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ʹ ([WHQVLYH ZLWKLQKRVW GLYHUVLW\ LQ IHFDOO\ FDUULHG H[WHQGHGVSHFWUXP EHWDODFWDPDVH ͳ SURGXFLQJ Escherichia coli LPSOLFDWLRQV IRU WUDQVPLVVLRQ DQDO\VHV ʹ ͵ 6WRHVVHU 1 6KHSSDUG $( 0RRUH &( *ROXEFKLN 7 3DUU\ &0 1JHW 3 Ͷ 6DURHXQ 0 'D\ 13- *LHVV $ -RKQVRQ -5 3HWR 7($ &URRN ': :DONHU ͷ $6 DQG WKH 0RGHUQL]LQJ 0HGLFDO 0LFURELRORJ\ 000 LQIRUPDWLFV JURXS 1XIILHOG 'HSDUWPHQW RI &OLQLFDO 0HGLFLQH 8QLYHUVLW\ RI 2[IRUG 2[IRUG 8. ͺ 1,+5 %LRPHGLFDO 5HVHDUFK &HQWHU 8QLYHUVLW\ RI 2[IRUG2[IRUG 8QLYHUVLW\ ͻ +RVSLWDOV 1+6 7UXVW 2[IRUG 8. ͳͲ 'HSDUWPHQW RI &OLQLFDO 5HVHDUFK /RQGRQ 6FKRRO RI +\JLHQH DQG 7URSLFDO 0HGLFLQH ͳͳ /RQGRQ 8. ͳʹ 6FKRRO RI 7URSLFDO 0HGLFLQH DQG *OREDO +HDOWK 1DJDVDNL 8QLYHUVLW\ 1DJDVDNL ͳ͵ -DSDQ ͳͶ 0DKLGRO2[IRUG 7URSLFDO 0HGLFLQH 5HVHDUFK 8QLW %DQJNRN 7KDLODQG ͳͷ $QJNRU +RVSLWDO IRU &KLOGUHQ 6LHP 5HDS &DPERGLD ͳ 0LQQHDSROLV 9HWHUDQV $IIDLUV +HDOWK &DUH 6\VWHP 0LQQHDSROLV 0LQQHVRWD 86$ ͳ 'HSDUWPHQW RI 0HGLFLQH 8QLYHUVLW\ RI 0LQQHVRWD 0LQQHDSROLV 0LQQHVRWD 86$ ͳͺ ͳͻ &RUUHVSRQGLQJ DXWKRU ʹͲ (PDLO IRU FRUUHVSRQGHQFH QLFROHVWRHVVHU#QGPR[DFXN ʹͳ ʹʹ 5XQQLQJ WLWOH +LJK GLYHUVLW\ LQ IHFDOO\ FDUULHG (6%/E. coli ʹ͵ ʹͶ ʹͷ JCM Accepted Manuscript Posted Online 22 April 2015 J. Clin. Microbiol. doi:10.1128/JCM.00378-15 Copyright © 2015, American Society for Microbiology. All Rights Reserved. on May 25, 2020 by guest http://jcm.asm.org/ Downloaded from

Transcript of Downloaded from on April 4, 2020 by guest · tzv vdpsohg ll wkh67 l h wkhvdph67exwvhsdudwhge\odujh...

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Escherichia coli

E. coli

JCM Accepted Manuscript Posted Online 22 April 2015J. Clin. Microbiol. doi:10.1128/JCM.00378-15Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Escherichia coli

E. coli

E. coli

E. coli

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E. coli

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Escherichia coli

E. coli

E. coli

E. coli

E. coli

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Staphylococcus aureus

E. coli

Sample description

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Laboratory methods

E. coli

E. coli

DNA extraction and sequencing

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Sequence read processing and data analysis

E. coli

de novo

in silico de

novo

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E. coli

Clostridium difficile

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E. coli

E. coli

E. coli

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E.

coli de novo

In silico multi-locus sequence (MLST) typing

silico E. coli

fumC purA recA

Determination of genotypes accounting for cefpodoxime resistance

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bla

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Analysis of within-host E. coli core and accessory genome diversity

E. coli

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Significance of the study and implications for transmission studies of intestinally

carried CTX-M-positive E. coli

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E. coli

E. coli

E. coli

bla

E. coli

Clostridium difficile

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in silico

E. coli

Limitations of the study

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E. coli

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E. coli

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bla bla

bla

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bla

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bla

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Escherichia coli

bla

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Escherichia

Enterobacter cloacae

Escherichia coli

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Klebsiella pneumoniae

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Klebsiella

pneumoniae

Escherichia coli

Escherichia coli

Staphylococcus aureus

Staphylococcus aureus

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Escherichia coli

C. difficile

Escherichia coli

Escherichia coli Klebsiella pneumoniae

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Escherichia coli

Escherichia coli

Escherichia coli

Clostridium difficile

Escherichia coli

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Escherichia coli

Escherichia coli

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