: Device designFabrication TestingExternal valve
systemComputer-
mediated step gradient
Characterization
Process: Device designFabrication TestingExternal valve
systemComputer-
mediated step gradient
Characterization
QuickTime™ and a decompressor
are needed to see this picture.
Device DesignDevice Design
Outlet reservoir
Reagent Inlets
Oil Inlet
Shearing JunctionArray Observation Region
Process: Device designProcess: Device design
12 µm
7.5 µm
70/30 µm 50/50 µm 12/7.5 µm12/7.5 µm
Process: Experimental SetupProcess: Experimental Setup
Results:Testing
Results:Testing
• Top Aq. Inlet: DI (variable rate)
• Bottom Aq. Inlet: DI + dye (const. rate)
• Oil inlet: Silicon oil (const. rate 2µL/min)
*Droplet content control*
• Top Aq. Inlet: DI (variable rate)
• Bottom Aq. Inlet: DI + dye (const. rate)
• Oil inlet: Silicon oil (const. rate 2µL/min)
*Droplet content control*
0.80 vs 0.70 µL/min
0.95 vs 0.70 µL/min
1.25 vs 0.70 µL/min
QuickTime™ and a decompressor
are needed to see this picture.
Results: Array Capture (Colorcam)
Results: Array Capture (Colorcam)
• Array image capture external valves work• Not pictured instability introduced by valves
– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability
• Array image capture external valves work• Not pictured instability introduced by valves
– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability
Solutions…Solutions…
VALVES• Contacted vendor…
– no suitable suggestions
– different fluid route (Dr. Lee)
• Different External Valves• Quake pressure-
actuation Valves• 2-D valve for sufficient
freeze
VALVES• Contacted vendor…
– no suitable suggestions
– different fluid route (Dr. Lee)
• Different External Valves• Quake pressure-
actuation Valves• 2-D valve for sufficient
freeze
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Other Solutions…Other Solutions…
*Fluid re-routing…*new filtration design… *input tips*Fluid re-routing…*new filtration design… *input tips
Future DirectionFuture Direction
• Valves/new designs• Computer-mediated
step gradient (Hyperterminal)
• Characterization via GFP and ImageJStep gradientDroplet volume
• Valves/new designs• Computer-mediated
step gradient (Hyperterminal)
• Characterization via GFP and ImageJStep gradientDroplet volume
AcknowledgementsAcknowledgements
• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.
• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.