DNA RepairThe Ultimate Antiaging Tool

By: LifeSpan International, Inc. www.lifespaninternational.com

DNA IN THE “NEWS”DNA IN THE “NEWS”

On The Pill: Inner and Outer BeautyProducts; NY Post – March 6, 2005

The Science of Staying Young, Why We Age; Scientific American, Vol.14 # 3

CSI TV Program Making DNA Info Relevant

Diet & Genes, The New Science of Nutrition and Aging; Newsweek – February 7, 2005

DNA IN THE “NEWS”DNA IN THE “NEWS”

Clean Living…Dietary Supplements... the Future of Health Care: Forbes - November 29, 2004

How To Live To Be 100 (And Not Regret It); Time – August 30, 2004

A Four Year Study Measured Significant DNA Damage in Human and Animal Cells from Mobile Phone use; EU 2004

The Today Show, NBC National; “Stress Study”December 1, 2004 ( Dr. Sheldon Cohen /Elissa Epel) - University of California at San Francisco

NIH: DNA Damage Causes NIH: DNA Damage Causes AgingAging

Mutations

Replication errors

Persistent DNA damage

Genomicinstability

National Institutes of Health

Human Genome ProjectHuman Genome Project

Molecular Medicine

Antiaging Medicine

What is Aging?What is Aging?

“. . . the accumulation of random damage to the building blocks of life - especially to DNA, certain proteins, carbohydrates and lipids (fats) – that begins early in life and eventually exceeds the body’s self repair capabilities”

Leonard Hayflick, PhD., UCSF

S. Jay Olshanky, PhD., UICBruce Carnes, PhD., UIC

Scientific American, June 2002; 286(6):92-95

Why Do We Age?Why Do We Age?

Oxidative Stress

Damage to DNA (nucleus & mitochondria)

Poor DNA Repair

Aging and Aging Conditions

DNA Damage – Health DNA Damage – Health ConsequencesConsequences

DNA DAMAGEDNA DAMAGE

Mutagenic Diseases

e.g. cancer

Mutagenic Diseases

e.g. cancer

Toxic Disease

e.g. Inflammation

Toxic Disease

e.g. Inflammation

Two possible health consequences

The major cause of human disease has been identified as DNA damage by reactive oxygen species. This has been reviewed as a contributing factor for the origin and progression of a number of conditions including aging, autoimmunity, HIV infection and cancer.

Cellular Control of DNA RepairCellular Control of DNA Repair

DNA repair of damageDNA repair of damage

No diseaseNo disease

Mutagenic and toxic disease

Mutagenic and toxic disease

Incomplete Complete

Gene Deficiency

Biochemical Inhibition

Review of the cellular control of DNA damage

Results of Poor Results of Poor DNA RepairDNA Repair

DNA (gene) chemistry

Double stranded helical polymer

DNA damage caused by toxins, diet,

environment, radiation

Repair enzymes / Protein Processes

PARP, endonuclease, exonuclease, polymerase,

ligase

GOOD repair

BAD repair

GOOD

No disease

BAD

BAD REPAIR CASUES ALTERED GENE EXPRESSION, LEADS TO

CHRONIC DISEASES

Cancer, autoimmune, aging cardiovascular, inflammatory

BAD REPAIR CASUES ALTERED GENE EXPRESSION, LEADS TO

CHRONIC DISEASES

Cancer, autoimmune, aging cardiovascular, inflammatory

AC-11AC-11®®

INTRODUCING A “NEW” CLASS

OF

DIETARY SUPPLEMENTS

7 STEPS IN AGING7 STEPS IN AGING

YOUR ENVIRONMENT

(Poor diet, stress, pollution, smoking, exercise, sun exposure generate free radicals)

OXIDATIVE STRESS

GOOD GENE REPAIR

equals Antiaging

GENE DAMAGE

equals Aging

ALTERED GENE

EXPRESSION

Hobbled Proteins

(Glycation)

Wrong soup mix

(Mitochonria function)

Misfiring genetic switches

(Methylation and acetylation)

Immune glitches

(Inflammation)

Faulty cell communication

(Hormone imbalance)

Mitochondrial DNAMitochondrial DNA

A single ATP molecule must be recycled within a mitochondrion approximately

1,000 times per day for the body to maintain its energy supply

Quenching Reactive Oxygen Quenching Reactive Oxygen Species in the MitochondrionSpecies in the Mitochondrion

About 10% of the air we breathe is converted into free radicals in the mitochondria of our cells to produce the energy of life (ATP).

Hydrogen peroxide, hydroxyl radical, superoxide, lipid peroxide and singlet oxygen are metabolic mistakes of ATP production.

Mitochondria -Primary source of free radical production in cells.

AC-11®AC-11®

Water soluble extract of Uncaria tomentosa; 8% CAE’s*, less than 0.05% oxindole alkaloids

*Carboxy alkyl esters

AC-11® primary bioactive ingredient has AC-11® primary bioactive ingredient has been identifiedbeen identified Found in Shayman natural extractions of UncariaFound in Shayman natural extractions of UncariaQuinic acid/Quinic acid lactones are significantly more Quinic acid/Quinic acid lactones are significantly more bioactive when purified from AC-11®bioactive when purified from AC-11®

Aging Effects of Oxidative StressAging Effects of Oxidative Stress

Oxidative stress

Reduces apoptosis

Increases cell survival

Increases TNFalpha

Inflammation

anti-infection

NF-kB

Tumor toxicity

Decreases cell survivalal

Increases apoptosis

Decreases TNFalpha

Anti-inflammation

Enhances immune cells Increases

differentiation/function

Anti-oxidation

Increases DNA repair

Decreases DNA damage

Enhances immune cellsPro-oxidant

Increases DNA damage

Inhibits immune cells

ActivationInhibition AC-11™

Enhance DNA RepairEnhance DNA Repair

STEP 1.. DNA damage detected. Enzymes uncoil and unzip DNA. (NFk-B inhibition works here also by stopping free radicals that damage DNA)

STEP 2.. Uncoiled structure gives access to repair enzymes.

STEP 3.. Damaged or misplaced nucleotides are cut out by enzymes. (Active genes are repaired first, then less active genes)

STEP 4. New segments are moved into place and the seams are sealed by other enzymes.

STEP 1.. DNA damage detected. Enzymes uncoil and unzip DNA. (NFk-B inhibition works here also by stopping free radicals that damage DNA)

STEP 2.. Uncoiled structure gives access to repair enzymes.

STEP 3.. Damaged or misplaced nucleotides are cut out by enzymes. (Active genes are repaired first, then less active genes)

STEP 4. New segments are moved into place and the seams are sealed by other enzymes.

#4

#1

##2#2

#3

NF-kB / DNA repair-- control NF-kB / DNA repair-- control DNA damage and your health DNA damage and your health

Second primary source of free radicals is inflammation

NF-kB inhibition reduces this source

Regardless the source, DNA repair removes DNA damage

These processes are controlled by dietary factors

About AC-11®About AC-11®

Patented extract from the bark of Uncaria tomentosa vine

Patented extraction method that isolates the potent Carboxy Alkyl Esters which comprise the active ingredients in the bark

No high molecular weight alkaloids associated with adverse reactions

Sustainable Growth Project which is controlled by the Brazilian Environmental Institute (IBAMA)

Work projects are conducted under the Floravida

Institutes guidance

Unique FormulationUnique FormulationAC-11®AC-11® OTHER UT FORMULATIONSOTHER UT FORMULATIONS

Patented for DNA Repair and Immune Enhancement

Patented Aqueous Extraction Process

Inner Bark Uncaria tomentosa Water Soluble Carboxy Alkyl Esters Alkaloid Free Ethical Harvesting Produced under Pharma

GMP’s Proven Safety / Efficacy

Hydroalcoholic Tinctures, Decoctions and Solvent Extracts

Bark, Leaves &/or Root Uncaria guianensis Water Insoluble Tetracyclic Oxindole

Alkaloids Petracyclic Oxindole

Alkaloids Environmentally

Threatening Questionable Safety

Stability and Safety

Stability data > 5 years LD50 > 8000 mg/kg

– Our current recommended oral dosage is 350mg per day– Topical study doses range from 0.5% to 5.0% in

solution No known toxicity Stability in activity & color as an ingredient in

cosmetics Passes cosmetic micro-efficacy studies under

USP challenge test method DSHEA registered Prop 65 Compliant

AC-11® – AC-11® – DSHEA Allowed ClaimsDSHEA Allowed Claims

AC-11® helps enhance natural immunity

AC-11® helps enhance natural DNA repair process

AC-11® research validates it’s use to treat AC-11® research validates it’s use to treat DNA damage via NF-kB inhibition and DNA DNA damage via NF-kB inhibition and DNA

repair enhancementrepair enhancement

Induction of apoptosis and tumor inhibition; Anticancer Res., 18:1998

Enhanced DNA repair, immune function; J. Ethnopharm., 69:2000

Enhanced antibody response to vaccination; Phytomed., 8:2001

Human DNA repair enhancement; Phytomed., 8:2001

DNA repair enhanced by combination with niacin, carotenoids and zinc; Can. Detect Prev., 22:1998

AC-11® Research, AC-11® Research, cont’d.cont’d.

Serum thiols to estimate DNA repair; J. Antiaging Med., 3:2000

WBC elevation with no pathology or loss of immune function – proof of concept in mouse; Phytomed. , 2002

DNA repair as a primary molecular target for antiaging therapies; Antiaging Med., 2002

Anti-aging nutritional supplementwith and without AC-11; Anti-aging Med., 2002

Uncaria extract (AC-11) prolongs leukocyte survival; PhytMed., 2003

Uncaria Extract (AC-11) inhibits cell division and NF-kB activity; Interntl. Immunopharmacology, 2003

Strong IP ProtectionStrong IP Protection

AC-11® provides DNA repair from sun damage, lightens and blends skin tones

Patent issued for the extraction process of the rain forest botanical Uncaria tomentosa – This process results in a proprietary,

standardized extract.

Patent filed for a compound and method of use for AC-11®

Strong IP Protection, Strong IP Protection, continuedcontinued

Patent issued for Thiol blood test to measure repair status of DNA

Patent issued for compound and method of

use for Nicoplex™, a formulation with AC-11®, niacinamide and zinc. This synergistic combination has been proven to significantly improve DNA Repair.

AC-11® has demonstrated it’s positive benefits in both “Inner and Outer Beauty” products.

Synergistic To Nutrients AC-Synergistic To Nutrients AC-11®11®

Niacin (niacinamide): a substrate for DNA repair enzymes

Zinc: attaches to DNA repair enzymes, activating them

Carotenoids: scavenge oxy-radicals and enhance immune response

Edible mushroom extracts

Combination Products w/ AC-11®Combination Products w/ AC-11®i.e. i.e. Nicomed & NicoplexNicomed & Nicoplex

Synergistic Blends that Combine

AC-11® with Nutrients / AntioxidantsPatented Products

--------------------------------------------------------Age Manager – Multi-Vitamin Type with

AC-11®AGE Erasers

Scientific ValidationScientific Validation

Clinical Study using orally administered AC-11® *

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DNADAMAGE2

FREE RADICALDAMAGE1

DNA REPAIRENZYMES3

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UP 12%

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Summary Summary 5 Categories of AC-11® Activity 5 Categories of AC-11® Activity

DNA Repair

Anti-inflammatory

Tumor Inhibition

Immune Enhancement

Antioxidant

OPPORTUNITIESOPPORTUNITIES

Food Beverage / Water Dietary Supplements Cosmetics / Topical Skin Care Sports Nutrition Age Management / DNA Health Care---------------------------------------------------------------------------------------------------------------

Home Based Business Network Sales Affiliate Partnerships

DNA RepairThe Ultimate Antiaging Tool

by: LifeSpan International, Inc.

www.lifespaninternational.com