DPT Notes Final.1
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Downstream Process Technology – BT 402 Lecturer: Dr. Maulishree Agrahari SAP: Thirtha Poovaiah Lecture Number 21-22: Liquid liquid extraction and Distillation Lecture Number 36-37: Precipitaion methods with salts: Salting in and salting out, ammonium sulphate and PEG Lecture Number 42-43: Adsorptive chromatography: TLC, PC, Normal Phase and GC Lecture Number 44: HPLC Lecture Number 45: Gel permeatio n chromato graphy Lecture Number 46: Ion exchange chromatography Lecture Number 47: Membrane Chromatography Lecture Number 48: Electro- chromatography Lecture Number 49: Electrophor etic Separation: Native and SDS PAGE Lecture Number 50: Capillary Electrophor esis Lecture Number 51: Dialysis Lecture Number 52: Crystallization Liquid Liquid Extraction (LLE) Principle Liquid liquid extraction is a separat ing process that is based on the distribution of one or more components between two immiscible l iquids. Generally, one of the liquid phases is water and the other an organic solvent. Liquid liquid extraction is also known as solvent extraction, is especially suited for the processing of large capacities of feed. For this reason, LLE is frequently used in oil refining industry. The key principle underlying in LLE is often that the solubility differs significantly between the liquid, producing thermodynamic driving force for transfer from one phase to another. Feed phase contains a component, i, which is to be removed. Addition of a second phase (solvent phase) which is immiscible with feed phase but component i is soluble in both phases. Some of component i (solute) is transferred from the feed phase to the solvent phase. After extraction the feed and solvent phases are called the raffinate (R) and extract (E) phases respectively.
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