Dolly sheepDolly sheepDirected by : nooralhuda Directed by : nooralhuda

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cloningcloning cloningcloning  is the creation of a is the creation of a

genetically identical copygenetically identical copy  

How ?How ?

Why was Dolly createdWhy was Dolly created?? In mice it was possible to genetically In mice it was possible to genetically

modify these cells.modify these cells.

But they found that the process of But they found that the process of cloning the best results for the sheep cloning the best results for the sheep and not the mouse and not the mouse

birthbirth where the cell nucleus from an adult cell is transferred where the cell nucleus from an adult cell is transferred

into an unfertilised oocyte The hybrid cell is then into an unfertilised oocyte The hybrid cell is then stimulated to divide by an electric shock, and when it stimulated to divide by an electric shock, and when it develops into a blastocyst it is implanted in a surrogate develops into a blastocyst it is implanted in a surrogate mother.mother.

lifelife Dolly lived for her entire life at the Roslin Institute. Dolly lived for her entire life at the Roslin Institute.

She was born in April 1998. The next year Dolly produced She was born in April 1998. The next year Dolly produced twin lambs Sally and Rosie, and she gave birth to triplets twin lambs Sally and Rosie, and she gave birth to triplets Lucy, Darcy and Cotton in the year after that.Lucy, Darcy and Cotton in the year after that.

  In the autumn of 2001, at the age of five, Dolly developed In the autumn of 2001, at the age of five, Dolly developed arthritisarthritis and began to walk stiffly, but this was successfully  and began to walk stiffly, but this was successfully treated with treated with anti-inflammatoryanti-inflammatory drugs. drugs.

deathdeath On 14 February 2003, Dolly was had a On 14 February 2003, Dolly was had a

progressive lung disease and severe arthritis.progressive lung disease and severe arthritis.

A Finn Dorset such as Dolly has a life A Finn Dorset such as Dolly has a life expectancy of around 11 to 12 years, but expectancy of around 11 to 12 years, but Dolly lived to be only six years of age.Dolly lived to be only six years of age.

A post-mortem examination showed she had A post-mortem examination showed she had a form of lung cancer called Jaagsiekte, a form of lung cancer called Jaagsiekte, which is a fairly common disease of sheep which is a fairly common disease of sheep and is caused by the retrovirus JSRV. and is caused by the retrovirus JSRV.

referencereference 1-1- McLaren A (2000). "Cloning: pathways to a pluripotent  McLaren A (2000). "Cloning: pathways to a pluripotent

future". future". ScienceScience  288288 (5472): 1775–80.  (5472): 1775–80. doidoi::10.1126/science.288.5472.177510.1126/science.288.5472.1775..PMIDPMID  1087769810877698..

2-2-aa  bb  "Dolly the sheep clone dies young""Dolly the sheep clone dies young", BBC News, Friday, 14 , BBC News, Friday, 14 February 2003February 2003

3-3-  "Is Dolly old before her time?""Is Dolly old before her time?". . BBC NewsBBC News (London). 27 May  (London). 27 May 1999. Retrieved 2009-10-04.1999. Retrieved 2009-10-04.

4-4-httphttp://://enen..wikipediawikipedia..orgorg//wikiwiki//Dolly_Dolly_((sheepsheep))

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