DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which...
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Transcript of DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which...
![Page 1: DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which stores instructions for building the “machines” in.](https://reader030.fdocuments.in/reader030/viewer/2022032607/56649ed25503460f94be1c9f/html5/thumbnails/1.jpg)
DNA Fingerprinting
Class 832
![Page 2: DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which stores instructions for building the “machines” in.](https://reader030.fdocuments.in/reader030/viewer/2022032607/56649ed25503460f94be1c9f/html5/thumbnails/2.jpg)
LEGO Model of DNA•DNA is a molecule in your body.
•It is a code, which stores instructions for building the “machines” in your body.
•It is a double helix.
•It is a millionth of a millimeter wide.
•In humans, a molecule of DNA is 200,000,000 bases long.
![Page 3: DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which stores instructions for building the “machines” in.](https://reader030.fdocuments.in/reader030/viewer/2022032607/56649ed25503460f94be1c9f/html5/thumbnails/3.jpg)
DNA is a Code
GGAAAACTAACCCTTTTGATTG
•The genetic code is written in A’s, T’s, C’s and G’s.
•The sequence of A’s, T’s, C’s and G’s uniquely identifies a person, unless they are a twin.
•That’s why it’s called DNA fingerprinting.
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Restriction Enzymes are Molecules that Cut DNA at
Particular “Words”
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Restriction Enzyme Cuts Yield Different Sized DNA Fragments
GGATGGCCTAA/TTCCGGTTAA/TTGGTACCGGATT/AAGGCCAATT/AA
11 10 2
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Gel Electrophoresis is Used to Separate the Fragments Based on Size
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Example DNA Fingerprint
•Column in Gel corresponds to a person’s DNA.
•It is called a person’s DNA fingerprint.
•Row corresponds to DNA fragment size.
![Page 8: DNA Fingerprinting Class 832. LEGO Model of DNA DNA is a molecule in your body. It is a code, which stores instructions for building the “machines” in.](https://reader030.fdocuments.in/reader030/viewer/2022032607/56649ed25503460f94be1c9f/html5/thumbnails/8.jpg)
Different People Have Different Restriction Enzyme Cut Sites
Resulting in different band patterns on a gel.
1 2
Resulting in different sized DNA fragments.
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Who did it?
Solving a Murder Mystery Using
DNA Fingerprinting
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Given crime scene DNA and suspect DNA you will construct a gel.
You will then analyze the gel to find a match between the crime scene DNA and a suspect’s DNA.
You will use this information to make inferences about the murders.
Solving Our Murder Mystery Using DNA
Fingerprinting