Constrained thermal denaturation of DNA under xed linking ...
DNA Denaturation by: -Heat -Chemicals (e.g. formamide) -High pH
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DNA Denaturation by:
-Heat
-Chemicals (e.g. formamide)
-High pH
Melting Temperature is dependent on:
-Length of DNA (for short fragments)
-Base composition
Topic 2, Nucleic acids, genes, and genomes
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Kinetics of Renaturation
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highly repetitive DNA –very low Cot
repetitive DNA –low Cot
non-repetitive DNA –higher Cot
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0 2 4
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Yeast artificial chromosome
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Bacterial artificial chromosome
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Sequence tagged sites
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Mapping with STSs
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Figure 24.11
“Shotgun sequencing and BAC walking”
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Physical distance versus genetic map distance
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What explains the conservation of gene order over large evolutionary distance?
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Figure 1: Neurospora has a low proportion of genes in multigene families.
Figure 2: Neurospora possesses few highly similar genes.
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Figure 3: Example of lack of recent duplications in a Neurospora gene family (MFS sugar transporters).
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Figure 4: Correspondence between predicted RIP, methylation and repetitive DNA.
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Figure 5: Overview of major intracellular signaling pathways in Neurospora.
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