Dna Profiling Using Pcr Notes
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DNA Profiling using PCR Uses these properties of DNA: DNA contains exons and introns (non- coding areas) Non-coding DNA has repetitive sequences Some are short (2 – 6 base pairs ) microsatellites or STRs that can repeat up to 100 times Different people have different length STRs Some STRs are more variable (polymorphic) between people than others Polymorphisms are shared by 5 – 20% of individuals but targeting multiple known STRs gives unique combinations. The more loci, the more discrimination. NZ targets 10 STR sites (the most variable possible) STRs are allelic – you may inherit different ones from each parent (can be homozygous and heterozugous)
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DNA profiling using PCR student notes
Transcript of Dna Profiling Using Pcr Notes
DNA Profiling using PCR
Uses these properties of DNA:
DNA contains exons and introns (non-coding areas) Non-coding DNA has repetitive sequences Some are short (2 – 6 base pairs ) microsatellites or STRs that can
repeat up to 100 times Different people have different length STRs Some STRs are more variable (polymorphic) between people than
others Polymorphisms are shared by 5 – 20% of individuals but targeting
multiple known STRs gives unique combinations. The more loci, the more discrimination.
NZ targets 10 STR sites (the most variable possible) STRs are allelic – you may inherit different ones from each parent
(can be homozygous and heterozugous) Homozygous STRs will not separate out with gel electrophoresis
because they will be the same length; heterozygous STRs will make separate bands.
