Forensic DNA Laboratory of

INP

LABORATORIUM DNA FORENSIK

BIDDOKPOL PUSDOKKES POLRI

1990 2005Dr. Christanto TH, dr. Slamet Poernomo, drg. Alphonsus Q, dr. Lukman Hakim as poineer of the DNA

laboratory at Biddokpol Pusdokkes Polri

Serology : pretest; Dot Blot (1992); Organic Isolation Validation (1993);HLA DQ-A(early 1994);

PolyMarker (mid 1994); D1S80 (late 1994); SA Gel Electrophoresis.

2006 March 26, 2007

Rehabilitation and development of the new DNA laboratory built under collaboration of INP and AFP,

on March 26, 2007 Kapolri and AFP Police Commissioner officially opened the new DNA laboratory

under the name

LABORATORIUM DNA FORENSIK BIDDOKPOL PUSDOKKES POLRI

March 2007 TODAYValidation lab flow charts and standard operating procedures as well as the equipments and training

for human resources development program as part of the collaboration between INP AFP in

Canberra, Australia. Increasing the number of lab technicians with biologist or medical background.

Completion of SOP and manuals for the lab both in technical and quality terms, as part of the next

phase of the lab target, that is being internationally accredited, with the supervision and technical

support from AFP until 2009.

Photos of the new lab with the official

opening by kapolri & AFP Police

Commissioner

CE RoomChemical &

Prep Room

Extraction Room Examination Room 2

Amp

Room

Pre Amp

Room

Examination

Room 1Histology

Storage

Adminis

tration

II nd Flour DNA Lab Biddokpol Pusdokkes Polri

Server &

Power

Clothes

Flow of Samples

LABORATORIUM DNA FORENSIK

BIDDOKPOL PUSDOKKES POLRI

Bilateral Co-operation1. The Eijkman Institute for Molecular Biology

2. Australian Federal Police

Regional Co-operationsADNAP/REAFD

International Co-operationsICPO/INTERPOL DNA Unit and DVI standing committee

LABORATORIUM DNA FORENSIK

BIDDOKPOL PUSDOKKES POLRI

Most of the cases we handled were criminal cases and some were family

disputes.

Types of cases:

Mutilation.

Murder / serial murder

Traces.

Paternity.

Missing person.

Terrorism.

Methods used for DNA extractions:

Chelex extraction for blood(dried blood from evidence or crime scene, semen, buccal swabs, hair root and trace samples,

reference blood samples, soft tissue samples.

Organic Extraction for bone samples

victim

tools

suspect

Court

Crime scene

Paternity

DVI

W???

Fine out :

Apropriate sample

Apropriate Another cyrcle

32 LCN

28 Norma

Microcon

Genetic analyzer software

Use Population Database

Excel counting for Probability

All Equ.

All Procedures.

Pretest : Otol, AP.

Sampling : blood, swab, bone, hiroot, cig but, sperm, type-lift,

tooth, degraded.

Extraction : Chelex, organic.

Quant.

Amp.

Electrophoresis.

Typing.

Report.

fillingstoragewashingsterilewaterwasteworksh

eetsecurity MSDSfireemergency

aiddll...........................

INP-AFP-UC Scientists Training Course

University of Canberra

June 2007

Sub-population Abbr. N

Malay MT 100

Javanese JW 80

Javanese (Diabetic Patients) JV 20

North Sumatra (Batak Toba) TBS 20

North Sumatra (Batak Karo) KR 20

Mentawai MTW 20

Lembata LEM 20

East Flores (Larantuka) LAR 20

West Flores (Rampasasa) RAM 20

Central Flores SOA 20

Alor ALO 20

East Sumba (Sumba Timur) SBT 20

South Sulawesi (Toraja) TRJ 20

Kalimantan (Dayak-Benuaq) DY 20

NINDIAN

OCEAN

PACIFIC

OCEAN

SOUTH

CHINA SEA

TIMOR

SEA

ARAFURA

SEA

PNG

AUSTRALIA

CELEBES

SEA

1. Central Flores (SOA)

2. West Flores (RAM)

3. East Flores (LAR)

4. Lembata (LEM)

5. Alor (ALO)

6. East Sumba (SBT)

7. South Sulawesi (TRJ)

8. Kalimantan (DY)

9. North Sumatra (KR)

10. North Sumatra (TBS)

11. Mentawai (MTW)

12. Javanese (JW)

13. Javanese (JV)

14. Malay

12 3,45

6

7

8

9

10

11

12,13,14

Neighbour-joining tree to represent full distance matrix

Javanese population departs from others and logical dispersion of remaining groups on the basis of geography

MDS plot to represent full distance matrix. Populations that are genetically close plot close together in 2-D space. Again, Javanese population departs from the others whilst the remaining groups tend towards clusters that have a geographical basis.

Malay

JW

JV

DY

TRJ

MTW

TBS

KR

LEM

SBT LAR

RAM

ALO

SOA

Nusa Tenggara

Nth West Sumatra,

Mentawai

Kalimantan, Java,

South Sulawesi

The source

Drive me to the reference forensic DNA lab.

The development of the science on DNA polymorphism, its identification technologies, and the related biostatistics leads DNA to become an almost perfect tool in the field of forensic identification. It could be stated that DNA use for personal identification is already recognized and admissible worldwide for judicial purposes.

Budi Sampurna

DNA legislation

The use of DNA for judicial purposes in Indonesia has

been applied for more than one decade, particularly in

criminal and in disputed paternity cases.

When a mass disaster occurred in Bali in 2002 due to

bomb-explosions, DNA identification was applied and

contributed in 22.27% cases as a primary method

(n=202), while dental record was 55.44% and

fingerprint was 11.38%.

However, Indonesia doesnt have any legislation on the

application of DNA and its related science for judicial

purposes.

Budi Sampurna

DNA legislation

The importance of DNA legislation in a country is to

regulate the application of DNA testing in order to

ensure and guarantee that it would be used properly

for the sake of peoples welfare and justice.

Legislation on DNA, at one side must be able to

regulate its optimal usage for delivering clinical

genetic services, law enforcement uses for forensic

purposes in both criminal and civil courts, insurance,

employment, sport, immigration and parentage and

kinship testing; and on the other side to protect

privacy, unfair discrimination and high ethical

standard Budi Sampurna

DNA legislation