Does a Rotational Enrichment Program Improve the Well-being of Sprague Dawley Rats?
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Does a Rotational Enrichment Does a Rotational Enrichment Program Improve the Well-being of Program Improve the Well-being of
Sprague Dawley Rats?Sprague Dawley Rats?
Debra L. Hickman, DVM, MS, DACLAM
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ENRICHMENT FOR RATSENRICHMENT FOR RATS
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Structural & SocialStructural & Social
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Nesting/Foraging/GnawingNesting/Foraging/Gnawing
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• Can we improve rat well-being by implementing a rotational enrichment scheme?
• We know this is important in other highly social species (e.g. dogs, NHP)
• Proposed use of behavioral and immunological assessment to measure rat well-being
Research QuestionResearch Question
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SPATIAL COGNITIVE BIAS ASSAYSPATIAL COGNITIVE BIAS ASSAY
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Spatial Cognitive Bias AssaySpatial Cognitive Bias Assay
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LEARNING TIMES AND PATH OF LEARNING TIMES AND PATH OF EXPLORATION ANALYSISEXPLORATION ANALYSIS
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PASSIVE BEHAVIORAL ASSESSMENTPASSIVE BEHAVIORAL ASSESSMENT
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Materials and MethodsMaterials and Methods• Male Sprague Dawley rats• Train to Spatial Cognitive Bias Assay• Test on Spatial Cognitive Bias Assay– Initial– Repeat in 3 months– Reverse the location of the rewarded pot and
measure learning and path
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Experimental DesignExperimental Design• All individually housed with no enrichment during training• After learn spatial discrimination task:
– Move to treatment condition– Initiate testing 24-36 hours later
• Repeat cognitive bias testing 3 months later• Reverse rewarded vs nonrewarded locations and measure
days to learn and path of exploration
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Treatment GroupsTreatment Groups• Controls:– Social isolation– Pair housed
• Intracage shelter• Nesting material• Daily provision of food
treat
• Shelter, nesting material, and daily food treat (“superenriched”)
• Weekly rotation of shelter, nesting material, and daily food treat
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RESULTSRESULTS
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AcknowledgementsAcknowledgements
• Melissa Swan• R’nld Wheeler• Tiffany Riddle• Jessica Peveler• Jesus Bazan
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Questions?