Driving GPC/SEC ForwardPSS supplies certified standards that meet the requirements of DIN 55672 and...

Driving GPC/SEC Forward

Reference Materials & LC Columns

Driving GPC/SEC Forward

PSS GmbH was founded in 1985 by 2 PhD students at the Physical Chemistry Department, University of Mainz, Germany,

producing polymer standards at the University facilities. The following years PSS expanded staff and products includ-

ing tailor-made polymers, organic and aqueous GPC/SEC columns, GPC/SEC software and moved 2001 to own facilities

located in Mainz, Germany. PSS-USA opened its office in Houston, Texas in 1994, moved to Silver Spring, Maryland, in

1995 and since 2003 operates from Warwick, Rhode Island. To date, PSS has successfully gained leadership in the over-

all GPC/SEC market, making innovative contributions not only in Germany and the USA, but around the world.

PSS is fully dedicated to the advancement of macromolecular liquid chromatography, by means of materials design,

synthesis, manufacturing, consulting, service, and innovative research, applying the highest standard of expertise and

reliability. Our close relationship with our costumers has helped us to continuously improve the quality of our products

and services. Our high caliber staff, mostly chemists, is experienced, creative and trained in problem solving. Corpora-

tions, universities, and organizations in more than 60 countries use our products and profit from our outstanding

service and know-how.

Certified DIN ISO EN 9001

PSS is certified (DIN ISO EN 9001:2008) to produce high quality reference polymers, GPC/SEC columns and

software for the characterization of polymers by their molecular weight and their structural character-

istics. PSS employs the latest findings in polymer science for the synthesis and characterization of

polymers, block copolymers and biopolymers. PSS operates manufacturing facility equipped with a

complete state-of-the-art characterization laboratory at the headquarters in Mainz, Germany, fully

supporting customers working under stringent requirements i.e., GLP, DIN, ISO certifications.

About PSS

About this catalog

This catalog contains the most frequently ordered consumables.

Please refer to the website for a comprehensive listing of our products.

Contact us to inquire about custom materials or services.

ContactPSS Polymer Standards Service GmbH

In der Dalheimer Wiese 5

D-55120 Mainz, Germany

Phone: +49 6131 96239-0

Fax: +49 6131 96239-11

[email protected]

www.polymer.de

PSS-Polymer Standards Service – USA Inc

43 Jefferson Blvd. Suite 3

Warwick, RI 02888 USA

Phone: (401) 780 8884

Fax: (401) 780 8824

[email protected]

www.pssgpcshop.com

Contents

1| Molecular Weight Reference Polymers 4

1.1. Polydispersity 4

1.2. Methodology for Characterization Levels 4

1.3. Polymer Part Numbers Deviation 5

1.4. Permanent and Temporary Standards Stock 6

1.5. Specialty Polymers 6

1.6. Polymer Standards Handling 6

1.7. Warranty 7

2| Polymer Standards Temporary Stock and Specialty Polymers 8

2.1. Specialty Polymer 8

3| Polymer Standards Permanent Stock 9

3.1. Organic Standards 9

3.2. Aqueous Standards 12

4| Assembled Kits of Molecular Weight Standards 14

4.1. GPC/SEC Calibration Kits 14

4.2. Organic Standards 14

4.3. Aqueous Standards 16

4.4. ReadyCal Kits 18

4.5. DIN Kits 19

4.6. MALDI Validation Kits 20

4.7. Light Scattering/Viscometry Validation Kit 20

5| Standards for Membrane Characterization 21

6| GPC/SEC System Suitability Test with PSS EasyValid Validation Kit 22

7| Successful GPC/SEC Separation 23

8| GPC/SEC Columns Powerful solutions for size separation 24

8.1. Column Selection 24

8.2. Advantages of PSS Column Technology 26

8.3. Column Order Deliverables 26

8.4. Column Handling 27

8.5. Warranty 28

9| Columns for Organic Solvents 29

9.1. SDV Columns 29

9.2. GRAM Columns 32

9.3. PFG Columns 33

9.4. POLEFIN Columns 34

10| Columns for Aqueous Columns 35

10.1. SUPREMA Columns 35

10.2. NOVEMA Columns 37

10.3. MCX Columns 38

10.4. PROTEEMA Columns 39

11| GPC/SEC Application Guide 40

12| Summary of PSS Columns Specifications 42

4 Reference Materials & LC Columns4

PSS is one of the world‘s largest manufacturers of ref-

erence polymers copolymers, and customer-designed

polymers. A great selection of polymer types in a wide

range of molar masses is produced regularly.

The PSS reference materials are characterized extensively

by modern analytical methods. They are available in vari-

ous characterization levels: GPC, DIN Certified Standards,

MALDI validation standards, Light Scattering, Viscometry

and Certified Reference Materials. Their suitability for a

given application depends on the polydispersity, or the

level of characterization. Reference polymers come as

individual standards or assembled into kits. The kit com-

ponents are carefully selected to cover given molecular

weight ranges to establish calibration curves.

1| Molecular Weight Reference PolymersFor broad standards the Mp value is a function of the col-

umn resolution and therefore not defined.

Broad standards are characterized by Mw and Mn.

Modern GPC/SEC software packages allow the construc-

tion of a calibration curve by the use of Mw, Mn or the

intrinsic viscosity [η] of broad standards.

Up to 8 different broad standards can be used and

only one of the Mn, Mw, or [η] values is nescessary.

Broad standards are used:

• to validate a chromatographic system

• to determine physical constants such as the Mark-

Houwink constants K and a.

• to construct a calibration curve

• to proof a column missmatch.

1.1.1. Methodology for Characterization Levels

PSS uses GPC/SEC to obtain molecular weight values,

which are subsequently confirmed by alternative meth-

ods; e.g., Laser Light Scattering, Viscometry, MALDI-ToF,

VPO and NMR. Each product comes with a Quality

Certificate, which provides the characterization informa-

tion. The signed Quality Certificates include the testing

parameters, the molecular weight values (Mn, Mw, Mp,

PDI) and usually the chromatogram with the parameters

(conditions) at which the data was obtained.

GPC

/SE

C St

anda

rds

PSS Polymer standards in the stock are analyzed via GPC/SEC, yielding Mn, Mw, Mp and the polydispersity (PDI = Mw /Mn ).

DIN

cer

tifi

ed S

tand

ards

PSS supplies certified standards that meet the requirements of DIN 55672 and ISO/EN 13885. Also the PSS Quality Certificates fulfill all DIN and ASTM requirements.

PSS documents the molecular weight using GPC and additonal methods such as Light Scattering, MALDI-Tof, NMR, Viscometry or VPO.

1.2. Methodology for Characterization Levels

PSS uses GPC/SEC to obtain molecular weight values,

which are subsequently confirmed by alternative

methods; e.g., Laser Light Scattering, Viscometry, MALDI-

ToF, VPO and NMR. Each product comes with a Quality

Certificate, which provides the characterization informa-

tion. The signed Quality Certificates include the testing

parameters, the molecular weight values (Mn, Mw, Mp,

PDI) and usually the chromatogram with the parameters

(conditions) at which the data was obtained.

1.1. Polydispersity

In principle the distinction of broad and narrow standards

is based on the PolyDispersityIndex, PDI = Mw/Mn.

Narrow Standards

Narrow polymer standards are made by living ionic

polymerization techniques or by fractionation of very

broad standards. All narrow standards have small poly-

dispersities with sharp and slim chromatogram profile.

The Mp value is well defined and independent of the col-

umn resolution.

TIP: Mp (molar mass at the peak maximum) is the only

well defined point in the chromatogram and so far dedi-

cated in order to construct a calibration curve.

Narrow standards are defined by average molar mass

values such as Mn (Number average molar mass) and Mw

(Weight average molar mass).

Mn and Mw are well established values to define the

molar mass distribution of a polymer.

The construction of the calibration curve in GPC is based

on the Mp values of the polymers in a calibration kit,

e.g., an ensemble of polymers of the same type but dif-

ferent molar masses.

Broad Standards

Broad reference materials are made by radical polymeri-

zation, polycondensation or coordinative polymerization

(polyolefins). The PDI of broad standards is usually >1.5.

5

MA

LDI

Stan

dard

s

PSS MALDI standards are suitable for the validation and comparison of MALDI instruments. PSS provides MALDI spectra including Mw, Mn and Mp values of selected narrow polymers, which differ in molar mass and polymer type (polarity). Furthermore the quality certificate comes with GPC data (Mw, Mn and Mp) and molar mass information of one more absolute method such as NMR, Light Scattering, Viscometry, VPO (depending on polymer type and molar mass).

The Quality Certificate includes the GPC parameters as well as condi-tions and values of MALDI-ToF measurements.

Polymer Standards Service GmbH Polymer Standards Service USA, IncIn der Dalheimer Wiese 5 D-55120 Mainz 43 Jefferson Blvd. Suite 3 Warwick, RI 02888 USATel.: +49 (0) 6131-96239-0 Fax: +49 (0) 6131-96239-11 Phone: +1 401-780-8884 Fax: +1 401-780-8824Email: [email protected] www.polymer.de Email: [email protected] www.pssgpcshop.com

Certificate of Analysis

Manufacture and control according to PSS method of analysis

Dr. T. Hofeproduction director

Storage: Store the tightly recapped polymer standard in a dry, dark, cool area;e.g. a refrigerator (4 °C).Expiration date: 5 years after purchase date provided standard is stored asdescribed above.Purchase date:

Note:Mw = Weight average molecular weightMn = Number average molecular weightMp = Molar mass at the peak maximumPDI= Polydispersity Index

Polymer type: Poly(styrene)Part No: PSS-mps1.5kLot No: ps24040ma

Molar Mass Distribution

0.5

1.0

1.5

2.0

2.5

3.0

3.5

Molar mass [Da]

1*10 2 1*10 3 1*10 4

W(lo

g M

)

PSS

Win

GP

C U

nity

, Bui

ld 6

727,

LA

B_P

RO

D3,

Inst

anz

#1

GPC/SEC - Conditions

Sample concentration 1,00 g/lSolvent THFPrecolumn [8 x 50 mm] PSS SDV 5µmColumns [analytical, each 8 x 300 mm] PSS SDV 5µm 10e3Å / 10e5Å / 10e6ÅData Acquisition Software PSS WinGPC

Inject volume 20 µlFlow rate 1,00 ml/minTemperature 23 °C

Operator S. Fugmann

GPC/SEC - Results

Detector Mw [Da] Mn [Da] Mp [Da] PDI [Mw/Mn]

MALDI-ToF - Results

1 550Mw [Da] Mn [Da]

1 480Calculated Mw, MALDI = Mn MALDI x (Mw/Mn) GPC

Shodex RI 71 1380 1320 1 370 1,05

Ligh

t Sc

atte

ring

/Vi

scom

etry

Sta

ndar

ds

PSS Light Scattering/ Viscometry Standards are suitable for the easy, fast and exact valida-tion and data comparison of Light Scattering and Viscometry instru-ments. PSS provides GPC, Light Scattering and Viscometry data for various narrow and broad distributed polymers.

The Quality Certificate includes the molar mass averages of different methods the GPC parameters as well as conditions of Light Scattering (LS) and/or Viscometry (Visco) measurements.

Custom Request Polymerizations, Specialty Polymers

and Polymer Networks

PSS masters a wide variety of different polymerization

techniques, thus we can synthesize almost all kinds of

homopolymers, functionalized homopolymers, copoly-

mers and polymer networks.

Polymerization bases include but are not limited to:

• Acrylate, Methacrylate

• Styrene

• Diene

• Vinyl

• Isobutylene

• Ethlyleneoxide

Synthesis techniques include but are not limited to:

• Ionic polymerization (anionic, cationic)

• Radical polymerization

• Suspension polymerization

1.3. Polymer Part Numbers Deviation

Polymer Part Numbers reflect the nominal

“Weight Average Molecular Weight” of the polymer.

Unless otherwise specified, the actual molecular weight

of a polymer shipped may vary from the nominal

mostly within a range of ±10% Molecular Weight

deviation of the listed value;

e.g. part number PSS-ps700 will have an actual Mw that

is somewhere within the range of 630 - 770 Da.

Exact values appear in the label of the vial and Quality

Certificate.

1 Characterization of Polymer Reference materials

Classification of Polymer Standards

Characterization MethodsAvailable as

Individual

Standard Kit

GPC organic and aqueous

GPC 4 4

DIN certified GPC + absolute method* 4 4

CRMGPC + Light Scattering

+ Viscosity and physical onstants (round robin test)

4

LS/Visco ValidationGPC + Light Scattering +

Viscosity 4

MALDI ValidationGPC + MALDI + Absolute

method* 4

ReadyCal GPC 4

Specialty polymers: Deuterated Highly tactic, etc.

GPC 4


Certificate of Analysis

Polymer type: Poly(styrene)Part No: PSS-mps1.5kLot No: ps24040ma

MALDI-ToF - Conditions

MALDI-ToF - Plot

Page 2 of 2

Instrument: Hewlett-Packard G2030AShots: 250Matrix: DithranolMonomer repeat units: 104 DaPolymer endgoups: C2H5-(CH)-CH3- / HSide products: none

Comment:Please note, that for some reasons (reslution, detection, matrix, counterion) the weight average molecular weight and the number average molecular weight-values for synthetic polymers, measured by MALDI-ToF, do not represent the true molecular weight distribution of the polymer.

Cert

ifie

d Re

fere

nce

M

ater

ials

CRM

Certified Reference Materials (CRM) are highly characterized polymers certified by German Federal Institute for Material Research and Testing (BAM). Their reported molar mass result from round robin experiments at certified laboratories. The CRM’s quality certificates are full reports with the results of the different characterization methods: GPC, Light Scattering and Viscometry. Further, the certificate includes additional non-certified data, (MALDI-ToF; NMR; DSC and in some instances Rheology data) to provide the most sophisticated set of documentation for a particular polymer available worldwide.


Only one page available; The complete documentation will be delivered with the product.

Certified Reference Material

BAM-P001

Poly(styrene)(PS)

Batch No.: ps2088

CH3

CH3

CH3

C CH2 CH H

n

6 Reference Materials & LC Columns

Samples Preparation for GPC Analysis

1. Prepare fresh standard solutions to assure accurate

concentrations. Precise concentration values are

required when using Light Scattering, Viscometry or

Triple Detection systems.

2. Select the appropriate clean dissolution container

(autosampler vial, bottle, flask, etc.).

3. Add an appropriate quantity of sample using an

analytical balance.

Start with the concentrations provided in table 2 as

guideline.

Select your working concentration based on detection

type and number of columns in use.

The moisture content should be determined before

starting any measurements

Dry the polymer if necessary.

Its variability depends on several parameters

(e.g. surface, molecular weight, sample preparation,

storage conditions, etc.).

4. Adjust the desired sample concentration by an exact

weight. Add the solvent volume accurately.

5. Note: If you use an internal standard for flow rate

monitoring, mix it with a fraction of mobile phase in

a separate container. Use the marked solvent to pre-

pare your standards. Do not add it into the general

solvent bottle.

TIP: The internal standard should be monodisperse, and

elute after the separation limit.

6

1.4. Permanent and Temporary Standards Stock

PSS offers permanent and temporary stock of reference

materials. The stock of permanent materials is renewed

when a particular lot sells out whereas the temporary

part numbers will be only avalable while supplies last.

Production of temporary part numbers will be discontin-

ued.

Temporary Standards are listed in chapter 2, Permanent

Standards are listed in chapter 3 (starting from page 9)

and in chapter 4 (Kits starting from page 13).

Look up the Temporary Standards comprehensive list

at www.polymer.de. The listing is updated regularly.

1.5. Specialty Polymers

Specialty Polymers are polymers, which are not poly-

merized as “classical reference materials” regarding to

molar mass variation. The term “Specialty Polymers”

includes all different kinds of copolymers and functional-

ized or deuterated polymers. They are also offered on a

temporary basis. Please visit our website

www.polymer.de for an updated list.

TIP: PSS will tailor Specialty Polymers exhibiting new

topologies or novel properties, in close co-operation with

our customers. Call us for a consultation and a personal

quotation.

Copolymers

PSS offers a multitude of different copolymers. The offer

contains various topologies, compositions, architectures

as well as deuterated and partly deuterated products.

For an extensive list of our actually available copolymers

please visit our website www.polymer.de. The list is

constantly extended by new products.

1.6. Polymer Standards Handling

Storage of Polymer Standards

Store the standards in a dry, dark, cool area; e.g. a

refrigerator (4oC), and tightly recapped. This keeps

excessive moisture away and increases the standard

shelf life.

WARNING: Do not store standards exposed to direct sun-

light (windowsill or shelf near windows), to prevent

sample degradation over time. This condition is critical to

Poly(isoprene), Poly(butadiene) and other temperature

and/or light sensitive polymers.

TIP: Use only freshly prepared solutions and use them up

within two days. Solutions may be stored in the refriger-

ator temporarily.

WARNING:

Do not freeze standards in solution; freezing can destroy

the sample.

7

6. Close or seal the dissolution container and let sit at

room temperature for 24 hours or overnight for com-

plete dissolution.

TIP: Let polymers of Mw < 200 000 Da, sit for 3-4 hours.

Ultra High Molecular weight standards and samples

>2 000 000 Da may take from 1-3 days to dissolve

completely.

7. Gently swirl the vials for a homogeneous solution.

WARNING: Do not use stir bars, ultrasonic baths, micro-

wave heating or harsh shaking as this can cause sample

degradation.

8. Standard Mixes: It is possible to dissolve up to four

standards of the same type in a given dissolution

container. If you make your mixture, the individual

standards molecular weight must be separated by

orders of magnitude to prevent coelution; e.g.

Poly(styrene): 5 000 Da, 50 000 Da, 500 000 Da.

PSS offers pre-made mixtures or kits that prevent

coelution (see ReadyCal-Kits). Note: separation will

depend on the column(s) you use.

TIP: When using a viscometer or Light Scattering detec-

tor, your standard mixture peaks must reach the baseline

between each standard injected in order for the standard

to be used in a calibration curve.

9. Inject each solution separately.

Start with the guideline below (Table 3) to develop

your optimum injection volume.

10. Ultra High Molecular Weight Samples

(Mw > 1 000 000 Da) elute best in low concentra-

tions, through columns of large particle (10µm,

20µm) and pore sizes.

Work at reduced flow rates (0.5 – 0.3 ml/min) and if

necessary increased injection volume. This reduces

the probability of sample degradation. Example:

3 Recommended Injection VolumeNumber of columns

(length 30 cm)Guideline injection volume

[µl]

4 - 5 and more 200 to 250

3 100

2 50

1 20

2 Recommended Sample ConcentrationMolecular Weight Range [Da] Concentration [g/l] or [mg/ml]

100 - 10 000 1 to 2

10 000 - 100 000 1 to 2

100 000 - 500 000 1 to 2

500 000 - 1 000 000 1 to 2

1 000 000 and above 0,5 or less

broad standards usally 4.0 - 5.0 mg/ml

Inject 100 µl of a solution of 0.1 g/l instead of 20 µl

of a solution of 0.5 g/l.

11. Use the measured Mp values of the standards to

construct a calibration curve.

1.7. Warranty

PSS Polymer Standards

Service offers the warranty for the standards according to

specifications given in the Quality Certificate under the

following circumstances:

1. Polymers shall be stored in a cool, dry and dark

environment.

2. Do not expose the standards to UV radiation.

3. Prevent undue stress including, but not limited to,

shaking, high-pressure filtration, ultrasound, and or

microwaves, during sample dissolution and/or actual

testing.

PSS will replace any sealed standard, which was stored

according to conditions mentioned above, within one

month of its purchase date. Consumable materials are

excluded from return and exchange when seal is broken.


2| Polymer Standards Temporary Stock and Specialty Polymers

2.1. Specialty Polymer

10 CopolymersExcerpt of the available copolymers:

Poly(styrene-b-methyl methycrylate)

Poly(styrene-b-n-butyl methacrylate)

Poly(styrene-b-vinylpyridine)

Poly(styrene-b-butadiene)

Poly(styrene-b-isoprene)

Poly(styrene-b-dimethyl siloxane)

Poly(styrene-b-alpha-methylstyrene)

Poly(styrene-b-acrylic acid)

Poly(styrene-b-ethylene oxide)

Poly(methyl methacrylate-b-n-butyl methacrylate)

Poly(methyl methacrylate-b-t-butyl methacrylate)

Poly(butadien-b-methacrylic acid)

Poly(isoprene-b-butadiene)

6 Deuterated PolymersPolymer Molar mass range [Da]

min. max.

Poly(butadiene-1.4-d6) 23 000 190 000

Poly(carbonate-d4) broad 50 000

Poly(dimethyl siloxane-d6) 5 000 25 000

Poly(methyl-methacrylate-d8) 4 000 580 000

Poly(para-methylstyrene-d10) 100 000

Poly(styrene-d3) 2 500 160 000

Poly(styrene-d8) 900 830 000

Poly(styrene sulfonate-d8) lithium salt 80 000 90 000

7 Polymers with Functional End groupPolymer Molar mass range [Da]

min. max.

Poly(butadiene-1.4) OH-end group 10 000

Poly(ethylene glycol) dimethylether 90 2 000

Poly(styrene) brominated 40 000 230 000

Poly(styrene) fluorescein labeled 600 5 000 000

Poly(styrene) sulfonated labeled 5 000 18 000

Poly(styrene) with deuterium end group 2 000 70 000

Poly(styrene) without initiator end group 2 000 60 000


9 Star PolymersPolymer Molar mass range [Da]

min. max.

Poly(isoprene-1,4) 3-star 30 000 150 000

Poly(styrene) 3-star 45 000 300 000

Poly(styrene) 8-star 120 000 230 000

4 Temporary Stock of Organic StandardsPolymer Molar mass range [Da]

min. max.

Nylon 6 broad 15 000 40 000

Nylon 6.6 broad 32 000 110 000

Poly(carbonate) broad 4 000 60 000

Poly(ethylene) broad 50 000 100 000

Poly(isoprene-3.4) 1 000 75 000

Poly(isopropyl methacrylate) 14 000 100 000

Poly(n-propyl methacrylate) 15 000 60 000

Poly(ortho-chlorostyrene) 100 000 300 000

Poly(para-methylstyrene) 3 000 800 000

Poly(propylene) 700 3 500

Poly(propylene) broad 6 000 350 000

Poly(vinyl acetate) broad 17 000 300 000

Poly(vinyl chloride) 42 000 300 000

Poly(vinyl pyrrolidone) broad 14 000 1 000 000

5 Temporary Stock of Aqueous StandardsPolymer Molar mass range [Da]

min. max.

Hydroxypropyl cellulose 12 000 60 000

Hydroxypropyl cellulose broad 30 000 900 000

Poly(2-vinylpyridinium bromide) 3 000 3 300 000

Poly(acrylamide) broad 3 500 9 000 000

Poly(DADMAC) 45 000 450 000

Poly(styrene sulfonate) sodium salt broad 100 000 500 000

Poly(styrene) without initiator end group 2 000 60 000


8 Highly Tactic PolymersPolymer Molar mass range [Da]

min. max.

Poly(methyl methacrylate) isotactic 5 000 60 000

Poly(methyl methacrylate) syndiotactic 8 000 400 000

9

3| Polymer Standards Permanent Stock

3.1. Organic Standards

11 Oligo(ethylene) monodispersePart Number Molar Mass [Da]* PDI Weight [mg]

PSS-oe72 72 1.00 1000

PSS-oe114 114 1.00 1000

PSS-oe200 200 1.00 1000

PSS-oe254 254 1.00 1000

PSS-oe340 340 1.00 1000

PSS-oe450 450 1.00 1000

PSS-oe500 500 1.00 1000

This catalog represents items available on a regular

basis and therefore, deliverable immediately.

12 Poly(2-vinylpyridine)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pvp1k 1 000 <1.50 1000

PSS-pvp2.1k 2 100 <1.50 1000

PSS-pvp4.7k 4 700 <1.15 1000

PSS-pvp10k 10 000 <1.15 1000

PSS-pvp21k 21 000 <1.15 1000

PSS-pvp45k 45 000 <1.15 1000

PSS-pvp110k 110 000 <1.15 1000

PSS-pvp265k 265 000 <1.15 1000

13 Poly(alpha-methylstyrene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-ams1.5k 1 500 <1.50 1000

PSS-ams4k 4 000 <1.15 1000

PSS-ams8k 8 000 <1.15 1000

PSS-ams16k 16 000 <1.15 1000

PSS-ams29k 29 000 <1.15 1000

PSS-ams60k 60 000 <1.15 1000

PSS-ams110k 110 000 <1.15 1000

PSS-ams230k 230 000 <1.15 1000

PSS-ams430k 430 000 <1.15 1000

PSS-ams850k 850 000 <1.15 1000

14 Poly(butadiene-1.2) Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-bdt1k 1 000 <1.50 1000

PSS-bdt1.8k 1 800 <1.50 1000

PSS-bdt3.2k 3 200 <1.15 1000

PSS-bdt5.6k 5 600 <1.15 1000

PSS-bdt12k 12 000 <1.15 1000

PSS-bdt20k 20 000 <1.15 1000

15 Poly(butadiene-1.4)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-bdf2.1k 2 100 <1.15 1000

PSS-bdf4.7k 4 700 <1.15 1000

PSS-bdf10k 10 000 <1.15 1000

PSS-oe500 500 1.00 1000

PSS-bdf21k 21 000 <1.15 1000

PSS-bdf47k 47 000 <1.15 1000

PSS-bdf100k 100 000 <1.15 1000

PSS-bdf210k 210 000 <1.15 1000

PSS-bdf470k 470 000 <1.15 1000

PSS-bdf1m 1 000 000 <1.50 1000

16 Poly(dimethyl siloxane)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pdm162 162 1.00 500

PSS-pdm237 237 1.00 500

PSS-pdm311 311 1.00 500

PSS-pdm1.5k 1 500 <1.50 500

PSS-pdm3.5k 3 500 <1.25 500

PSS-pdm10k 10 000 <1.15 500

PSS-pdm30k 30 000 <1.15 500

PSS-pdm100k 100 000 <1.15 500

17 Poly(ethylene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pe1.2k 1 200 <2.00 250

PSS-pe2k 2 000 <2.00 250

PSS-pe17k 17 000 <2.00 250

PSS-pe21k 21 000 <2.00 250

PSS-pe37k 37 000 <2.00 250

PSS-pe60k 60 000 <2.00 250

PSS-pe77k 77 000 <2.00 250

PSS-pe85k 85 000 <2.00 250

PSS-pe92k 92 000 <2.00 250

PSS-pe110k 110 000 <2.00 250

PSS-pe150k 150 000 <2.00 250

PSS-pe170k 170 000 <2.00 250

PSS-pe180k 180 000 <2.00 250

18 Poly(ethylene terephthalate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pet3.5k 3 500 <2.10 250

PSS-pet10k 10 000 <2.10 250

PSS-pet18k 18 000 <2.10 250

PSS-pet25k 25 000 <2.10 250

PSS-pet35k 35 000 <2.10 250

PSS-pet40k 40 000 <2.10 250

PSS-pet50k 50 000 <2.10 250

PSS-pet75k 75 000 <2.10 250

PSS-pet120k 120 000 <2.10 250

15 Poly(butadiene-1.4)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-bdf110 110 1.00 1000

PSS-bdf470 470 <1.50 1000

PSS-bdf1k 1 000 <1.50 1000


19 Poly(ethyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-em1.8k 1 800 <1.50 1000

PSS-em5k 5 000 <1.15 1000

PSS-em8k 8 000 <1.15 1000

PSS-em45k 45 000 <1.15 1000

PSS-em145k 145 000 <1.15 1000

PSS-em165k 165 000 <1.15 1000

PSS-em280k 280 000 <1.15 1000

PSS-em300k 300 000 <1.15 1000

PSS-em350k 350 000 <1.15 1000

PSS-em500k 500 000 <1.15 1000

20 Poly(isobutylene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pib112 112 1.00 250

PSS-pib390 390 <1.30 250

PSS-pib1k 1 000 <1.30 250

PSS-pib2.1k 2 100 <1.30 250

PSS-pib4.7k 4 700 <1.30 250

PSS-pib10k 10 000 <1.30 250

PSS-pib21k 21 000 <1.30 250

PSS-pib47k 47 000 <1.30 250

PSS-pib100k 100 000 <1.30 250

PSS-pib210k 210 000 <1.30 250

PSS-pib470k 470 000 <1.30 250

PSS-pib700k 700 000 <1.30 250

21 Poly(isoprene-1.4)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pio800 800 <1.50 1000

PSS-pio1k 1 000 <1.50 1000

PSS-pio2.1k 2 100 <1.15 1000

PSS-pio4.7k 4 700 <1.15 1000

PSS-pio10k 10 000 <1.15 1000

PSS-pio21k 21 000 <1.15 1000

PSS-pio47k 47 000 <1.15 1000

PSS-pio100k 100 000 <1.15 1000

PSS-pio210k 210 000 <1.15 1000

PSS-pio470k 470 000 <1.15 1000

PSS-pio1m 1 000 000 <1.50 1000

22 Poly(methyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-mm470k 470 000 <1.15 1000

PSS-mm1m 1 000 000 <1.50 1000

PSS-mm2m 2 000 000 <1.50 1000

PSS-mm3m 3 000 000 <1.50 1000

22 Poly(methyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-mm102 102 1.00 1000

PSS-mm202 202 1.00 1000

PSS-mm600 600 <1.50 1000

PSS-mm1k 1 000 <1.50 1000

PSS-mm2.1k 2 100 <1.15 1000

PSS-mm4.7k 4 700 <1.15 1000

PSS-mm10k 10 000 <1.15 1000

PSS-mm21k 21 000 <1.15 1000

PSS-mm47k 47 000 <1.15 1000

PSS-mm100k 100 000 <1.15 1000

PSS-mm210k 210 000 <1.15 1000

23 Poly(methyl methacrylate) broad)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-mmb20k 20 000 >1.50 1000

PSS-mmb60k 60 000 >1.50 1000

PSS-mmb100k 100 000 >1.50 1000

PSS-mmb2.2m 2 200 000 >1.50 1000

24 DIN-Poly(methyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-dmm450 450 <1.30 1000

PSS-dmm3.5k 3 500 <1.10 1000

PSS-dmm5k 5 000 <1.10 1000

PSS-dmm14k 14 000 <1.05 1000

PSS-dmm23k 23 000 <1.05 1000

PSS-dmm45k 45 000 <1.05 1000

PSS-dmm65k 65 000 <1.05 1000

PSS-dmm90k 90 000 <1.05 1000

PSS-dmm170k 170 000 <1.05 1000

PSS-dmm350k 350 000 <1.05 1000

PSS-dmm600k 600 000 <1.05 1000

PSS-dmm850k 850 000 <1.10 1000

25 CRM-Poly(methyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-cmm350k 365 900 1.25 1000

26 CRM-Poly(methyl methacrylate)broadPart Number Molar Mass [Da]* PDI Weight [mg]

PSS-cmmb100k 101 100 2.15 1000

PSS-cmmb350k 366 400 2.23 1000

27 CRM-Poly(lactide)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-cpla90k 92 400 1.68 1000

PSS-cpla230k 249 400 1.98 1000

28 Poly(n-butyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-nb1k 1 000 <1.50 1000

PSS-nb2.8k 2 800 <1.15 1000

PSS-nb5.5k 5 500 <1.15 1000

PSS-nb12k 12 000 <1.15 1000

PSS-nb20k 20 000 <1.15 1000

PSS-nb47k 47 000 <1.15 1000

PSS-nb100k 100 000 <1.15 1000

PSS-nb210k 210 000 <1.15 1000

PSS-nb470k 470 000 <1.15 1000

PSS-nb700k 700 000 <1.15 1000

11

29 Poly(propylene glycol)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-ppg76 76 1.00 1000

PSS-ppg192 192 1.00 1000

PSS-ppg450 450 <1.25 1000

PSS-ppg790 790 <1.25 1000

PSS-ppg1k 1 000 <1.25 1000

PSS-ppg3k 3 000 <1.25 1000

PSS-ppg5k 5 000 <1.25 1000

30 Poly(styrene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-ps162 162 1.00 1000

PSS-ps266 266 1.00 1000

PSS-ps370 370 1.00 1000

PSS-ps560 560 <1.50 1000

PSS-ps1k 1 000 <1.50 1000

PSS-ps1.8k 1 800 <1.50 1000

PSS-ps3.2k 3 200 <1.15 1000

PSS-ps5.6k 5 600 <1.15 1000

PSS-ps10k 10 000 <1.15 1000

PSS-ps18k 18 000 <1.15 1000

PSS-ps33k 33 000 <1.15 1000

PSS-ps56k 56 000 <1.15 1000

PSS-ps100k 100 000 <1.15 1000

PSS-ps180k 180 000 <1.15 1000

PSS-ps320k 320 000 <1.15 1000

PSS-ps560k 560 000 <1.15 1000

PSS-ps1m 1 000 000 <1.50 1000

PSS-ps1.8 1 800 000 <1.50 1000

PSS-ps3.2m 3 200 000 <1.50 1000

PSS-ps5m 5 000 000 <1.50 1000

PSS-ps10m 10 000 000 <1.50 1000

PSS-ps20m 20 000 000 <1.50 1000

31 Poly(styrene) broad Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-psb45k 45 000 >1.50 1000

PSS-psb100k 100 000 >1.50 1000

PSS-psb250k 250 000 >1.50 1000

PSS-psb450k 450 000 >1.50 1000

33 CRM-Poly(styrene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-cps80k 79 600 1.08 1000

34 CRM-Poly(styrene) broadPart Number Molar Mass [Da]* PDI Weight [mg]

PSS-cpsb180k 181 200 2.26 1000

PSS-cpsb330k 311 800 2.25 1000

35 Poly(t-butyl acrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-tba27k 27 000 <1.25 1000

PSS-tba47k 47 000 <1.25 1000

PSS-tba100k 100 000 <1.25 1000

PSS-tba210k 210 000 <1.25 1000

PSS-tba470k 470 000 <1.25 1000

PSS-tba1m 1 000 000 <1.50 1000

36 Poly(t-butyl methacrylate)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-tbma1.5k 1 500 <1.50 1000

PSS-tbma2.1k 2 100 <1.15 1000

PSS-tbma4.7k 4 700 <1.15 1000

PSS-tbma10k 10 000 <1.15 1000

PSS-tbma21k 21 000 <1.15 1000

PSS-tbma47k 47 000 <1.15 1000

PSS-tbma100k 100 000 <1.15 1000

PSS-tbma210k 210 000 <1.15 1000

PSS-tbma470k 470 000 <1.15 1000

PSS-tbma1m 1 000 000 <1.50 1000

32 DIN-Poly(styrene)Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-dps162 162 1.00 1000

PSS-dps700 700 <1.20 1000

PSS-dps1.4k 1 400 <1.20 1000

PSS-dps3.2k 3 200 <1.05 1000

PSS-dps9k 9 000 <1.05 1000

PSS-dps18k 18 000 <1.05 1000

PSS-dps32k 32 000 <1.05 1000

PSS-dps100k 100 000 <1.05 1000

PSS-dps250k 280 000 <1.05 1000

PSS-dps560k 560 000 <1.05 1000

PSS-dps800k 800 000 <1.05 1000

PSS-dps1.8m 1 800 000 <1.20 1000

*) For most part numbers with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for some excep-tions). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in the label of the vial and Quality Certificate.


3.2. Aqueous Standards

38 DextranPart Number Molar Mass [Da]* PDI Weight [mg]

PSS-dxt180 180 1.00 1000

PSS-dxt342 342 1.00 1000

PSS-dxt504 504 1.00 1000

PSS-dxt1.3k 1 300 <1.50 1000

PSS-dxt5k 5 000 <2.00 1000

PSS-dxt12k 12 000 <1.50 1000

PSS-dxt25k 25 000 <1.50 1000

PSS-dxt50k 50 000 <1.50 1000

PSS-dxt80k 80 000 <1.50 1000

PSS-dxt150k 150 000 <1.50 1000

PSS-dxt270k 270 000 <2.00 1000

PSS-dxt410k 410 000 <2.00 1000

PSS-dxt670k 670 000 <2.50 1000

39 Dextran broad/branchedPart Number Molar Mass [Da]* PDI Weight [mg]

PSS-dxtb4k 4 000 >1.50 1000

PSS-dxtb10k 10 000 >1.50 1000

PSS-dxtb40k 40 000 >1.50 1000

PSS-dxtb70k 70 000 >1.50 1000

PSS-dxtb500k 500 000** >1.70 1000

PSS-dxtb1.5m 1 500 000** >1.70 1000

PSS-dxtb3m 3 000 000** >1.70 1000

PSS-dxtb17m 17 000 000** >1.70 1000

40 DIN-Dextran Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-ddxt180 180 1.00 1000

PSS-ddxt342 342 1.00 1000

PSS-ddxt1.3k 1 300 <1.50 1000

PSS-ddxt5k 5 000 <2.00 1000

PSS-ddxt12k 12 000 <1.50 1000

37 Hydroxyethyl starchPart Number Molar Mass [Da]* PDI Weight [mg]

PSS-hes24k 24 000 <2.50 250

PSS-hes50k 50 000 <2.50 250

PSS-hes140k 140 000 <2.50 250

PSS-hes350k 350 000 <2.50 250

PSS-hes800k 800 000 <2.50 250

PSS-hes1.4m 1 400 000 <2.50 250

PSS-hes2m 2 000 000 <2.50 250

41 Poly(acrylic acid) sodium salt Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-paa2k 2 000 <2.00 250

PSS-paa4k 4 000 <2.00 250

PSS-paa8k 8 000 <2.00 250

PSS-paa18k 18 000 <2.00 250

PSS-paa35k 35 000 <2.00 250

PSS-paa130k 130 000 <2.00 250

PSS-paa245k 245 000 <2.00 250

PSS-paa585k 585 000 <2.00 250

PSS-paa1m 1 000 000 <2.00 250

PSS-paa1.3m 1 300 000 <2.00 250

42 Poly(ethylene glycol) Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-peg106 106 1.00 1000

PSS-peg194 194 1.00 1000

PSS-peg232 232 1.00 1000

PSS-peg330 330 <1.25 1000

PSS-peg400 400 <1.25 1000

PSS-peg600 600 <1.25 1000

PSS-peg1k 1 000 <1.25 1000

PSS-peg1.5k 1 500 <1.25 1000

PSS-peg2k 2 000 <1.25 1000

PSS-peg3k 3 000 <1.25 1000

PSS-peg4k 4 000 <1.25 1000

PSS-peg6k 6 000 <1.25 1000

PSS-peg10k 10 000 <1.25 1000

PSS-peg12k 12 000 <1.25 1000

PSS-peg18k 18 000 <1.25 1000

PSS-peg26k 26 000 <1.25 1000

PSS-peg42k 42 000 <1.30 1000

40 DIN-Dextran Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-ddxt25k 25 000 <1.50 1000

PSS-ddxt50k 50 000 <1.50 1000

PSS-ddxt80k 80 000 <1.50 1000

PSS-ddxt150k 150 000 <1.50 1000

PSS-ddxt270k 270 000 <2.00 1000

PSS-ddxt410k 410 000 <2.00 1000

PSS-ddxt3m 3 000 000 >1.70 1000

13

*) For most part numbers with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for some excep-tions). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in the label of the vial and Quality Certificate.

**) Mw based on light scattering

43 DIN-Poly(ethylene glycol) Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-dpeg106 106 1.00 1000

PSS-dpeg194 194 1.00 1000

PSS-dpeg400 400 <1.25 1000

PSS-dpeg1k 1 000 <1.25 1000

PSS-dpeg2k 2 000 <1.25 1000

PSS-dpeg3k 3 000 <1.25 1000

PSS-dpeg6k 6 000 <1.25 1000

PSS-dpeg12k 12 000 <1.25 1000

PSS-dpeg26k 26 000 <1.25 1000

PSS-dpeg42k 42 000 <1.30 1000

44 Poly(ethylene oxide) Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-peo42k 42 000 <1.30 1000

PSS-peo110k 110 000 <1.35 1000

PSS-peo220k 220 000 <1.35 1000

PSS-peo500k 500 000 <1.50 1000

PSS-peo1m 1 000 000 <1.35 1000

45 CRM-Poly(ethylene oxide) Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-cpeo6k 6 200 1.06 1000

PSS-cpeo11k 11 350 1.11 1000

46 Poly(methacrylic acid) sodium salt Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pma1.2k 1 200 <1.20 500

PSS-pma3.5k 3 500 <1.20 500

PSS-pma7.6k 7 600 <1.20 500

PSS-pma18k 18 000 <1.20 500

PSS-pma36k 36 000 <1.20 500

PSS-pma76k 76 000 <1.20 500

PSS-pma160k 160 000 <1.20 500

PSS-pma340k 340 000 <1.20 500

PSS-pma500k 500 000 <1.20 500

47 Poly(styrene sulfonate) sodium salt Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pss1k 1 000 <1.20 500

PSS-pss3.4k 3 400 <1.20 500

PSS-pss6k 6 000 <1.20 500

PSS-pss15k 15 000 <1.20 500

PSS-pss30k 30 000 <1.20 500

PSS-pss67k 67 000 <1.20 500

PSS-pss140k 140 000 <1.20 500

PSS-pss280k 280 000 <1.20 500

PSS-pss600k 600 000 <1.20 500

PSS-pss1m 1 000 000 <1.20 500

PSS-pss2m 2 000 000 <1.20 500

48 Poly(vinyl alcohol) broad Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-pvo5k 5 000 <2.50 1000

PSS-pvo12k 12 000 <3.50 1000

PSS-pvo30k 30 000 <2.50 1000

PSS-pvo40k 40 000 <2.50 1000

PSS-pvo75k 75 000 <2.50 1000

PSS-pvo100k 100 000 <2.50 1000

PSS-pvo120k 120 000 <2.50 1000

PSS-pvo160k 160 000 <2.50 1000

49 DIN-Pullulan Part Number Molar Mass [Da]* PDI Weight [mg]

PSS-dpul342 342 1.00 100

PSS-dpul1.3k 1 300 <1.30 100

PSS-dpul6k 6 000 <1.20 100

PSS-dpul12k 12 000 <1.20 100

PSS-dpul22k 22 000 <1.20 100

PSS-dpul50k 50 000 <1.20 100

PSS-dpul110k 110 000 <1.20 100

PSS-dpul200k 200 000 <1.20 100

PSS-dpul400k 400 000 <1.20 100

PSS-dpul800k 800 000 <1.20 100

PSS-dpul1.6m 1600 000 <1.20 100

PSS-dpul2.5m 2 500 000 <1.50 100

Reference Materials & LC Columns14

4| Assembled Kits of Molecular Weight Standards

4.2. Organic Standards

50 Kit Oligo(ethylene) monodisperse

PSS-oekit 8 x 500 mgMw Part [Da]* PDI

72 1.00

86 1.00

114 1.00

170 1.00

225 1.00

280 1.00

390 1.00

500 1.00

51 Kit Poly(2-vinylpyridine)

PSS-pvpkit 8 x 1000 mg Mw Part [Da]* PDI

1 000 < 1.50

2 100 < 1.50

4 700 < 1.15

10 000 < 1.15

21 000 < 1.15

45 000 < 1.15

110 000 < 1.15

265 000 < 1.15

52 Kit Poly(alpha-methylstyrene)

PSS-amskit 10 x 1000 mg Mw Part [Da]* PDI

60 000 < 1.15

110 000 < 1.15

230 000 < 1.15

430 000 < 1.15

850 000 < 1.15

53 Kit Poly(butadiene-1.4)

PSS-bdfkit 10 x 1000 mg Mw Part [Da]* PDI

1 000 < 1.50

2 100 < 1.50

4 700 < 1.15

10 000 < 1.15

18 000 < 1.15

35 000 < 1.15

100 000 < 1.15

170 000 < 1.15

500 000 < 1.15

1 000 000 < 1.50

52 Kit Poly(alpha-methylstyrene)

PSS-amskit 10 x 1000 mg Mw Part [Da]* PDI

1 500 < 1.50

4 000 < 1.15

8 000 < 1.15

16 000 < 1.15

29 000 < 1.15

4.1. GPC/SEC Calibration Kits

A calibration kit consists of 8 to 12 well-characterized

standards of one polymer type. The kits include a calibra-

tion report using the individual standards, as well as the

analytical conditions to create the calibration curve.

A Quality Certificate accompanies each standard with all

pertinent analytical parameters and molar mass distribu-

tion via GPC.

The composition of a kit may change.

Please visit www.polymer.de for an updated list.

15

56 Kit Poly(isobutylene)

PSS-pibkit 10 x 250 mg Mw Part [Da]* PDI

350 < 1.30

1 000 < 1.30

2 100 < 1.30

4 700 < 1.30

10 000 < 1.30

29 000 < 1.30

47 000 < 1.30

150 000 < 1.30

300 000 < 1.30

700 000 < 1.30

57 Kit Poly(isoprene-1.4)

PSS-piokit 10 x 1000 mg Mw Part [Da]* PDI

1 000 < 1.50

2 100 < 1.15

4 700 < 1.15

10 000 < 1.15

21 000 < 1.15

47 000 < 1.15

100 000 < 1.15

210 000 < 1.15

470 000 < 1.15

1 000 000 < 1.15

58 Kit Poly(L-lactide) low

PSS-plakit 8 x 200 mg Mw Part [Da]* PDI

150 1.00

700 < 1.15

1 500 < 1.15

2 500 < 1.15

10 000 < 1.15

20 000 < 1.15

28 000 < 1.20

40 000 < 1,40

59 Kit Poly(L-lactide) high

PSS-plakith 8 x 200 mg + 1 (broad) x 500 mg

Mw Part [Da]* PDI

150 1.00

700 < 1.15

1 500 < 1.15

2 500 < 1.15

10 000 < 1.15

20 000 < 1.15

28 000 < 1.20

40 000 < 1,40

200 000 > 1.50

60 Kit Poly(methyl methacrylate) high

PSS-mmkith 12 x 500 mg Mw Part [Da]* PDI

102 1.00

800 < 1.50

2 500 < 1.15

6 500 < 1.15

12 500 < 1.15

23 000 < 1.15

40 000 < 1.15

85 000 < 1.15

210 000 < 1.15

380 000 < 1.15

615 000 < 1.15

1 000 000 < 1.50

54 Kit Poly(dimethyl siloxane)

PSS-pdmkit 8 x 500 mg Mw Part [Da]* PDI

311 1.00

2 000 < 1.50

6 000 < 1.25

15 000 < 1.15

30 000 < 1.15

60 000 < 1.15

150 000 < 1.15

200 000 < 1.15

55 Kit Poly(ethylene)

PSS-pekit 10 x 250 mg Mw Part [Da]* PDI

340 1.00

500 1.00

1 200 < 2.00

2 000 < 2.00

17 000 < 2.00

37 000 < 2.00

60 000 < 2.00

85 000 < 2.00

110 000 < 2.00

180 000 < 2.00

61 Kit Poly(methyl methacrylate) low

PSS-mmkitl 8 x 500 mg Mw Part [Da]* PDI

102 1.00

800 < 1.50

2 100 < 1.15

3 000 < 1.15

4 700 < 1.15

6 500 < 1.15

8 000 < 1.15

17 000 < 1.15


64 Kit Poly(styrene) high

PSS-pskith 12 x 1000 mg Mw Part [Da]* PDI

560 < 1.50

1 200 < 1.50

3 200 < 1.15

8 000 < 1.15

18 000 < 1.15

33 000 < 1.15

62 000 < 1.15

120 000 < 1.15

280 000 < 1.15

560 000 < 1.15

1 200 000 < 1.15

2 500 000 < 1.50

66 Kit Poly(t-butyl methacrylate)

PSS-tbmakit 10 x 1000 mg Mw Part [Da]* PDI

1 500 < 1.50

2 100 < 1.15

4 700 < 1.15

10 000 < 1.15

16 000 < 1.15

40 000 < 1.15

100 000 < 1.15

300 000 < 1.15

600 000 < 1.15

1 000 000 < 1.50

65 Kit Poly(styrene) low

PSS-pskitl 8 x 1000 mg Mw Part [Da]* PDI

162 1.00

560 < 1.50

1 200 < 1.50

2 500 < 1.15

3 200 < 1.15

4 500 < 1.15

8 000 < 1.15

18 000 < 1.15

4.3. Aqueous Standards

67 Kit Dextran

PSS-dxtkit 10 x 500 mg Mw Part [Da]* PDI

180 1.00

342 1.00

1 300 < 2.00

5 000 < 2.00

12 000 < 1.50

25 000 < 1.50

50 000 < 1.50

150 000 < 1.50

270 000 < 2.00

410 000 < 2.00

68 Kit Hydroxyethyl starch

PSS-heskit 7 x 250 mg Mw Part [Da]* PDI

24 000 < 2.50

50 000 < 2.50

140 000 < 2.50

350 000 < 2.50

800 000 < 2.50

1 400 000 < 2.50

2 000 000 < 2.50

62 Kit Poly(n-butyl methacrylate)

PSS-nbkit 9 x 1000 mg Mw Part [Da]* PDI

1 500 < 1.50

3 500 < 1.15

7 500 < 1.15

20 000 < 1.15

47 000 < 1.15

100 000 < 1.15

210 000 < 1.15

470 000 < 1.15

700 000 < 1.15

63 Kit Poly(propylene glycol)

PSS-ppgkit 7 x 1000 mg Mw Part [Da]* PDI

76 1.00

192 1.00

450 < 1.25

790 < 1.25

1 000 < 1.25

3 000 < 1.25

5 000 < 1.25

17

70 Kit Poly(ethylene glycol)

PSS-pegkit 10 x 500 mg Mw Part [Da]* PDI

106 1.00

194 1.00

400 < 1.25

1 000 < 1.25

2 000 < 1.25

3 000 < 1.25

6 000 < 1.25

12 000 < 1.25

26 000 < 1.25

42 000 < 1.30

71 Kit Poly(ethylene oxide)

PSS-peokit 8 x 500 mg Mw Part [Da]* PDI

25 000 < 1.35

42 000 < 1.35

60 000 < 1.35

110 000 < 1.35

180 000 < 1.35

270 000 < 1.35

500 000 < 1.50

1 000 000 < 1.50

72 Kit Poly(methacrylic acid) sodium salt

PSS-pmakit 8 x 500 mg Mw Part [Da]* PDI

1 200 < 1.20

3 500 < 1.20

7 600 < 1.20

36 000 < 1.20

76 000 < 1.20

160 000 < 1.20

340 000 < 1.20

500 000 < 1.20

73 Kit Poly(styrene sulfonate) sodium salt

PSS-psskit 10 x 500 mg Mw Part [Da]* PDI

1 000 < 1.20

3 400 < 1.20

6 000 < 1.20

15 000 < 1.20

30 000 < 1.20

67 000 < 1.20

140 000 < 1.20

280 000 < 1.20

600 000 < 1.20

1 000 000 < 1.20

74 Kit Pullulan

PSS-pulkit 10 x 100 mg Mw Part [Da]* PDI

342 1.00

1 300 < 1.30

6 000 < 1.20

10 000 < 1.20

22 000 < 1.20

50 000 < 1.20

110 000 < 1.20

200 000 < 1.20

400 000 < 1.20

800 000 < 1.30

*) For all parts with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for broad standards). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in the label of the vial and Quality Certificate.

69 Kit Poly(acrylic acid) sodium salt

PSS-paakit 10 x 250 mg Mw Part [Da]* PDI

2 000 < 2.00

4 000 < 2.00

8 000 < 2.00

18 000 < 2.00

35 000 < 2.00

130 000 < 2.00

245 000 < 2.00

585 000 < 2.00

1 000 000 < 2.00

1 300 000 < 2.00


4.4. ReadyCal Kits

75 ReadyCal-Kit Poly(methyl methacrylate)

PSS-mmkitr4 3 x 10 Vials-4.0mlPSS-mmkitr1 3 x 10 Vials-1.5ml

Mw Part [Da]* PDI

800 < 1.50

2 500 < 1.15

6 500 < 1.15

12 500 < 1.15

23 000 < 1.15

40 000 < 1.15

85 000 < 1.15

210 000 < 1.15

380 000 < 1.15

615 000 < 1.15

1 000 000 < 1.15

1 500 000 < 1.50

PSS ReadyCal Standards are polymer cocktails pre

weighted into auto-sampler vials. Each vial has three or

four polymers of the same type with different molar

masses. Carefully selected molar masses are placed into

each vial so that the polymer traces are baseline separat-

ed (if a proper column combination is used)

A ReadyCal Kit allows you to prepare fast and repro-

ducible a 8 - 12 point calibration curve without the mess

of weighing samples. Just add solvent directly into the

auto sampler vial, let it stand for two hours, shake

gently and load into your auto sampler for analysis.

Each kit contains 30 autosampler vials (for at least 10

calibration curves) that are color coded for your

convenience.

Available in 1.5ml and 4.0ml vials.

76 ReadyCal-Kit Poly(styrene) high

PSS-pskitr4h 3 x 10 Vials-4.0mlPSS-pskitr1h 3 x 10 Vials-1.5ml

Mw Part [Da]* PDI

1 500 < 1.50

3 200 < 1.15

8 000 < 1.15

18 000 < 1.15

33 000 < 1.15

62 000 < 1.15

120 000 < 1.15

238 000 < 1.15

560 000 < 1.15

1 200 000 < 1.50

2 500 000 < 1.50

6 500 000 < 1.50

77 ReadyCal-Kit Poly(styrene) low

PSS-pskitr4l 3 x 10 Vials-4.0mlPSS-pskitr1l 3 x 10 Vials-1.5ml

Mw Part [Da]* PDI

266 1.00

560 < 1.50

1 200 < 1.50

2 500 < 1.50

3 200 < 1.15

4 500 < 1.15

8 000 < 1.15

18 000 < 1.15

33 000 < 1.15

62 000 < 1.15

78 ReadyCal-Kit Poly(styrene)

PSS-pskitr4 3 x 10 Vials-4.0mlPSS-pskitr1 3 x 10 Vials-1.5ml

Mw Part [Da]* PDI

560 < 1.50

1 200 < 1.50

3 200 < 1.15

8 000 < 1.15

18 000 < 1.15

33 000 < 1.15

62 000 < 1.15

120 000 < 1.15

280 000 < 1.15

560 000 < 1.15

1 200 000 < 1.50

2 500 000 < 1.50

19

79 ReadyCal-Kit PEO/PEG

PSS-peokitr4 3 x 10 Vials-4.0mlPSS-peokitr1 3 x 10 Vials-1.5ml

Mw Part [Da]* PDI

232 1.00

600 < 1.25

2 000 < 1.25

6 000 < 1.25

18 000 < 1.25

42 000 < 1.30

110 000 < 1.35

270 000 < 1.35

500 000 < 1.50

1 000 000 < 1.50

80 ReadyCal-Kit Poly(ethylene glycol)

PSS-pegkitr1 3 x 10 Vials-1.5mlMw Part [Da]* PDI

232 1.00

330 < 1.25

600 < 1.25

1 000 < 1.25

1 500 < 1.25

3 000 < 1.25

4 000 < 1.25

6 000 < 1.25

12 000 < 1.25

18 000 < 1.25

26 000 < 1.25

42 000 < 1.30

4.5. DIN Kits

81 DIN-Kit Poly(methyl methacrylate)

PSS-mmkitd 12 x 500 mg Mw Part [Da]* PDI

450 < 1.30

3 500 < 1.10

5 000 < 1.10

14 000 < 1.05

23 000 < 1.05

45 000 < 1.05

65 000 < 1.05

90 000 < 1.05

170 000 < 1.05

350 000 < 1.05

600 000 < 1.05

850 000 < 1.10

82 DIN-Kit Poly(styrene)

PSS-pskitd 12 x 1000 mg Mw Part [Da]* PDI

162 1.00

700 < 1.20

1 400 < 1.20

3 200 < 1.05

9 000 < 1.05

18 000 < 1.20

32 000 < 1.05

100 000 < 1.05

250 000 < 1.05

560 000 < 1.05

800 000 < 1.05

1 800 000 < 1.20

83 DIN-Kit Dextran

PSS-dxtkitd 10 x 500 mg Mw Part [Da]* PDI

180 1.00

342 1.00

1 300 < 1.50

5 000 < 2.00

12 000 < 1.50

25 000 < 1.50

50 000 < 1.50

150 000 < 1.50

270 000 < 2.00

410 000 < 2.00

84 DIN-Kit Poly(ethylene glycol)

PSS-pegkitd 10 x 500 mgMw Part [Da]* PDI

106 1.00

194 1.00

400 < 1.25

1 000 < 1.25

2 000 < 1.25

3 000 < 1.25

6 000 < 1.25

12 000 < 1.25

26 000 < 1.25

42 000 < 1.30


4.6. MALDI Validation Kits

PSS provides Polymer Standard Kits that will help you to

check, calibrate and validate a Matrix-Assisted-Laser-

Desorption-Ionization-Time-of-Flight measurement (MALDI-

ToF) instrument. Standards with different molecular

weight ranges and different polarities are included. The

different molecular weights allow you to determine the

resolution of the instrument as a function of the molecular

weight, whereas different polymer polarities help you

determine the compatibility of your Matrix and polymer.

4.7. Light Scattering/ViscometryValidation Kit

The kit is useful to check the instrument calibration and

the delay volume between the concentration detector

and the molar mass detector.

This kit includes a mixture of well defined Light

Scattering (LS) and/or Viscometry (Visco) reference

materials (narrow and broad distributed) with the

relevant Light Scattering and/or Viscometry data. The

validation of your Light Scattering or Viscometry instru-

ment is simple, fast and reliable.

85 MALDI-Kit Poly(methyl methacrylate)

PSS-mmkitm 6 x 500 mgMw Part [Da]* PDI

450 < 1.50

2 500 < 1.15

5 000 < 1.15

10 000 < 1.15

23 000 < 1.15

60 000 < 1.15

*) For all parts with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for broad standards). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in the label of the vial and Quality Certificate.

86 MALDI-Kit Poly(styrene)

PSS-pskitm 6 x 500 mgMw Part [Da]* PDI

700 < 1.50

1 200 < 1.50

5 000 < 1.15

9 000 < 1.15

34 000 < 1.15

65 000 < 1.15

87 MALDI-Kit Poly(ethylene glycol)

PSS-pegkitm 6 x 500 mgMw Part [Da]* PDI

400 < 1.25

1 000 < 1.25

3 000 < 1.25

6 000 < 1.25

12 000 < 1.25

26 000 < 1.25

88 MALDI mixed-Kit PS, PMMA, PDMS, PEG, PSS

PSS-mixkitm 5 x 500 mgMw Part [Da]* PDI

Poly(dimethyl siloxane) 4 500 < 1.25

Poly(ethylene glycol) 6 000 < 1.25

Poly(methyl methacrylate) 5 000 < 1.15

Poly(styrene) 5 000 < 1.15

Poly(styrene sulfonate) sodium salt 5 000 < 1.20

89 LS-Visco-Kit Poly(methyl methacrylate)

PSS-mmkitv 4 x 500 mgMw Part [Da]* PDI

8 000 < 1.15

100 000 > 1.50

160 000 < 1.05

850 000 < 1.15

91 LS-Visco-Kit Dextran

PSS-dxtkitv 4 x 500 mgMw Part [Da]* PDI

12 000 < 1.50

50 000 < 1.50

70 000 > 1.50

410 000 < 2.00

90 LS-Visco-Kit Poly(styrene)

PSS-pskitv 4 x 500 mgMw Part [Da]* PDI

9 000 < 1.05

120 000 < 1.05

250 000 > 2.50

560 000 < 1.05

21

GPC/SEC is a fast, robust and significant method for the

characterization of membranes, especially in their native

environment (swelled state). PSS offers the ability to

perform an automated, fast and simple membrane

characterization including cut-off and pore size distribu-

tion determination.

The GPC/SEC membrane characterization is accomplished

by filtering the sample through the membrane.

Depending on the membrane type and quality some

smaller molecules can pass the pores of the membrane

while others will be retained. The filtered and the

un filtered stock solution are then measured on a GPC/

SEC system. The average pore size distribution and the

cut-off the membrane is determined automatically by

comparing the elution profiles of the unfiltered sample

to the filtered fraction.

5| Standards for Membrane Characterization

Mem

bran

e St

anda

rds

Following membrane standards are available:

• Linear Polysaccharide (Pullulan):

Part Nummber: PSS-mepulb300k (Mw: 300 000 Da)

• Branched Polysaccharide (Dextran):

P/N PSS-medxtb70k (Mw: 70 000 Da)

P/N PSS-medxtb2m (Mw: 2 000 000 Da)

• Polystyrene:

P/N PSS-mepsb200k (Mw: 200 000 Da)

Packing sizes: 50 g,100 g, 250 g, 500 g.

These new PSS membrane standards are featured by:

• a broad molar mass distribution

• the molar mass average Mw and Mn

• the integral molar mass information Mmin, Mmax

• the corresponding radii of gyration Rgmin

and Rgmax.


PSS has developed a dedicated GPC/SEC system

suitability test that evaluates the integral system:

equipment, electronics, and analytical operations, using

the PSS EasyValid Validation Kit. This ensures that the

system will yield validated GPC/SEC results.

The PSS EasyValid Validation Kit is designed for the

validation of GPC/SEC instrumentation with concentration

detectors independently of brand.

It consists of:

• a validation column

• calibration standards

• certified reference materials

• WinGPC Unity report layouts

• WinGPC Unity import files

• a comprehensive user documentation.

6| GPC/SEC System Suitability Test with PSS EasyValid Validation Kit

Valid

atio

n Re

port

The Validation Kit EasyValid is ideal:

• for checking the system performance

after installation

• as part of the OQ/PV (Operational

Qualification/Performance Verification)

• for performance review after maintenance

• for inter laboratory consistence checks

• for identifying systematic errors

• for training new employees

For additional validation of molar mass sensitive

detectors use Light Scattering / Viscometry Valdiation

Kits (see page 20)

EasyValid for organic systems

P/N PSS-pskitval

EasyValid for aqueous systems

P/N PSS-dxtkitval

23

Gel Permeation Chromatography (GPC),

Size Exclusion Chromatography (SEC),

or Gel Filtration Chromatography (GFC)

are names used interchangeably for a liquid chromatog-

raphy technique to obtain separation according to mole-

cular dimensions.

Contrary to LAC- (Liquid Adsorption Chromatography)

methods like HPLC, that rely on interactions between

sample and stationary phase (column material),

GPC/SEC must operate free of interactions.

Only then is a separation according the size of the mole-

cules ensured. Ideally, only entropic effects should influ-

ence this type of separation.

The following information should help you to successfully

install GPC/SEC in your lab.

1. Select an appropriate stationary phase (column

material) that fits your samples and ensures interac-

tion free separation.

2. Make sure to select and use a mobile phase (eluent)

that is compatible with the stationary phase (column

material, gel).

3. To improve the resolution and/or expand the molec-

ular weight separation range, PSS recommends to

use multiple columns in series.

7| Successful GPC/SEC Separation

4. Select the proper particle size and the appropriate

pore size distribution.

5. Maintain a flow rate and a temperature that agrees

with the column diameter and viscosity of the sol-

vent to prevent sample shearing and high backpres-

sure.

6. Use precolumns to increase the overall column life

time.

7. Filter your samples through a 0.45 µm filter for 5 µm

particle size or 1 µm filter for 10/20 µm particle size

to prevent solids from entering the column.

8. Determine the plate count, asymmetry and resolu-

tion of your column(s) regularly to check their per-

formance.

9. Determine the molecular weight range of the sepa-

ration with a calibration curve.

10. Use appropriate concentration and injection volume

balance to prevent column overload.

11. The higher the molecular weight of your sample the

lower the sample concentration. Refer to chapter

“Standards Handling” for guidelines.


Fig. 2: PSS Magic Triangle for GPC/SEC applications

PMMA

PS

THF

Toluene

DMF

HFIP

GRAM PFG SUPREMA

WATER

Dextran

PAA

SDV

Sam

ple

Pola

rity Solvent Polarity

Sorbent Polarity

GPC/SEC

Fig. 3: PSS Magic Triangle for aqueous GPC/SEC applications

Neutral Polymers

Polycations

NOVEMA SUPREMA

12

11

10

9

7

8

23

45

6Weak

Polyanions

Strong Polyanions

MCX

Poly

mer

Ioni

c Ch

arac

ter

pH Value

Stationary Phase Polarity

Aqueous GPC/SEC

Our high performance columns are the result of compre-

hensive research focusing on the development of more

efficient and robust gel materials and an improved pack-

ing technology. We manufacture stationary phase gels for

the chromatography able to separate synthetic and natu-

ral macromolecules by their hydrodynamic volume. PSS

offers the widest range of column materials optimized

for aqueous or organic solvents alike:

TIP: See summary of specifications on last page

8.1. Column Selection

A GPC/SEC column can be selected by different criteria

depending on your analytical goal (highest resolution,

product screening, etc.). This chapter will present the

most important and essential criteria.

8| GPC/SEC Columns Powerful solutions for size separation

SDV

SDV Lux

GRAM

PFG

POLEFIN

ORGANIC SOLVENTS

SuPREMA

SuPREMA Lux

NOVEMA

MCX

PROTEEMA

AquEOuS SOLVENTS

Fig. 1: Overview of gel materials

1. Step: Optimum Stationary Phase

The PSS column concept is to minimize interactions

between polymer and stationary phases. In general, the

sample is the determinant factor: the polarity of the

sample defines the polarity of the solvent and therefore

also that of the stationary phase. To ensure interaction

free chromatography the stationary phase needs to be

selected carefully.

PSS allows the selection of stationary phases of different

polarities. The PSS Magic Triangle provides a quick visual

aid for the selection of columns, where the three sides

represent sample, mobile phase, and stationary phase

(see Fig. 2).

25

Handling of the PSS Magic Triangle

Construct an isosceles triangle by locating the polarity of

sample and mobile phase; the peak of the Magic

Triangle guides you to the proper stationary phase.

This concept is also applicable for aqueous GPC/SEC. The

magic triangle for aqueous systems takes the pH value

instead of the stationary phase into account (see Fig. 3).

TIP: Solvent Compatibility

It is imperative that a sample passing through a column

be soluble in the mobile phase. When using a solvent

different from the mobile phase to dissolve and inject a

sample, both solvents must be completely miscible and

compatible with the stationary phase. PSS recommends

to use the mobile phase also for sample preparation.

2. Step: Column Dimensions and Field of Application

Our stationary phases are packed in stainless steel (V4A)

columns of standard dimensions that fit any HPLC or

GPC/SEC instrument

** I.D.: internal diameter ** Any other column dimension on request

TIP:

For additional column dimensions please call us or your

closest representative.

101 Column dimension and field of application

Column I.D.* x length Field of

Type [mm]** application

Precolumn 8 x 50

4.6 x 30 20 x 50

Protection

Analytical8 x 300 8 x 600

Conventional analysis

Micro 4.6 x 250Solvent saving, small

sample amounts

Preparative 20 x 300 20 x 600 40 x 250

Preparative sample fractionation, sample

collection

HighSpeed 20 x 50 Ultra fast analysis

Lux 8 x 300 GPC Light Scattering

Hig

hSpe

ed C

olum

ns

Fig. 4: A HighSpeed column (red line) reduces the run time

for a sample (broad Poly(styrene)) by a factor of 6 - 8 compared to

a conventional column (black line).

Lux

Colu

mns

Fig. 5: Lux columns reach the steady state mode > 25 times faster

than conventional columns, and their overall noise reduction is more

than 25 times better.

Elution Volume [ml]

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Det

ecto

r Si

gnal

[V]


102 Application of different column typesSelect Recommended application

HighSpeed columns Very fast results needed in process

control and high throughput screening

Analysis time: 2 - 4 min per sample

Linear or mixed

bed columns

Product screening at constant peak

resolution Analysis time: ~ 12 min

per sample

Combination of single

porosity columns

Highest resolution and maximum

information Analysis time: > 12 min

per sample

8.3. Column Order Deliverables

Installation Tips

1. Purge the system with mobile phase first. Pump sol-

vent through the system to remove air and to prevent

that air is entering the colums. Dont’ forget to flush

the injection loop.

2. Use the column connections supplied from PSS to

connect columns in series.

PSS carefully tests every column according resolution, asymmetry and plate count number. The quality certificate contains the testing results and the corresponding test chro-matograms.

qua

lity

Cert

ific

ate


Quality Certificate

Column: PSS SUPREMA analytical 100ÅSerial No: 9120753Dimension: 8 x 300 mmParticle size: 5 µmShipped in: Water, Sodium azide 0.05%

0.00

0.02

0.04

0.06

0.08

0.10

0.12

0.14

0.16

Elution Volume [-]

6 7 8 9 10 11 12 13 14 15

50%, dV=0.19 ml

10%

Det

ecto

r Res

pons

e [-]

PSS

Win

GPC

Uni

ty, B

uild

921

5, L

AB_P

RO

D5,

Inst

anz

#1

GPC Column test according to:DIN 55672, ISO 13885

Testsample EGTheoretical plates / m 50 000Asymmetry 1,0

Polymer mixturePullulan mix low

GPC/SEC - Conditions

Solvent Water, Sodium azide 0.05%Flow rate 1,00 ml/minTemperature 23 °CColumn pressure 20 BarDetector RI-DetectorInject volume 20 µlOperator M. NaumannData acquisition software PSS WinGPC

Dr. G. Reinholdproduction director

Manufacture and control according to PSS method of analysis

0.000

0.005

0.010

0.015

0.020

0.025

0.030

Elution Volume [-]

6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5

Det

ecto

r Res

pons

e [-]

PS

S W

inG

PC

Uni

ty, B

uild

921

5, L

AB_

PR

OD

5, In

stan

z #1

Component 1: Mp = 112000 [Da]Component 2: Mp = 11800 [Da]Component 3: Mp = 991 [Da]Component 4: Mp = 180 [Da]

8.2. Advantages of PSS ColumnTechnology

• Excellent separation efficiency obtained with narrowly

classified gels and optimized frit technology.

• Excellent stability under a wide range of physical,

chemical and biological environments.

• High solvent compatibility with organic or aqueous

mobile phases alike.

• Column production under DIN EN ISO 9001 quality

standards.

• Controlled Quality: each column is tested according

to DIN 55672 and ISO/EN 13885

• Delivery with a detailed user documentation and

a quality certificate.

• PSS engineered fittings and their integrated diffusers

provide excellent sample injection.

• Easy to service: easy to change end frits.

• Refill Service: PSS will refurbish cost saving old

columns with new gel-material.

• Dedicated support in questions, problems and

applicational demands from a team of commited

and experienced scientists.

3. Step: Particle Sizes and Porosities

PSS GPC/SEC column materials are available in several

particle sizes from 3 µm up to 20 µm particles.

The optimum particle size depends on

• the solvent viscosity (higher viscosity requires

higher particle size)

• the sample molar mass (higher molar masses

require higher particle sizes).

Porosities

The pore size distribution of the column material

determines the separation range.

• To separate low molecular weight products use

small porosities.

• The higher the molecular weight the higher is the

required porosity.

• Single porosity columns show a high resolution in

a narrow molecular weight range.

• A wide molecular weight range at constant high

resolution is covered if columns with single

porosities are coupled in series.

• Linear or mixed bed columns provide a very wide

porosity range therefore offering a broad separation

range with a smaller resolution.

For any desired application PSS offers the optimum col-

umn or column combination (single porosity or linear).

4. Step: Laboratory workflow requirements/Tasks

Once you know the stationary phase, particle size and

porosity, there are different options to optimize the poly-

mer separation, depending on laboratory workflow

requirements:

TIP: The combination of single porosity columns has the

inherent risk of column mismatch. Use the column com-

binations recommended by PSS to avoid column mis-

match.

27

Fig. 6: Maximum distance between ferrule and capillary end.

8.4. Column Handling

Installation Tips

1. Purge the system with mobile phase first. Pump sol-

vent through the system to remove air and to prevent

that air is entering the colums. Dont’ forget to flush

the injection loop.

2. Use the column connections supplied from PSS to

connect columns in series.

WARNING: Make sure that the fitting tubing protrudes a

maximum of 1 mm inside the column head (see Fig. 6)

Solvent of Choice

PSS delivers the columns in the eluent of the customer

choice, except solvents with a high freezing point like

DMSO and TCB.

The solvent within the columns is marked with colour-

coded screw stops.

Column Connector

PSS ships connection fittings with the column.

Test mixture

PSS ships a polymer mixture for testing with the column

if possible.

User documentation

Each column is delivered with detailed user documen-

tation.

Refill Services

PSS will refill your old columns at a considerable cost

saving upon request. They will be tested individually and

delivered with a new quality certificate.

103 Recommended flow ratesFlow I.D. 8 mm I.D. 4.6 mm I.D. 20 mm

Operating 1 ml/min 0.33 ml/min 6.25 ml/min

Reduced 0.25 ml/min 0.1 ml/min 1.5 ml/min

Idle 0.1 ml/min 0.03 ml/min 0.6 ml/min

3. Be aware of column flow direction. Operating the flow

rate in the reverse direction is only part of trouble-shoot-

ing or operating after a long storage time.

4. Thread the column fittings finger tight into the system.

Do not over tighten the fitting. Overtightening may

damage the column and the column head.

5. Flush the column with 10 times of the column

volume at 1/5 of the recommended flow rate.

6. Eluent should come out after 2-3 minutes at 0.2 ml/

min flow rate. If not, see “Recovering partially dry col-

umns”.

7. When the solvent exchange is completed, connect to

the detector(s).

8. Slowly increase the flow rate to the typical operating

flow rate which depends on the column dimensions

(see table 103).

9. Check the column pressure.

WARNING: Maximum pressure should never exceed twice

the pressure specified in the column certificate.

10. Give the columns time to equilibrate.

11. Check the plate count of the whole chromato-graphic

system incl. columns (see column user documenta-

tion). If this test fails, first check each column sepa-

rately when using a column combination, then con-

tact PSS or a PSS representative.

TIP: For Multiple Columns use

• Install the middle porosity first and use the largest

porosity connected into the detector.

• Use only column combinations recommended by PSS.

• Do not combine linear and single porosity columns.

• Do not combine different particle sizes.

Cleaning

When columns loose efficiency (Rsp, Asymmetry) or you

suspect the presence of foreign material adsorbed on the

column, PSS recommends the following procedures:

• Remove the column from the detector.

• Install the column in reverse direction for clean-up.

• Flush the column at 0.1 ml/min flow rate with a sol-

vent that dissolves the suspect impurities and is fully

compatible with your system, until the impurities are

removed.


TIP: To clean aqueous columns, use variation of pH, buff-

er concentration, or mixtures of organic and aqueous

eluents. Organic columns are cleaned best by variation of

eluent polarity. Use mixtures of appropriate solvents (e.g.

THF in toluene resp. chloroform) or solvent additives

(TFAc in THF).

Storage

PSS recommends to store the columns in the solvent

used during shipment; f.e. columns shipped in DMAc or

DMF must be stored in DMAc or DMF.

1. Remove salt solutions with pure solvent, disconnect

the column from the GPC/SEC system and tightly plug

with the original end plugs.

2. It is a good practice to store columns with volatile

mobile phases in a refrigerator (4°C) to prevent solvent

evaporation.

WARNING: Never let the column temperature fall below

the freezing point of the storage solvent. This will

destroy the stationary phase.

Recovering partially dry columns

Occasionally solvent is lost during long-term storage or

due to high storage temperatures. When after (re-)

installing the column no solvent overflows or the expect-

ed system pressure does not build up, this can be an

indication for a partially dry column.

To re-wet a partially dry column:

• Install the column in the reverse direction.

• Fill the column with solvent, using a flow rate of

0.1 ml/min, until no more bubbles appear at the

column outlet.

• Change the column to the correct flow direction

and use 0.1 ml/min for 2h.

• Increase the flow rate slowly to the operating flow

rate with an increment of 0.2 ml/min per 5 minutes.

8.5. Warranty

PSS Polymer Standards Service offers the warranty for

the columns accorduing to specifications given in the

Quality Certificate.

29

9.1. SDV Columns

104 GPC/SEC of Polymers in unpolar and Medium Polar Organic Solvents

Field of Application

ApplicabilityPoly(styrene), Poly(vinyl chloride), Poly(carbonate), Elastomers, Resins and others

Eluents THF, Toluene, TCM, DCM

Specifications

MaterialStyrene-divinylbenzene copolymer network

Maximum Pressure45 - 150 bar (650 - 2180 psi), depending on porosity

Maximum Temperature 100° C

Maximum Flow Rate3 ml/min (8 mm I.D., 5 resp. 10 μm)

Particle Size 3, 5, 10, 20 μm

Molecular Weight Range 100 to 30 000 000 Da

Incompatibility Water, alcohol, drying, and freezing

9| Columns for Organic Solvents

Separation range50 Å 100 - 5 000 Da

100 Å 100 - 10 000 Da

500 Å 100 - 30 000 Da

1 000 Å 100 - 60 000 Da

10 000 Å 500 - 700 000 Da

100 000 Å 1 000 - 1 000 000 Da

1 000 000 Å 1 000 - 3 000 000 Da

10 000 000 Å 5 000 - 30 000 000 Da

linear S 100 - 150 000 Da

linear M 100 - 1 000 000 Da

linear XL 100 - 3 000 000 Da

Applications

Olig

omer

ic P

oly(

styr

ene)

Flow rate: 0.5 ml/min, Loading: 1 g/l, 20 µl;

Eluent: THF; Temp.: 25° C; Detector: SECcurity RI;

Columns: SDV 5 µm, 50 Å, 100 Å, 100 Å (8 x 300 mm) + precolumn

Gly

ceri

de a

cc. E

uP

Poly

(dim

ethy

l silo

xane

)

Flow rate: 1 ml/min; Loading: 40 g/l, 40 µl;

Eluent: THF; Temp.: 25° C; Detector: SECcurity RI;

Column: SDV 5 µm, 100 Å (8x 600 mm)


Eluent: Toluene; Temp.: 25° C; Detector: SECcurity RI;

Columns: SDV 5 µm, 1000 Å,100 000 Å, 1 000 000 Å (8 x 300 mm) + precolumn

Elution Volume [ml]

Elution Volume [ml]

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Det

ecto

r Si

gnal

[V]

Det

ecto

r Si

gnal

[V]

Recommended Column CombinationLow molar mass: 100 Å + 1 000 Å + 10 000 Å

Med. mol. mass: 1 000 Å + 100 000 Å + 1 000 000 Å

High molar mass: 1 000 Å + 100 000 Å + 10 000 000 Å


Calibration Curves

Part numbers

105 PSS SDV Columns analyticalParticle size

[µm]Porosity

Dimension [mm]

Part Number

3 precolumn 8 x 50 sda080503




3 50 Å 8 x 300 sda0830035e1

3 100 Å 8 x 300 sda0830031e2

3 500 Å 8 x 300 sda0830035e2

3 1 000 Å 8 x 300 sda0830031e3

3 10 000 Å 8 x 300 sda0830031e4

3 100 000 Å 8 x 300 sda0830031e5

5 50 Å 8 x 300 sda0830055e1

5 100 Å 8 x 300 sda0830051e2

5 500 Å 8 x 300 sda0830055e2

5 1 000 Å 8 x 300 sda0830051e3

5 10 000 Å 8 x 300 sda0830051e4

5 100 000 Å 8 x 300 sda0830051e5

5 1 000 000 Å 8 x 300 sda0830051e6

10 50 Å 8 x 300 sda0830105e1

10 100 Å 8 x 300 sda0830101e2

10 500 Å 8 x 300 sda0830105e2

10 1 000 Å 8 x 300 sda0830101e3

10 10 000 Å 8 x 300 sda0830101e4

10 100 000 Å 8 x 300 sda0830101e5

10 1 000 000 Å 8 x 300 sda0830101e6

10 10 000 000 Å 8 x 300 sda0830101e7

20 100 Å 8 x 300 sda0830201e2

20 500 Å 8 x 300 sda0830205e2

20 1 000 Å 8 x 300 sda0830201e3

20 10 000 Å 8 x 300 sda0830201e4

20 100 000 Å 8 x 300 sda0830201e5

20 1 000 000 Å 8 x 300 sda0830201e6

20 10 000 000 Å 8 x 300 sda0830201e7

106 PSS SDV Columns analytical, linearParticle size

[µm]Porosity

Dimension [mm]

Part Number





3 linear S 8 x 300 sda083003lis

3 linear M 8 x 300 sda083003lim



5 linear XL 8 x 300 sda083005lxl






107 PSS SDV Lux Columns analytical (for light scattering)

Particle size [µm]

PorosityDimension

[mm] Part Number

5 precolumn 8 x 50 sdl080505

10 precolumn 8 x 50 sdl080510

5 1 000 Å 8 x 300 sdl0830051e3

5 100 000 Å 8 x 300 sdl0830051e5

5 1 000 000 Å 8 x 300 sdl0830051e6

5 linear M 8 x 300 sdl083005lim

5 linear XL 8 x 300 sdl083005lxl

10 1 000 Å 8 x 300 sdl0830101e3

10 100 000 Å 8 x 300 sdl0830101e5

10 1 000 000 Å 8 x 300 sdl0830101e6

10 linear M 8 x 300 sdl083010lim

10 linear XL 8 x 300 sdl083010lxl

Line

ar C

olum

ns

Sing

le P

oros

ity

Colu

mns

Elue

nt: T

HF;

Cal

ibra

nts:

Po

ly(s

tyre

ne)

Elue

nt: T

HF;

Cal

ibra

nts:

Po

ly(s

tyre

ne)

Elution Volume [ml] Elution Volume [ml]

Mol

ar M

asse

[D

a]

Mol

ar M

asse

[D

a]

31

108 PSS SDV Columns HighSpeedParticle size

[µm]Porosity

Dimension [mm]

Part Number

3 100 Å 20 x 50 sds2005031e2

3 500 Å 20 x 50 sds2005035e2

3 1 000 Å 20 x 50 sds2005031e3

3 10 000 Å 20 x 50 sds2005031e4

3 100 000 Å 20 x 50 sds2005031e5

5 100 Å 20 x 50 sds2005051e2

5 500 Å 20 x 50 sds2005055e2

5 1 000 Å 20 x 50 sds2005051e3

5 10 000 Å 20 x 50 sds2005051e4

5 100 000 Å 20 x 50 sds2005051e5

5 1 000 000 Å 20 x 50 sds2005051e6

10 100 Å 20 x 50 sds2005101e2

10 500 Å 20 x 50 sds2005105e2

10 1 000 Å 20 x 50 sds2005101e3

10 10 000 Å 20 x 50 sds2005101e4

10 100 000 Å 20 x 50 sds2005101e5

10 1 000 000 Å 20 x 50 sds2005101e6

10 10 000 000 Å 20 x 50 sds2005101e7

20 100 Å 20 x 50 sds2005201e2

20 1 000 Å 20 x 50 sds2005201e3

20 10 000 Å 20 x 50 sds2005201e4

20 100 000 Å 20 x 50 sds2005201e5

20 1 000 000 Å 20 x 50 sds2005201e6

20 10 000 000 Å 20 x 50 sds2005201e7

109 PSS SDV Columns HighSpeed, linearParticle size

[µm]Porosity

Dimension [mm]

Part Number

3 linear S 20 x 50 sds200503lis

3 linear M 20 x 50 sds200503lim



5 linear XL 20 x 50 sds200505lxl






111 PSS SDV Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 100 Å 20 x 300 sdp2030101e2

10 500 Å 20 x 300 sdp2030105e2

10 1 000 Å 20 x 300 sdp2030101e3

10 10 000 Å 20 x 300 sdp2030101e4

10 100 000 Å 20 x 300 sdp2030101e5

10 1 000 000 Å 20 x 300 sdp2030101e6

10 10 000 000 Å 20 x 300 sdp2030101e7

20 100 Å 20 x 300 sdp2030201e2

20 500 Å 20 x 300 sdp2030205e2

20 1 000 Å 20 x 300 sdp2030201e3

20 10 000 Å 20 x 300 sdp2030201e4

20 100 000 Å 20 x 300 sdp2030201e5

20 1 000 000 Å 20 x 300 sdp2030201e6

20 10 000 000 Å 20 x 300 sdp2030201e7

112 PSS SDV Columns preparative, linearParticle size

[µm]Porosity

Dimension [mm]

Part Number

5 precolumn 20 x 50 sdp200505



5 linear S 20 x 300 sdp203005lis

5 linear M 20 x 300 sdp203005lim

5 linear XL 20 x 300 sdp203005lixl

10 linear S 20 x 300 sdp203010lis





113 PSS SDV Columns microParticle size

[µm]Porosity

Dimension [mm]

Part Number

3 precolumn 4.6 x 30 sdm05030350

3 50 Å 4.6 x 250 sdm0525035E1

3 100 Å 4.6 x 250 sdm0525031E2

3 500 Å 4.6 x 250 sdm0525035E2

3 1 000 Å 4.6 x 250 sdm0525031E3

3 10 000 Å 4.6 x 250 sdm0525031E4

3 100 000 Å 4.6 x 250 sdm0525031E5

3 1 000 000 Å 4.6 x 250 sdm0525031E6

3 linear S 4.6 x 250 sdm052503lis

3 linear M 4.6 x 250 sdm052503lim

110 PSS SDV Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number




5 50 Å 20 x 300 sdp2030055e1

5 100 Å 20 x 300 sdp2030051e2

5 500 Å 20 x 300 sdp2030055e2

5 1 000 Å 20 x 300 sdp2030051e3

5 10 000 Å 20 x 300 sdp2030051e4

5 100 000 Å 20 x 300 sdp2030051e5

5 1 000 000 Å 20 x 300 sdp2030051e6

10 50 Å 20 x 300 sdp2030105e1


9.2. GRAM Columns

114 GPC/SEC of polymers in polar organic solvents

Field of Application Applicability Polyurethane, Polyimide, Starches,

Celluloses, certain Polyamide, other polar polymers

Eluents DMF, DMAc, NMP, DMSO

SpecificationsMaterial Polyester copolymer network

Maximum Pressure50 -120 bar (725 - 1740 psi), depending on porosity


Maximum Flow Rate 2 ml/min (8 mm i.D.)

Particle Size 10 μm


Incompatibility drying and freezing


100 Å 300 - 60 000 Da

1 000 Å 1000 - 1 000 000 Da

3 000 Å 5 000 - 5 000 000 Da

10 000 Å 10 000 - 50 000 000 Da

linear 500 - 1 000 000 Da

Recommended Column CombinationLow molar mass: 100 Å + 100 Å

Med. mol. mass: 30 Å + 1 000 Å + 1 000 Å

High molar mass: 100 Å + 10 000 Å + 10 000 Å

115 PSS GRAM Columns analyticalParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 precolumn 8 x 50 ama080510

10 30 Å 8 x 300 ama0830103e1

10 100 Å 8 x 300 ama0830101e2

10 1 000 Å 8 x 300 ama0830101e3

10 3 000 Å 8 x 300 ama0830103e3

10 10 000 Å 8 x 300 ama0830101e4

10 linear 8 x 300 ama083010lin

Part Numbers

116 PSS GRAM Columns HighSpeedParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 100 Å 20 x 50 ams2005101e2

10 1 000 Å 20 x 50 ams2005101e3

10 3 000 Å 20 x 50 ams2005103e3

10 10 000 Å 20 x 50 ams2005101e4

10 linear 20 x 50 ams200510lin

117 PSS GRAM Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 precolumn 20 x 50 amp200510

10 30 Å 20 x 300 amp2030103e1

10 100 Å 20 x 300 amp2030101e2

10 1 000 Å 20 x 300 amp2030101e3

10 3 000 Å 20 x 300 amp2030103e3

10 10 000 Å 20 x 300 amp2030101e4

10 linear 20 x 300 amp203010lin

Applications

Calibration Curves

Sepe

rati

on o

ligom

ers

from

sal

t pe

ak


Eluent: DMAc; Temp.: 60° C; Detector: SECcurity RI;

Columns: SDV 10 µm 1 000 Å (red line); GRAM 10 µm 100 Å (blue line) (8 x 300 mm)

Met

hyl C

ellu

lose

Flow rate: 0.5 ml/min; Loading: 1 g/l, 50 µl;

Eluent: DMSO, LiBr 5 g/l; Temp.: 60° C; Detector: SECcurity RI;

Columns: GRAM 10 µm, 1 000 Å (8 x 300 mm) + precolumn

Sing

le P

oros

ity/

Line

ar C

olum

ns

Elue

nt: D

MF;

Cal

ibra

nts:

Po

ly(m

ethy

l met

hacr

ylat

e)

Elution Volume [ml]

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]

33

9.3. PFG Columns

118 GPC/SEC of Crystalline Polymers in Fluorinated Organic Solvents

Field of Application

Applicability Crystalline polymers, Polyesters, Nylons, Poly(lactide), POM, etc.

Eluents HFIP, TFE, other fluorinated solvents

SpecificationsMaterial Polar modified silica

Maximum Pressure150 - 200 bar (2180-2900 psi), depending on porosity



Particle Size 5, 7 μm


Incompatibility Drying and freezing


300 Å 1 000 - 300 000 Da

1 000 Å 10 000 - 1 000 000 Da

4 000 Å 10 000 - 3 000 000 Da

linear S 100 - 300 000 Da

linear M 100 - 1 000 000 Da

linear XL 100 -3 000 000 Da


Med. mol. mass: 100 Å + 1 000 Å


119 PSS PFG Columns analyticalParticle size

[µm]Porosity

Dimension [mm]

Part Number

7 precolumn 8 x 50 pfa080507

7 100 Å 8 x 300 pfa0830071e2

7 300 Å 8 x 300 pfa0830073e2

7 1 000 Å 8 x 300 pfa0830071e3

7 4 000 Å 8 x 300 pfa0830074e3

7 linear S 8 x 300 pfa083007lis

7 linear M 8 x 300 pfa083007lim

7 linear XL 8 x 300 pfa083007lxl

Part Numbers

120 PSS PFG Columns HighSpeedParticle size

[µm]Porosity

Dimension [mm]

Part Number

7 100 Å 20 x 50 pfs2005071e2

7 300 Å 20 x 50 pfs2005073e2

7 1 000 Å 20 x 50 pfs2005071e3

7 4 000 Å 20 x 50 pfs2005074e3

7 linear S 20 x 50 pfs200507lis

7 linear M 20 x 50 pfs200507lim

7 linear XL 20 x 50 pfs200507lxl

121 PSS PFG Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

7 precolumn 8 x 50 pfp200507

7 100 Å 20 x 300 pfp2030071e2

7 300 Å 20 x 300 pfp2030073e2

7 1 000 Å 20 x 300 pfp2030071e3

7 4 000 Å 20 x 300 pfp2030074e3

7 linear S 20 x 300 pfp203007lis

7 linear M 20 x 300 pfp203007lim

7 linear XL 20 x 300 pfp203007lxl

122 PSS PFG Columns microParticle size

[µm]Porosity

Dimension [mm]

Part Number

5 precolumn 4.6 x 30 pfm050305

5 100 Å 4.6 x 250 pfm0525051E2

5 300 Å 4.6 x 250 pfm0525053E2

5 1 000 Å 4.6 x 250 pfm0525051E3

5 linear S 4.6 x 250 pfm052505lis

5 linear M 4.6 x 250 pfm052505lim

Applications

Calibration Curves

Poly

(eth

ylen

e

ther

epht

hala

te)

Flow rate: 1 ml/min; Loading: 1.5 g/l, 100 µl;

Eluent: HFIP, K-TFAc 0.1 M; Temp.: 25° C; Detector: SECcurity RI;

Columns: PFG 7 µm 100 Å, 1 000 Å (8 x 300 mm) + precolumn

Sing

le P

oros

ity

Line

ar C

olum

ns

Elue

nt: H

FIP;

Cal

ibra

nts:

Po

ly(m

ethy

l met

hacr

ylat

e)

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]


126 PSS POLEFIN Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 precolumn 20 x 50 pop200510

10 1 000 Å 20 x 300 pop2030101e3

10 100 000 Å 20 x 300 pop2030101e5

10 1 000 000 Å 20 x 300 Pop2030101e6

10 10 000 000 Å 20 x 300 pop2030101e7

10 linear M 20 x 300 pop203010lim

10 linear XL 20 x 300 pop203010lxl

9.4. POLEFIN Columns

123 High Temperature (HT)-GPC/SEC of Polyolefins

Field of ApplicationApplicability Poly(ethylene), Poly(propylene),

other Polyolefins

Eluents TCB, o-DCB, Decalin

Specifications

MaterialModified styrene-divinylbenzene copolymer network






Incompatibility Water, alcohol, drying, and freezing


1 000 Å 100 - 60 000 Da

100 000 Å 1 000 - 1 000 000 Da

1 000 000 Å 1 000 - 4 000 000 Da

10 000 000 Å 5 000 - 30 000 000 Da

linear M 100 - 1 000 000 Da

linear XL 1 000 - 4 000 000 Da

Recommended Column CombinationLow molar mass: 100 Å + 1 000 Å + 100 000 Å

Med. mol. mass: 1 000 Å +100 000 Å + 1 000 000 Å

High mol. mass: 1 000 Å + 100 000 Å + 10 000 000 Å

124 PSS POLEFIN Columns analyticalParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 precolumn 8 x 50 poa080510

10 100 Å 8 x 300 poa0830101e2

10 1 000 Å 8 x 300 poa0830101e3

10 100 000 Å 8 x 300 poa0830101e5

10 1 000 000 Å 8 x 300 poa0830101e6

10 10 000 000 Å 8 x 300 poa0830101e7

10 linear M 8 x 300 poa083010lim

10 linear XL 8 x 300 poa083010lxl

Part Numbers

125 PSS POLEFIN Columns HighSpeed Particle size

[µm]Porosity

Dimension [mm]

Part Number

10 1 000 Å 20 x 50 pos2005101e3

10 100 000 Å 20 x 50 pos2005101e5

10 1 000 000 Å 20 x 50 pos2005101e6

10 10 000 000 Å 20 x 50 pos2005101e7

10 linear M 20 x 50 pos200510lim

10 linear XL 20 x 50 pos200510lxl

Applications

Long

ter

m s

tabi

lity


Eluent: TCB; Temp.: 145° C; Detector: SECcurity RI;

Columns: POLEFIN 10 µm 1 000 Å, 100 000 Å, 1 000 000 Å (8 x 300 mm) + precolumn

Poly

(eth

ylen

e)


Eluent: TCB; Temp.: 145° C; Detector: SECcurity RI;

Columns: POLEFIN 10 µm 1 000 Å, 100 000 Å, 1 000 000 Å (8 x 300 mm) + precolumn

Calibration Curves

Sing

le P

oros

ity/

Line

ar C

olum

ns

Elue

nt: T

CB;

Calib

rant

s:

Poly

(sty

rene

)

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]

35

10.1. SuPREMA Columns

127 Aqueous GPC/SEC of Neutral and Anionic Polymers

Field of ApplicationApplicability Neutral and anionic polymers,

(PEO, PEG, Pullulan, Dextran, Poly(acrylamide), Hyaluronic acid, Poly(acrylic acid), Carboxymethyl cellulose, etc.)

Eluents water (with salts/buffers, MeOH, ACN) pH: 1.5 – 13

SpecificationsMaterial Modified acrylate copolymer network



Maximum Flow Rate 2 ml/min (8 mm I.D.)

Particle Size 5, 10, 20 μm

Molecular Weight Range 100 to > 30 000 000 Da

Incompatibility Water, drying, and freezing


100 Å 100 - 100 000 Da

1 000 Å 100 - 1 000 000 Da

3 000 Å 1 000 - 3 000 000 Da

10 000 Å 1 000 - 10 000 000 Da

30 000 Å 5 000 - > 30 000 000 Da

linear S 100 - 100 000 Da

linear M 1 000 - 1 000 000 Da

linear XL 5 000 - 3 000 000 Da


Med. mol. mass: 30 Å + 1 000 Å + 1 000 Å


Applications

10| Columns for Aqueous Columns

Hyd

roxy

ethy

l sta

rch

GPC

/SE

C-M

ALL

S ac

c. E

uP


Eluent: water, NaN3 0.1 M; Temp.: 25° C; Detectors: SECcurity RI, SLD7000 MALLS;

Columns: SUPREMA LUX 10 µm 100 Å, 3 000 Å, 3 000 Å; (8 x 300 mm) + precolumn

Dex

tran

Alg

inat

e so

dium

sal

t


Eluent: water, NaN3 0.5 g/l; Temp.: 25° C; Detector: SECcurity RI;

Columns: SUPREMA 10 µm 30 Å, 1 000 Å (8 x 300 mm) + precolumn


Eluent: water, Na2HPO4 0.07 M; Temp.: 25° C; Detector: SECcurity RI;

Columns: SUPREMA 10 µm 3 000 Å (8 x 300 mm) + precolumn

Mol

ar M

ass

[MA

]

Conc

entr

atio

n [g

/l]

Det

ecto

r Si

gnal

[V]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Elution Volume [ml]

Elution Volume [ml]


Part numbers

128 PSS SuPREMA Columns analyticalParticle size

[µm]Porosity Dimension [mm]

Part Number

10 precolumn 8 x 50 sua080510

20 precolumn 8 x 50 sua080520

10 30 Å 8 x 300 sua0830103e1

10 100 Å 8 x 300 sua0830101e2

10 1 000 Å 8 x 300 sua0830101e3

10 3 000 Å 8 x 300 sua0830103e3

10 10 000 Å 8 x 300 sua0830101e4

10 30 000 Å 8 x 300 sua0830103e4

10 linear S 8 x 300 sua083010lis

10 linear M 8 x 300 sua083010lim

10 linear XL 8 x 300 sua083010lxl

20 100 Å 8 x 300 sua0830201e2

20 1 000 Å 8 x 300 sua0830201e3

20 3 000 Å 8 x 300 sua0830203e3

20 10 000 Å 8 x 300 sua0830201e4

20 30 000 Å 8 x 300 sua0830203e4

20 linear M 8 x 300 sua083020lim

20 linear XL 8 x 300 sua083020lxl

129 PSS SuPREMA Lux Columns analytical (for light scattering)

Particle size [µm]

Porosity Dimension [mm] Part Number

10 precolumn 8 x 50 sul080510

10 1 000 Å 8 x 300 sul0830101e3

10 3 000 Å 8 x 300 sul0830103e3

10 30 000 Å 8 x 300 sul0830103e4

10 linear XL 8 x 300 sul083010lxl

130 PSS SuPREMA Columns HighSpeedParticle size


Part Number

10 100 Å 20 x 50 sus2005101e2

10 1 000 Å 20 x 50 sus2005101e3

10 3 000 Å 20 x 50 sus2005103e3

10 10 000 Å 20 x 50 sus2005101e4

10 30 000 Å 20 x 50 sus2005103e4

Sing

le P

oros

ity

Colu

mns

Mix

ed B

ed/

Line

ar C

olum

ns

Elue

nt: a

queo

us b

uffe

r;

Calib

rant

s: P

ullu

lan

Elue

nt: a

queo

us b

uffe

r;

Calib

rant

s: P

ullu

lan

131 PSS SuPREMA Columns HighSpeed10 linear M 20 x 50 sus200510lim

10 linear XL 20 x 50 sus200510lxl

20 100 Å 20 x 50 sus2005201e2

20 1 000 Å 20 x 50 sus2005201e3

20 3 000 Å 20 x 50 sus2005203e3

20 10 000 Å 20 x 50 sus2005201e4

20 30 000 Å 20 x 50 sus2005103e4

20 linear M 20 x 50 sus200520lim

20 linear XL 20 x 50 sus200520lxl

132 PSS SuPREMA Columns preparativeParticle size


Part Number

10 precolumn 20 x 50 sup200510

20 precolumn 20 x 50 sup200520

10 30 Å 20 x 300 sup2030103e1

10 100 Å 20 x 300 sup2030101e2

10 1 000 Å 20 x 300 sup2030101e3

10 3 000 Å 20 x 300 sup2030103e3

10 10 000 Å 20 x 300 sup2030101e4

10 30 000 Å 20 x 300 sup2030103e4

10 linear S 20 x 300 sup203010lis

10 linear M 20 x 300 sup203010lim

10 linear XL 20 x 300 sup203010lxl

20 100 Å 20 x 300 sup2030201e2

20 1 000 Å 20 x 300 sup2030201e3

20 3 000 Å 20 x 300 sup2030203e3

20 10 000 Å 20 x 300 sup2030201e4

20 30 000 Å 20 x 300 sup2030203e4

20 linear M 20 x 300 sup203020lim

20 linear XL 20 x 300 sup203020lxl

133 PSS SuPREMA Columns microParticle size


Part Number

5 precolumn 4.6 x 30 sum050305

5 30 Å 4.6 x 250 sum0525053E1

5 100 Å 4.6 x 250 sum0525051E2

5 300 Å 4.6 x 250 sum0525053E2

5 1 000 Å 4.6 x 250 sum0525051E3

5 linear S 4.6 x 250 sum052505lis

5 linear M 4.6 x 250 sum052505lim

Calibration Curves

Elution Volume [ml] Elution Volume [ml]

Mol

ar M

asse

[D

a]

Mol

ar M

asse

[D

a]

37

135 PSS NOVEMA Columns analyticalParticle size


Part Number

10 precolumn 8 x 50 noa080510

10 30 Å 8 x 300 noa0830103e1

10 300 Å 8 x 300 noa0830103e2

10 1 000 Å 8 x 300 noa0830101e3

10 3 000 Å 8 x 300 noa0830103e3

10 10 000 Å 8 x 300 noa0830101e4

10 linear 8 x 300 noa083010lin

136 PSS NOVEMA Columns HighSpeedParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 300 Å 20 x 50 nos2005103e2

10 1 000 Å 20 x 50 nos2005101e3

10 3 000 Å 20 x 50 nos2005103e3

10 10 000 Å 20 x 50 nos2005101e4

10 linear 20 x 50 nos200510lin

137 PSS NOVEMA Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 precolumn 20 x 50 nop200510

10 30 Å 20 x 300 nop2030103e1

10 300 Å 20 x 300 nop2030103e2

10 1 000 Å 20 x 300 nop2030101e3

10 3 000 Å 20 x 300 nop2030103e3

10 10 000 Å 20 x 300 nop2030101e4

10 linear 20 x 300 nop203010lin

Part numbers

10.2. NOVEMA Columns


300 Å 100 - 300 000 Da

1 000 Å 1 000 - 1 000 000 Da

3 000 Å 1 000 - 1 600 000 Da

10 000 Å 5 000 - > 5 000 000 Da

Linear 100 - 500 000 Da


Med. mol. mass: 30 Å + 1 000 Å + 1 000 Å

High mol. mass: 30 Å + 3 000 Å + 3 000 Å

134 Aqueous GPC/SEC of Poly CationsField of ApplicationApplicability Cationic polymers, (Polymeric

Quarternary Ammonium Compounds, Poly (DADMAC), Poly(vinylpyridine), Chitosan, Poly(ethylene imine), etc.)

Eluents Water, water with salt/buffer, MeOH, ACN, TFA; pH: 1.5 - 13

Specifications

MaterialOH-functionalized methacrylate- copolymer-network







Calibration Curves

Sing

le P

oros

ity/

Line

ar C

olum

ns

Elue

nt: a

queo

us b

uffe

r; C

alib

rant

s:

Poly

(2-v

inyl

pyrid

ine)

Applications

Poly

(2-v

inyl

pyri

dini

um

brom

ide)


Eluent: water, NaC 0.1 M / TFAc 0.1%; Temp.: 25° C; Detector: SECcurity RI;

Columns: NOVEMA 10 µm, 3 000 Å (8 x 300 mm) + precolumn

Chit

osan

de

grad

atio

n st

udy


Eluent: water, NaC 0.1 M / TFAc 0.1%; Temp.: 25° C; Detector: SECcurity RI;

Columns: NOVEMA 10 µm 3 000 Å (8 x 300 mm) + precolumn

Elution Volume [ml]D

etec

tor

Sign

al [

V]

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]


Part numbers

139 PSS MCX Columns analyticalParticle size


Part Number

5 precolumn 8 x 50 mca080505



5 100 Å 8 x 300 mca0830051e2

5 500 Å 8 x 300 mca0830055e2

5 1 000 Å 8 x 300 mca0830051e3

10 1 000 Å 8 x 300 mca0830101e3

10 100 000 Å 8 x 300 mca0830101e5

10 1 000 000 Å 8 x 300 mca0830101e6

10 10 000 000 Å 8 x 300 mca0830101e7

20 10 000 000 Å 8 x 300 mca0830201e7

10.3. MCX Columns

138 Aqueous GPC/SEC of Sulfonated Polymers

Field of ApplicationApplicability Sulfonated Polyanions,

Poly(styrene sulfonate), Lignin sulfonate, Modified Starches, Acids, Alcohols, pectins, etc.)

Eluents Water, water with salt/buffer, MeOH, ACN; pH: 7 - 13

Specifications

MaterialSulfonated styrene-divinylbenzene copolymer-network




Particle Size 5, 10 and 20 μm




500 Å 100 - 35 000 Da

1 000 Å 100 - 70 000 Da

100 000 Å 1 000 - 1 000 000 Da

1 000 000 Å 10 000 - 2 000 000 Da

10 000 000 Å 10 000 - > 5 000 000 Da

Recommended Column CombinationLow molar mass: 1 000 Å + 1 000 Å

Med. mol. mass: 1 000 Å + 100 000 Å + 1 000 000 Å

High mol. mass: 1 000 Å + 100 000 Å + 10 000 000 Å

140 PSS MCX Columns HighSpeedParticle size

[µm]Porosity

Dimension [mm]

Part Number

10 1 000 Å 20 x 50 mcs2005101e3

10 100 000 Å 20 x 50 mcs2005101e5

10 1 000 000 Å 20 x 50 mcs2005101e6

20 10 000 000 Å 20 x 50 mcs2005201e7

141 PSS MCX Columns preparativeParticle size

[µm]Porosity

Dimension [mm]

Part Number

5 precolumn 20 x 50 mcp200505



5 100 Å 20 x 300 mcp2030051e2

5 500 Å 20 x 300 mcp2030055e2

5 1 000 Å 20 x 300 mcp2030051e3

10 1 000 Å 20 x 300 mcp2030101e3

10 10 000 Å 20 x 300 mcp2030101e5

10 1 000 000 Å 20 x 300 mcp2030101e6

20 10 000 000 Å 20 x 300 mcp2030201e7

Calibration Curves

Sing

le P

oros

ity

Colu

mns

Elue

nt: a

queo

us b

uffe

r; C

alib

rant

s:

Poly

(sty

rene

sul

fona

te)

sodi

um s

alt

Applications

Lign

in,

Lign

in s

ulfo

nate

, H

umic

aci

d


Eluent: water, NaOH 0.1 M; Temp.: 25° C; Detector: SECcurity RI;

Columns: MCX 10 µm 1 000 Å, 100 000 Å (8 x 300 mm) + precolumn

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]

39

10.4. PROTEEMA Columns

142 Aqueous GPC/SEC Separation of Proteins

Field of ApplicationApplicability Natural and synthetic Proteins,

Peptides, Enzymes, Gelatins/Collagens

Eluents Water, water with salt/buffer pH < 7

SpecificationsMaterial Special modified silica




Particle Size 3, 5 μm




Med. mol. mass: 300 Å + 300 Å

High molar mass: 100 Å + 1 000 Å

* based on protein molecular weight, for other water soluble macromolecules (e.g. pullulan) separation range ends at 50 000, 400 000, and 2 500 000 Da.


300 Å 1 000 - 1 200 000 Da

1 000 Å 1 000 - 7 500 000 Da

Part numbers

143 PSS PROTEEMA Columns analyticalParticle size

[µm]Porosity

Dimension [mm]

Part Number

5 precolumn 8 x 50 pra080505

5 100 Å 8 x 300 pra0830051e2

5 300 Å 8 x 300 pra0830053e2

5 1 000 Å 8 x 300 pra0830051e3

144 PSS PROTEEMA Columns microParticle size

[µm]Porosity

Dimension [mm]

Part Number

3 precolumn 4.6 x 30 prm050303

3 100 Å 4.6 x 250 prm0525031E2

3 300 Å 4.6 x 250 prm0525033E2

Calibration Curves

Sing

le P

oros

ity

Colu

mns

Elue

nt: a

queo

us b

uffe

r;

Calib

rant

s: P

ullu

lan

Applications

BSA

G

PC/

SEC-

MA

LLS

Flow rate: 0.5 ml/min; Loading: 1g/l, 20 µl; Eluent: Phosphate buffer pH = 6.6, NaCl 0.3 M;

Temp.: 25° C; Detectors: SECcurity RI + UV, SLD7000 MALLS;

Columns: PROTEEMA 5 µm 300 Å, 300 Å (8 x 300 mm) + precolumn

Prot

ein

mix

ture

Flow rate: 0.5 ml/min; Loading: 1g/l, 20 µl;

Eluent: water, NaCl 0.3 M; Temp.: 25° C; Detector: SECcurity RI;

Columns: PROTEEMA 5 µm 300 Å, 300 Å (8 x 300 mm) + precolumn

Elution Volume [ml]

Det

ecto

r Si

gnal

[V]

Elution Volume [ml]

Det

ecto

r Si

gnal

[U

V] a

t 28

0 m

m [

V]

Elution Volume [ml]

Mol

ar M

ass

[DA

]


11| GPC/SEC Application Guide

Polymer Column type

Solvent Temp [o C]

Calibration standards

Acrylic acid–Methyl methacrylate Copolymer

GRAM DMAc, LiBr 3 g/l + acetic acid 6 g/l

60 Poly(methyl methacrylate)

Acrylic acid-Methacrylate Copolymer



Alginate sodium salt SUPREMA Na2HPO4 0.07 M 25 Pullulan

Amino resin PFG DMAc LiCl 0.1 M 60 Poly(styrene)

Amylodextrine MCX Na2HPO4 0.07 M 25 Poly(styrene sulfonate) sodium salt

Anti-Human IgG PROTEEMA Phophate buffer pH 6.8

25 Pullulan

Apple juice MCX NaN3 0.05 % 25 Pullulan

Bitumen SDV THF 35 Poly(styrene)

BSA PROTEEMA Phosphatpuffer pH = 6,6 + NaCl 0,3 M

25 Pullulan

Butyl methacrylate-Styrene Copolymer

SDV THF 35 Poly(styrene)

Carboxymethyl cellulose

SUPREMA Na2HPO4 0.07 M 25 Pullulan

Carboxymethyl starch SUPREMA NaNO3 0,1 M 25 Pullulan

Carragenan SUPREMA LiNO3 0.1 M 25 Pullulan

Cellulose acetate GRAM DMAc LiBr 5g/l 60 Poly(styrene)

Cellulose nitrate SDV THF 25 Poly(styrene)

Cellulose triacetate SDV THF 25 Poly(styrene)

Chitin NOVEMA NaCl 0.1 M + TFAc 0.1 %

25 Pullulan

Chitosan NOVEMA NaCl 0.1 M + TFAc 0.1 %

25 Pullulan

Collagen PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Pullulan

Dextran SUPREMA NaNO3 0.1 M 25 Dextran

Dextran sulfate SUPREMA NaNO3 0.1 M 35 Pullulan

Dextran, oligomer MCX NaN3 0.05 % 25 Dextran

Dextrine SUPREMA NaNO3 0.1 M 25 Pullulan

Dimethylaminoethyl methacrylate-Metha-crylicester Copolymer



Epoxy resin SDV THF 25 Poly(styrene)

Ethylene-Methacrylate Copolymer


Ethylene-Propylene Copolymer

POLEFIN TCB 145 Poly(styrene)

Ethylene-Vinyl acetate Copolymer


Ferritin PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Protein Mixture

Gelatin PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Pullulan

Glyceride SDV THF 25 Poly(styrene)

Glycoprotein PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Protein Mixture

Guar gum SUPREMA NaNO3 0.1 M 25 Pullulan

Gum arabic SUPREMA NaNO3 0.1 M 25 Pullulan

Polymer Column type

Solvent Temp [o C]


Heparin SUPREMA NaNO3 0.1 M 25 Pullulan

Heparin (Pharmaeu-ropa, low molar mass)

PROTEEMA Na2SO4 28.4 g/L pH = 5

25 Heparin CRS 2

Heparin sulfate SUPREMA NaNO3 0.1 M 25 Pullulan

Humic acid MCX Na2HPO4 0.07 M 25 Poly(styrene sulfonate) sodium salt

Hyaluronic acid SUPREMA Na2HPO4 0.07 M 25 Pullulan

Hydraulic oil SDV THF 25 Poly(styrene)

Hydroxyethyl starch SUPREMA NaNO3 0.1 M 25 Hydroxy ethyl-starch

Hydroxypropyl cellulose

GRAM DMSO, LiBr 5 g/l 25 Poly(methyl methacrylate)

Hydroxypropyl cellulose ester

SDV THF 35 Poly(methyl methacrylate)

Hydroxypropyl cellulose ether


Insulin PROTEEMA L-Arginin, Water, Acetic acid and ACN

25 Protein Mixture

Iron Dextran SUPREMA NaNO3 0.1 M 25 Dextran

Isocyanate SDV THF 25 Poly(styrene)

Isopropyl methacrylate SDV THF 25 Poly(methyl methacrylate)

Lignin MCX NaOH 1 M 25 Poly(styrene sulfonate) sodium salt

Lignin sulfonate MCX Na2HPO4 0.07 M 25 Pullulan

Maltodextrine MCX NaN3 0.05 % 20 Pullulan

Melamin formaldehyde resin (MF)

GRAM NMP 60 Poly(styrene)

Methacrylic acid-Metha crylate Copolymer



Methyl cellulose GRAM DMSO, LiBr 5g/l 25 Poly(methyl methacrylate)

Methyl methacrylate-Styrene Copolymer


Naphthalin sulfonate MCX Na2HPO4 0.07 M 25 Pullulan

Norbenyl-Cyclodextrine Copolymer

GRAM DMF LiBr 5 g/l 25 Poly(methyl methacrylate)

Oleate SDV THF 25 HPLC calibration

Olive oil SDV THF 25 Poly(styrene)

Paraformaldehyde PFG HFIP, K-TFAc 0.05 M


Pectin SUPREMA NaNO3 0,1 M 25 Pullulan

Peptide PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Protein Mixture

Phenylene ether-Sulfone Copolymer


Poly(2-vinylpyridine) SDV THF, DEAEA 0,1% Poly(2-vinyl-pyridine)

Poly(2-vinylpyridine) NOVEMA NaCl 0.1 M + TFAc 0.1 %

Poly(2-vinyl-pyridin)

Poly(acrylamide) SUPREMA NaNO3 0.1 M 25 Pullulan /Poly(acrylamide) broad

Poly(acrylic acid) SUPREMA Na2HPO4 0.07 M 25 Poly(acrylic acid) sodium salt

Poly(acrylonitrile) SDV DMF, LiBr 5 g/l 60 Poly(methyl methacrylate)

41

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Polymer Column type

Solvent Temp [o C]


Poly(allylamine hydrochloride)

NOVEMA NaCl 0.1 M + TFAc 0.1 %

25 Poly(2-vinyl pyridine)

Poly(butadiene) (1.2 / 1.4)

SDV THF 25 Poly(butadiene 1.4)

Poly(butylene terephthalate) (PBT)

PFG HFIP, K-TFAc 0.1 M


Poly(carbonate) SDV THF 25 Poly(styrene)

Poly(DADMAC) NOVEMA NaCl 0.1 M + TFAc 0.1 %


Poly(dimethyl siloxane)

SDV Toluol 25 Polydimethylsi-loxan

Poly(ether sulfone) SDV DMAc, LiBr 5 g/l 80 Poly(methyl methacrylate)

Poly(ethyl methacrylate)


Poly(ethylene glycole) SDV THF 25 Poly(ethylene glycole)

Poly(ethylene glycole) SUPREMA NaN3 0.05 % 25 Poly(ethylene glycole)

Poly(ethylene imine) NOVEMA NaCl 0.1 M + TFAc 0.1 %


Poly(ethylene imide) GRAM DMAc LiBr 2 g/l +TRIS 2 g/l

60 Poly(styrene)

Poly(ethylene imine) PFG HFIP, K-TFAc 0.1 M


Poly(ethylene oxide) SUPREMA NaN3 0.05 % 25 Poly(ethylene oxide)

Poly(ethylene terephthalate) (PET)

PFG HFIP, K-TFAc 0.1 M


Poly(ethylene) POLEFIN TCB 145 Poly(styrene)

Poly(isobutylene) SDV THF 25 Poly(isobutylene)

Poly(isoprene) (1,4 / 3,4)

SDV THF 25 Poly(isoprene 1,4)

Poly(L-lactide) PFG TFE, K-TFAc 0.1 M 25 Poly(L-lactide)

Poly(L-lactide-glycolide)

PFG TFE, K-TFAc 0.1 M 25 Poly(L-lactide)

Poly(methacrylic acid) SUPREMA Na2HPO4 0.07 M 35 Poly(methacrylic acid)

Poly(methyl methacrylate)


Poly(n-butyl acrylate) SDV THF 25 Poly(t-butyl acrylat)

Poly(n-butyl methacrylate)

SDV THF 25 Poly(n-butyl-methacrylat)

Poly(n-propyl meth-acrylate)


Poly(oxymethylene) PFG HFIP, K-TFAc 0.1 M


Poly(phenyl acetylene) SDV THF 25 Poly(styrene)

Poly(propylene) (PP) POLEFIN TCB 150 Poly(styrene)

Poly(styrene sulfonic acid)

MCX Na2HPO4 0.07 M 25 Poly(styrene sulfonate) sodium salt

Poly(styrene) SDV THF 25 Poly(styrene)

Poly(styrene-b- butadiene-1,4)


Poly(styrene-b-glycidyl methacrylate)


Poly(t-butyl acrylat)e SDV THF 25 Poly(t-butyl-acrylat)

Poly(t-butyl methacrylate)

SDV THF 25 Poly(t-butylmetha-acrylat)

Polymer Column type

Solvent Temp [o C]


Poly(vinyl alcohol) SUPREMA NaNO3 0.1 M / MeOH (10 - 30 %*)

25 Pullulan/ Polyvi-nylalkohol breit

Poly(vinyl chloride) SDV THF 25 Poly(styrene)

Poly(vinylpyridinium bromide)

NOVEMA NaCl 0.1 M + TFAc 0.1 %


Poly(vinyl pyrrolidone) broad

GRAM DMAc, LiBr 0.1 %


Polyamide PFG HFIP, K-TFAc 0.1 M


Polyaminoamide GRAM DMF LiBr 5g/l 70 Poly(styrene)

Polycarbonaturethan (PCU)


Polyester PFG HFIP, K-TFAc 0.1M 25 Poly(methyl methacrylate)

Polyether, perfluori-nated

PFG HFIP, K-TFAc 0.1M 25 Poly(methyl methacrylate)

Polylactide PFG TFE, K-TFAc 0.1M 25 Poly(L-lactide)

Polyol SDV THF 25 Poly(styrene)

Polyolefin POLEFIN TCB 160 Poly(styrene)

Polysuccinamide SDV NMP, LiCl 0.1 M 25 Poly(methyl methacrylate)

Polysulfone SDV DMAc, LiBr 5 g/l


Polyurethane SDV THF 25 Poly(styrene)

Protein PROTEEMA Phophate buffer pH = 6.6 + NaCl 0.3 M

25 Protein-Mixture

Pullulan SUPREMA NaN3 0.05 % 25 Pullulan

Silicone- / Motor oils SDV THF 25 Poly(styrene)

Styrene-Butyl acrylate Copolymer


Styrene-Isoprene Copolymer


Trimethylammonium- Ethyl methacrylaet-Methacrylicester-Cl Copolymer

GRAM DMAc, LiBr 0.1% 60 Poly(methyl methacrylate)

Urea formaldehyde resin (UF)


Urea-melamin formal-dehyde resin (UMF)


Virus SUPREMA Phophate buffer pH 7.4

25 Pullulan

Xanthan SUPREMA Na2HPO4 0.07 M 25 Pullulan


12| Summary of PSS Columns Specifications

Applications Solvents Particle Size [μm]

Porosities [Å] Range MW [Da]

Organic GPC

SDV

Poly(styrene),

Poly(vinyl chloride),

Poly(carbonate),

Elastomers, Resins, etc.

THF, Toluene,

TCM, DCM

3

5

10

20

50 - 105, Linear S, M

50 – 106, Linear S, M, XL

50 – 107, Linear S, M, XL

100 - 107, Linear S, M, XL

100 - 1 M

100 - 3 M

100 - 30 M

500 - 30 M

SDV LuxSee Applications SDV, but for use with Light

Scattering Detection

THF, Toluene,

TCM, DCM

5

10

1 000 – 106, Linear M, XL

1 000 – 106, Linear M, XL

100 - 3 M

100 - 3 M

GRAMPolyurethanes, Polyimide, Starches, Cellulosics,

certain Polyamides, other polar polymers

DMF, DMAc,

NMP, DMSO10 30 – 10 000, Linear 100 - 50 M

PFGCrystalline polymers, Polyesters, Nylons,

Polylactides, POM, etc.

HFIP, TFE, other

fluorinated

solvents

5

7

100 - 1 000, Linear S, M

100 - 4 000, Linear S, M, XL

100 - 1 M

100 - 3 M

POLEFINPoly(ethylene),

Poly(propylene), other Polyolefins

TCB, oDCB,

Decalin10 1 000- 107, Linear M, XL 100 - 30 M

Aqueous GPC

SUPREMA

Neutral and anionic polymers, (PEO, PEG, Pullu-

lan, Dextran, Poly(acrylamide), Hyaluronic acid,

Poly(acryl acid), Carboxymethyl cellulose, etc.)

water

(with salts/buf-

fers, MeOH, ACN)

pH: 1.5 - 13

5

10

20

30 - 1 000, Linear S, M

30 - 30 000, Linear S, M, XL

100 - 30 000, Linear M, XL

100 - > 1 M

100 - > 30 M

100 - > 30 M

SUPREMA LuxSee Applications SUPREMA, but for use with

Light Scattering detection

water (with salts/

buffers, MeOH,

ACN) pH: 1.5 - 13

10 1 000 - 30 000, Linear XL 1 000 - > 30 M

NOVEMA

Cationic polymers, (Polymeric Quaternary

Ammonium Compounds, PolyDADMAc, Poly(vinyl

pyridines), Chitosan, Poly(ethylene imine), etc.)

water (with salts/

buffers, MeOH,

ACN, TFA) pH:

1.5 - 13

10 10 000, Linear 100 - > 5 M

MCX

Sulfonated Polyanions (Poly(styrene sulfonate),

Lignin (sulfonates), Modified Starches, Acids,

Alcohols, Pectins, etc.

water (with salts/

buffers, MeOH,

ACN) pH: 7 - 13

5

10

20

100 - 10³

1 000 - 107

107

100 - 70 K

100 - > 5 M

10 K - > 5 M

PROTEEMANatural and synthetic Proteins, Peptides,

Enzymes, Gelatins/Collagens

water (with salts/

buffers) pH: < 7

3

5

100 - 300

100 - 1 000

100 - 1.2 M

100 - 7.5 M

GPC/SEC Columns• For all Organic Eluents

• For all Aqueous Eluents

• For High and Low Molecular Weight

Synthetic and Bio-Polymers

• For Micro GPC/SEC up to Preparative Jobs

• HighSpeed Columns for fast Analysis

GPC/SEC Instruments• Complete Systems and Components

• Light Scattering Detectors

• Viscosity Detectors

• FTIR/MALDI Interfaces

• dn/dc Instrumentation

GPC/SEC Schools and Support • Full Services from Installation to Validation,

Operation, and Repair

• GPC/SEC and Software Training Schools

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• NetCommunity with Application and

Publication Downloads

Supplies and Services for Comprehensive Characterization of Natural and Synthetic Macromolecules

Reference Polymer Standards• GPC/SEC Standards and Kits

• Certified Reference Materials

• MALDI Kits

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• ReadyCal Kits

• Deuterated Polymers

• Tailor made Polymers and Copolymers

Software■ WinGPC UNITy MACROMOLECULAR

CHROMATOGRAPHy DATA SySTEM

• Light Scattering Modul for LALLS, RALLS,

TALLS, MALLS

• Viscosity Module

• Copolymer Module

• End-group Analysis Module

• 2-dimensional Chromatography Module

• Heparin Module

• LAN/Server Solutions

• Compliance Pack

■ PoroCheck SOFTWARE FOR PORE-

SIzE ANALySIS AND INVERSE GPC/SEC

Analytical services• Molar Mass Determination

• Branching/Structure Information

• Method Developement and Transfer

• Complete Product Deformulation

• Consulting

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