DNA to RNA and Protein Synthesis 2017 - Weebly
Transcript of DNA to RNA and Protein Synthesis 2017 - Weebly
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6.C: Students will…
explain the purpose and process of
transcription and translation using
models of DNA and RNA
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DNA mRNA Protein
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DNA is found in the nucleus, but making a protein occurs at the ribosome (either floating in the cytoplasm or attached to the rough ER)
The DNA code must be copied and moved from the nucleus out to the cytoplasm (this is called transcription)
Once in the cytoplasm, the code must be read so amino acids (building blocks of protein) can be assembled (this is called translation)
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DNA cannot fit through the nuclear pores,
and therefore cannot leave the nucleus to
go to the ribosomes
In order to get the DNA code out of the
nucleus and into the cytoplasm, it must be
turned into another nucleic acid called RNA
RNA stands for ribonucleic acid
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RNA is different from DNA because it
contains the sugar ribose (instead of
deoxyribose)
RNA does not contain the base thymine
– instead it has the base uracil
› A goes with U
› C goes with G
The RNA molecule
is single-stranded
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Messenger RNA (mRNA) – copies the
DNA code for a gene and carries that
information to the ribosomes outside the
nucleus
Transfer RNA (tRNA) – transfers (carries)
amino acids to the ribosomes, where
proteins are made
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Long, straight chain of nucleotides
Made in the nucleus – copies the DNA code
for a gene and then exits out the nuclear
pores and goes to a ribosome
Contains the nitrogen bases A, G, C, and U
Carries information to build a specific protein
A sequence of 3 bases is called a codon
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Transfers (carries) amino acids to the
ribosomes, where proteins are made
Single-stranded molecule
with an attachment site at
one end for an amino acid
The other end has three
nucleotide bases called
the anti-codon
(complementary to the
codon)
Transfer = taxi
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A codon designates a specific amino acid
There are 20 amino acids created from
the 64 possible codons
One codon tells the ribosome to start
translating (AUG), and three tell the
ribosome to stop translating (UAA, UAG, or
UGA)
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The production of a polypeptide chain
› Polypeptide chain = protein
› A string of amino acids held together with
peptide bonds
DNA mRNA Protein
› The mRNA is read in sets of 3 bases (codon)
Two steps in protein synthesis – transcription
and translation
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The process of copying the sequence of
one strand of DNA (the template strand)
and turning that information into mRNA
Requires the enzyme RNA polymerase
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Practice: what would be the
complementary RNA strand for the
following DNA sequence?
DNA 5’ – GCGTATG – 3’
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Practice: what would be the
complementary RNA strand for the
following DNA sequence?
DNA 5’ – GCGTATG – 3’
RNA 3’ – CGCAUAC – 5’
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During transcription, RNA polymerase binds
to DNA and separates the DNA strands
RNA polymerase then uses one strand of
DNA as a template to assemble
nucleotides into RNA
› Promoters are regions on DNA that show where
RNA polymerase must bind to begin transcription
› Specific base sequences act as signals to stop
transcription (termination signal)
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Also known as RNA splicing
After DNA is transcribed into RNA, it must
be edited to make it functional
› Introns – non-functional segments of RNA
(they are taken out of the chain)
› Exons – coding segments of RNA (they are
left in)
mRNA leaves the nucleus through the
nuclear pores and goes to the ribosome
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The process of decoding the mRNA and
turning it into a polypeptide chain
(protein)
Ribosomes read
mRNA 3 bases
(one codon) at
a time
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Step 1 – Initiation
› The mRNA start codon (AUG) attaches to the
ribosome
› The first tRNA molecule (with the first amino
acid attached) moves into the P site
› A second tRNA molecule enters the A site
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Step 2 – Elongation› Peptide bonds attach the amino acids together
› Once complete, the tRNA in the P site moves to
the E site (exit site) and detaches
› The ribosome moves down the mRNA,
continuing to attach amino acids with peptide
bonds
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Step 3 – Termination› When the ribosome reaches one of the three
stop codons, translation stops
› The ribosome releases the amino acid chain
(polypeptide)
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End Product
› The end product of protein synthesis is a
protein (primary structure)
› A sequence of amino acids bonded
together with peptide bonds (also called a
polypeptide)
aa1
aa2 aa3 aa4aa5
aa200
aa199
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Once it has been released, the polypeptide
folds into its appropriate shape
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There are 20 amino acids and 64 codons
This means several different codons can specify the same amino acid
There are several cases in which the third base of the codon can be changed, but the same amino acid will be coded for (silent mutation)› Means the point mutation won’t affect the
final protein
› Ex: mRNA GCU, GCA, GCG, GCC all code for the amino acid alanine
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A U G G G C U C C A U C G G C G C A U A AmRNA
start
codon
codon 2 codon 3 codon 4 codon 5 codon 6 codon 7codon 1
methionine glycine serine isoleucine glycine alanine stop
codonprotein
Primary structure of a protein
aa1 aa2 aa3 aa4 aa5 aa6
peptide bonds
T A C C C G A G G T A G C C G C G T A T TDNA
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