DNA STRUCTURE Blueprint of LifeStandard 5a-c 5a. DNA & RNA DNA is a double helix made of a sugar-...
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Transcript of DNA STRUCTURE Blueprint of LifeStandard 5a-c 5a. DNA & RNA DNA is a double helix made of a sugar-...
![Page 1: DNA STRUCTURE Blueprint of LifeStandard 5a-c 5a. DNA & RNA DNA is a double helix made of a sugar- phosphate backbone with complimentary bases paired.](https://reader033.fdocuments.in/reader033/viewer/2022051113/56649d095503460f949dbfce/html5/thumbnails/1.jpg)
DNA STRUCTUREBlueprint of Life Standard 5a-c
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5a. DNA & RNA
DNA is a double helix made of a sugar-phosphate backbone with complimentary bases paired in middle
Nucleotide (sugar, phosphate, & base)
A pairs with T C pairs with G
RNA vs. DNA Difference DNA RNA
# of Strands Double Single
Bases thymine uracil
Sugar Deoxyribose Ribose
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Questions
1. The monomers that are put together to make nucleic acids are called: A) Nucleotide B) Phosphate C) Base D) Sugar
A. Nucleotides
C. Nucleic Acids2. DNA & RNA are made up of which type of macromolecule?
A) CarbohydrateB) LipidsC) Nucleic AcidsD) Proteins
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DNA RNA
-________ stranded - ______ stranded- ____________ (sugar) - _______ (sugar)- thymine (nitrogenous base) - _____ (nitrogenous base)
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1. DNA or RNA?2. Identify the bases in strand B
GTGACC
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5b. DNA Replication
DNA Replication makes a copy of DNA before cell division (mitosis or meiosis)
DNA replication is semi-conservative Each parent strand is a
template for new daughter strand.
DNA has anti-parallel strands They run in opposite directions
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Enzymes carry out replication 1) Helicase – unzips DNA 2) Primase – starts
replication 3) Polymerase – matches A-
T, C-G to make new strands 4) Ligase – glues lagging
strand fragments together
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Question
What does semi-conservative mean? A) Strands are anti-parallel. B) The old strands serve as templates for
the new DNA. C) The old strands are lost.
What enzyme matches DNA base pairs (A-T, C-G)?
A) HelicaseB) PrimaseC) PolymeraseD) Ligase
B. Old strand is conserved
C. Polymerase
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5c. Genetic Engineering
Recombinant DNA contains DNA from 2+ organisms.
Restriction enzymes cut out gene from DNA
Vectors (bacteria & viruses) can be used to insert new gene into cell.
Uses: 1) Make medicines
Ex: insulin, human growth hormone 2) Food Crops
Ex: pest-resistant, larger fruits/veggies
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PROTEIN SYNTHESISDNA > mRNA > ribosome > protein
Standard 4a & b
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4a. Protein Synthesis
Protein Synthesis = making proteins
1. Transcription = DNA > mRNA
DNA is transcribed (copied) into messenger RNA (mRNA) to leave the nucleus
DNA is too big, it does not leave the nucleus
mRNA carries the info in DNA out of the nucleus to the ribosomes in the cytoplasm
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4a. Protein Synthesis
2. Translation = mRNA > protein mRNA is translated into a protein
by a ribosome
Codon = 3 letter “words” on mRNA Ex. U G G U C A A U C
Transfer RNA (tRNA) matches each codon and transfers the correct amino acids
Amino acids add together to make a polypeptide chain, which becomes a protein
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4b. Build a Protein
1. Transcription DNA > mRNA Base Pairing Rules
DNA: A-T C-G mRNA: A-U C-G
Transcribe the DNA template strand into mRNA
DNA : C T G T A C G G A template strand
mRNA: G A C A U G C C U
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A base sequence of DNA is shown below.
ACAGTGC How would the base sequence be
coded on mRNA? A) TGTCACG B) GUGACAU C) UGUCACG D) CACUGUA
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4b. Build a Protein
2. Translation: mRNA > protein
Use the codon tableCodon Amino
Acid
AUG
UGU
ACG
GAC
UAA, UAG, UGA
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MUTATIONS & PROTEINSChanges in DNA std. 4c-e
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4c. Mutations
Mutation = permanent change in the DNA base sequence
Mutations can be good, neutral, or bad 1.Single base change – may or may not have
an effect (ex: A C)What would happen if GGG changed to GGC?
both= Gly so nothing…What would happen if UAC changed to UAG?
stops protein production so effect could be major…
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2. An insertion or deletion changes the reading “frame” The fat cat ate the rat The atc ata tet her at Major effects!
Mutations occurs in sperm or egg – passed to offspring = disease Tay Sachs disease, sickle-cell anemia, muscular dystrophy
Mutations occurs in regular body cells they may cause cancer
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4d. Cell Specialization
All cells in your body have the SAME DNA
Only genes need by that cell are expressed.
Each cell only expresses the portion of the DNA containing the genetic information for the proteins required by that cell at that time.
The remainder of the DNA is not expressed
Example: The cells of in your skin have the
DNA that codes for your eye color protein. They just don’t use it
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4e. Proteins
20 amino acids make up all proteins. Your body creates many different proteins by
changing the number and sequence of amino acids
Proteins vary from about 50 to 3,000 amino acids in length.
The types, sequences, and numbers of amino acids used determine the type of protein produced.
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Hemoglobin – 574 aa Insulin – 51aa
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One human disease is caused by a change in one codon in a gene from GAA to GUA. This disease is the result of:
A a mutation.B a meiosis error.C crossing-over.D polyploidy.
Although there are a limited number of amino acids, many different types of proteins exist because the
A. size of a given amino acid can vary. B. chemical composition of a given amino acid can vary. C. sequence and number of amino acids is different. D. same amino acid can have many different properties.
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Mutations within a DNA sequence are:
A. natural processes that produce genetic diversity.
B. natural processes that always affect the phenotype.
C. unnatural processes that always affect the phenotype.
D. unnatural processes that are harmful to genetic diversity.
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CHROMOSOMESWound up DNA Standard 2e-f
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2e. Chromosomes
Write really small!!! Chromosome -
wound-up DNA containing genes
Sister chromatids are identical copies held together by a centromere
Draw & label a chromosome on the right margin.
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2e. Homologous Chromosomes Homologous chromosomes – have the
SAME genes at the SAME locations One came from mom, one from dad
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2f. Sex-determination
Karyotype – chart shows all the homologous pairs Autosomes - pairs 1-22 Sex chromosomes - 23rd pair determines
sex XX = girl XY = boy
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Questions
True or False: 1) Humans have 46 chromosomes 2) Humans have 23 pairs of
chromosomes 3) Pairs 1-23 are autosomes 4) XX is male 5) XY is male 6) Sister chromatids are copies of each
other. 7) Homologous chromosomes are
identical.
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MEIOSISMaking Sex Cells std. 2a-d, 2g
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2a. Chromosome Number
1. Chromosome Number Diploid (2n) – somatic (body)
cells with 2 sets of homologous chromosomes Humans = 46 chromosomes (23
pairs)
Haploid (n) – gamete sex cells that have 1 set of chromosomes Humans = 23 single chromosomes
Divide Box 2a into 2 columns (5 concepts to write, 3 in left column, 2 in right)
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Questions
1) A bug has a haploid number n=5. What is the diploid number (2n)?2) A crocodile has a diploid number 2n=50. What is the haploid number (n)?
meiosismeiosis
Haploid gamete
Diploid
Haploid gamete
2n
n n
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2a. Meiosis Overview
2. Meiosis vs. Mitosis Meiosis – cell division specific to sexual
reproduction that results in 4, genetically different, haploid gamete (sex) cells
Mitosis – asexual cell division that results in 2, genetically identical, diploid cells
Zygote
46
2323
First cell of a new organism
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Questions
A = Mitosis B = Meiosis C = Both 1) Type of cell division. 2) Produces 2 cells 3) Produces 4 cells 4) Sexual reproduction 5) Asexual reproduction 6) Resulting cells are genetically different 7) Diploid to haploid 8) Resulting cells are genetically identical 9) Diploid to Diploid
1)C2)A3)B4)B5)A6)B7)B8)A9)B
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2a. Steps of Meiosis
3. Steps of Meiosis Steps are mostly the same as Mitosis, but
diploid cell divides twice Meiosis I separates the homologous pairs Meiosis II separates sister chromatids
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Steps of Meiosis
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2a. Crossing Over
4. Crossing Over Homologous chromosomes
pair up and randomly trade piece of DNA during Prophase I
This creates genetic variation (new gene combinations that never existed before)
Draw Crossing Over (middle step in diagram)
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2a. Independent Assortment5. Independent Assortment Genes for different traits sort
independently into gametes Genes on different chromosomes are not
connected
Ex: The gene for eye color is not connected to the gene for hair color.
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Questions
What accounts for so many possible combinations of genes in gametes? A) Crossing-over B) Independent Assortment C) Both of these
C. Both
True or False: Meiosis 1 separates homologous pairs,
while Meiosis II separates sister chromatids. True!
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2b. Meiosis in Humans
Only gonads undergo meiosis MALES = the testis produces 4 sperm
(gametes) FEMALES = the ovaries produces 1 large
egg (gamete) and 3 polar bodies
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2c. Random Segregation
Law of Segregation It is random whether the gamete gets
the maternal or paternal version of each trait
Each gamete only gets one alleleDraw the diagram:Ex: If a pea plant is tall (Tt), half the gametes will have T and the other half will have t.
After fertilization, the offspring will have 2 alleles, one from mom & one from dad
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If a corn plant has a genotype of Ttyy, what are the possible genetic combinations that could be present in a single grain of pollen from this plant?
A. Ty, ty B. TY, ty C. TY, Ty, ty D. Ty, ty, tY, TY
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2d. Fertilization
Fertilization: Sperm + egg = zygote
(23) (23) (46) haploid + haploid = diploid
n + n = 2n
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2g. Predicting Offspring
Alleles - different versions of a trait Ex: Blue or brown eyes
Knowing the alleles of the parents allows you to predict the possible traits of the offspring.
Use a Punnett Square
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Questions
True or False? 1) Segregation occurs when genes
separate into gametes. 2) Females produce 4 eggs. 3) Males produce 4 sperm. 4) Fertilization occurs when diploid eggs
and sperm make a zygote. 5) Human zygotes have 23 chromosomes. 6) Alleles are different versions of a trait.
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PUNNETT SQUARESPredicting InheritanceStandard 3a & b
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3a. Punnett Squares
Vocabulary to know: Genotype = genes (TT, Tt, tt) Phenotype = appearance (Tall or short) Homozygous/Purebred = 2 of the same
allele (TT or tt) Heterozygous/Hybrid = 2 different alleles
(Tt)
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Types of Inheritance1. Autosomal – genes on
regular body chromosomes (#1-22)
Dominant covers up recessive ex: Bb The protein created by
the dominant gene functions better or is darker/stronger than the recessive protein
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2. Incomplete & Codominance Incomplete dominance =
show mix/blend between two alleles Ex: Red + White = pink
Codominance = show both traits at once Ex: Red + White = red &
white stripes
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3. Sex-linked – genes on X-chromosome Because men have
only 1 X (XY) they don’t have a “backup” X like women (XX) to hide the trait
If the X is bad, men have the disease Colorblindness,
hemophilia
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3b. Mendel’s Laws (see 2a & c) Mendel’s Law of Segregations – see 2c Mendel’s Law of Independent
Assortment – see 2a5