Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.
-
Upload
simon-lawrence -
Category
Documents
-
view
214 -
download
1
Transcript of Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.
![Page 1: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/1.jpg)
Dong Zheng
Addiction Therapy-2014Chicago, USA
August 4 - 6, 2014
![Page 2: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/2.jpg)
Your company slogan
LOGO
The Protective Effect of Astaxanthin on Fetal Alcohol Spectrum Disorder in Mice
DONG ZHENG, M.D. ,PH.D.
Department of Neurology,
Guangzhou Brain Hospital, China
![Page 3: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/3.jpg)
Background
Mechanisms of Oxidative Stress Related FASD and Prevention of AST
Intervention of AST on the TLRs system
1
2
3
4 Conclusions
![Page 4: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/4.jpg)
FAS
Intrauterine growth retardation (IUGR)
Craniofacial malformations
Physical and mental retardation
Background
1973 Jones and Smith
Cardiac septal defects
![Page 5: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/5.jpg)
Background
FASD -- Foetal Alcohol Spectrum Disorders
![Page 6: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/6.jpg)
Alcohol
ASTROS
Mechanisms of Oxidative Stress Related FASD and Prevention of AST
![Page 7: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/7.jpg)
Animals and experimental treatment
• Wild type female mice
• gestational day
• G0: gestational day
• G7: AST
• G8:ethanol and AST
• G9:for RT-PCR, Western-blot , ELISA;
• G10:for Morphology study
G9 PCR,ELISAG10:Morphology
ethanol
G 0
G8
Ethanol groups control groups
G7
AST
AST
Morphology assessment of embryos
AST Blank
AST Blank
6
![Page 8: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/8.jpg)
AST prevented ethanol-induced developmental retardation in mouse embryos
7
![Page 9: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/9.jpg)
Down-regulation of neural marker genes
8
![Page 10: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/10.jpg)
Mechanisms of oxidative stress related FASD and prevention of AST
A. malondialdehyde(MDA)
B. hydrogen peroxide(H2O2)
C. glutathione peroxidase per oxidase(GPx)
9
![Page 11: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/11.jpg)
Intervention of AST on the TLRs system
10
![Page 12: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/12.jpg)
Intervention of AST on the TLRs system
A. AST inhibited ethanol-induced production of TNF-a
B. AST inhibited ethanol-induced production of and IL-1bin embryos
10
![Page 13: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/13.jpg)
Discussion
• AST pretreatment protected embryos from maternal ethanol-induced neurodevelopmental retardation and down-regulation of neural marker genes.
• Inflammation played a role in mechanisms of FASD.
• TLR4 is distinct expression in embryos and related to inflammation
![Page 14: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/14.jpg)
Conclusions
Our results demonstrated a protective effect of
astaxanthin on fetal alcohol spectrum disorder
Oxidative stress and toll-like receptor signaling
associated inflammatory reaction were involved
in this process.
![Page 15: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/15.jpg)
Acknowledgements
![Page 16: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/16.jpg)
![Page 17: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/17.jpg)
Thank you !
![Page 18: Dong Zheng Addiction Therapy-2014 Chicago, USA August 4 - 6, 2014.](https://reader035.fdocuments.in/reader035/viewer/2022070406/56649e0d5503460f94af6642/html5/thumbnails/18.jpg)
Addiction Therapy – 2015 Website:
addictiontherapy.conferenceseries.com
Meet the eminent gathering once again at
Addiction Therapy-2015Florida, USA
August 3 - 5, 2015